BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,357,018	A→G	5.9%	F53F (TTT→TTC)	PP_2950 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,357,018	0	A	G	5.9%	975.7 / 30.1	303	F53F (TTT→TTC)	PP_2950	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (152/133); new base G (15/3); total (167/136)
Fisher's exact test for biased strand distribution p-value = 1.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 312 total aligned reads displayed.

AGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTC  >  NC_002947/3356870‑3357167

    gCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTc                                                                                                                                                  <  2:6170312/150‑1 (MQ=255)

     cccGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCg                                                                                                                                                 <  1:6233908/150‑1 (MQ=255)

       cGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGc                                                                                                                                               >  1:5893172/1‑150 (MQ=255)

           tcgtcgTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACgg                                                                                                                                           <  1:2354739/150‑1 (MQ=255)

             gtcgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGtt                                                                                                                                         <  2:1478352/150‑1 (MQ=255)

             gtcgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGtt                                                                                                                                         <  2:7101438/150‑1 (MQ=255)

              tcgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTa                                                                                                                                        <  1:5998934/150‑1 (MQ=255)

               cgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTAc                                                                                                                                       <  1:8242526/150‑1 (MQ=255)

               cgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTAc                                                                                                                                       <  2:5220844/150‑1 (MQ=255)

                gtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACg                                                                                                                                      <  1:6123952/150‑1 (MQ=255)

                gtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACg                                                                                                                                      >  1:7316176/1‑150 (MQ=255)

                    cACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAt                                                                                                                                  <  1:8884079/150‑1 (MQ=255)

                     aCTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAtt                                                                                                                                 >  1:9079699/1‑150 (MQ=255)

                     aCTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAtt                                                                                                                                 >  1:1906008/1‑150 (MQ=255)

                       tCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                                               <  1:7441194/150‑1 (MQ=255)

                       tCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                                               <  1:1565984/150‑1 (MQ=255)

                         gTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGgt                                                                                                                             <  2:3535757/150‑1 (MQ=255)

                          tAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGgtg                                                                                                                            >  2:1769498/1‑150 (MQ=255)

                             gCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTcc                                                                                                                         >  2:8814638/1‑150 (MQ=255)

                                   cGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGta                                                                                                                   <  2:1155338/150‑1 (MQ=255)

                                    ggACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGtat                                                                                                                  >  2:3539453/1‑150 (MQ=255)

                                     gACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGtata                                                                                                                 <  1:7816880/150‑1 (MQ=255)

                                      aCCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAg                                                                                                                <  2:4302066/150‑1 (MQ=255)

                                      aCCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAg                                                                                                                <  1:2980798/150‑1 (MQ=255)

                                         gcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGcc                                                                                                             >  2:3884055/1‑150 (MQ=255)

                                          cgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCa                                                                                                            <  2:2429247/150‑1 (MQ=255)

                                           gATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAg                                                                                                           <  1:7570807/150‑1 (MQ=255)

                                           gATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAg                                                                                                           >  2:1280795/1‑150 (MQ=255)

                                            aTGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAgg                                                                                                          <  1:2463072/150‑1 (MQ=255)

                                                      cGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCtttt                                                                                                <  2:2835405/150‑1 (MQ=255)

                                                       ggTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                                               <  1:8368884/150‑1 (MQ=255)

                                                       ggTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                                               <  2:9032888/150‑1 (MQ=255)

                                                       ggTGAAGGCCCAGAGCTCTTTGATCAACCGAGAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                                               <  2:6387510/150‑1 (MQ=255)

                                                        gTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCg                                                                                              >  2:3747280/1‑150 (MQ=255)

                                                         tGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCgg                                                                                             <  2:5523737/150‑1 (MQ=255)

                                                                ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                      <  2:237990/150‑1 (MQ=255)

                                                                ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                      >  2:1855301/1‑150 (MQ=255)

                                                                ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                      >  1:101337/1‑150 (MQ=255)

                                                                ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                      >  2:420519/1‑150 (MQ=255)

                                                                ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                      >  2:4892600/1‑150 (MQ=255)

                                                                 cAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCgg                                                                                     <  1:1911628/150‑1 (MQ=255)

                                                                 cAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCgg                                                                                     <  2:8742533/150‑1 (MQ=255)

                                                                   gagCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCTGGTTCGCGGGt                                                                                   >  1:6982953/1‑150 (MQ=255)

                                                                       tctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTc                                                                               <  2:7648900/150‑1 (MQ=255)

                                                                         tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                                             >  1:8149253/1‑150 (MQ=255)

                                                                         tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                                             <  1:4584052/150‑1 (MQ=255)

                                                                           tGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGc                                                                           >  2:2270646/1‑150 (MQ=255)

                                                                              tCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCAt                                                                        >  1:5255898/1‑150 (MQ=255)

                                                                                aaCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATcc                                                                      >  2:8329773/1‑150 (MQ=255)

                                                                                 aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                                                     >  2:9142436/1‑150 (MQ=255)

                                                                                  ccGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTc                                                                    <  1:6461282/150‑1 (MQ=255)

                                                                                   cggtactCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTcc                                                                   <  1:7678563/144‑2 (MQ=255)

                                                                                       aGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGa                                                               <  2:6587529/150‑1 (MQ=255)

                                                                                         tCCAGTTCTTTCTTCAACTTGCGAGTTATGAAGTCACGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAGTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAga                                                             >  2:86514/1‑149 (MQ=255)

                                                                                          ccAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTc                                                            >  2:5882960/1‑150 (MQ=255)

                                                                                              ttctttctTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGAc                                                        >  1:4389425/1‑150 (MQ=255)

                                                                                                 tttcttCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtt                                                     >  1:2852182/1‑150 (MQ=255)

                                                                                                 cttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtt                                                     >  2:1425406/5‑150 (MQ=255)

                                                                                                  ttcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCttt                                                    <  1:4866419/150‑1 (MQ=255)

                                                                                                  ttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCttt                                                    >  2:7278749/4‑150 (MQ=255)

                                                                                                   tcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                                                   <  2:159913/150‑1 (MQ=255)

                                                                                                   tattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtttt                                                   <  2:77904/148‑1 (MQ=255)

                                                                                                     ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                                                 >  2:845976/1‑149 (MQ=255)

                                                                                                     ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                                                 >  2:7205101/1‑149 (MQ=255)

                                                                                                       ccACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAAt                                               >  1:3839940/1‑150 (MQ=255)

                                                                                                         aCTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCg                                             >  1:2893655/1‑150 (MQ=255)

                                                                                                           ttGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGcc                                           >  1:4100912/1‑150 (MQ=255)

                                                                                                                  ttAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTcgc                                    <  2:6832411/150‑1 (MQ=255)

                                                                                                                    aGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGa                                  <  1:6606815/150‑1 (MQ=255)

                                                                                                                    aGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGa                                  <  2:4391697/150‑1 (MQ=255)

                                                                                                                      gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                >  2:6693899/1‑150 (MQ=255)

                                                                                                                      gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                <  1:5171014/150‑1 (MQ=255)

                                                                                                                      gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACATTTTCAATCGCCGATTCGCGAGt                                >  1:4596436/1‑150 (MQ=255)

                                                                                                                       aaGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTg                               <  1:5624508/150‑1 (MQ=255)

                                                                                                                          tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                            <  2:4560067/150‑1 (MQ=255)

                                                                                                                          tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                            >  2:5455230/1‑150 (MQ=255)

                                                                                                                          tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                            <  2:2607102/150‑1 (MQ=255)

                                                                                                                          tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                            >  2:2047827/1‑150 (MQ=255)

                                                                                                                          tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                            <  1:2801528/150‑1 (MQ=255)

                                                                                                                           cccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag                           <  2:323400/150‑1 (MQ=255)

                                                                                                                           cccGAGCTTCTTCACGGTTGTTCGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag                           <  2:7902719/150‑1 (MQ=255)

                                                                                                                             cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagga                         >  2:692695/1‑150 (MQ=255)

                                                                                                                             cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagga                         <  1:4677061/150‑1 (MQ=255)

                                                                                                                             cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGGGAAagga                         >  1:5366386/1‑150 (MQ=255)

                                                                                                                              gAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAaggag                        <  1:8635875/150‑1 (MQ=255)

                                                                                                                              gAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGGGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAaggag                        >  2:4685849/1‑150 (MQ=255)

                                                                                                                                gCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAggagga                      <  1:3226551/150‑1 (MQ=255)

                                                                                                                                 cttcttCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGaa                     <  2:4058980/150‑1 (MQ=255)

                                                                                                                                    cttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTg                  <  1:8837469/150‑1 (MQ=255)

                                                                                                                                      tcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                <  2:2821490/150‑1 (MQ=255)

                                                                                                                                      tcACGGTTGTTAGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATACCAGCCAACCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                >  2:7572308/1‑150 (MQ=255)

                                                                                                                                            ttgttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGa          <  2:3082489/150‑1 (MQ=255)

                                                                                                                                            ttgttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGa          <  1:7234567/150‑1 (MQ=255)

                                                                                                                                              gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt        <  1:1234662/150‑1 (MQ=255)

                                                                                                                                              gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt        <  1:1800280/150‑1 (MQ=255)

                                                                                                                                              gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt        >  2:3423371/1‑150 (MQ=255)

                                                                                                                                              gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt        <  1:5006492/150‑1 (MQ=255)

                                                                                                                                                ttGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgtgt      <  2:9079699/150‑1 (MQ=255)

                                                                                                                                                    aaaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTc  <  1:8012028/150‑1 (MQ=255)

                                                                                                                                                    aaaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTc  >  1:8997268/1‑150 (MQ=255)

AGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTC  >  NC_002947/3356870‑3357167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`