BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,357,027	A→G	5.8%	R50R (CGT→CGC)	PP_2950 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,357,027	0	A	G	5.8%	1001.1 / 29.0	312	R50R (CGT→CGC)	PP_2950	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (157/136); new base G (15/3); total (173/139)
Fisher's exact test for biased strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 314 total aligned reads displayed.

GAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACAT  >  NC_002947/3356878‑3357176

     ctcgtGCCACTCGGAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGtt                                                                                                                                                  <  2:8411738/149‑1 (MQ=255)

      tcgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTa                                                                                                                                                 <  1:5998934/150‑1 (MQ=255)

        gtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACg                                                                                                                                               <  1:6123952/150‑1 (MQ=255)

        gtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACg                                                                                                                                               >  1:7316176/1‑150 (MQ=255)

            cACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAt                                                                                                                                           <  1:8884079/150‑1 (MQ=255)

             aCTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAtt                                                                                                                                          >  1:1906464/1‑150 (MQ=255)

              cTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTg                                                                                                                                         >  1:3071629/1‑150 (MQ=255)

               tCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                                                        <  2:3369344/150‑1 (MQ=255)

                         aaCGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCg                                                                                                                              >  2:7580482/1‑150 (MQ=255)

                          aCGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGAAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGt                                                                                                                             >  1:7977398/1‑150 (MQ=255)

                           cGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTATGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGta                                                                                                                            >  2:1565074/1‑150 (MQ=255)

                            ggACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGtat                                                                                                                           >  2:3539453/1‑150 (MQ=255)

                                cgcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGc                                                                                                                       >  1:2821490/1‑150 (MQ=255)

                                cgcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGc                                                                                                                       <  2:7396662/150‑1 (MQ=255)

                                  cgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCa                                                                                                                     >  1:3942210/1‑150 (MQ=255)

                                  cgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCa                                                                                                                     <  2:2428689/150‑1 (MQ=255)

                                   gATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAg                                                                                                                    <  1:7570807/150‑1 (MQ=255)

                                          ttGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATc                                                                                                             >  2:71658/1‑150 (MQ=255)

                                          ttGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGGTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCCTTGGGTGTCCACCGTATAGGCCAGGGCAATc                                                                                                             >  1:5159066/1‑150 (MQ=255)

                                              cGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCATACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCtttt                                                                                                         <  2:2835404/150‑1 (MQ=255)

                                               ggTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                                                        <  2:4255422/150‑1 (MQ=255)

                                               ggTGAAGGCCCAGAGCACATTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                                                        <  1:4771186/150‑1 (MQ=255)

                                                gTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCg                                                                                                       >  1:4968927/1‑150 (MQ=255)

                                                gTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCg                                                                                                       >  2:3747280/1‑150 (MQ=255)

                                                 tGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCgg                                                                                                      <  1:8414794/150‑1 (MQ=255)

                                                  gAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCggg                                                                                                     >  1:1812276/1‑150 (MQ=255)

                                                   aaGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTAGGGt                                                                                                    >  1:9165700/1‑150 (MQ=255)

                                                        ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                               >  2:1461457/1‑150 (MQ=255)

                                                        ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                               >  2:420494/1‑150 (MQ=255)

                                                        ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                               >  1:101337/1‑150 (MQ=255)

                                                         cAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCgg                                                                                              <  2:3448367/150‑1 (MQ=255)

                                                           gagCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCTGGTTCGCGGGt                                                                                            >  1:6982953/1‑150 (MQ=255)

                                                           gagCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGt                                                                                            >  1:2434523/1‑150 (MQ=255)

                                                             gCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTAt                                                                                          <  2:6900060/150‑1 (MQ=255)

                                                              ctctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTAtt                                                                                         >  2:4000554/1‑150 (MQ=255)

                                                               tctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTc                                                                                        >  1:7437472/1‑150 (MQ=255)

                                                                 tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGgt                                                                                                                                      >  1:9062882/1‑102 (MQ=255)

                                                                 tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                                                      >  1:4674835/1‑150 (MQ=255)

                                                                 tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                                                      <  1:4584052/150‑1 (MQ=255)

                                                                      tCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCAt                                                                                 >  2:6299684/1‑150 (MQ=255)

                                                                        aaCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATcc                                                                               >  1:2875830/1‑150 (MQ=255)

                                                                         aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                                                              >  2:9142436/1‑150 (MQ=255)

                                                                         aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                                                              >  2:1725348/1‑150 (MQ=255)

                                                                          ccGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTc                                                                             <  2:3837772/150‑1 (MQ=255)

                                                                          ccGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTc                                                                             <  1:6461282/150‑1 (MQ=255)

                                                                           cggtactCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTcc                                                                            <  1:7678563/144‑2 (MQ=255)

                                                                           cGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCa                                                                            >  2:6830836/1‑150 (MQ=255)

                                                                            gATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAg                                                                           >  2:8904025/1‑150 (MQ=255)

                                                                            caTAGTCCAGTTCTTTCTTCCACTTGCGAGTTACGAAGTCCCGAGCTTCTACACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAg                                                                           <  2:8271133/149‑1 (MQ=255)

                                                                                gTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAg                                                                       >  1:8265565/1‑150 (MQ=255)

                                                                                gTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAg                                                                       >  2:4383047/1‑150 (MQ=255)

                                                                                 tCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGt                                                                      <  1:7800667/150‑1 (MQ=255)

                                                                                   cAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCg                                                                    >  1:1934175/1‑150 (MQ=255)

                                                                                     gTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGa                                                                  <  2:5508275/150‑1 (MQ=255)

                                                                                         tttcttCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtt                                                              >  2:8635875/1‑150 (MQ=255)

                                                                                         tttcttCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtt                                                              <  2:2216847/150‑1 (MQ=255)

                                                                                          ttcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCttt                                                             <  1:2664431/150‑1 (MQ=255)

                                                                                            cttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTc                                                           >  2:5994744/1‑150 (MQ=255)

                                                                                             ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                                                          >  1:5175005/1‑149 (MQ=255)

                                                                                               ccACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAAt                                                        >  2:7858546/1‑150 (MQ=255)

                                                                                                cACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATc                                                       >  1:5771161/1‑150 (MQ=255)

                                                                                                cACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATc                                                       <  2:139986/150‑1 (MQ=255)

                                                                                                 aCTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCg                                                      >  2:4880557/1‑150 (MQ=255)

                                                                                                      cGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGAt                                                 <  2:5268225/150‑1 (MQ=255)

                                                                                                       gAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGAtt                                                >  2:6972451/1‑150 (MQ=255)

                                                                                                       gAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGAtt                                                >  2:2354668/1‑150 (MQ=255)

                                                                                                          ttAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTcgc                                             >  2:5006492/1‑150 (MQ=255)

                                                                                                            aGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGa                                           <  2:7766502/150‑1 (MQ=255)

                                                                                                            aGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGa                                           <  1:6606815/150‑1 (MQ=255)

                                                                                                              gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                                                            <  2:6636007/131‑1 (MQ=255)

                                                                                                              gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                         >  1:4596407/1‑150 (MQ=255)

                                                                                                              gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                         >  2:6524965/1‑150 (MQ=255)

                                                                                                              gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                         >  2:5529155/1‑150 (MQ=255)

                                                                                                                  tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                     <  2:2607102/150‑1 (MQ=255)

                                                                                                                  tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                     <  1:1712646/150‑1 (MQ=255)

                                                                                                                  tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                     <  2:4560067/150‑1 (MQ=255)

                                                                                                                  tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                     >  2:2047823/1‑150 (MQ=255)

                                                                                                                  tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                     <  2:8740834/150‑1 (MQ=255)

                                                                                                                   cccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag                                    <  2:323400/150‑1 (MQ=255)

                                                                                                                    ccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAggg                                                                                                                  <  1:4198699/71‑1 (MQ=255)

                                                                                                                    ccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTAt                                                                                          >  2:182504/1‑95 (MQ=255)

                                                                                                                    ccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagg                                   >  2:7271746/1‑150 (MQ=255)

                                                                                                                     cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagga                                  <  2:1270700/150‑1 (MQ=255)

                                                                                                                     cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGGGAAagga                                  >  1:5366386/1‑150 (MQ=255)

                                                                                                                      gAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAaggag                                 <  2:877221/150‑1 (MQ=255)

                                                                                                                      gAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAaggag                                 <  1:8635875/150‑1 (MQ=255)

                                                                                                                          ttcttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAg                             >  2:4608910/1‑150 (MQ=255)

                                                                                                                            cttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTg                           <  1:8837469/150‑1 (MQ=255)

                                                                                                                             ttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTgg                          >  1:345938/1‑150 (MQ=255)

                                                                                                                              tcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                         <  2:2821487/150‑1 (MQ=255)

                                                                                                                              tcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                         >  1:8172575/1‑150 (MQ=255)

                                                                                                                                    ttgttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGa                   <  2:3082489/150‑1 (MQ=255)

                                                                                                                                    ttgttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGa                   <  1:7234567/150‑1 (MQ=255)

                                                                                                                                      gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt                 <  1:1234662/150‑1 (MQ=255)

                                                                                                                                        ttGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgtgt               <  2:9079699/150‑1 (MQ=255)

                                                                                                                                            aaaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTc           >  1:2926160/1‑150 (MQ=255)

                                                                                                                                              aCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGt         >  1:6268789/1‑150 (MQ=255)

                                                                                                                                              aCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTAAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGt         <  1:8114256/150‑1 (MQ=255)

                                                                                                                                                tCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTg       >  1:8991381/1‑150 (MQ=255)

                                                                                                                                                  ggCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGa     <  1:5495868/150‑1 (MQ=255)

GAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACAT  >  NC_002947/3356878‑3357176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`