BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,357,042	G→C	6.0%	T45T (ACC→ACG)	PP_2950 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,357,042	0	G	C	6.0%	1010.5 / 38.1	301	T45T (ACC→ACG)	PP_2950	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (159/124); new base C (15/3); total (174/127)
Fisher's exact test for biased strand distribution p-value = 2.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 313 total aligned reads displayed.

TCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAAT  >  NC_002947/3356893‑3357191

tCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                                                                       <  2:3369344/150‑1 (MQ=255)

tCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                                                                       <  1:8963699/150‑1 (MQ=255)

  gTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGgt                                                                                                                                                     <  2:1320565/150‑1 (MQ=255)

   tAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGgtg                                                                                                                                                    >  2:1769498/1‑150 (MQ=255)

         gaaCGGACCGCGAGGCGGTGGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTGCTTCCACTTGCGAGTTAGTAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCAcc                                                                                                                                              <  2:6912048/149‑1 (MQ=255)

            cGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGta                                                                                                                                           <  2:2410153/150‑1 (MQ=255)

             ggACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTAGCGAGTTAGGAAGTACCGAGCTTCTTCAAGGTTGTTTGCAAACTCGGCACTGTTACTCCATTGGGTGTCCACCGtat                                                                                                                                          >  2:3539441/1‑150 (MQ=255)

                 cgcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                                                                       >  1:7263176/1‑133 (MQ=255)

                 cgcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                                                                       <  2:7263176/133‑1 (MQ=255)

                   cgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCa                                                                                                                                    <  1:5281499/150‑1 (MQ=255)

                   cgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCa                                                                                                                                    <  2:4435372/150‑1 (MQ=255)

                    gATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAg                                                                                                                                   <  1:3820981/150‑1 (MQ=255)

                    gATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAg                                                                                                                                   <  1:7570807/150‑1 (MQ=255)

                     aTGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAgg                                                                                                                                  <  2:4380418/150‑1 (MQ=255)

                           ttGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATc                                                                                                                            >  1:585772/1‑150 (MQ=255)

                            tGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCt                                                                                                                           <  2:2565744/150‑1 (MQ=255)

                               cGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCtttt                                                                                                                        <  2:2835405/150‑1 (MQ=255)

                                ggTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                                                                       <  2:4255422/150‑1 (MQ=255)

                                ggTGAAGGCCCAGAGCACATTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                                                                       <  1:4771186/150‑1 (MQ=255)

                                 gTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCg                                                                                                                      >  2:3747073/1‑150 (MQ=255)

                                  tGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCgg                                                                                                                     <  2:5523737/150‑1 (MQ=255)

                                   gAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCggg                                                                                                                    >  1:1812276/1‑150 (MQ=255)

                                    aaGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTAGGGt                                                                                                                   >  1:9165700/1‑150 (MQ=255)

                                         ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                              >  1:7934174/1‑150 (MQ=255)

                                         ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                              >  2:1461457/1‑150 (MQ=255)

                                         ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                              >  2:1855301/1‑150 (MQ=255)

                                         ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                              >  1:101337/1‑150 (MQ=255)

                                          cAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCgg                                                                                                             <  2:3448367/150‑1 (MQ=255)

                                            gagCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGt                                                                                                           >  1:2434523/1‑150 (MQ=255)

                                            gagCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGt                                                                                                           >  1:537008/1‑150 (MQ=255)

                                                tctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTc                                                                                                       >  1:7437472/1‑150 (MQ=255)

                                                tctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTc                                                                                                       <  2:7648900/150‑1 (MQ=255)

                                                  tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                                                                     >  1:4674835/1‑150 (MQ=255)

                                                       tCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCAt                                                                                                >  2:6299684/1‑150 (MQ=255)

                                                         aaCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATcc                                                                                              >  2:8329264/1‑150 (MQ=255)

                                                         aaCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATcc                                                                                              >  1:2875830/1‑150 (MQ=255)

                                                          aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                                                                             >  2:9142436/1‑150 (MQ=255)

                                                           ccGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTc                                                                                            <  1:6461282/150‑1 (MQ=255)

                                                            cggtactCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTcc                                                                                           <  1:7678563/144‑2 (MQ=255)

                                                            cggtactCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTcc                                                                                           <  2:2282083/144‑2 (MQ=255)

                                                            cGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCa                                                                                           >  2:6830836/1‑150 (MQ=255)

                                                             gATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAg                                                                                          >  2:8904025/1‑150 (MQ=255)

                                                                 gTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAg                                                                                      >  1:8265565/1‑150 (MQ=255)

                                                                  tCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAgg                                                                                     >  1:5070848/1‑149 (MQ=255)

                                                                    cAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCg                                                                                   >  1:1934175/1‑150 (MQ=255)

                                                                          tttcttCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtt                                                                             >  2:3821212/1‑150 (MQ=255)

                                                                          cttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtt                                                                             >  2:1425406/5‑150 (MQ=255)

                                                                           ttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCttt                                                                            >  2:3987359/4‑150 (MQ=255)

                                                                            tcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                                                                           <  2:159913/150‑1 (MQ=255)

                                                                            tcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                                                                           <  2:7090428/150‑1 (MQ=255)

                                                                            tattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtttt                                                                           <  2:77904/148‑1 (MQ=255)

                                                                              ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                                                                         >  2:845976/1‑149 (MQ=255)

                                                                              ttcCACTTGCGAGTTAGGAAGTCCAGAGCTTCTTCACGGTTGGTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGAATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGACATCCTCAGCGAGTCGGACCTTTTCa                                                                         >  1:8147628/1‑150 (MQ=255)

                                                                                  aCTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCg                                                                     >  2:4880557/1‑150 (MQ=255)

                                                                                           ttAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTcgc                                                            >  2:5006492/1‑150 (MQ=255)

                                                                                           ttAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTcgc                                                            <  1:4952041/150‑1 (MQ=255)

                                                                                               gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                                                                           <  2:6636007/131‑1 (MQ=255)

                                                                                               gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                                        >  2:6524965/1‑150 (MQ=255)

                                                                                               gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                                        >  1:4596407/1‑150 (MQ=255)

                                                                                               gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                                        <  1:5171175/150‑1 (MQ=255)

                                                                                                aaGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTg                                                       <  1:5624508/150‑1 (MQ=255)

                                                                                                   tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                                    <  1:1712646/150‑1 (MQ=255)

                                                                                                   tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                                    >  2:5455230/1‑150 (MQ=255)

                                                                                                   tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                                    <  1:2801528/150‑1 (MQ=255)

                                                                                                   tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                                    >  2:2047827/1‑150 (MQ=255)

                                                                                                    cccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag                                                   <  2:323400/150‑1 (MQ=255)

                                                                                                    cccGAGCTTCTTCACGGTTGTTCGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag                                                   <  2:7902719/150‑1 (MQ=255)

                                                                                                     ccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAggg                                                                                                                                 <  1:4198699/71‑1 (MQ=255)

                                                                                                     ccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAggg                                                                                                                                 >  2:4198699/1‑71 (MQ=255)

                                                                                                      cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagga                                                 <  1:8494567/150‑1 (MQ=255)

                                                                                                       gAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAaggag                                                <  2:877221/150‑1 (MQ=255)

                                                                                                       gAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGGGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAaggag                                                >  2:4685849/1‑150 (MQ=255)

                                                                                                         gCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAggagga                                              <  1:3226551/150‑1 (MQ=255)

                                                                                                             cttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTg                                          <  1:8837469/150‑1 (MQ=255)

                                                                                                              ttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTgg                                         >  1:719614/1‑150 (MQ=255)

                                                                                                               tcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                                        <  2:2821490/150‑1 (MQ=255)

                                                                                                               tcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                                        >  2:2760319/1‑150 (MQ=255)

                                                                                                               tcACGGTTGTTAGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATACCAGCCAACCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                                        >  2:7572308/1‑150 (MQ=255)

                                                                                                                 aCGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCgg                                      >  2:8671766/1‑150 (MQ=255)

                                                                                                                   ggTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGACGTGGCGGAc                                    >  1:4433971/1‑150 (MQ=255)

                                                                                                                   ggTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGAc                                    >  1:9035653/1‑150 (MQ=255)

                                                                                                                       gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt                                <  1:1234662/150‑1 (MQ=255)

                                                                                                                       gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt                                <  1:7740941/150‑1 (MQ=255)

                                                                                                                         ttGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgtgt                              <  2:9079699/150‑1 (MQ=255)

                                                                                                                         ttGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgtgt                              <  1:8764398/150‑1 (MQ=255)

                                                                                                                           gCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgtgtgt                            <  2:7316176/150‑1 (MQ=255)

                                                                                                                             aaaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTc                          <  1:8012039/150‑1 (MQ=255)

                                                                                                                             aaaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTc                          >  1:2926160/1‑150 (MQ=255)

                                                                                                                              aaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGAACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCg                         >  2:5861182/1‑150 (MQ=255)

                                                                                                                               aCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGt                        >  1:6268789/1‑150 (MQ=255)

                                                                                                                                    gCACGGTTGCGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGAc                   >  1:6602155/1‑150 (MQ=255)

                                                                                                                                     cACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACa                  <  2:4570344/150‑1 (MQ=255)

                                                                                                                                          ttACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACAtggtg             <  2:2659771/150‑1 (MQ=255)

                                                                                                                                              gCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGc         >  1:6850521/1‑150 (MQ=255)

                                                                                                                                              gCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGc         >  2:1187317/1‑150 (MQ=255)

                                                                                                                                                cATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTGCGTTGGACATGGTGGTGCCt       >  2:6121216/1‑150 (MQ=255)

                                                                                                                                                 aTTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTc      >  2:2898825/1‑150 (MQ=255)

                                                                                                                                                 aTTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTc      >  1:2534739/1‑150 (MQ=255)

                                                                                                                                                    gggTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCaa   >  1:3006075/1‑150 (MQ=255)

                                                                                                                                                    gggTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCaa   <  1:4000554/150‑1 (MQ=255)

TCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAAT  >  NC_002947/3356893‑3357191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`