BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,357,050	C→A	5.5%	V43L (GTG→TTG)	PP_2950 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,357,050	0	C	A	5.5%	1076.8 / 33.7	321	V43L (GTG→TTG)	PP_2950	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (174/129); new base A (15/3); total (189/132)
Fisher's exact test for biased strand distribution p-value = 4.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

AACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAATCTTTTGGA  >  NC_002947/3356903‑3357199

aaCGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCg                                                                                                                                                     <  2:6590294/150‑1 (MQ=255)

  cGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGta                                                                                                                                                   <  2:2410153/150‑1 (MQ=255)

   ggACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGtat                                                                                                                                                  >  1:8218471/1‑150 (MQ=255)

   ggACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTAGCGAGTTAGGAAGTACCGAGCTTCTTCAAGGTTGTTTGCAAACTCGGCACTGTTACTCCATTGGGTGTCCACCGtat                                                                                                                                                  >  2:3539441/1‑150 (MQ=255)

     aCCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAg                                                                                                                                                <  1:2980798/150‑1 (MQ=255)

     aCCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAg                                                                                                                                                <  2:4302066/150‑1 (MQ=255)

       cgcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGc                                                                                                                                              >  1:2821490/1‑150 (MQ=255)

        gcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGcc                                                                                                                                             <  2:6233632/150‑1 (MQ=255)

         cgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCa                                                                                                                                            >  1:3942210/1‑150 (MQ=255)

          gATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAg                                                                                                                                           >  2:1280795/1‑150 (MQ=255)

                 ttGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATc                                                                                                                                    >  2:71658/1‑150 (MQ=255)

                 ttGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGGTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCCTTGGGTGTCCACCGTATAGGCCAGGGCAATc                                                                                                                                    >  1:5159066/1‑150 (MQ=255)

                  tGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCt                                                                                                                                   <  2:2565744/150‑1 (MQ=255)

                       gTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCg                                                                                                                              >  2:3747280/1‑150 (MQ=255)

                       gTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGAATAGGCCAGGGCAATCTTTTCg                                                                                                                              >  1:6403435/1‑150 (MQ=255)

                         gAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCggg                                                                                                                            >  1:1812276/1‑150 (MQ=255)

                               ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                                      >  2:420494/1‑150 (MQ=255)

                               ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                                      >  2:4892600/1‑150 (MQ=255)

                               ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                                      >  1:101337/1‑150 (MQ=255)

                               ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                                                                                      >  1:7934174/1‑150 (MQ=255)

                                  gagCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCTGGTTCGCGGGt                                                                                                                   >  1:6982953/1‑150 (MQ=255)

                                    gCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTAt                                                                                                                 >  1:7413657/1‑150 (MQ=255)

                                     ctctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTAtt                                                                                                                >  2:4000554/1‑150 (MQ=255)

                                      tctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTc                                                                                                               <  2:7648900/150‑1 (MQ=255)

                                        tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                                                                             >  1:4674835/1‑150 (MQ=255)

                                        tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                                                                             >  1:5676858/1‑150 (MQ=255)

                                               aaCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATcc                                                                                                      >  2:8329264/1‑150 (MQ=255)

                                                aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                                                                                     >  2:9142436/1‑150 (MQ=255)

                                                aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                                                                                     >  2:3175923/1‑150 (MQ=255)

                                                 ccGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTc                                                                                                    <  2:3837772/150‑1 (MQ=255)

                                                  cggtactCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTcc                                                                                                   <  2:2282083/144‑2 (MQ=255)

                                                    gtactCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGc                                                                                                 >  1:7229481/5‑150 (MQ=255)

                                                       gTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAg                                                                                              >  2:4383047/1‑150 (MQ=255)

                                                        tCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAgg                                                                                             >  1:534974/1‑149 (MQ=255)

                                                            gTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGa                                                                                         <  2:5508275/150‑1 (MQ=255)

                                                             ttctttctTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGAc                                                                                        <  2:1819196/150‑1 (MQ=255)

                                                             ttctttctTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGAc                                                                                        >  1:4389425/1‑150 (MQ=255)

                                                              tctttcttCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGAcc                                                                                       <  1:5914775/150‑1 (MQ=255)

                                                                tttcttCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtt                                                                                     <  2:2216847/150‑1 (MQ=255)

                                                                cttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtt                                                                                     >  2:1619703/5‑150 (MQ=255)

                                                                cttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtt                                                                                     >  2:1425406/5‑150 (MQ=255)

                                                                 ttcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGTCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCttt                                                                                    <  1:7583285/150‑1 (MQ=255)

                                                                 ttcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCttt                                                                                    <  2:2830679/150‑1 (MQ=255)

                                                                 ttcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCttt                                                                                    <  1:2664431/150‑1 (MQ=255)

                                                                 ttcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCttt                                                                                    <  2:3440458/150‑1 (MQ=255)

                                                                 ttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCttt                                                                                    >  2:7278749/4‑150 (MQ=255)

                                                                  tattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtttt                                                                                   <  2:77904/148‑1 (MQ=255)

                                                                    ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                                                                                 >  1:5175005/1‑149 (MQ=255)

                                                                    ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                                                                                 >  2:845976/1‑149 (MQ=255)

                                                                    ttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCa                                                                                 <  2:452263/150‑1 (MQ=255)

                                                                    ttcCACTTGCGAGTTAGGAAGTCCAGAGCTTCTTCACGGTTGGTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGAATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGACATCCTCAGCGAGTCGGACCTTTTCa                                                                                 >  1:8147628/1‑150 (MQ=255)

                                                                        aCTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCg                                                                             >  1:2893655/1‑150 (MQ=255)

                                                                          ttGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGcc                                                                           >  1:4100912/1‑150 (MQ=255)

                                                                            gCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGa                                                                         >  2:8825412/1‑150 (MQ=255)

                                                                            gCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGa                                                                         >  2:1512059/1‑150 (MQ=255)

                                                                             cGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGag                                                                        >  1:2437606/1‑149 (MQ=255)

                                                                             cGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGAt                                                                        <  2:5268225/150‑1 (MQ=255)

                                                                              gAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGAtt                                                                       >  1:5477866/1‑150 (MQ=255)

                                                                                 ttAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTcgc                                                                    >  2:5006492/1‑150 (MQ=255)

                                                                                     gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                                                >  1:4595796/1‑150 (MQ=255)

                                                                                     gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt                                                                >  2:5529155/1‑150 (MQ=255)

                                                                                         tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                                            <  2:8740834/150‑1 (MQ=255)

                                                                                         tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa                                                            <  1:1712646/150‑1 (MQ=255)

                                                                                          cccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag                                                           >  2:6421660/1‑150 (MQ=255)

                                                                                          cccGAGCTTCTTCACGGTTGTTCGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag                                                           <  2:7902719/150‑1 (MQ=255)

                                                                                            cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagga                                                         >  2:692695/1‑150 (MQ=255)

                                                                                            cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagga                                                         <  1:8494567/150‑1 (MQ=255)

                                                                                                 ttcttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAg                                                    >  2:1933890/1‑150 (MQ=255)

                                                                                                   cttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTg                                                  <  1:8837469/150‑1 (MQ=255)

                                                                                                    ttcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTgg                                                 >  1:7530820/1‑150 (MQ=255)

                                                                                                     tcACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                                                >  2:2772944/1‑150 (MQ=255)

                                                                                                     tcACGGTTGTTAGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATACCAGCCAACCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGc                                                >  2:7572308/1‑150 (MQ=255)

                                                                                                      cACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCg                                               <  2:4599515/150‑1 (MQ=255)

                                                                                                             gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt                                        <  1:1234662/150‑1 (MQ=255)

                                                                                                             gttTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgt                                        <  1:5006492/150‑1 (MQ=255)

                                                                                                               ttGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgtgt                                      <  2:9079699/150‑1 (MQ=255)

                                                                                                               ttGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAgtgt                                      <  1:8764398/150‑1 (MQ=255)

                                                                                                                 ggaaaCTCGGCTCGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATGTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGGGAGTGATTGGAGGATGTGGCGGACGAgtgtgt                                    >  2:6817057/3‑150 (MQ=255)

                                                                                                                   aaaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTc                                  <  1:8012039/150‑1 (MQ=255)

                                                                                                                   aaaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTc                                  >  1:8997268/1‑150 (MQ=255)

                                                                                                                    aaCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGAACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCg                                 >  2:5861182/1‑150 (MQ=255)

                                                                                                                     aCTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGt                                >  1:6268789/1‑150 (MQ=255)

                                                                                                                       tCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTg                              >  1:7679383/1‑150 (MQ=255)

                                                                                                                          gCACGGTTGCGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGAc                           >  1:6602155/1‑150 (MQ=255)

                                                                                                                            aCGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACAt                         >  2:3297239/1‑150 (MQ=255)

                                                                                                                               gTTACGCCAGTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACAtggt                      >  1:2114349/1‑150 (MQ=255)

                                                                                                                                ttACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACAtggtg                     <  2:2659993/150‑1 (MQ=255)

                                                                                                                                  aCGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATggtggt                   <  1:4781069/150‑1 (MQ=255)

                                                                                                                                  aCGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATggtggt                   >  1:3218386/1‑150 (MQ=255)

                                                                                                                                    gCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGc                 >  1:6850521/1‑150 (MQ=255)

                                                                                                                                    gCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGc                 >  2:1187317/1‑150 (MQ=255)

                                                                                                                                       aTTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTc              <  2:8218471/150‑1 (MQ=255)

                                                                                                                                       aTTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACACGGTGGTGCCTc              <  2:2859537/150‑1 (MQ=255)

                                                                                                                                               tCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAATCttt      >  1:3450560/1‑150 (MQ=255)

                                                                                                                                               tCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAATCttt      >  2:4791107/1‑150 (MQ=255)

                                                                                                                                               tCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAATCttt      <  1:99371/150‑1 (MQ=255)

                                                                                                                                                 cACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAggagga                                                      >  2:5969025/1‑100 (MQ=255)

                                                                                                                                                 cACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACCTGGTGGTGCCTCCAATCTGTTg    >  2:7814335/1‑150 (MQ=255)

                                                                                                                                                   ccGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAg                                                                                                  <  2:6890400/54‑1 (MQ=255)

                                                                                                                                                   ccGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAATCTTTTGGa  <  1:4892600/150‑1 (MQ=255)

AACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGAGGAAGTGGCGGACGAGTGTGTTCGTTGGACATGGTGGTGCCTCCAATCTTTTGGA  >  NC_002947/3356903‑3357199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`