Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 244,785 G→A 7.1% R154K (AGG→AAG)  algP → transcriptional regulator AlgP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947244,7850GA 7.1% 574.1 / 20.5 201R154K (AGG→AAG) algPtranscriptional regulator AlgP
Reads supporting (aligned to +/- strand):  ref base G (84/100);  new base A (9/5);  total (95/106)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

ACTCAAACGCGATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCT  >  NC_002947/244636‑244933
                                                                                                                                                     |                                                                                                                                                    
aCTCAAACGCGATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAg                                                                                                                                                      >  2:8408179/1‑150 (MQ=255)
  tCAAACGCGATGCTCAGGACAGCCTCAAGCTGTATCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAggg                                                                                                                                                    >  1:3917479/1‑150 (MQ=255)
  tCAAACGCGATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAggg                                                                                                                                                    >  2:5954586/1‑150 (MQ=255)
   cAAACGCGATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGc                                                                                                                                                   <  2:5928497/150‑1 (MQ=255)
    aaaCGCGATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCg                                                                                                                                                  >  1:231975/1‑150 (MQ=255)
      aCGCGATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGAc                                                                                                                                                <  1:3238449/150‑1 (MQ=255)
          gATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCt                                                                                                                                            >  1:1840356/1‑150 (MQ=255)
          gATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCt                                                                                                                                            >  1:1840471/1‑150 (MQ=255)
              cTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTgccg                                                                                                                                        >  2:8066993/1‑150 (MQ=255)
               tCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTgccgc                                                                                                                                       >  2:6008453/1‑150 (MQ=255)
                  ggACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCaa                                                                                                                                    <  1:7774776/150‑1 (MQ=255)
                  ggACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCaa                                                                                                                                    <  2:6811417/150‑1 (MQ=255)
                   gACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAg                                                                                                                                   >  2:522697/1‑150 (MQ=255)
                    aCAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGc                                                                                                                                  <  1:5967002/150‑1 (MQ=255)
                     cAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGcc                                                                                                                                 >  2:7695360/1‑150 (MQ=255)
                     cAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGcc                                                                                                                                 >  2:1062919/1‑150 (MQ=255)
                     cAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGcc                                                                                                                                 >  2:8639122/1‑150 (MQ=255)
                        ccTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGc                                                                                                                              <  2:231975/150‑1 (MQ=255)
                        ccTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGc                                                                                                                              >  1:560442/1‑150 (MQ=255)
                        ccTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGc                                                                                                                              >  1:652453/1‑150 (MQ=255)
                         cTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCg                                                                                                                             >  1:7276069/1‑150 (MQ=255)
                         cTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCg                                                                                                                             >  1:8961199/1‑150 (MQ=255)
                         cTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCg                                                                                                                             <  2:5685520/150‑1 (MQ=255)
                         cTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCg                                                                                                                             >  2:7570156/1‑150 (MQ=255)
                          tCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCgg                                                                                                                            >  2:4898465/1‑150 (MQ=255)
                           cAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGc                                                                                                                           >  2:582829/1‑150 (MQ=255)
                            aaGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGcc                                                                                                                          >  2:8537942/1‑150 (MQ=255)
                                  gCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCa                                                                                                                    >  2:3175100/1‑150 (MQ=255)
                                  gCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCa                                                                                                                    <  2:7149780/150‑1 (MQ=255)
                                    tCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGc                                                                                                                  >  2:5012454/1‑150 (MQ=255)
                                    tCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCGGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGc                                                                                                                  >  2:4033807/1‑150 (MQ=255)
                                    tCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCAGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGc                                                                                                                  >  1:4479249/1‑150 (MQ=255)
                                           gtAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAg                                                                                                           <  2:8990786/150‑1 (MQ=255)
                                            tAGGTCAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAgg                                                                                                          <  1:7570156/150‑2 (MQ=255)
                                             aGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAgac                                                                                                         <  2:8915421/150‑1 (MQ=255)
                                               gTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAgacga                                                                                                       >  2:792482/1‑150 (MQ=255)
                                               gTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGACCAAACAAGCTCACAAgacta                                                                                                       >  1:8936888/1‑148 (MQ=255)
                                                    gTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCagc                                                                                                  <  2:4074782/150‑3 (MQ=255)
                                                    gTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACt                                                                                                  <  1:793037/150‑1 (MQ=255)
                                                        gcgAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCCCGACGACCCCTgccg                                                                                              >  2:4736249/1‑150 (MQ=255)
                                                        gcgAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGTCGACCACTgccg                                                                                              >  2:2216079/1‑150 (MQ=255)
                                                        gcgAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTgccg                                                                                              >  2:2833116/1‑150 (MQ=255)
                                                          gAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCg                                                                                                                             >  2:5132880/1‑117 (MQ=255)
                                                           aaGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCa                                                                                           <  1:8304184/150‑1 (MQ=255)
                                                            aGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCaa                                                                                          <  1:4072777/150‑1 (MQ=255)
                                                            aGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCaa                                                                                          <  1:4031118/150‑1 (MQ=255)
                                                               tGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGcc                                                                                       >  1:358240/1‑150 (MQ=255)
                                                                gCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCTAGGGAACCGCTGCTGCCAAGCCTGCGGCCAAAGCCGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCt                                                                                      <  2:3450247/150‑1 (MQ=255)
                                                                  aGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGc                                                                                    >  1:8653646/1‑150 (MQ=255)
                                                                     cAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGc                                                                                 <  1:2419803/150‑1 (MQ=255)
                                                                     cAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGc                                                                                 <  1:5497646/150‑1 (MQ=255)
                                                                          cccTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGTCCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAAc                                                                            <  1:3111202/150‑1 (MQ=255)
                                                                            cTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCaa                                                                                                            >  2:4414588/1‑116 (MQ=255)
                                                                               gATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCt                                                                       <  2:6767348/150‑1 (MQ=255)
                                                                               gATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCt                                                                       <  1:370020/150‑1 (MQ=255)
                                                                                 tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGc                                                                     <  2:6624004/150‑1 (MQ=255)
                                                                                 tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGc                                                                     >  2:5508031/1‑150 (MQ=255)
                                                                                   aaCGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGCCTGCCa                                                                   >  1:4773782/1‑150 (MQ=255)
                                                                                     cGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAg                                                                 <  1:8985798/150‑1 (MQ=255)
                                                                                       tGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGc                                                               <  2:7138554/150‑1 (MQ=255)
                                                                                         cGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGa                                                             <  1:2912366/150‑1 (MQ=255)
                                                                                           gCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCACTGCCGCCAAGCCTGCGGCCAAACCCGCTGCCAAGGCGAcc                                                           >  1:2192663/1‑150 (MQ=255)
                                                                                            ccAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCAGCCGCCAAGCGTGCGGCCAGACCGGCTGGCTGGGCGACCACTGCCGCCGCGCCTGCGGCCAAACCGGCTGCCGGGGCGAGCg                                                          >  2:1594135/1‑150 (MQ=11)
                                                                                             cAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGc                                                                                                               >  2:4306304/1‑96 (MQ=255)
                                                                                               aaCCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTg                                                       >  1:6721087/1‑150 (MQ=255)
                                                                                                 ccGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTgcc                                                     <  2:1730018/150‑1 (MQ=255)
                                                                                                 ccGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTgcc                                                     <  1:2556633/150‑1 (MQ=255)
                                                                                                  cGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTgccg                                                    <  2:6721720/150‑1 (MQ=255)
                                                                                                        ccAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCTAAGCCTGc                                                                                                                              >  1:8239313/1‑70 (MQ=255)
                                                                                                         cAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCGGCTGCCAGGGCGACCGCTGCCGCCaa                                                                                                                                    <  1:614955/63‑1 (MQ=255)
                                                                                                         cAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCaa                                                                                                                                    >  2:614955/1‑63 (MQ=255)
                                                                                                         cAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGCCGACCCCTGCAGCCATGCATGCGGCCACACCGGATGCCAAGGCGACCGCTgccgcccggcc                                             >  1:6841148/1‑145 (MQ=14)
                                                                                                         cAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGACAAGACGACCTCTGACGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCGAAccc                                             >  1:7849228/1‑150 (MQ=14)
                                                                                                            ggCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGc                                          <  1:5733424/150‑1 (MQ=255)
                                                                                                            ggCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGc                                          <  1:2747360/150‑1 (MQ=255)
                                                                                                             gCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGACGACCCCTGCCGCCAAGCCTGCGGCCAACCCGCCGGCCCAGGCGACCGCTGCCGCCAATCCTGCg                                         >  1:3516735/1‑150 (MQ=14)
                                                                                                             ccaaaggctgctGCGTAGCGAGCTTCCTAACCAGCTGCCTCGGGGACCGCGGCAGCCAAGCCTGCGGCCAAACCTGCTGCCAAGACGACCTCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCg                                         <  2:2529747/144‑1 (MQ=11)
                                                                                                               aaCCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCAGCCAAGCCTGCGGc                                       >  2:8663152/1‑150 (MQ=11)
                                                                                                                   gctgctGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCTAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCaaa                                   <  2:5176061/150‑1 (MQ=255)
                                                                                                                   gctgctGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCAGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCaaa                                   <  1:6821976/150‑1 (MQ=255)
                                                                                                                     tgctgcCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAAcc                                 <  1:6269044/150‑1 (MQ=255)
                                                                                                                        tgcCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGc                              <  2:5779394/150‑1 (MQ=255)
                                                                                                                         gcCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCt                             <  1:5111307/150‑1 (MQ=255)
                                                                                                                         gcCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCt                             <  1:5849189/150‑1 (MQ=255)
                                                                                                                          ccAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAg                                                                                                           >  1:1326128/1‑71 (MQ=39)
                                                                                                                            aaGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCCGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGACCAAACCGGCTGcc                          >  1:8355931/1‑150 (MQ=255)
                                                                                                                               ccTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAg                       >  1:3998010/1‑150 (MQ=255)
                                                                                                                               ccTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAg                       <  1:8537942/150‑1 (MQ=255)
                                                                                                                                cTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAgg                      <  2:2022822/150‑1 (MQ=255)
                                                                                                                                 tGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGc                     <  2:2886258/150‑1 (MQ=255)
                                                                                                                                   cGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGa                   <  1:4364883/150‑1 (MQ=255)
                                                                                                                                      ccAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCCAGACGACCACTGCCGCCAAGCCTGCGGACGACCCCGCTGCCTAGGCGACCGCTGCCGCCCATCCTGCGGCCCCACCGGCGGCCACGGCGACCg                >  1:8069041/1‑150 (MQ=14)
                                                                                                                                        aaaCCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGGCCGcg              >  2:6190333/1‑149 (MQ=255)
                                                                                                                                           ccAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCCCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTgcc           <  1:522697/150‑1 (MQ=255)
                                                                                                                                                tGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAg                                                                                                           <  1:1653879/49‑1 (MQ=38)
                                                                                                                                                 gCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCTCTGCCGCCAAGCCTGCGGCCAAACCGGCGGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAGACCGGCTGCCACGGCGAGCGCTGCGGCCCAg     >  1:3425860/1‑150 (MQ=11)
                                                                                                                                                 gCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAg     >  1:8039984/1‑150 (MQ=255)
                                                                                                                                                  ccAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCGAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGc    >  2:2363473/1‑150 (MQ=255)
                                                                                                                                                  ccAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGc    >  1:5169736/1‑150 (MQ=255)
                                                                                                                                                  ccAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCGGCCAAGTCGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCa                                                                   <  1:2010646/87‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACTCAAACGCGATGCTCAGGACAGCCTCAAGCTGGCTCAGGGTGTAGGTAAAGTACGCGAAGCTGCAGGCAAGGCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCT  >  NC_002947/244636‑244933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: