BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	14,899	G→A	5.5%	V524I (GTC→ATC)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	14,899	0	G	A	5.5%	869.9 / 24.6	274	V524I (GTC→ATC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (137/122); new base A (8/7); total (145/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 285 total aligned reads displayed.

CTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCG  >  NC_002947/14750‑15048

cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                                                                                                       >  2:5313788/1‑150 (MQ=255)

cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                                                                                                       <  1:1703313/150‑1 (MQ=255)

 tGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGt                                                                                                                                                      <  2:7736061/150‑1 (MQ=255)

      tGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGc                                                                                                                                                 <  1:2273544/150‑1 (MQ=255)

         gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                                                                                                                              >  2:1447800/1‑150 (MQ=255)

          cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                                                                                             <  1:4914488/150‑1 (MQ=255)

          cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                                                                                             <  2:4302789/150‑1 (MQ=255)

          cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                                                                                             <  2:4224887/150‑1 (MQ=255)

           tgtgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTCCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGc                                                                                                                                            >  2:1105445/1‑150 (MQ=255)

              ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                                                                                         <  2:1181980/150‑1 (MQ=255)

              ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                                                                                         <  2:1923065/150‑1 (MQ=255)

               gCATCGGCCGCGAGGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACa                                                                                                                                        >  2:6841266/1‑150 (MQ=255)

                        gcgAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTg                                                                                                                               <  2:2447884/150‑1 (MQ=255)

                         cgAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGc                                                                                                                              >  1:6039209/1‑150 (MQ=255)

                            agagTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCa                                                                                                                           <  2:6335927/150‑1 (MQ=255)

                                tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccg                                                                                                                       >  2:6495101/1‑150 (MQ=255)

                                   aatcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGccgccg                                                                                                                    <  2:8056397/144‑1 (MQ=255)

                                   aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                                                                                                    >  1:5566960/1‑150 (MQ=255)

                                    acaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCcgccgc                                                                                                                   <  1:6816554/150‑1 (MQ=255)

                                    acaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCcgccgc                                                                                                                   <  1:7554178/150‑1 (MQ=255)

                                     tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                                                                                                  >  1:2850627/5‑150 (MQ=255)

                                     caTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCt                                                                                                                  <  1:5275486/150‑1 (MQ=255)

                                      aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                                                                                                 >  2:3142616/1‑150 (MQ=255)

                                         gACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTaa                                                                                                              >  1:6411947/1‑149 (MQ=255)

                                           cAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATaa                                                                                                            <  2:7299176/150‑1 (MQ=255)

                                           cAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATaa                                                                                                            <  1:2656823/150‑1 (MQ=255)

                                           cAAACTGTGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                            <  1:6196243/150‑1 (MQ=255)

                                           cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                            <  2:5213467/150‑1 (MQ=255)

                                           cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTACTTCGTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                            <  1:3361349/150‑1 (MQ=255)

                                             aaCTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAgg                                                                                                          <  1:3259960/150‑1 (MQ=255)

                                             aaCTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAgg                                                                                                          >  1:301998/1‑150 (MQ=255)

                                                tGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGa                                                                                                       >  2:7213606/1‑150 (MQ=255)

                                                tGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGa                                                                                                       >  2:5557150/1‑150 (MQ=255)

                                                 gCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGaa                                                                                                      >  2:3470967/1‑150 (MQ=255)

                                                  cGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGaaa                                                                                                     <  1:7171441/150‑1 (MQ=255)

                                                   gatATCACAACATCAAAATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCAGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                                    >  2:2260637/3‑150 (MQ=255)

                                                   gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                                    >  1:6389589/1‑150 (MQ=255)

                                                      aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                                                                                                 <  1:5332642/150‑1 (MQ=255)

                                                      aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                                                                                                 <  1:797170/150‑1 (MQ=255)

                                                         acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGTGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACCAGGTGAAACGAGGCa                                                                                              >  2:8302251/1‑150 (MQ=255)

                                                         acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                              >  2:1577939/1‑150 (MQ=255)

                                                         acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                              >  2:1395459/1‑150 (MQ=255)

                                                         acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                              >  1:3932702/1‑150 (MQ=255)

                                                         acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                              >  1:3115567/1‑150 (MQ=255)

                                                          caacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCaa                                                                                             <  2:8614852/150‑1 (MQ=255)

                                                           aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                                                                                            >  1:7373952/1‑150 (MQ=255)

                                                           aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                                                                                            >  1:8636946/1‑150 (MQ=255)

                                                                catcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGa                                                                                       <  2:407007/150‑1 (MQ=255)

                                                                  tcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGc                                                                                     >  2:3948737/1‑150 (MQ=255)

                                                                   catcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCa                                                                                    <  2:4523111/150‑1 (MQ=255)

                                                                     tcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGt                                                                                  >  2:5487373/1‑150 (MQ=255)

                                                                     tcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGt                                                                                  <  1:7826326/150‑1 (MQ=255)

                                                                       atGGCCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTAc                                                                                >  1:4446642/1‑150 (MQ=255)

                                                                                gCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGAc                                                                       <  2:8171438/150‑1 (MQ=255)

                                                                                  tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                                     >  1:2809359/1‑150 (MQ=255)

                                                                                   gACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGAt                                                                    <  2:6455077/150‑1 (MQ=255)

                                                                                    aCGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATg                                                                   >  2:1746793/1‑150 (MQ=255)

                                                                                            gggtCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                                           <  2:2628063/147‑1 (MQ=255)

                                                                                            ggTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                                           <  2:5637739/150‑1 (MQ=255)

                                                                                                 gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                      >  2:5372593/1‑150 (MQ=255)

                                                                                                  cacaTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAagag                                                     >  2:5857303/1‑150 (MQ=255)

                                                                                                     aTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTAc                                                  >  1:5971216/1‑150 (MQ=255)

                                                                                                     aTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTAc                                                  >  1:8159295/1‑150 (MQ=255)

                                                                                                      tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                                 >  1:3433225/1‑150 (MQ=255)

                                                                                                      tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                                 <  1:5857303/150‑1 (MQ=255)

                                                                                                      tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                                 <  1:8680996/150‑1 (MQ=255)

                                                                                                       ccGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACAt                                                <  1:1414151/150‑1 (MQ=255)

                                                                                                         gTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGa                                              >  1:4609390/1‑150 (MQ=255)

                                                                                                             gctgctgctGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCa                                          <  2:4210648/150‑1 (MQ=255)

                                                                                                                gctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTc                                       <  2:2931466/150‑1 (MQ=255)

                                                                                                                     tgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGctc                                  >  1:9100389/1‑150 (MQ=255)

                                                                                                                      gACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                      <  1:4524665/129‑1 (MQ=255)

                                                                                                                       aCCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTg                                >  2:2822750/1‑150 (MQ=255)

                                                                                                                        ccTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTgg                               >  2:8817557/1‑150 (MQ=255)

                                                                                                                         cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                              >  2:3768538/1‑150 (MQ=255)

                                                                                                                         cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                              >  1:1001717/1‑150 (MQ=255)

                                                                                                                         cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                              >  2:7900397/1‑150 (MQ=255)

                                                                                                                         cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                              >  2:6254946/1‑150 (MQ=255)

                                                                                                                         cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                              >  1:893758/1‑150 (MQ=255)

                                                                                                                         cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                              >  2:4745214/1‑150 (MQ=255)

                                                                                                                          ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGTGGCAAGAAGGAGCAGTACATCATGGACGATGATGAGAGGGAAGAGTACATGACCCAGTCGGCTCTGGaa                             >  1:504463/1‑150 (MQ=255)

                                                                                                                          ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGaa                             >  1:3754307/1‑150 (MQ=255)

                                                                                                                             ttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGAt                          <  1:1823353/150‑1 (MQ=255)

                                                                                                                                ttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGcc                       >  1:6786138/1‑150 (MQ=255)

                                                                                                                                ttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGcc                       >  1:3693120/1‑150 (MQ=255)

                                                                                                                                 tcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCa                      <  1:4032189/150‑1 (MQ=255)

                                                                                                                                    gTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGcc                   <  2:8026198/150‑1 (MQ=255)

                                                                                                                                       aGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGc                >  1:7797572/1‑150 (MQ=255)

                                                                                                                                         cTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAc              <  2:2035271/150‑1 (MQ=255)

                                                                                                                                          tGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAcc             >  2:5739565/1‑150 (MQ=255)

                                                                                                                                          tGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAcc             >  2:8646903/1‑150 (MQ=255)

                                                                                                                                           gCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCt            <  1:1472909/150‑1 (MQ=255)

                                                                                                                                            ccGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTg           >  1:4300971/1‑150 (MQ=255)

                                                                                                                                            ccGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTg           <  2:1850596/150‑1 (MQ=255)

                                                                                                                                              ggAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAgg                                                                                                <  1:5485285/63‑1 (MQ=255)

                                                                                                                                              ggAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATTGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGa         >  1:8094231/1‑150 (MQ=255)

                                                                                                                                              ggAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTggt         <  1:1957563/150‑2 (MQ=255)

                                                                                                                                              ggAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGa         <  2:3894614/150‑1 (MQ=255)

                                                                                                                                                   tGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAAt    >  2:3199080/1‑150 (MQ=255)

                                                                                                                                                     gTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCg  >  2:6126949/1‑150 (MQ=255)

CTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCG  >  NC_002947/14750‑15048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`