Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 264,032 G→T 14.3% P261P (CCG→CCT)  gabD‑I → succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947264,0320GT14.3% 850.7 / 101.9 297P261P (CCG→CCT) gabD‑Isuccinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (147/107);  new base T (21/22);  total (168/129)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

AGCGCCGGCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGC  >  NC_002947/263883‑264181
                                                                                                                                                       |                                                                                                                                                     
aGCGCCGGCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCg                                                                                                                                                       <  1:6102617/150‑1 (MQ=255)
aGCGCCGGCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCg                                                                                                                                                       >  2:7606817/1‑150 (MQ=255)
 gcgcCGGCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGt                                                                                                                                                      <  1:8634705/150‑1 (MQ=255)
 gcgcCGGCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGt                                                                                                                                                      <  2:1679192/150‑1 (MQ=255)
   gcCGGCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTc                                                                                                                                                    <  2:5331281/150‑1 (MQ=255)
      ggCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATc                                                                                                                                                 >  1:5700023/1‑150 (MQ=255)
      ggCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATc                                                                                                                                                 <  1:6392381/150‑1 (MQ=255)
      ggCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATc                                                                                                                                                 <  1:6724024/150‑1 (MQ=255)
      ggCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATc                                                                                                                                                 <  2:6899342/150‑1 (MQ=255)
      ggCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATc                                                                                                                                                 <  2:2610371/150‑1 (MQ=255)
            gTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTc                                                                                                                                           <  2:6692879/150‑1 (MQ=255)
                  ggcgAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCgacgac                                                                                                                                     <  2:848184/150‑1 (MQ=255)
                           aCCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTg                                                                                                                            >  1:3816623/1‑150 (MQ=255)
                             cGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGa                                                                                                                          >  2:9051454/1‑150 (MQ=255)
                             cGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGa                                                                                                                          >  2:3344635/1‑150 (MQ=255)
                                  ccTCCCCGGTACGCAAGCTGTCCTTCCCCGGCTCGACCGAAAACGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGCAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAgg                                                                                                                     <  1:5159415/149‑1 (MQ=255)
                                  aCTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAgg                                                                                                                     <  2:4570944/150‑1 (MQ=255)
                                   cTCCCTGGTACTCATGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGAC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCACTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGc                                                                                                                    <  2:5427530/150‑1 (MQ=255)
                                   cTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGc                                                                                                                    >  1:3885252/1‑150 (MQ=255)
                                    tCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCg                                                                                                                   >  1:1971886/1‑150 (MQ=255)
                                    tCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCg                                                                                                                   <  1:5781865/150‑1 (MQ=255)
                                    tCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCg                                                                                                                   <  1:6198861/150‑1 (MQ=255)
                                    tCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCg                                                                                                                   >  1:6593384/1‑150 (MQ=255)
                                    tCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCg                                                                                                                   >  1:1758460/1‑150 (MQ=255)
                                    tCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCg                                                                                                                   >  1:3355780/1‑150 (MQ=255)
                                      tgtcgtgcGCAAGCTAACCTTCACCGGCTCGACCGAGGTCGG‑CGCCGAGCT‑CTATAAGCAGAGCGCGCCGACCATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGa                                                                                                                          <  2:6381049/134‑1 (MQ=255)
                                      tgtcgtgcGCAAGCTAACCTTCACCGGCTCGACCGAGGTCGG‑CGCCGAGCT‑CTATAAGCAGAGCGCGCCGACCATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGa                                                                                                                          <  2:3951660/134‑1 (MQ=255)
                                      ccTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGt                                                                                                                 >  2:7710931/1‑150 (MQ=255)
                                      ccTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGt                                                                                                                 >  2:8711859/1‑150 (MQ=255)
                                         ggTACGCAAGCTGTCCTTCACCGGCTCGACCGAGATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGa                                                                                                              <  2:6281008/150‑1 (MQ=255)
                                                aaGCTGTCCGTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGcgcg                                                                                                       <  2:8736040/150‑1 (MQ=255)
                                                    tttCCTTCACCGGCTCGACCGAAGTTGGTCGTC‑TTTTGATGGCGC‑AGTGTGCACCGACCATCAAGCGTATTTCACTGGAGCTTGGTGGCAACGCACCTTTCATCGTGTTTGATGATGCCGACATTGATGCTGCTGTCGATGGCGCGatga                                                                                                   >  1:9150441/3‑148 (MQ=255)
                                                    tttCCTTCACCGGCTCGACCGAAGTTGGTCGTC‑TTTTGATGGCGC‑AGTGTGCACCGACCATCAAGCGTATTTCACTGGAGCTTGGTGGCAACGCACCTTTCATCGTGTTTGATGATGCCGACATTGATGCTGCTGTCGATGGCGCGatga                                                                                                   >  2:5177361/3‑148 (MQ=255)
                                                    tttCCTTCACCGGCTCGACCGAAGTTGGTCGTC‑TTTTGATGGCGC‑AGTGTGCACCGACCATCAAGCGTATTTCACTGGAGCTTGGTGGCAACGCACCTTTCATCGTGTTTGATGATGCCGACATTGATGCTGCTGTCGATGGCGCGatga                                                                                                   >  1:4157750/3‑148 (MQ=255)
                                                    tGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGatca                                                                                                   >  1:8364709/1‑150 (MQ=255)
                                                      tCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGatcatc                                                                                                 >  2:1582404/1‑150 (MQ=255)
                                                        cTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCAtctc                                                                                               >  2:8072361/1‑150 (MQ=255)
                                                           cACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCaa                                                                                            <  1:7240577/150‑1 (MQ=255)
                                                           cACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCaa                                                                                            <  1:8088858/150‑1 (MQ=255)
                                                            aCCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAg                                                                                           >  1:2109567/1‑150 (MQ=255)
                                                               ggCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTAc                                                                                        >  2:3055064/1‑150 (MQ=255)
                                                                  tCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGt                                                                                     >  1:2748514/1‑150 (MQ=255)
                                                                     aCCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTaac                                                                                  <  1:106339/150‑1 (MQ=255)
                                                                        gAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTaacaac                                                                               >  1:3090277/1‑150 (MQ=255)
                                                                          aaTCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACgg                                                                             >  2:6694370/1‑150 (MQ=255)
                                                                             cGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCa                                                                          <  1:6956978/150‑1 (MQ=255)
                                                                               gTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGtt                                                                                                                                            >  2:3477802/1‑82 (MQ=255)
                                                                                   cc‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTg                                                                    >  2:5473108/1‑150 (MQ=255)
                                                                                    c‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGc                                                                   <  2:3146902/150‑1 (MQ=255)
                                                                                    c‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGc                                                                   >  1:5379786/1‑150 (MQ=255)
                                                                                    c‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTAGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGc                                                                   <  2:3542816/150‑1 (MQ=255)
                                                                                       gCTGTTTGAAG‑AATGCTCCATGGACATAAAGATGTTGTCCCTGGTGCTGGGTGTCAACTCCCCGTTAATCGTGTTCGACGACGCCGACCTGGACTAGGCGGTCTAGGTCGCGATAATCTCCAAGTACCGTAACATCGGCCAGACCTGCGt                                                                 <  2:373125/150‑1 (MQ=255)
                                                                                         tGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCt                                                               <  1:3532240/150‑1 (MQ=255)
                                                                                          gATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTg                                                              >  1:2354870/1‑150 (MQ=255)
                                                                                            tGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTgcg                                                            >  1:1281908/1‑150 (MQ=255)
                                                                                              gaag‑aaTGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGcc                                                          >  2:8231584/1‑150 (MQ=255)
                                                                                                       ctccAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTAt                                                  >  2:7068821/3‑150 (MQ=255)
                                                                                                        gcCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAgg                                                                                                            <  1:5320551/91‑1 (MQ=255)
                                                                                                         ccAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGc                                                                            >  2:5364639/1‑122 (MQ=255)
                                                                                                           aaGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCt                                                                                                                             <  1:4658925/71‑1 (MQ=255)
                                                                                                           aaGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCt                                                                                                                             >  2:4658925/1‑71 (MQ=255)
                                                                                                            cgaccATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTatg                                             <  1:5522749/146‑3 (MQ=255)
                                                                                                             ggACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGt                                            <  1:7606817/150‑1 (MQ=255)
                                                                                                              gACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTg                                           <  2:1524624/150‑1 (MQ=255)
                                                                                                              accATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGta                                           <  2:5569340/148‑2 (MQ=255)
                                                                                                                cATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACa                                         <  2:6083864/150‑1 (MQ=255)
                                                                                                                cATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACa                                         <  2:1031894/150‑1 (MQ=255)
                                                                                                                 aTCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAg                                        <  1:8791862/150‑1 (MQ=255)
                                                                                                                 aTCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAg                                        >  1:9072106/1‑150 (MQ=255)
                                                                                                                 aTCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAg                                        >  2:8532889/1‑150 (MQ=255)
                                                                                                                  tCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAgg                                       >  1:3618004/1‑150 (MQ=255)
                                                                                                                   cAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGa                                      >  2:7993556/1‑150 (MQ=255)
                                                                                                                   cAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGa                                      >  1:1529546/1‑150 (MQ=255)
                                                                                                                    aagaagGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGAc                                     >  1:7517039/1‑150 (MQ=255)
                                                                                                                    aagaagGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGAc                                     >  2:4388897/1‑150 (MQ=255)
                                                                                                                      gaagGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCa                                         <  1:3418915/144‑1 (MQ=255)
                                                                                                                         ggTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGt                                >  1:6337366/1‑150 (MQ=255)
                                                                                                                           tttCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCt                              >  2:1972993/1‑150 (MQ=255)
                                                                                                                             tCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTac                            <  2:8884488/150‑1 (MQ=255)
                                                                                                                               gcttgAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTatga                          >  2:7517857/5‑150 (MQ=255)
                                                                                                                               ccTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTacga                          >  1:4080875/1‑150 (MQ=255)
                                                                                                                               ccTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTacga                          >  1:1434778/1‑150 (MQ=255)
                                                                                                                               ccTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTacga                          >  1:7370079/1‑150 (MQ=255)
                                                                                                                                cTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTacgac                         >  1:84767/1‑150 (MQ=255)
                                                                                                                                   gAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGAcgcg                      <  2:5679760/150‑1 (MQ=255)
                                                                                                                                       ttggcggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCtttg                  >  1:2179255/6‑148 (MQ=255)
                                                                                                                                       tGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCg                  >  2:5765579/1‑150 (MQ=255)
                                                                                                                                        gggTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGc                 >  1:6465987/1‑150 (MQ=255)
                                                                                                                                         ggTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGcc                <  2:1333086/150‑1 (MQ=255)
                                                                                                                                          gTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCg               <  1:4992212/150‑1 (MQ=255)
                                                                                                                                           cggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCga              <  2:2291019/149‑1 (MQ=255)
                                                                                                                                            ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTat                                              >  2:294104/1‑116 (MQ=255)
                                                                                                                                            ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTat                                              <  1:294104/117‑2 (MQ=255)
                                                                                                                                             gCAATGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCgaga            <  1:1000220/150‑1 (MQ=255)
                                                                                                                                             gCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCgaga            <  1:1664972/150‑1 (MQ=255)
                                                                                                                                             gCAACGACCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCgaga            >  2:6498052/1‑150 (MQ=255)
                                                                                                                                                aCGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGc         <  2:3864575/150‑1 (MQ=255)
                                                                                                                                                  gCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTg       >  1:439683/1‑150 (MQ=255)
                                                                                                                                                    cccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGc     >  1:4800718/1‑150 (MQ=255)
                                                                                                                                                    cccGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGc     >  2:2448830/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
AGCGCCGGCGAAGTTGGCGGCGAACTGACCGGCAACTCCCTGGTACGCAAGCTGTCCTTCACCGGCTCGACCGAAATCGGTCGCC‑AGCTGATGGAAG‑AATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGC  >  NC_002947/263883‑264181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑