Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,975,785 A→T 6.0% A218A (GCT→GCA)  PP_3503 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,975,7850AT 6.0% 979.0 / 32.2 288A218A (GCT→GCA) PP_3503sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (137/133);  new base T (10/8);  total (147/141)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

ATGGGCTGGCTGCCACCCACCCGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGC  >  NC_002947/3975636‑3975933
                                                                                                                                                     |                                                                                                                                                    
    gctggctgCCACCCACCCGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCAc                                                                                                                                                  >  1:755177/1‑150 (MQ=255)
          tgCCACCCACCCGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTaa                                                                                                                                            >  1:7138345/1‑150 (MQ=255)
          tgCCACCCACCCGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTaa                                                                                                                                            <  1:2609267/150‑1 (MQ=255)
           gCCACCCACCCGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTaaa                                                                                                                                           <  1:994068/150‑1 (MQ=255)
               cccacccGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGgcg                                                                                                                                       <  2:3027704/150‑1 (MQ=255)
               cccacccGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGgcg                                                                                                                                       >  2:206213/1‑150 (MQ=255)
                ccacccGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGgcgc                                                                                                                                      >  2:7467150/1‑150 (MQ=255)
                ccacccGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGgcgc                                                                                                                                      >  1:4213620/1‑150 (MQ=255)
                ccacccGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGgcgc                                                                                                                                      <  2:6521236/150‑1 (MQ=255)
                  acccGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGccc                                                                                                                                    >  2:2517335/1‑150 (MQ=255)
                     cGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTtc                                                                                                                                 <  1:7272719/150‑1 (MQ=255)
                       cTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTtctc                                                                                                                               <  1:8806394/150‑1 (MQ=255)
                       cTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTtctc                                                                                                                               <  1:3624446/150‑1 (MQ=255)
                         cGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCAt                                                                                                                             <  1:6555247/150‑1 (MQ=255)
                          cctgtgCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATg                                                                                                                            >  2:3643065/3‑150 (MQ=255)
                                cTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGaa                                                                                                                      >  2:8427292/1‑150 (MQ=255)
                                  tctcCTTCAAGAAACGCAGCAAGTTGTCCTGAAATTCCAACGGCTGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCGGCTTCGATACGACCGGTCTTGCGCTGGTTAGCACCGGTAAAGGCGCCCTTCTCATGGACGAACa                                                                                                                    <  1:4961224/150‑1 (MQ=255)
                                  tctcCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACa                                                                                                                    >  1:5065898/1‑150 (MQ=255)
                                   ctgcTGCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACaa                                                                                                                   >  1:3374929/4‑149 (MQ=255)
                                    tcCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACatt                                                                                                                  <  1:7686272/150‑3 (MQ=255)
                                      cTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTc                                                                                                                <  2:8000028/150‑1 (MQ=255)
                                       tGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCACGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGACCAGGTCg                                                                                                               >  2:6984955/1‑150 (MQ=255)
                                       tGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCg                                                                                                               >  1:8104982/1‑150 (MQ=255)
                                       tGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCg                                                                                                               >  2:3057285/1‑150 (MQ=255)
                                       tGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCg                                                                                                               >  1:1854964/1‑150 (MQ=255)
                                       tGCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCg                                                                                                               <  2:1899793/150‑1 (MQ=255)
                                       tGCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCg                                                                                                               >  2:2734974/1‑150 (MQ=255)
                                        gCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCgg                                                                                                              >  1:5154167/1‑150 (MQ=255)
                                        gCAACACACGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCgg                                                                                                              >  2:4631183/1‑150 (MQ=255)
                                          aaGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGAc                                                                                                            >  2:1155571/1‑150 (MQ=255)
                                          aaGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGAAAAGGCGCCCTTCTCATGGCCGAACAGATCGGAc                                                                                                            >  1:5852322/1‑150 (MQ=255)
                                            cacaCGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCGGACtc                                                                                                          <  2:6055493/147‑2 (MQ=255)
                                             aaaCGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTgg                                                                                                         <  1:8188524/150‑1 (MQ=255)
                                              aaCGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGa                                                                                                        >  1:578925/1‑150 (MQ=255)
                                               aCGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGAt                                                                                                       >  2:1320549/1‑150 (MQ=255)
                                               aCGCAGCAAGCGCGTCTGCGCCTCCATTGGCATATCGCCGATTTCATCAAGAAACAGCGTGCCGCCATTGGCTTGTTCAAAACGTCCGCTTGAACGGTTCTGTGCGCCAGTAAAGGCACCCTTTTCATGGCCGAACAATTCGGACTCGAt                                                                                                       <  1:8164499/150‑1 (MQ=255)
                                                  cagcaAGTTTGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCaa                                                                                                    <  2:890841/150‑1 (MQ=255)
                                                  cagcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCaa                                                                                                    >  1:2155657/1‑150 (MQ=255)
                                                  cagcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCaa                                                                                                    <  2:5562707/150‑1 (MQ=255)
                                                  cagcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCaa                                                                                                    >  2:3609451/1‑150 (MQ=255)
                                                   agcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAg                                                                                                   >  2:8561027/1‑150 (MQ=255)
                                                    gcaAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGt                                                                                                  >  2:5699667/1‑150 (MQ=255)
                                                         ttGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCg                                                                                             <  2:3448667/150‑1 (MQ=255)
                                                          tGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCgg                                                                                            <  1:2767304/150‑1 (MQ=255)
                                                           ggCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCggg                                                                                           <  2:6665848/150‑1 (MQ=255)
                                                            gacTGGAATTCGATCGTCTGGTCTCCGATTTCATCATGAAAAAATGTGCCGCGATCGGCCGCTTCGATAGTCCCGGTCTTGGGCTGGTGTGCACATGTATAGGCGCCATTCTCATGTCCTAACTGTTCGGACTGGATCAAGTGCTCgggg                                                                                          <  2:7607527/148‑1 (MQ=255)
                                                             ccTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGa                                                                                         <  1:1250904/150‑1 (MQ=255)
                                                              cTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCgggg                                                                                          <  2:4187767/148‑1 (MQ=255)
                                                              cTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCgggg                                                                                          >  1:4187767/1‑148 (MQ=255)
                                                              cTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGAt                                                                                        <  1:1569439/150‑1 (MQ=255)
                                                               tGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATc                                                                                       >  1:2365648/1‑150 (MQ=255)
                                                                gCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCg                                                                                      >  2:8274883/1‑150 (MQ=255)
                                                                    ttCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGcccc                                                                                  <  2:672588/150‑1 (MQ=255)
                                                                       cAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCa                                                                               <  2:1723083/150‑1 (MQ=255)
                                                                           ggCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTg                                                                           >  1:8338064/1‑150 (MQ=255)
                                                                            gCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGa                                                                          <  1:4451683/150‑1 (MQ=255)
                                                                              aGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATc                                                                        <  2:4270737/150‑1 (MQ=255)
                                                                                gacGCCGATAACATCAAGAAACAATGAGCCGACATCGGACGCTTCGATACGCCCGCTCTTGAGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCAAGGCCGAACAGATCGGACTGGATCAAGTGCTCTGCTATAGCCCCCCAGTTGATagc                                                                      >  2:4581259/3‑150 (MQ=255)
                                                                                  cGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGa                                                                    <  2:8271668/150‑1 (MQ=255)
                                                                                  cGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGa                                                                    <  2:8804331/150‑1 (MQ=255)
                                                                                   gCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGAt                                                                   >  2:5021519/1‑150 (MQ=255)
                                                                                   gCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGAt                                                                   >  1:1711616/1‑150 (MQ=255)
                                                                                       aTTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAAc                                                               >  2:3313518/1‑150 (MQ=255)
                                                                                        tttCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACg                                                              >  1:4771845/1‑150 (MQ=255)
                                                                                         ttCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACgg                                                             <  1:3044729/150‑1 (MQ=255)
                                                                                         ttCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCAGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACgg                                                             <  2:3756091/150‑1 (MQ=255)
                                                                                          tcatcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgc                                                            <  2:2257921/150‑1 (MQ=255)
                                                                                           catcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgct                                                           >  1:8690540/1‑150 (MQ=255)
                                                                                            atcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgctg                                                          >  2:2363794/1‑150 (MQ=255)
                                                                                            atcaAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACAGGTAAAGGCGACCTTCACATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGgctg                                                          >  2:7308981/1‑150 (MQ=255)
                                                                                               aaGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTc                                                       <  1:6979790/150‑1 (MQ=255)
                                                                                               aaGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTc                                                       >  2:8567388/1‑150 (MQ=255)
                                                                                                aGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCg                                                      >  1:7990571/1‑150 (MQ=255)
                                                                                                aGAAACAATGTGCAGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCg                                                      >  1:1206368/1‑150 (MQ=255)
                                                                                                     cAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCg                                                 >  1:405216/1‑150 (MQ=255)
                                                                                                     cAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCg                                                 <  1:8427973/150‑1 (MQ=255)
                                                                                                           gccgccATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGa                                           <  1:1375120/150‑1 (MQ=255)
                                                                                                            ccgccATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGAc                                          >  2:5295863/1‑150 (MQ=255)
                                                                                                            ccgccATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGAc                                          >  1:587462/1‑150 (MQ=255)
                                                                                                             cgccATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACt                                         >  2:290290/1‑150 (MQ=255)
                                                                                                               ccATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTgg                                       >  2:2479897/1‑150 (MQ=255)
                                                                                                                   cGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGt                                   <  1:7381522/150‑1 (MQ=255)
                                                                                                                      ccGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCa                                >  1:5760889/1‑150 (MQ=255)
                                                                                                                        gCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAg                              <  2:1083989/150‑1 (MQ=255)
                                                                                                                        gCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAg                              <  1:5323881/150‑1 (MQ=255)
                                                                                                                         cTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAgg                             <  1:3808221/150‑1 (MQ=255)
                                                                                                                           tCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTg                           <  1:6434644/150‑1 (MQ=255)
                                                                                                                              aTACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTgcgc                        <  1:2641324/150‑1 (MQ=255)
                                                                                                                              aTACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTgcgc                        <  1:8912848/150‑1 (MQ=255)
                                                                                                                                 cGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGcc                     <  2:9015638/150‑1 (MQ=255)
                                                                                                                                 cGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGcc                     <  1:6723429/150‑1 (MQ=255)
                                                                                                                                  gcgcGGTCGTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGTCCGAACAGTTCGGACTTGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCTCGATGAACGGCTGCTCGCTTCGTTGCGTCTGGCGGTGCAAGGTGCGCGgca                    <  2:5376090/147‑1 (MQ=255)
                                                                                                                                       gTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGc               <  1:1069282/150‑1 (MQ=255)
                                                                                                                                        tCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTACTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGct              >  1:9108408/1‑150 (MQ=255)
                                                                                                                                          ttGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTACTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGctct            >  2:6182761/1‑150 (MQ=255)
                                                                                                                                            gcgcTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCttt          <  1:2517335/150‑1 (MQ=255)
                                                                                                                                             cgcTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTg         >  2:5792183/1‑150 (MQ=255)
                                                                                                                                               cTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGcc       <  2:4370520/150‑1 (MQ=255)
                                                                                                                                               cTGGTGAGCACCGGTAAAGGCGCCCTTCTCATCGCCGAACAGATCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCACTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCATTGcc       <  2:7138345/150‑1 (MQ=255)
                                                                                                                                                    gAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGc  >  1:4695753/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATGGGCTGGCTGCCACCCACCCGCTCGATGTGCTTCTCCTGCAAGAAACGCAGCAAGTTGGCCTGCAATTCCAACGGCAGGTCGCCGATTTCATCAAGAAACAATGTGCCGCCATCGGCCGCTTCGATACGCCCGGTCTTGCGCTGGTGAGCACCGGTAAAGGCGCCCTTCTCATGGCCGAACAGTTCGGACTGGATCAAGTGCTCGGGGATCGCCCCGCAGTTGATCGCGATGAACGGCTGCTCGCTGCGTTGCGACTGGCGGTGCAAGGTGCGCGCCACCAGCTCTTTGCCGGTGC  >  NC_002947/3975636‑3975933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑