BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,135,943	T→A	5.1%	A351A (GCA→GCT) ‡	PP_3638 ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,135,943	0	T	A	5.1%	1275.0 / 33.6	372	A351A (GCA→GCT) ‡	PP_3638	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (218/133); new base A (8/12); total (226/146)
Fisher's exact test for biased strand distribution p-value = 5.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 394 total aligned reads displayed.

GCCCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGC  >  NC_002947/4135794‑4136092

   cacaCCACTCGGGCCAACGATGCTCACGACCTCACCACGTCCCAGCTGCAGGACGAACTGCGCCACCACCTCCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGAAGTCACGGCCCTCCAGCAACACCGCGCACATGCAACgccagcc                                                                                                                                                    >  2:286008/1‑150 (MQ=255)

    acacCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACgcctgcct                                                                                                                                                   >  1:920183/1‑150 (MQ=255)

       ccacTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGc                                                                                                                                                <  1:5836294/150‑1 (MQ=255)

          cTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCCCGCGGGTAGCCCCAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGtt                                                                                                                                             <  2:6621941/150‑1 (MQ=255)

           tGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTg                                                                                                                                            <  2:6227299/150‑1 (MQ=255)

            ggggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGc                                                                                                                                           <  2:4185921/150‑1 (MQ=255)

            ggggCCAAGGATGCTCACGACCTCACCAGGGGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCACGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGc                                                                                                                                           <  1:5116554/150‑1 (MQ=255)

             gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCa                                                                                                                                          >  2:4124396/1‑150 (MQ=255)

             gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCa                                                                                                                                          >  1:449440/1‑150 (MQ=255)

              ggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCat                                                                                                                                         <  1:1428376/150‑2 (MQ=255)

               gCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGt                                                                                                                                        >  2:1338439/1‑150 (MQ=255)

                ccAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCatga                                                                                                                                       <  1:6852749/150‑4 (MQ=255)

                 cAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTc                                                                                                                                      >  1:1788465/1‑150 (MQ=255)

                 cAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCatgag                                                                                                                                      <  1:1272559/150‑5 (MQ=255)

                  aaGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGCACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCg                                                                                                                                     <  2:7651653/150‑1 (MQ=255)

                  aaGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCg                                                                                                                                     <  2:2451712/150‑1 (MQ=255)

                   aGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCgg                                                                                                                                    >  2:4787438/1‑150 (MQ=255)

                    ggATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGgc                                                                                                                                   >  2:2362247/1‑150 (MQ=255)

                    ggATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGgc                                                                                                                                   <  2:3016875/150‑1 (MQ=255)

                      aTGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGgcgc                                                                                                                                 <  1:819860/150‑1 (MQ=255)

                      aTGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGgcgc                                                                                                                                 <  1:5260094/150‑1 (MQ=255)

                          tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCaa                                                                                                                             >  1:256911/1‑150 (MQ=255)

                          tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCaa                                                                                                                             >  2:2748666/1‑150 (MQ=255)

                            aCGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAAtt                                                                                                                           >  2:8334529/1‑150 (MQ=255)

                                  tCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCa                                                                                                                     <  1:6745397/150‑1 (MQ=255)

                                  tCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCa                                                                                                                     >  2:3774961/1‑150 (MQ=255)

                                  tCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCa                                                                                                                     >  2:172393/1‑150 (MQ=255)

                                      cTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCt                                                                                                                 >  2:8571571/1‑150 (MQ=255)

                                      cTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCt                                                                                                                 >  1:7248308/1‑150 (MQ=255)

                                          tGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGc                                                                                                             <  2:109461/150‑1 (MQ=255)

                                            gcAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGt                                                                                                           >  1:663334/2‑150 (MQ=255)

                                            ccAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGt                                                                                                           >  2:5703052/1‑150 (MQ=255)

                                              aGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                         <  1:5910009/150‑1 (MQ=255)

                                               gCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGAc                                                                                                        <  2:3401291/150‑1 (MQ=255)

                                                cTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACg                                                                                                       >  2:810447/1‑150 (MQ=255)

                                                 tGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGa                                                                                                      >  1:6444352/1‑150 (MQ=255)

                                                      gTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGc                                                                                                 <  1:78339/150‑1 (MQ=255)

                                                      gTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGc                                                                                                 >  1:7554217/1‑150 (MQ=255)

                                                          aaCTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGa                                                                                             >  2:6578893/1‑150 (MQ=255)

                                                              gcgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCACTTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGc                                                                                         <  1:2331847/150‑1 (MQ=255)

                                                              gcgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGc                                                                                         <  1:947270/150‑1 (MQ=255)

                                                               cgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGcc                                                                                        >  1:1002446/1‑150 (MQ=255)

                                                                  cAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGAc                                                                                     >  1:5036456/1‑150 (MQ=255)

                                                                        atgctgcCAACCACCCACACGCGGGTAGACCAAGCGGATGTCACGGGCCTCCAGCAACAACGCGCTCAAGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGc                                                                               >  2:5123721/2‑150 (MQ=255)

                                                                               cAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                        >  2:1144325/1‑150 (MQ=255)

                                                                               cAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                        <  2:1522759/150‑1 (MQ=255)

                                                                                aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcg                                                                       >  1:1950565/1‑150 (MQ=255)

                                                                                  ccaccCTCCCGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCGGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCggg                                                                     <  2:4718504/150‑1 (MQ=255)

                                                                                  ccaccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCggg                                                                     >  1:4771926/1‑150 (MQ=255)

                                                                                   caccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGc                                                                    >  1:5502931/1‑150 (MQ=255)

                                                                                    accCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCa                                                                   >  1:4645036/1‑150 (MQ=255)

                                                                                      ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                 <  1:8143350/150‑1 (MQ=255)

                                                                                      ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                 >  2:7364423/1‑150 (MQ=255)

                                                                                       cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATc                                                                                                    <  2:6984226/114‑1 (MQ=255)

                                                                                       cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAAc                                                                >  1:5081137/1‑150 (MQ=255)

                                                                                               ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGcc                                                        >  2:6353313/1‑150 (MQ=255)

                                                                                               ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGcc                                                        <  2:8959721/150‑1 (MQ=255)

                                                                                               ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGcc                                                        >  1:2741791/1‑150 (MQ=255)

                                                                                                     ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                                  >  1:3352169/1‑150 (MQ=255)

                                                                                                     ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                                  >  1:8345441/1‑150 (MQ=255)

                                                                                                     ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                                  >  2:5890452/1‑150 (MQ=255)

                                                                                                      cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                 <  2:8468256/150‑1 (MQ=255)

                                                                                                      cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                 >  2:6065630/1‑150 (MQ=255)

                                                                                                       aaGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTgg                                                                                                               >  2:3961264/1‑87 (MQ=255)

                                                                                                          cGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCaga                                             <  2:6444352/150‑1 (MQ=255)

                                                                                                          cGGATGTCACGGGCCTCCAGCAACACCGCGCTCATCCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCaga                                             <  2:7901341/150‑1 (MQ=255)

                                                                                                           ggATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCagag                                            <  1:1143736/150‑1 (MQ=255)

                                                                                                           ggATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCagag                                            >  1:454233/1‑150 (MQ=255)

                                                                                                            gATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAgg                                           >  1:6183184/1‑150 (MQ=255)

                                                                                                                  aCGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGcc                                     >  2:3508864/1‑150 (MQ=255)

                                                                                                                     ggCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTg                                  <  1:2748779/150‑1 (MQ=255)

                                                                                                                      gCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgc                                 <  2:449440/150‑1 (MQ=255)

                                                                                                                      gCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgc                                 >  1:2105457/1‑150 (MQ=255)

                                                                                                                        cTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgccg                               >  2:2539709/1‑150 (MQ=255)

                                                                                                                         tCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgccgc                              <  2:3276861/150‑1 (MQ=255)

                                                                                                                         tCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCACCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgccgc                              >  2:5562520/1‑150 (MQ=255)

                                                                                                                            aGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCAc                           >  1:1652532/1‑150 (MQ=255)

                                                                                                                             gCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACt                          >  1:3935202/1‑150 (MQ=255)

                                                                                                                              cAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTg                         <  1:4931666/150‑1 (MQ=255)

                                                                                                                               aaCACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTgg                        >  1:7120701/1‑150 (MQ=255)

                                                                                                                                 cacCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCACGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGcc                      >  1:4014694/1‑150 (MQ=255)

                                                                                                                                  acCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCt                     <  2:1413776/150‑1 (MQ=255)

                                                                                                                                  acCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCt                     >  1:9028513/1‑150 (MQ=255)

                                                                                                                                    cgcgCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGc                   >  1:8628471/1‑150 (MQ=255)

                                                                                                                                    cgcgCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGc                   >  2:3109811/1‑150 (MQ=255)

                                                                                                                                         tCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTc              >  2:3187475/1‑150 (MQ=255)

                                                                                                                                           aTGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa            <  1:3737902/150‑1 (MQ=255)

                                                                                                                                           aTGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa            >  1:1566781/1‑150 (MQ=255)

                                                                                                                                            ttgcgcGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg           >  2:8754084/6‑150 (MQ=255)

                                                                                                                                            tGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg           <  2:2626372/150‑1 (MQ=255)

                                                                                                                                            tGCAACGCCAGGCAGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg           >  1:6516879/1‑150 (MQ=255)

                                                                                                                                            tGCAACGCCAGGCAGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg           >  1:2960351/1‑150 (MQ=255)

                                                                                                                                            tGCAACGCCAGGCAGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg           >  1:8814400/1‑150 (MQ=255)

                                                                                                                                             gCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGt          <  2:1788465/150‑1 (MQ=255)

                                                                                                                                              cAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCATAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt         <  1:8459186/150‑1 (MQ=255)

                                                                                                                                               aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt         <  2:6747341/149‑1 (MQ=255)

                                                                                                                                               aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTg        >  1:3216879/1‑150 (MQ=255)

                                                                                                                                                  gcctgcctGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCt                     >  1:3606299/1‑134 (MQ=255)

                                                                                                                                                  gcctgcctGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTAc     >  1:6933128/1‑150 (MQ=255)

                                                                                                                                                     tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACcgc  >  2:4931936/1‑150 (MQ=255)

GCCCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGC  >  NC_002947/4135794‑4136092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`