BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,135,945	C→G	5.4%	A351P (GCA→CCA) ‡	PP_3638 ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,135,945	0	C	G	5.4%	1261.6 / 42.9	372	A351P (GCA→CCA) ‡	PP_3638	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (218/134); new base G (8/12); total (226/146)
Fisher's exact test for biased strand distribution p-value = 6.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 390 total aligned reads displayed.

CCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGCGT  >  NC_002947/4135796‑4136094

       acTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGt                                                                                                                                                <  1:2614059/150‑1 (MQ=255)

        cTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGtt                                                                                                                                               <  1:5039533/150‑1 (MQ=255)

          ggggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGc                                                                                                                                             <  2:6468572/150‑1 (MQ=255)

          ggggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGc                                                                                                                                             <  2:101592/150‑1 (MQ=255)

            ggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAg                                                                                                                                           >  2:5581819/1‑150 (MQ=255)

            ggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCat                                                                                                                                           <  1:1428376/150‑2 (MQ=255)

              ccAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGtt                                                                                                                                         >  2:4524844/1‑150 (MQ=255)

               cAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCatgag                                                                                                                                        <  1:1272559/150‑5 (MQ=255)

                aaGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGCACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCg                                                                                                                                       <  2:7651653/150‑1 (MQ=255)

                  ggATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGgc                                                                                                                                     <  1:2362247/150‑1 (MQ=255)

                    aTGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGgcgc                                                                                                                                   <  1:819860/150‑1 (MQ=255)

                       cTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCa                                                                                                                                >  1:2169913/1‑150 (MQ=255)

                        tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCaa                                                                                                                               >  2:6594130/1‑150 (MQ=255)

                        tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCaa                                                                                                                               >  2:178169/1‑150 (MQ=255)

                        tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCaa                                                                                                                               >  2:2748779/1‑150 (MQ=255)

                          aCGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAAtt                                                                                                                             >  2:8334529/1‑150 (MQ=255)

                          aCGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAAtt                                                                                                                             <  1:456327/150‑1 (MQ=255)

                                tCACCAGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCa                                                                                                                       >  2:5275673/1‑150 (MQ=255)

                                    cTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCt                                                                                                                   >  2:8571571/1‑150 (MQ=255)

                                     tGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTg                                                                                                                  >  2:2726031/1‑150 (MQ=255)

                                          ccAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGt                                                                                                             >  2:1117317/1‑150 (MQ=255)

                                          ccAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGt                                                                                                             >  2:5703052/1‑150 (MQ=255)

                                           cAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTg                                                                                                            <  1:6653900/150‑1 (MQ=255)

                                            aGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                           <  2:1399200/150‑1 (MQ=255)

                                            aGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                           <  1:5910009/150‑1 (MQ=255)

                                              cTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACg                                                                                                         >  2:810447/1‑150 (MQ=255)

                                               tGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGa                                                                                                        >  1:6444352/1‑150 (MQ=255)

                                                 cAGGTCGAACTGCGCCAGCACCTGCTGCCACCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATc                                                                                                      <  1:7122329/150‑1 (MQ=255)

                                                 cAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATc                                                                                                      >  2:12358/1‑150 (MQ=255)

                                                  aGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCg                                                                                                     <  2:7055424/150‑1 (MQ=255)

                                                    gTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGc                                                                                                   <  1:78339/150‑1 (MQ=255)

                                                    gTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGc                                                                                                   >  1:7554217/1‑150 (MQ=255)

                                                    gTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGc                                                                                                   >  1:6903240/1‑150 (MQ=255)

                                                       gAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACg                                                                                                >  1:3671252/1‑150 (MQ=255)

                                                            ttggcagcagGTGCTGGCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGc                                                                                           >  2:6404823/5‑150 (MQ=255)

                                                             cgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGcc                                                                                          >  1:1002446/1‑150 (MQ=255)

                                                             cgcCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGcc                                                                                          >  1:1753080/1‑150 (MQ=255)

                                                               ccAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGa                                                                                        <  2:3140941/150‑1 (MQ=255)

                                                               ccAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGa                                                                                        <  1:5853312/150‑1 (MQ=255)

                                                                          tgcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAgg                                                                                                                     <  1:651747/110‑1 (MQ=255)

                                                                          tgcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATc                                                                             <  1:4312009/150‑1 (MQ=255)

                                                                             cAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                          <  1:3775548/150‑1 (MQ=255)

                                                                              aaCCACCCTCACGCGGGTAGCCCAAGCGGATGTCACTGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcg                                                                         <  1:7307815/150‑1 (MQ=255)

                                                                                  accCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCGATGGCgggag                                                                     <  2:1489263/150‑3 (MQ=255)

                                                                                   cccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaa                                                                    >  1:4673608/1‑150 (MQ=255)

                                                                                    ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                   <  1:8143350/150‑1 (MQ=255)

                                                                                    ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                   >  2:7364423/1‑150 (MQ=255)

                                                                                    ccTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCaaa                                                                   >  1:908062/1‑150 (MQ=255)

                                                                                     cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATc                                                                                                      >  1:6984226/1‑114 (MQ=255)

                                                                                     cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCGATGGCgggagtcg                                                                  <  2:2504764/150‑6 (MQ=255)

                                                                                     cTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAAc                                                                  >  1:5081179/1‑150 (MQ=255)

                                                                                        aCGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCt                                                               >  2:3028530/1‑150 (MQ=255)

                                                                                             ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGcc                                                          >  1:2741791/1‑150 (MQ=255)

                                                                                             ggTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGcc                                                          <  2:8959721/150‑1 (MQ=255)

                                                                                                   ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                                    >  1:3352169/1‑150 (MQ=255)

                                                                                                   ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                                    >  2:5890452/1‑150 (MQ=255)

                                                                                                   ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                                    >  1:8345441/1‑150 (MQ=255)

                                                                                                    cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                   <  2:8468256/150‑1 (MQ=255)

                                                                                                    cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                   >  2:6065630/1‑150 (MQ=255)

                                                                                                    cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                   >  2:400759/1‑150 (MQ=255)

                                                                                                    cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                   >  2:2375420/1‑150 (MQ=255)

                                                                                                    cAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCg                                                   >  2:3479975/1‑150 (MQ=255)

                                                                                                    cAAGCGGAAGACACGGGCCTCCAGCAACACCGCGCTCAAGCAACGCCTGCCTGGCCTTGCAGTTCGGCGCGCAATACCACCAGGCTGGGCGAGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCATCGCCgaaaacg                                                   >  1:1957931/1‑144 (MQ=255)

                                                                                                        cGGATGTCACGGGCCTCCAGCAACACCGCGCTCATCCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCaga                                               <  2:7901341/150‑1 (MQ=255)

                                                                                                         ggATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGTAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCagag                                              >  2:6228583/1‑150 (MQ=255)

                                                                                                         ggATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCagag                                              <  1:1143736/150‑1 (MQ=255)

                                                                                                          gATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAgg                                             <  2:2568212/150‑1 (MQ=255)

                                                                                                          gATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAgg                                             >  1:6183184/1‑150 (MQ=255)

                                                                                                               cACGGGCCTCCAGCACCACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGc                                        <  1:5581819/150‑1 (MQ=255)

                                                                                                                aCGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGcc                                       >  2:5008764/1‑150 (MQ=255)

                                                                                                                  gggCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCCAACCCTTCGCCGTATTCGCAGAGGTAAGCCtt                                     >  2:325072/1‑150 (MQ=255)

                                                                                                                   ggCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTg                                    <  1:2748779/150‑1 (MQ=255)

                                                                                                                           gCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACt                            >  2:2880750/1‑150 (MQ=255)

                                                                                                                             aaCACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTgg                          <  1:4524844/150‑1 (MQ=255)

                                                                                                                               cacCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGcc                        <  2:4834063/150‑1 (MQ=255)

                                                                                                                                  cgcgCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGc                     >  1:8628501/1‑150 (MQ=255)

                                                                                                                                     gcTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAAGTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAAc                  <  2:3300567/150‑1 (MQ=255)

                                                                                                                                        cATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTcc               <  2:4723045/150‑1 (MQ=255)

                                                                                                                                         ctGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa              >  1:5146909/2‑150 (MQ=255)

                                                                                                                                         aTGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGATGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa              <  1:4787438/150‑1 (MQ=255)

                                                                                                                                         aTGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa              >  1:1566781/1‑150 (MQ=255)

                                                                                                                                         aTGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa              <  1:938842/150‑1 (MQ=255)

                                                                                                                                         aTGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa              >  2:1632964/1‑150 (MQ=255)

                                                                                                                                          ttgcgcGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg             >  2:8754084/6‑150 (MQ=255)

                                                                                                                                          ttgcgcGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg             >  2:4148665/6‑150 (MQ=255)

                                                                                                                                          tGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg             >  1:3965319/1‑150 (MQ=255)

                                                                                                                                          tGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg             >  1:4987210/1‑150 (MQ=255)

                                                                                                                                          tGCAACGCCAGGCAGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg             >  1:2960351/1‑150 (MQ=255)

                                                                                                                                            cAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCATAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt           <  1:8459186/150‑1 (MQ=255)

                                                                                                                                            cAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt           >  2:3850298/1‑150 (MQ=255)

                                                                                                                                             aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt           <  2:6747341/149‑1 (MQ=255)

                                                                                                                                             aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt           >  1:6747341/1‑149 (MQ=255)

                                                                                                                                             aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTg          >  1:3216879/1‑150 (MQ=255)

                                                                                                                                             aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTg          >  1:1828422/1‑150 (MQ=255)

                                                                                                                                               cGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTGGAGTTgca        <  1:8890770/150‑3 (MQ=255)

                                                                                                                                                gcctgcctGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCt                       <  2:3606299/134‑1 (MQ=255)

                                                                                                                                                 cctgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTAcc      <  1:5275673/150‑1 (MQ=255)

                                                                                                                                                   tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcgg                                                                        >  2:4252824/1‑82 (MQ=255)

                                                                                                                                                   tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACcgc    <  2:2341513/150‑1 (MQ=255)

                                                                                                                                                     cctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGCGt  <  2:663334/150‑1 (MQ=255)

CCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGCGT  >  NC_002947/4135796‑4136094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`