BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,250,320	C→T	5.3%	R558H (CGC→CAC)	PP_3724 ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,250,320	0	C	T	5.3%	962.4 / 26.5	300	R558H (CGC→CAC)	PP_3724	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (157/127); new base T (9/7); total (166/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

GCACGCCGCCGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTC  >  NC_002947/4250171‑4250468

gCACGCCGCCGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGgc                                                                                                                                                      <  1:8475130/150‑1 (MQ=255)

    gccgccGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCAt                                                                                                                                                  >  2:4367137/1‑150 (MQ=255)

     ccgccgGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATc                                                                                                                                                 >  2:5829774/1‑150 (MQ=255)

       gccgGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTg                                                                                                                                               <  1:4602343/150‑1 (MQ=255)

       gccgGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTg                                                                                                                                               >  1:3817180/1‑150 (MQ=255)

           gCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCAcc                                                                                                                                           >  2:8277720/1‑150 (MQ=255)

              ccGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGTGCAGATGCGCCAGt                                                                                                                                        <  1:7173148/150‑1 (MQ=255)

              ccGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGt                                                                                                                                        <  1:1574300/150‑1 (MQ=255)

               cGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAGCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTg                                                                                                                                       >  1:6708916/1‑150 (MQ=255)

               cGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAATCTGCGGGGCGTAGTCAGCGATGCACTGGTGCAGATGCGCCAGTg                                                                                                                                       <  2:5953699/150‑1 (MQ=255)

               cGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTg                                                                                                                                       <  1:2664990/150‑1 (MQ=255)

                gATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGc                                                                                                                                      <  2:7749572/150‑1 (MQ=255)

                   cACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGc                                                                                                                                   <  2:7462211/150‑1 (MQ=255)

                    aCCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCt                                                                                                                                  >  1:6524870/1‑150 (MQ=255)

                     ccAAGGCCAGGGCACAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCtg                                                                                                                                 >  2:1200734/1‑149 (MQ=255)

                     ccAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCtt                                                                                                                                 >  2:6482425/1‑150 (MQ=255)

                     ccAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCtt                                                                                                                                 >  1:6267866/1‑150 (MQ=255)

                      cAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTc                                                                                                                                >  1:4193600/1‑150 (MQ=255)

                       aaGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTcc                                                                                                                               >  2:5310600/1‑150 (MQ=255)

                       aaGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTcc                                                                                                                               >  2:4753680/1‑150 (MQ=255)

                       aaGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTcc                                                                                                                               >  2:738390/1‑150 (MQ=255)

                         ggCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAg                                                                                                                             <  2:3425284/150‑1 (MQ=255)

                          gCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAgg                                                                                                                            >  1:3348554/1‑150 (MQ=255)

                               ggCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGcgcg                                                                                                                       <  2:4274617/150‑1 (MQ=255)

                                gCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCgg                                                                                                                      <  2:6607579/150‑1 (MQ=255)

                                 cAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCggg                                                                                                                     <  2:3647134/150‑1 (MQ=255)

                                  aaaGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGc                                                                                                                    >  2:8072399/1‑150 (MQ=255)

                                       tgttTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATcgc                                                                                                               <  1:7202712/150‑1 (MQ=255)

                                         ttttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCgcgc                                                                                                             >  2:5611463/1‑150 (MQ=255)

                                         ttttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCgcgc                                                                                                             <  1:1693390/150‑1 (MQ=255)

                                          tttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCa                                                                                                            <  1:1265934/150‑1 (MQ=255)

                                           ttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                           <  2:833733/150‑1 (MQ=255)

                                              aCGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGtt                                                                                                        <  1:7271146/150‑1 (MQ=255)

                                                  ttGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCCGCGATGCACTGGCGCATCTGCACCAGTGCAGCGTCCAGGGCGCGGGCCTCGCGCCGGTTCAAc                                                                                                    >  1:8551648/1‑150 (MQ=255)

                                                  ttGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAAc                                                                                                    <  2:4864710/150‑1 (MQ=255)

                                                  ttGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAAc                                                                                                    <  1:8750397/150‑1 (MQ=255)

                                                  ttGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAAc                                                                                                    <  1:6734412/150‑1 (MQ=255)

                                                    gATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGc                                                                                                  <  2:7845868/150‑1 (MQ=255)

                                                        cccACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCcaca                                                                                              <  2:3870906/150‑1 (MQ=255)

                                                          cACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCCTCGCGCAGGTTCAACGCCACAGc                                                                                            >  2:6824382/1‑150 (MQ=255)

                                                          cACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGc                                                                                            >  1:5249081/1‑150 (MQ=255)

                                                          cACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGc                                                                                            >  2:1054227/1‑150 (MQ=255)

                                                           aCTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACACAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCa                                                                                           >  1:7580549/1‑150 (MQ=255)

                                                            cTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCAc                                                                                          >  2:7101704/1‑150 (MQ=255)

                                                             tATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCAcc                                                                                         >  2:5469105/1‑150 (MQ=255)

                                                              aTGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCa                                                                                        <  1:7756118/150‑1 (MQ=255)

                                                                gAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGc                                                                                      >  1:620477/1‑150 (MQ=255)

                                                                  gCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTACACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCtt                                                                                    >  2:8424548/1‑150 (MQ=255)

                                                                   cTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTc                                                                                   <  2:3117183/150‑1 (MQ=255)

                                                                    tCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCg                                                                                  >  2:4031704/1‑150 (MQ=255)

                                                                      ggCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGt                                                                                <  1:2039654/150‑1 (MQ=255)

                                                                          aaGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtg                                                                            <  2:2274828/150‑1 (MQ=255)

                                                                           aGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtgt                                                                           >  1:5577869/1‑150 (MQ=255)

                                                                            gggCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtgtg                                                                          >  2:899941/1‑150 (MQ=255)

                                                                             ggcggcTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTACAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGGCGTGTGc                                                                         >  1:1758697/1‑150 (MQ=255)

                                                                                     ggCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTg                                                                 >  2:1901523/1‑150 (MQ=255)

                                                                                      gCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCaa                                                                                                     >  1:3689847/1‑113 (MQ=255)

                                                                                        cATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgc                                                              <  1:6416189/150‑1 (MQ=255)

                                                                                             gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                         >  2:537967/1‑150 (MQ=255)

                                                                                             gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                         >  2:6436060/1‑150 (MQ=255)

                                                                                             gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                         >  1:4380141/1‑150 (MQ=255)

                                                                                             gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                         >  2:8871208/1‑150 (MQ=255)

                                                                                             gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                         >  1:6796628/1‑150 (MQ=255)

                                                                                             gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                         <  2:1639393/150‑1 (MQ=255)

                                                                                              cTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAc                                                        >  2:6349497/1‑150 (MQ=255)

                                                                                               tCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAcc                                                       >  1:7222273/1‑150 (MQ=255)

                                                                                               tCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAcc                                                       <  1:1609484/150‑1 (MQ=255)

                                                                                                 gAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGc                                                     <  1:7460572/150‑1 (MQ=255)

                                                                                                    cagcaCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCtt                                                  >  2:7369547/1‑150 (MQ=255)

                                                                                                      ctaCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCa                                                <  2:3982403/148‑1 (MQ=255)

                                                                                                       caCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTcgg                                               >  1:5481711/1‑148 (MQ=255)

                                                                                                       caCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAg                                               >  1:1004373/1‑150 (MQ=255)

                                                                                                                aaaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAggg                                      <  2:2404175/150‑1 (MQ=255)

                                                                                                                 aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAgggg                                     >  1:5893480/1‑150 (MQ=255)

                                                                                                                 aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAgggg                                     >  1:5353782/1‑150 (MQ=255)

                                                                                                                  aCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGt                                    <  2:3892266/150‑1 (MQ=255)

                                                                                                                  aCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGt                                    >  2:5740785/1‑150 (MQ=255)

                                                                                                                      aaCCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAAcc                                >  1:4728379/1‑150 (MQ=255)

                                                                                                                        ccTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGa                              >  2:2882147/1‑150 (MQ=255)

                                                                                                                         cTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAggg                                      >  2:6089465/1‑141 (MQ=255)

                                                                                                                         cTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGAc                             >  2:4621608/1‑150 (MQ=255)

                                                                                                                               ggCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAACTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACgcgggc                       >  1:6938046/1‑150 (MQ=255)

                                                                                                                                  gTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGggcggc                    <  2:412983/150‑1 (MQ=255)

                                                                                                                                  gTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGggcggc                    <  2:3348570/150‑1 (MQ=255)

                                                                                                                                  gTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGggcggc                    <  1:8132412/150‑1 (MQ=255)

                                                                                                                                  gTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGggcggc                    >  2:920981/1‑150 (MQ=255)

                                                                                                                                     gTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTc                 >  1:2527915/1‑150 (MQ=255)

                                                                                                                                     gTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTc                 >  1:9068257/1‑150 (MQ=255)

                                                                                                                                      tCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt                >  1:8679280/1‑150 (MQ=255)

                                                                                                                                      acAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt                <  2:153076/149‑1 (MQ=255)

                                                                                                                                        aGCGATGCGCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGc              >  1:6010778/1‑150 (MQ=255)

                                                                                                                                         aagctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGcc             >  2:8616834/5‑150 (MQ=255)

                                                                                                                                               cACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGgcg       >  2:2747862/1‑150 (MQ=255)

                                                                                                                                                aCTGGTGCAGATGCGCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGgcgc      >  2:5057244/1‑150 (MQ=255)

                                                                                                                                                aCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCgg                                                                       >  2:1135603/1‑85 (MQ=255)

                                                                                                                                                aCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCgg                                                                       <  1:1135603/85‑1 (MQ=255)

                                                                                                                                                aCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGgcgc      <  1:1200734/150‑1 (MQ=255)

                                                                                                                                                 cTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGcgcg     >  1:495462/1‑150 (MQ=255)

                                                                                                                                                    gcgcATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCACGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTc  >  1:3054365/1‑150 (MQ=255)

                                                                                                                                                    gcgcATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTc  <  2:7199687/150‑1 (MQ=255)

GCACGCCGCCGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTC  >  NC_002947/4250171‑4250468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`