Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,552,849 G→C 5.2% L84L (CTG→CTC)  pydA → NADP‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,552,8490GC 5.2% 1173.1 / 37.9 345L84L (CTG→CTC) pydANADP‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base G (161/166);  new base C (11/7);  total (172/173)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 355 total aligned reads displayed.

TTCGAGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGAA  >  NC_002947/4552700‑4552998
                                                                                                                                                     |                                                                                                                                                     
ttCGAGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTg                                                                                                                                                       >  2:8954738/1‑150 (MQ=255)
ttCGAGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTg                                                                                                                                                       <  1:4989900/150‑1 (MQ=255)
 tCGAGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTgg                                                                                                                                                      >  1:385058/1‑150 (MQ=255)
  cGAGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGa                                                                                                                                                     >  2:2143135/1‑150 (MQ=255)
   gAGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGaa                                                                                                                                                    >  1:4467161/1‑150 (MQ=255)
    aGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGaaa                                                                                                                                                   <  1:5176568/150‑1 (MQ=255)
      gCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATc                                                                                                                                                 >  1:9026684/1‑150 (MQ=255)
       ncGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCa                                                                                                                                                >  1:6933452/2‑150 (MQ=255)
         ggCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAAc                                                                                                                                              >  1:757702/1‑150 (MQ=255)
          gCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAAcc                                                                                                                                             >  2:3922713/1‑150 (MQ=255)
             ggggCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGc                                                                                                                                          <  2:6217597/150‑1 (MQ=255)
                   gcgTGGTCTGGAAGACCCTGCGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACACGGGATCAACAATATCGAGTTCATCACCGACCGCTCTCTGGAAATCAACCTGCGTGaaa                                                                                                                                    <  2:1346913/150‑1 (MQ=255)
                           tGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAg                                                                                                                            >  2:8382782/1‑150 (MQ=255)
                           tGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAg                                                                                                                            <  1:2003670/150‑1 (MQ=255)
                                gACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTgaa                                                                                                                       <  1:2199992/150‑1 (MQ=255)
                                 aCCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTgaag                                                                                                                      <  1:6346639/150‑1 (MQ=255)
                                 aCCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTgaag                                                                                                                      <  1:8763493/150‑1 (MQ=255)
                                   ccTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTgaagaa                                                                                                                    >  2:4947510/1‑150 (MQ=255)
                                   ccTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTgaagaa                                                                                                                    <  1:8089943/150‑1 (MQ=255)
                                   ccTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTgaagaa                                                                                                                    >  2:8943143/1‑150 (MQ=255)
                                   ccTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTgaagaa                                                                                                                    >  1:1612264/1‑150 (MQ=255)
                                     tGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAgg                                                                                                                  <  1:1481600/150‑1 (MQ=255)
                                     tGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAgg                                                                                                                  <  1:5683213/150‑1 (MQ=255)
                                     tGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAgg                                                                                                                  <  1:5986319/150‑1 (MQ=255)
                                           aaGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGc                                                                                                            <  1:1108815/150‑1 (MQ=255)
                                                ccTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGAc                                                                                                       >  2:4384448/1‑150 (MQ=255)
                                                ccTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGAc                                                                                                       >  2:4269452/1‑150 (MQ=255)
                                                ccTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGAc                                                                                                       >  1:5798945/1‑150 (MQ=255)
                                                ccTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGAc                                                                                                       >  2:8785144/1‑150 (MQ=255)
                                                    cggcggTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACcgcg                                                                                                   <  2:6085879/150‑1 (MQ=255)
                                                      gcggTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCa                                                                                                 >  2:2987655/1‑150 (MQ=255)
                                                            aaCGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATc                                                                                           >  2:5508318/1‑150 (MQ=255)
                                                             aCGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCa                                                                                                                             >  1:6071458/1‑115 (MQ=255)
                                                              cGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCgt                                                                                         <  1:3090467/150‑1 (MQ=255)
                                                                tgtCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCgtgt                                                                                       <  2:7932982/150‑1 (MQ=255)
                                                                  tcgtcgCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACACTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGACGAAGGACTGGCCCGACCGCGCACTGATCGTGTCg                                                                                     >  2:8221007/1‑150 (MQ=255)
                                                                     tcgCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAg                                                                                                                            <  1:8975174/108‑1 (MQ=255)
                                                                     tcgCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGACGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTg                                                                                  >  1:2724987/1‑150 (MQ=255)
                                                                     tcgCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTg                                                                                  >  2:7732821/1‑150 (MQ=255)
                                                                      cgcgCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGa                                                                                 <  2:7056165/150‑1 (MQ=255)
                                                                      cgcgCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGa                                                                                 <  2:7275089/150‑1 (MQ=255)
                                                                       gcgcTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGAt                                                                                <  2:744778/150‑1 (MQ=255)
                                                                        cgcTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATg                                                                               >  2:9166795/1‑150 (MQ=255)
                                                                         gcTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATgg                                                                              >  1:2542901/1‑150 (MQ=255)
                                                                           tACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTg                                                                            >  2:239943/1‑150 (MQ=255)
                                                                           tACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTg                                                                            >  1:7049261/1‑150 (MQ=255)
                                                                              tCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCg                                                                         >  1:4056339/1‑150 (MQ=255)
                                                                              tCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCg                                                                         >  2:3127002/1‑150 (MQ=255)
                                                                               cGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGt                                                                        <  1:825689/150‑1 (MQ=255)
                                                                               cGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGt                                                                        >  1:347363/1‑150 (MQ=255)
                                                                                ggCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTg                                                                       <  2:6714290/150‑1 (MQ=255)
                                                                                 gcgcACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGc                                                                      >  1:7295705/1‑150 (MQ=255)
                                                                                   gcACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCAt                                                                    >  2:3602627/1‑150 (MQ=255)
                                                                                       tATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATagag                                                                <  1:3642018/150‑1 (MQ=255)
                                                                                          ggCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGaa                                                             >  2:4775916/1‑150 (MQ=255)
                                                                                             cccAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCg                                                          >  1:5431821/1‑150 (MQ=255)
                                                                                                   cGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAg                                                    <  1:5268069/150‑1 (MQ=255)
                                                                                                             aGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGc                                          <  2:1414642/150‑1 (MQ=255)
                                                                                                             aGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGc                                          <  1:473328/150‑1 (MQ=255)
                                                                                                              ggggATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGcc                                         <  1:3322305/150‑1 (MQ=255)
                                                                                                               gggATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCg                                        >  2:1982405/1‑150 (MQ=255)
                                                                                                                 tctCAACAATATCGAGCTGATCACCGACCGGCCGCTCGAAGTGAACCTGCGCGAAATGAAAGAAGTGAAAATGCGCTGGCCCGACCGCGCACTGATCGCTTCGATCATGGTGCCATGCGAAGAAGAAGCGTGGAAAGCAATCCTGCCCCt                                      >  1:1216181/3‑150 (MQ=255)
                                                                                                                   tCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtgg                                    <  1:6752975/150‑1 (MQ=255)
                                                                                                                   tCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtgg                                    <  2:5977884/150‑1 (MQ=255)
                                                                                                                   tCAACAATATCGAGGTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGACTCGTGGAAGTTCATCCTGCCGCggg                                    >  2:8592108/1‑150 (MQ=255)
                                                                                                                   tCAACAATATCGAGCTGATCACCGACCGGCCGCTCGAAGTGAACCTGCGCGAAATGAAAGAAGTGAAAATGCGCTGGCCCGACCGCGCACTGATCGCTTCGATCATGGTGCCATGCGAAGAAGAAGCGTGGAAAGCAATCCTGCCCCtgg                                    >  1:1014614/1‑150 (MQ=255)
                                                                                                                     aacaaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtggtg                                  >  1:4307217/1‑150 (MQ=255)
                                                                                                                     aacaaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtggtg                                  >  1:6497224/1‑150 (MQ=255)
                                                                                                                     aacaaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtggtg                                  >  1:3018839/1‑150 (MQ=255)
                                                                                                                     aacaaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtggtg                                  >  1:7442058/1‑150 (MQ=255)
                                                                                                                     aacaaTATCGAGCTGATCACCGACCGGCCGCTCGAAGTGAACCTGCGCGAAATGAAAGAAGTGAAAATGCGCTGGCCCGACCGCGCACTGATCGCTTCGATCATGGTGCCATGCGAAGAAGAAGCGTGGAAAGCAATCCTGCCCCtggtg                                  <  2:4651042/150‑1 (MQ=255)
                                                                                                                      acaaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtggtgg                                 >  2:8176082/1‑150 (MQ=255)
                                                                                                                      acaaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCtggtgg                                 >  2:1130512/1‑150 (MQ=255)
                                                                                                                       caaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGa                                <  1:2143135/150‑1 (MQ=255)
                                                                                                                        aaTATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGaa                               <  2:2737993/150‑1 (MQ=255)
                                                                                                                        aaTATCGAGCTGATCACCGACCGGCCGCTCGAAGTGAACCTGCGCGAAATGAAAGAAGTGAAAATGCGCTGGCCCGACCGCGCACTGATCGCTTCGATCATGGTGCCATGCGAAGAAGAAGCGTGGAAAGCAATCCTGCCCCTGGTGGaa                               <  2:6494681/150‑1 (MQ=255)
                                                                                                                            tCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCa                           >  1:6197804/1‑150 (MQ=255)
                                                                                                                            tCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCa                           >  2:1251842/1‑150 (MQ=255)
                                                                                                                             cGAGCTGCTCACCGACCGGCCGCTCGAAGTGAACCTGTGCGAAATGAAAGAAGTGAAAATGCGCTGGCCCGACCGCGCACTGATCGCTTCGATCATGGTGCCATGCGAAGAAGAAGCGTGGAAAGCAATCCTGCCCCTGGTGGAAgaaac                          <  2:5800464/150‑5 (MQ=255)
                                                                                                                              ttcacgATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGCTGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACg                         >  2:7362828/6‑150 (MQ=255)
                                                                                                                              gAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACg                         >  2:4064527/1‑150 (MQ=255)
                                                                                                                               aGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACgg                        >  1:6588505/1‑150 (MQ=255)
                                                                                                                               aGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACgg                        <  2:1855205/150‑1 (MQ=255)
                                                                                                                                    aTCACCGACCGGCCGCTCGAAGTGAACCTGCGCGAAATGAAAGAAGTGAAAATGCGCTGGCCCGACCGCGCACTGATCGCTTCGATCATGGTGccat                                                                        >  2:5158669/1‑95 (MQ=255)
                                                                                                                                     tCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCg                  >  1:5538179/1‑150 (MQ=255)
                                                                                                                                        ccgaccgCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATg               <  1:7907219/150‑1 (MQ=255)
                                                                                                                                        ccgaccgCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATg               <  2:2678982/150‑1 (MQ=255)
                                                                                                                                         cgaccgCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATgg              <  1:1284918/150‑1 (MQ=255)
                                                                                                                                         cgaccgCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATgg              <  2:8549144/150‑1 (MQ=255)
                                                                                                                                          gaccgCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGc             <  1:956401/150‑1 (MQ=255)
                                                                                                                                                 ctctGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGc      <  2:1599093/150‑1 (MQ=255)
                                                                                                                                                 ctctGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGc      <  2:2902688/150‑1 (MQ=255)
                                                                                                                                                 ctctGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGc      <  2:5189951/150‑1 (MQ=255)
                                                                                                                                                    tGGAAATCAACCTGCGTTAAATCACCCAGTTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGa   <  1:8553328/150‑1 (MQ=255)
                                                                                                                                                    tGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGa   <  1:1684819/150‑1 (MQ=255)
                                                                                                                                                    tGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGa   >  1:7772290/1‑150 (MQ=255)
                                                                                                                                                    tGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGa   >  1:2387830/1‑150 (MQ=255)
                                                                                                                                                    tGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGa   >  2:7585703/1‑150 (MQ=255)
                                                                                                                                                    tGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGa   >  2:3443283/1‑150 (MQ=255)
                                                                                                                                                    tGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGa   >  2:4715959/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCGAGGCCGGCTGGGGCGGCGTGGTCTGGAAGACCCTGGGCGAAGACCCTGCGGCGGTCAACGTGTCGTCGCGCTACTCGGCGCACTATGGCCCCAACCGTCAGGTACAGGGGATCAACAATATCGAGTTGATCACCGACCGCTCTCTGGAAATCAACCTGCGTGAAATCACCCAGGTGAAGAAGGACTGGCCCGACCGCGCACTGATCGTGTCGCTGATGGTGCCGTGCATAGAGGAATCGTGGAAGTTCATCCTGCCGCTGGTGGAAGCCACGGGGGCCGATGGCATCGAGCTGAA  >  NC_002947/4552700‑4552998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑