Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,748,229 C→T 21.4% A250A (GCG→GCA)  etfA ← electron transfer flavoprotein subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,748,2290CT21.4% 655.0 / 175.7 322A250A (GCG→GCA) etfAelectron transfer flavoprotein subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (136/117);  new base T (35/34);  total (171/151)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

GAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATGCCACGGC  >  NC_002947/4748082‑4748377
                                                                                                                                                   |                                                                                                                                                    
  aCAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCaa                                                                                                                                                  >  2:6698644/1‑150 (MQ=255)
    agatCACCGACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAAcc                                                                                                                                                >  1:3326451/4‑149 (MQ=255)
         gccgacgaGGCCATAATCAGCCACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCtt                                                                                                                                           >  2:8183116/3‑150 (MQ=255)
         accgacgaGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCtt                                                                                                                                           >  1:8543493/3‑150 (MQ=255)
         aGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCtt                                                                                                                                           >  1:1699617/1‑150 (MQ=255)
          gCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTg                                                                                                                                          >  2:839595/1‑150 (MQ=255)
          ccgacgaGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTg                                                                                                                                          >  1:863492/2‑150 (MQ=255)
             acgaGGCCATAATCAGCCACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCg                                                                                                                                       <  1:7370423/147‑1 (MQ=255)
             acgaGGCCATAATCAGCCACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCg                                                                                                                                       >  2:5391288/4‑150 (MQ=255)
             acgaGGCCATAATCAGCCACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCg                                                                                                                                       <  1:7206930/147‑1 (MQ=255)
             aCCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCg                                                                                                                                       <  1:1036080/150‑1 (MQ=255)
               gaGGCCATAATCAGCCACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGt                                                                                                                                     <  2:3871653/149‑1 (MQ=255)
               cAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGt                                                                                                                                     >  1:1566970/1‑150 (MQ=255)
               cAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGt                                                                                                                                     >  2:5745131/1‑150 (MQ=255)
                 ggCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCt                                                                                                                                   <  1:1276140/150‑1 (MQ=255)
                   ccataatCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTgg                                                                                                                                 <  1:8443915/144‑1 (MQ=255)
                   ccGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTgg                                                                                                                                 <  1:6929686/150‑1 (MQ=255)
                    cataatCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGc                                                                                                                                >  1:6090122/6‑150 (MQ=255)
                    cGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCAGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGc                                                                                                                                <  1:2936992/150‑1 (MQ=255)
                     ataatcagCCACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGcc                                                                                                                               >  2:6558171/8‑150 (MQ=255)
                       aGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGa                                                                                                                             >  1:5067761/1‑150 (MQ=255)
                        atCGGCAACCTGGAAGATCGGCGCTTCCTCATCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAAc                                                                                                                            >  2:1832906/2‑150 (MQ=255)
                         tCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGAcc                                                                                                                           >  2:4719046/1‑150 (MQ=255)
                          cagCCACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAACCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCt                                                                                                                          >  2:1243467/3‑150 (MQ=255)
                          cGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCt                                                                                                                          <  1:4889152/150‑1 (MQ=255)
                          cGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCt                                                                                                                          <  2:6036452/150‑1 (MQ=255)
                            gcaaCCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGc                                                                                                                        <  2:7322944/147‑1 (MQ=255)
                            gcaaCCTGGAAGATCGGCGCTTCCTCATCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGc                                                                                                                        <  2:4812455/147‑1 (MQ=255)
                            gCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGc                                                                                                                        >  1:3603177/1‑150 (MQ=255)
                             ccACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGcc                                                                                                                       >  2:5580956/1‑149 (MQ=255)
                             ccACCTGGAAGATCGGTGCTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGcc                                                                                                                       >  1:2357461/1‑149 (MQ=255)
                              aaCCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGcca                                                                                                                      <  1:4544532/149‑3 (MQ=255)
                              aaCCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGcca                                                                                                                      <  2:7691485/149‑3 (MQ=255)
                               aCCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATg                                                                                                                     >  2:3199790/1‑150 (MQ=255)
                                    gAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGtt                                                                                                                >  2:8918206/1‑150 (MQ=255)
                                     aaGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTc                                                                                                               <  1:13820/150‑1 (MQ=255)
                                     aaGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTc                                                                                                               <  1:8986134/150‑1 (MQ=255)
                                       gATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTTGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCgg                                                                                                             >  2:5873802/1‑150 (MQ=255)
                                        aTCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGc                                                                                                            >  1:5999831/1‑150 (MQ=255)
                                        aTCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGc                                                                                                            <  2:3645109/150‑1 (MQ=255)
                                         tCGGCGCTTCTTCGTCCTTGCTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCAt                                                                                                                      >  2:3315855/1‑139 (MQ=255)
                                          cGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGCCAGTCGTTCGGcgc                                                                                                          >  1:8715553/1‑148 (MQ=255)
                                            gtgcTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGCCAGTCGTTTGGcgcat                                                                                                        >  1:4029313/3‑146 (MQ=255)
                                            gcgcTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGCCAGTCGTTCGGcgcat                                                                                                        >  1:6540279/1‑146 (MQ=255)
                                            gcgcTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGCCAGTCGTTCGGcgcat                                                                                                        >  2:4878719/1‑146 (MQ=255)
                                            gcgcTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGCCAGTCGTTCGGcgcat                                                                                                        >  2:2355597/1‑146 (MQ=255)
                                             cgcTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGaa                                                                                                       <  2:8607773/150‑1 (MQ=255)
                                             cgcTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGaa                                                                                                       <  2:7119170/150‑1 (MQ=255)
                                               cttcttCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGc                                                                                                     <  1:5866893/150‑1 (MQ=255)
                                                      gTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTc                                                                                              <  1:2792967/150‑1 (MQ=255)
                                                      gTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTc                                                                                              >  2:257906/1‑150 (MQ=255)
                                                        ccTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGa                                                                                            <  1:3845042/150‑1 (MQ=255)
                                                           tgttgATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTg                                                                                         <  1:1331567/150‑1 (MQ=255)
                                                             ttgATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTgcg                                                                                       >  1:497762/1‑150 (MQ=255)
                                                               gATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTgcggc                                                                                     <  2:5001057/150‑1 (MQ=255)
                                                                   gCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGgcgcgc                                                                                 <  2:6161922/150‑1 (MQ=255)
                                                                    ccACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGcgcgcg                                                                                >  1:1994754/1‑150 (MQ=255)
                                                                      aCGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGaa                                                                              <  1:532705/150‑1 (MQ=255)
                                                                        gATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGc                                                                            >  1:1017471/1‑150 (MQ=255)
                                                                          tCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCAc                                                                          >  1:3491919/1‑150 (MQ=255)
                                                                             cTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCaa                                                                       <  1:5546621/150‑1 (MQ=255)
                                                                              tttGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAAc                                                                      >  2:251490/1‑150 (MQ=255)
                                                                              tttGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAAc                                                                      >  1:5153705/1‑150 (MQ=255)
                                                                              tttGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAAc                                                                      <  1:7464041/150‑1 (MQ=255)
                                                                              tttGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAAc                                                                      >  1:424208/1‑150 (MQ=255)
                                                                              tttGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAAc                                                                      >  2:4668695/1‑150 (MQ=255)
                                                                              tttGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAAc                                                                      >  1:7066617/1‑150 (MQ=255)
                                                                                 ggAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGc                                                                   >  1:4677803/1‑150 (MQ=255)
                                                                                 ggAGTCTTTCATGCCGGCCAGGTGCTGGATCACGCCGGAGATACTGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGc                                                                   <  2:7538463/150‑1 (MQ=255)
                                                                                  gAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCa                                                                  <  1:257091/150‑1 (MQ=255)
                                                                                  gAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCa                                                                  >  2:4281217/1‑150 (MQ=255)
                                                                                   aGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAg                                                                 <  2:1184134/150‑1 (MQ=255)
                                                                                       tttCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGcc                                                             >  2:5809155/1‑150 (MQ=255)
                                                                                       tttCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGcc                                                             >  1:499622/1‑150 (MQ=255)
                                                                                       tttCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGACACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGcc                                                             >  1:7250495/1‑150 (MQ=255)
                                                                                            tGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCt                                                        >  2:1212300/1‑150 (MQ=255)
                                                                                             gCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCtt                                                       >  1:4176278/1‑150 (MQ=255)
                                                                                             gCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCtt                                                       <  2:1017471/150‑1 (MQ=255)
                                                                                              ccGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTg                                                      <  2:7412498/150‑1 (MQ=255)
                                                                                                    aGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGcc                                                >  2:5104104/1‑150 (MQ=255)
                                                                                                     ggTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCa                                               <  1:4485941/150‑1 (MQ=255)
                                                                                                      gTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAg                                              <  1:3086624/150‑1 (MQ=255)
                                                                                                          tGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTg                                          >  2:3388922/1‑150 (MQ=255)
                                                                                                            gATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCAAGGCTGTa                                        >  2:8565410/1‑150 (MQ=255)
                                                                                                                        gaTACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCGGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGaa                            <  1:6698749/150‑1 (MQ=255)
                                                                                                                        gaTACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGaa                            <  1:7590950/150‑1 (MQ=255)
                                                                                                                        gaTACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGaa                            <  2:8456829/150‑1 (MQ=255)
                                                                                                                        gaTACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGaa                            <  1:7853357/150‑1 (MQ=255)
                                                                                                                         aTACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAATCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAg                           >  2:2973963/1‑150 (MQ=255)
                                                                                                                           aCCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGtt                         <  2:2094452/150‑1 (MQ=255)
                                                                                                                             cGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGt                       >  1:6344942/1‑150 (MQ=255)
                                                                                                                             cGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGt                       >  1:2694472/1‑150 (MQ=255)
                                                                                                                                 ggCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCAcc                   >  2:5911768/1‑150 (MQ=255)
                                                                                                                                  gCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCg                  >  2:706714/1‑150 (MQ=255)
                                                                                                                                  gCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCg                  <  2:2783489/150‑1 (MQ=255)
                                                                                                                                           aGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATg         >  2:800805/1‑150 (MQ=255)
                                                                                                                                             cTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGTAGTTGTCACCGTTACCCATGcc       <  2:8801529/150‑1 (MQ=255)
                                                                                                                                              tgtgGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg                                                  <  2:1492099/106‑1 (MQ=255)
                                                                                                                                              tgtgGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg                                                  >  1:1492099/1‑106 (MQ=255)
                                                                                                                                              tgtgGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATGCCa      >  1:8016275/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATGCCACGGC  >  NC_002947/4748082‑4748377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑