BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,030,742	A→G	7.0%	N347S (AAC→AGC) ‡	PP_4432 →	Xaa‑Pro aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,030,742	0	A	G	7.0%	884.5 / 35.9	284	N347S (AAC→AGC) ‡	PP_4432	Xaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand): ref base A (119/145); new base G (12/8); total (131/153)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 291 total aligned reads displayed.

GGCAACACCACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGAC  >  NC_002947/5030594‑5030890

ggCAACACCACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGacaac                                                                                                                                                     <  2:1853125/150‑1 (MQ=255)

   aaCACCACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGc                                                                                                                                                  >  1:1092556/1‑150 (MQ=255)

      accacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTg                                                                                                                                               >  2:2944172/1‑150 (MQ=255)

       ccacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGc                                                                                                                                              <  2:5144996/150‑1 (MQ=255)

       ccacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGc                                                                                                                                              <  2:4513044/150‑1 (MQ=255)

        cacTGGTGATATCGCACGTGCCTTCTTCGGCGTGTTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCt                                                                                                                                             <  2:3645827/150‑1 (MQ=255)

        cacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCt                                                                                                                                             <  2:1794611/150‑1 (MQ=255)

        cacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCt                                                                                                                                             >  1:7543559/1‑150 (MQ=255)

         acTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTc                                                                                                                                            <  1:83016/150‑1 (MQ=255)

                atatCGCACGTGCCGTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTg                                                                                                                                     <  2:1365723/150‑1 (MQ=255)

                 tatCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTgg                                                                                                                                    <  2:2796158/150‑1 (MQ=255)

                 tatCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTgg                                                                                                                                    <  2:463326/150‑1 (MQ=255)

                  atCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGt                                                                                                                                   >  1:8410497/1‑150 (MQ=255)

                    cGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTAt                                                                                                                                 <  2:3477163/150‑1 (MQ=255)

                     gCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATg                                                                                                                                <  1:6970152/150‑1 (MQ=255)

                           gCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTc                                                                                                                          >  1:305988/1‑150 (MQ=255)

                           gCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTc                                                                                                                          <  1:3776326/150‑1 (MQ=255)

                             cttcttCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCa                                                                                                                        >  1:1719442/1‑150 (MQ=255)

                              ttcttcGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCAc                                                                                                                       >  1:9038856/1‑150 (MQ=255)

                              ttcttcGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCAc                                                                                                                       <  1:2001933/150‑1 (MQ=255)

                                 ttcGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTc                                                                                                                    >  2:8026473/1‑150 (MQ=255)

                                       gTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCt                                                                                                              >  2:8190904/1‑150 (MQ=255)

                                          cTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGc                                                                                                           <  2:6820415/150‑1 (MQ=255)

                                           ttGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGcc                                                                                                          >  2:7332921/1‑150 (MQ=255)

                                             gagaAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTa                                                                                                        >  2:6672387/1‑150 (MQ=255)

                                                   ttCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatg                                                                                                  <  2:2651084/150‑1 (MQ=255)

                                                   ttCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATGGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatg                                                                                                  <  1:4396406/150‑1 (MQ=255)

                                                    tCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatgg                                                                                                 <  2:6759618/150‑1 (MQ=255)

                                                      gATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATggatggat                                                                                               <  2:7722979/150‑1 (MQ=255)

                                                         atCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGgatgat                                                                                            >  1:6368792/1‑150 (MQ=255)

                                                          tCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTAGGGATGGATGAtt                                                                                           >  1:2562057/1‑150 (MQ=255)

                                                            cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                         <  2:2266062/150‑1 (MQ=255)

                                                            cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                         >  1:7855702/1‑150 (MQ=255)

                                                            cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                         <  1:2953636/150‑1 (MQ=255)

                                                             acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                        <  2:4468267/150‑1 (MQ=255)

                                                             acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                        >  1:1786841/1‑150 (MQ=255)

                                                             acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                        <  1:481269/150‑1 (MQ=255)

                                                             acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                        >  2:5885918/1‑150 (MQ=255)

                                                              tgaagataGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGAGTgggg                                                                                       >  2:3356342/8‑150 (MQ=255)

                                                                 ggACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCt                                                                                    >  2:7138954/1‑150 (MQ=255)

                                                                  gACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTc                                                                                   <  1:4696655/150‑1 (MQ=255)

                                                                    cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                 <  2:3935356/150‑1 (MQ=255)

                                                                    cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                 >  1:8758094/1‑150 (MQ=255)

                                                                    cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                 >  2:223865/1‑150 (MQ=255)

                                                                      gCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGCATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCgaga                                                                               <  1:3170292/150‑1 (MQ=255)

                                                                             cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                        <  1:3883149/150‑1 (MQ=255)

                                                                             cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                        <  1:8548900/150‑1 (MQ=255)

                                                                             cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                        <  1:3418969/150‑1 (MQ=255)

                                                                               gTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCgaga                                                                      <  1:1609489/150‑1 (MQ=255)

                                                                                    ccAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATc                                                                 >  1:1191976/1‑150 (MQ=255)

                                                                                     cAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATcc                                                                >  2:4400639/1‑150 (MQ=255)

                                                                                     cAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATcc                                                                <  1:3287875/150‑1 (MQ=255)

                                                                                      aaTCGGTATCAGCTATCCACAAGATTGGGGCGAGCGCACAATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGAACTTCCACTTCATGCCTGGCCAATGGATGGATGATTGGGGGCTCGAGATTAAAGAGAGCATCCt                                                               >  2:5059265/1‑150 (MQ=255)

                                                                                        tCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCGTgt                                                             >  1:5147180/1‑149 (MQ=255)

                                                                                        tCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGa                                                             >  1:574595/1‑150 (MQ=255)

                                                                                         cGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGAt                                                            <  1:2344498/150‑1 (MQ=255)

                                                                                          ggTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATc                                                           >  2:253506/1‑150 (MQ=255)

                                                                                           gcctgtccTATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCa                                                          <  2:8628963/143‑1 (MQ=255)

                                                                                             ctgtccTATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCAcc                                                        <  1:1389601/145‑2 (MQ=255)

                                                                                              tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                       <  1:5134286/150‑1 (MQ=255)

                                                                                                tccTATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGaa                                                     >  1:7090556/3‑149 (MQ=255)

                                                                                                  cTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATAc                                                   >  2:3040321/1‑150 (MQ=255)

                                                                                                   tATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAAca                                                  >  1:5107315/1‑149 (MQ=255)

                                                                                                    aTCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTg                                                 >  2:750063/1‑150 (MQ=255)

                                                                                                    aTCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTg                                                 >  2:544094/1‑150 (MQ=255)

                                                                                                    aTCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTg                                                 <  1:3959043/150‑1 (MQ=255)

                                                                                                      ccaccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTggg                                               <  1:9126539/150‑1 (MQ=255)

                                                                                                      ccaccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTggg                                               <  2:5604057/150‑1 (MQ=255)

                                                                                                        accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                             >  1:6265270/1‑150 (MQ=255)

                                                                                                        accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                             <  2:6909231/150‑1 (MQ=255)

                                                                                                        accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                             <  2:5305160/150‑1 (MQ=255)

                                                                                                        accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                             >  1:6754781/1‑150 (MQ=255)

                                                                                                         ccggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTc                                            >  1:7199583/7‑150 (MQ=255)

                                                                                                           ggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCga                                          <  1:8071018/146‑1 (MQ=255)

                                                                                                           aGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCga                                          <  2:5737116/150‑1 (MQ=255)

                                                                                                             aTTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCgaga                                        <  1:8142544/150‑1 (MQ=255)

                                                                                                               tGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACg                                      <  2:6202926/150‑1 (MQ=255)

                                                                                                               tGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCgaatgc                                      >  2:4042949/1‑146 (MQ=255)

                                                                                                                 gggCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGtt                                    <  1:8649828/150‑1 (MQ=255)

                                                                                                                         gcACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                            <  1:8920876/150‑1 (MQ=255)

                                                                                                                           aCCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGAct                          >  1:8214616/1‑150 (MQ=255)

                                                                                                                             cATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGActcc                        >  2:7202767/1‑150 (MQ=255)

                                                                                                                             cATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGActcc                        >  1:5518840/1‑150 (MQ=255)

                                                                                                                                   ccTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCaa                  >  1:7395194/1‑150 (MQ=255)

                                                                                                                                   ccTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCaa                  <  2:6407774/150‑1 (MQ=255)

                                                                                                                                          ccGAGCGACAACATCGTGCTCAAACCTGGTATGAACTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc           <  1:6672871/150‑1 (MQ=255)

                                                                                                                                          ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                            <  2:1928268/133‑1 (MQ=255)

                                                                                                                                          ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc           <  2:2139831/150‑1 (MQ=255)

                                                                                                                                          ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc           <  1:5407942/150‑1 (MQ=255)

                                                                                                                                          ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc           <  2:6057332/150‑1 (MQ=255)

                                                                                                                                          ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc           >  2:3782811/1‑150 (MQ=255)

                                                                                                                                            gAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGt         >  2:2375335/1‑150 (MQ=255)

                                                                                                                                            gAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGt         <  1:6456449/150‑1 (MQ=255)

                                                                                                                                               cgcctacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATTGATGGATGATTGGGGGCTCGAGATTTCCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa      <  1:2821426/145‑1 (MQ=255)

                                                                                                                                               cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa      <  2:4356175/150‑1 (MQ=255)

                                                                                                                                               cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa      <  2:1163615/150‑1 (MQ=255)

                                                                                                                                               cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTAGGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa      <  2:9081540/150‑1 (MQ=255)

                                                                                                                                                gACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAg     <  2:4729038/150‑1 (MQ=255)

                                                                                                                                                gACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAg     >  2:1125113/1‑150 (MQ=255)

                                                                                                                                                gACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAg     >  1:7369364/1‑150 (MQ=255)

GGCAACACCACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGAC  >  NC_002947/5030594‑5030890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`