BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,030,745	G→C	6.9%	S348T (AGC→ACC) ‡	PP_4432 →	Xaa‑Pro aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,030,745	0	G	C	6.9%	968.0 / 45.3	291	S348T (AGC→ACC) ‡	PP_4432	Xaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand): ref base G (123/147); new base C (12/8); total (135/156)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 292 total aligned reads displayed.

AACACCACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGAC  >  NC_002947/5030597‑5030894

aaCACCACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGc                                                                                                                                                      >  1:1092556/1‑150 (MQ=255)

   accacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTg                                                                                                                                                   >  2:2944172/1‑150 (MQ=255)

    ccacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGc                                                                                                                                                  <  1:565420/150‑1 (MQ=255)

     cacTGGTGATATCGCACGTGCCTTCTTCGGCGTGTTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCt                                                                                                                                                 <  2:3645827/150‑1 (MQ=255)

     cacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCt                                                                                                                                                 <  2:1794611/150‑1 (MQ=255)

     cacTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCt                                                                                                                                                 >  2:4402409/1‑150 (MQ=255)

      acTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTc                                                                                                                                                <  1:83016/150‑1 (MQ=255)

          gTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAAc                                                                                                                                            <  2:3476258/150‑1 (MQ=255)

             atatCGCACGTGCCGTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTg                                                                                                                                         <  2:1365723/150‑1 (MQ=255)

              tatCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTgg                                                                                                                                        <  2:2796158/150‑1 (MQ=255)

               atCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGt                                                                                                                                       >  1:8410497/1‑150 (MQ=255)

                  gCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATg                                                                                                                                    <  1:6970152/150‑1 (MQ=255)

                        gCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTc                                                                                                                              >  1:305988/1‑150 (MQ=255)

                        gCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTc                                                                                                                              <  1:3776326/150‑1 (MQ=255)

                          cttcttCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCa                                                                                                                            >  1:1719442/1‑150 (MQ=255)

                           ttcttcGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCAc                                                                                                                           <  1:2001933/150‑1 (MQ=255)

                              ttcGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTc                                                                                                                        >  2:8026473/1‑150 (MQ=255)

                                    gTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCt                                                                                                                  >  2:8190904/1‑150 (MQ=255)

                                        ttGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGcc                                                                                                              >  2:7332921/1‑150 (MQ=255)

                                            gaAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatg                                                                                                          <  2:1072045/150‑1 (MQ=255)

                                              aGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatgga                                                                                                        <  1:9077901/150‑1 (MQ=255)

                                               gTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggat                                                                                                       <  1:5448511/150‑1 (MQ=255)

                                               gTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCGGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggat                                                                                                       <  2:4675190/150‑1 (MQ=255)

                                                ttCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatg                                                                                                      <  2:2651084/150‑1 (MQ=255)

                                                ttCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATGGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatg                                                                                                      <  1:4396406/150‑1 (MQ=255)

                                                 tCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatgg                                                                                                     <  2:6759618/150‑1 (MQ=255)

                                                   gATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATggatggat                                                                                                   <  2:7722979/150‑1 (MQ=255)

                                                     tatCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGgatga                                                                                                 <  1:8704839/150‑1 (MQ=255)

                                                      atCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGgatgat                                                                                                >  1:6368792/1‑150 (MQ=255)

                                                       tCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTAGGGATGGATGAtt                                                                                               >  1:2562057/1‑150 (MQ=255)

                                                         cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                             >  1:7855702/1‑150 (MQ=255)

                                                         cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                             <  2:2266062/150‑1 (MQ=255)

                                                         cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                             <  1:2953636/150‑1 (MQ=255)

                                                         cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                             >  2:6864098/1‑150 (MQ=255)

                                                          acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                            >  1:1786841/1‑150 (MQ=255)

                                                          acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                            <  2:4468322/150‑1 (MQ=255)

                                                          acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                            <  1:481269/150‑1 (MQ=255)

                                                          acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                            >  2:6096574/1‑150 (MQ=255)

                                                               gACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTc                                                                                       <  1:4696655/150‑1 (MQ=255)

                                                                 cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                     <  2:5315480/150‑1 (MQ=255)

                                                                 cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                     <  2:3280609/150‑1 (MQ=255)

                                                                 cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                     >  2:223865/1‑150 (MQ=255)

                                                                  aGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCgag                                                                                    <  2:5271023/150‑1 (MQ=255)

                                                                          cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                            <  1:3418969/150‑1 (MQ=255)

                                                                          cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                            <  1:3883149/150‑1 (MQ=255)

                                                                          cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                            <  1:8548900/150‑1 (MQ=255)

                                                                            gTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCgaga                                                                          <  1:1609489/150‑1 (MQ=255)

                                                                                 ccAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATc                                                                     >  1:1191976/1‑150 (MQ=255)

                                                                                 ccAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATc                                                                     >  2:6000289/1‑150 (MQ=255)

                                                                                  cAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATcc                                                                    <  1:3287875/150‑1 (MQ=255)

                                                                                   aaTCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCt                                                                   >  2:6472472/1‑150 (MQ=255)

                                                                                   aaTCGGTATCAGCTATCCACAAGATTGGGGCGAGCGCACAATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGAACTTCCACTTCATGCCTGGCCAATGGATGGATGATTGGGGGCTCGAGATTAAAGAGAGCATCCt                                                                   >  2:5059265/1‑150 (MQ=255)

                                                                                     tCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGa                                                                 >  1:574595/1‑150 (MQ=255)

                                                                                      cGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGAt                                                                <  1:2344498/150‑1 (MQ=255)

                                                                                       ggTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATc                                                               >  2:253506/1‑150 (MQ=255)

                                                                                       ggTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATc                                                               >  1:7671189/1‑150 (MQ=255)

                                                                                        gcctgtccTATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCa                                                              <  2:8628963/143‑1 (MQ=255)

                                                                                          ctgtccTATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCAcc                                                            <  1:1389601/145‑2 (MQ=255)

                                                                                           tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                           <  2:1879845/150‑1 (MQ=255)

                                                                                           tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                           <  1:7425607/150‑1 (MQ=255)

                                                                                           tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                           <  1:5134286/150‑1 (MQ=255)

                                                                                             tccTATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGaa                                                         >  1:7090556/3‑149 (MQ=255)

                                                                                               cTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATAc                                                       >  2:3039819/1‑150 (MQ=255)

                                                                                                tATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAAca                                                      >  1:5107315/1‑149 (MQ=255)

                                                                                                tATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACt                                                      >  2:4930929/1‑150 (MQ=255)

                                                                                                tATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCGATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACt                                                      >  2:5628785/1‑150 (MQ=255)

                                                                                                 aTCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTg                                                     >  2:750063/1‑150 (MQ=255)

                                                                                                 aTCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTg                                                     <  1:3959043/150‑1 (MQ=255)

                                                                                                    caccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTgggg                                                  >  2:4544410/1‑150 (MQ=255)

                                                                                                     accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                 >  1:6265270/1‑150 (MQ=255)

                                                                                                     accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                 >  1:6754781/1‑150 (MQ=255)

                                                                                                        ggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCga                                              >  1:3172236/5‑150 (MQ=255)

                                                                                                        ggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCga                                              <  1:8071018/146‑1 (MQ=255)

                                                                                                          aTTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCgaga                                            <  1:8142544/150‑1 (MQ=255)

                                                                                                            tGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCgaatgc                                          >  2:5475152/1‑146 (MQ=255)

                                                                                                                gCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCCTGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCt                                      <  2:4058509/150‑1 (MQ=255)

                                                                                                                gCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCt                                      >  1:7213270/1‑150 (MQ=255)

                                                                                                                    gcgcACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTc                                  <  2:393667/150‑1 (MQ=255)

                                                                                                                      gcACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                                <  1:8920876/150‑1 (MQ=255)

                                                                                                                       cACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGAc                               <  2:1142458/150‑1 (MQ=255)

                                                                                                                        aCCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGAct                              >  1:8214616/1‑150 (MQ=255)

                                                                                                                          cATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGActcc                            >  2:7202767/1‑150 (MQ=255)

                                                                                                                             gAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCg                         <  1:8807365/150‑1 (MQ=255)

                                                                                                                                 cTGCGCCCGAGCGACAACAGCGTGCTAAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAg                     >  2:6943189/1‑150 (MQ=255)

                                                                                                                                    cgcCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCtt                  <  1:519280/150‑1 (MQ=255)

                                                                                                                                       ccGAGCGACAACATCGTGCTCAAACCTGGTATGAACTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc               <  1:6672871/150‑1 (MQ=255)

                                                                                                                                       ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc               >  2:3782811/1‑150 (MQ=255)

                                                                                                                                       ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc               <  2:2139831/150‑1 (MQ=255)

                                                                                                                                         gAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGt             >  2:2375335/1‑150 (MQ=255)

                                                                                                                                         gAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGt             <  1:6456449/150‑1 (MQ=255)

                                                                                                                                           gCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCa           >  2:6684392/1‑150 (MQ=255)

                                                                                                                                            cgcctacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATTGATGGATGATTGGGGGCTCGAGATTTCCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa          <  1:2821426/145‑1 (MQ=255)

                                                                                                                                            cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa          <  2:4356398/150‑1 (MQ=255)

                                                                                                                                            cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa          <  2:1163615/150‑1 (MQ=255)

                                                                                                                                            cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa          <  1:7370602/150‑1 (MQ=255)

                                                                                                                                            cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTAGGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa          <  2:9081540/150‑1 (MQ=255)

                                                                                                                                               caacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCagtcc       <  2:6213615/150‑5 (MQ=255)

                                                                                                                                                  caGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTg    >  2:5747733/1‑150 (MQ=255)

                                                                                                                                                   aGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGa   >  2:5860046/1‑150 (MQ=255)

                                                                                                                                                    gCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGAc  <  2:7145524/150‑1 (MQ=255)

AACACCACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGAC  >  NC_002947/5030597‑5030894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`