BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,030,752	C→G	7.0%	L350L (CTC→CTG) ‡	PP_4432 →	Xaa‑Pro aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,030,752	0	C	G	7.0%	950.0 / 45.3	288	L350L (CTC→CTG) ‡	PP_4432	Xaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand): ref base C (120/145); new base G (12/8); total (134/154)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 289 total aligned reads displayed.

ACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAG  >  NC_002947/5030603‑5030900

    gTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAAc                                                                                                                                                  <  1:1271169/150‑1 (MQ=255)

    gTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAAc                                                                                                                                                  <  2:3476258/150‑1 (MQ=255)

    gTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAAc                                                                                                                                                  >  2:3664484/1‑150 (MQ=255)

       atatCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTg                                                                                                                                               <  2:7543559/150‑1 (MQ=255)

        tatCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTgg                                                                                                                                              <  2:2796158/150‑1 (MQ=255)

         atCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGt                                                                                                                                             <  1:5121972/150‑1 (MQ=255)

                    cttcttCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCa                                                                                                                                  <  1:2925341/150‑1 (MQ=255)

                     ttcttcGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCAc                                                                                                                                 >  2:5918561/1‑150 (MQ=255)

                             cGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGcc                                                                                                                         >  1:3221762/1‑150 (MQ=255)

                              gTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCt                                                                                                                        <  1:429928/150‑1 (MQ=255)

                                gCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTgg                                                                                                                      <  1:2987307/150‑1 (MQ=255)

                                  ttGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGTGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGcc                                                                                                                    >  1:3836078/1‑150 (MQ=255)

                                   tGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCt                                                                                                                   >  2:3866934/1‑150 (MQ=255)

                                    gagaAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTa                                                                                                                  >  2:6672387/1‑150 (MQ=255)

                                     agaAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTat                                                                                                                 >  1:6557436/1‑150 (MQ=255)

                                      gaAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatg                                                                                                                <  2:7492500/150‑1 (MQ=255)

                                      gaAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatg                                                                                                                <  2:1072045/150‑1 (MQ=255)

                                        aGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatgga                                                                                                              <  1:9077901/150‑1 (MQ=255)

                                         gTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCGGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggat                                                                                                             <  2:4675190/150‑1 (MQ=255)

                                          ttCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatg                                                                                                            >  1:2210737/1‑150 (MQ=255)

                                          ttCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatg                                                                                                            <  2:2651084/150‑1 (MQ=255)

                                          ttCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatg                                                                                                            <  2:1831272/150‑1 (MQ=255)

                                           tCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatgg                                                                                                           <  1:4402409/150‑1 (MQ=255)

                                           tCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTatggatgg                                                                                                           <  2:6759618/150‑1 (MQ=255)

                                              atatCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGgatggatg                                                                                                        >  1:8295894/1‑150 (MQ=255)

                                               tatCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGgatga                                                                                                       <  1:8704839/150‑1 (MQ=255)

                                                 tCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGAtt                                                                                                     <  2:7190787/150‑1 (MQ=255)

                                                   cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                                   <  2:2266062/150‑1 (MQ=255)

                                                   cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                                   >  2:6864098/1‑150 (MQ=255)

                                                   cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                                   >  2:4399295/1‑150 (MQ=255)

                                                   cacaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTgg                                                                                                   >  1:8204182/1‑150 (MQ=255)

                                                    acaAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggg                                                                                                  <  2:4468322/150‑1 (MQ=255)

                                                      aaGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTggggg                                                                                                <  2:8002133/150‑1 (MQ=255)

                                                        ggACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCt                                                                                              >  1:1710194/1‑150 (MQ=255)

                                                           cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                           <  2:4000735/150‑1 (MQ=255)

                                                           cAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCga                                                                                           >  2:223865/1‑150 (MQ=255)

                                                                    cGGTTACCCAATCGGTATCAGCTCTCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                                  <  1:7062958/150‑1 (MQ=255)

                                                                    cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                                  <  1:3070635/150‑1 (MQ=255)

                                                                    cGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCga                                                                                  <  1:3418729/150‑1 (MQ=255)

                                                                      gTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCgaga                                                                                <  1:1158012/150‑1 (MQ=255)

                                                                      gTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGCATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCgaga                                                                                <  1:8622103/150‑1 (MQ=255)

                                                                           ccAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATc                                                                           >  1:5094827/1‑150 (MQ=255)

                                                                             aaTCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCt                                                                         >  2:2967253/1‑150 (MQ=255)

                                                                               tCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCGTgt                                                                       >  1:5147180/1‑149 (MQ=255)

                                                                               tCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGa                                                                       >  1:574595/1‑150 (MQ=255)

                                                                               tCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGa                                                                       <  1:1728622/150‑1 (MQ=255)

                                                                                 ggTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATc                                                                     >  2:253506/1‑150 (MQ=255)

                                                                                     tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                                 >  1:6366832/1‑150 (MQ=255)

                                                                                     tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                                 <  2:1879845/150‑1 (MQ=255)

                                                                                       tccTATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGaa                                                               >  1:7433908/3‑149 (MQ=255)

                                                                                       aGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGAt                                                               >  1:6417252/1‑150 (MQ=255)

                                                                                       aGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACAGAt                                                               >  1:158186/1‑150 (MQ=255)

                                                                                        gCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATa                                                              >  1:3693395/1‑150 (MQ=255)

                                                                                         cTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATAc                                                             >  2:3040321/1‑150 (MQ=255)

                                                                                          tATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACt                                                            >  1:4724692/1‑150 (MQ=255)

                                                                                          tATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACt                                                            >  2:4930929/1‑150 (MQ=255)

                                                                                          tATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCGATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACt                                                            >  2:5628785/1‑150 (MQ=255)

                                                                                            tCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAgg                                                          >  1:2537756/1‑150 (MQ=255)

                                                                                             ccaccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTggg                                                         <  2:8109853/150‑1 (MQ=255)

                                                                                             ccaccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTggg                                                         <  2:1284564/150‑1 (MQ=255)

                                                                                              caccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTgggg                                                        >  2:4544410/1‑150 (MQ=255)

                                                                                               gccggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGt                                                       >  1:8011057/8‑150 (MQ=255)

                                                                                               accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                       <  2:5305160/150‑1 (MQ=255)

                                                                                               accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                       >  1:8360337/1‑150 (MQ=255)

                                                                                               accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                       >  1:6265270/1‑150 (MQ=255)

                                                                                               accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                       >  2:1595099/1‑150 (MQ=255)

                                                                                                ccggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTc                                                      >  1:7199583/7‑150 (MQ=255)

                                                                                                 caGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCg                                                     <  1:8117381/150‑1 (MQ=255)

                                                                                                  aGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCga                                                    <  1:8436342/150‑1 (MQ=255)

                                                                                                    actGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCgaat                                                  <  2:4149420/148‑3 (MQ=255)

                                                                                                     ctGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCgaatg                                                 <  1:3942309/149‑4 (MQ=255)

                                                                                                      tGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACg                                                <  2:6202926/150‑1 (MQ=255)

                                                                                                      tGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCgaatgc                                                >  2:5475152/1‑146 (MQ=255)

                                                                                                          gCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCt                                            >  1:548101/1‑150 (MQ=255)

                                                                                                                gcACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                                      <  1:8920876/150‑1 (MQ=255)

                                                                                                                 cACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGAc                                     <  2:1142458/150‑1 (MQ=255)

                                                                                                                 cACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGAc                                     <  2:7332081/150‑1 (MQ=255)

                                                                                                                    cATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTCTGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGActcc                                  >  1:6907256/1‑150 (MQ=255)

                                                                                                                       gAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCg                               <  1:8807365/150‑1 (MQ=255)

                                                                                                                          ccTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCaa                            <  2:6407774/150‑1 (MQ=255)

                                                                                                                          ccTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCaa                            >  1:7395194/1‑150 (MQ=255)

                                                                                                                           cTGCGCCCGAGCGACAACAGCGTGCTAAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAg                           >  2:6943189/1‑150 (MQ=255)

                                                                                                                            tGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGc                          >  1:2704373/1‑150 (MQ=255)

                                                                                                                                 ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                                      >  1:1928268/1‑133 (MQ=255)

                                                                                                                                 ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc                     <  2:5094827/150‑1 (MQ=255)

                                                                                                                                 ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc                     <  1:5407942/150‑1 (MQ=255)

                                                                                                                                 ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc                     <  2:2139831/150‑1 (MQ=255)

                                                                                                                                   gAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGt                   >  2:2375335/1‑150 (MQ=255)

                                                                                                                                      cGACAACAGCGTGTTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa                <  1:6703827/150‑1 (MQ=255)

                                                                                                                                      cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTAGGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa                <  2:9081540/150‑1 (MQ=255)

                                                                                                                                       gACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAg               >  1:7369364/1‑150 (MQ=255)

                                                                                                                                            caGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTg          >  2:5747733/1‑150 (MQ=255)

                                                                                                                                             aGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTTTGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGa         >  2:6074958/1‑150 (MQ=255)

                                                                                                                                             aGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGa         >  2:5860046/1‑150 (MQ=255)

                                                                                                                                              gCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGAc        <  2:7145524/150‑1 (MQ=255)

                                                                                                                                                gTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGa      <  1:4475404/150‑1 (MQ=255)

                                                                                                                                                   cTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGaaaa   >  2:8788480/1‑150 (MQ=255)

                                                                                                                                                    tCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTc                                                                                             <  2:8371466/59‑1 (MQ=255)

                                                                                                                                                    tCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAg  >  2:3321255/1‑150 (MQ=255)

                                                                                                                                                    tCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAg  >  1:775738/1‑150 (MQ=255)

ACTGGTGATATCGCACGTGCCTTCTTCGGCGTGCTTGAGAAGTTCGATATCCACAAGGACAGCCGTTGCGGTTACCCAATCGGTATCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAG  >  NC_002947/5030603‑5030900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`