BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,030,836	T→C	5.8%	T378T (ACT→ACC)	PP_4432 →	Xaa‑Pro aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,030,836	0	T	C	5.8%	946.6 / 28.6	295	T378T (ACT→ACC)	PP_4432	Xaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand): ref base T (145/132); new base C (11/6); total (157/138)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 301 total aligned reads displayed.

TCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTC  >  NC_002947/5030688‑5030985

tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                                                                                                                      >  1:6366832/1‑150 (MQ=255)

tCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTg                                                                                                                                                      <  1:7425607/150‑1 (MQ=255)

   gCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATa                                                                                                                                                   >  1:8508856/1‑150 (MQ=255)

   gCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATa                                                                                                                                                   >  1:3693395/1‑150 (MQ=255)

     tATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAAca                                                                                                                                                 <  1:299921/150‑2 (MQ=255)

     tATCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCCGCATGACCTTTCATTACATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAAca                                                                                                                                                 <  1:8277940/150‑2 (MQ=255)

     tATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACt                                                                                                                                                 >  2:4930929/1‑150 (MQ=255)

       tCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAgg                                                                                                                                               >  1:6695905/1‑150 (MQ=255)

       tCCGCCGGACTGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAgg                                                                                                                                               >  1:2537756/1‑150 (MQ=255)

        ccaccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTggg                                                                                                                                              <  1:9126539/150‑1 (MQ=255)

         caccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTgggg                                                                                                                                             >  2:4544410/1‑150 (MQ=255)

         caccaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTgggg                                                                                                                                             >  2:3732098/1‑150 (MQ=255)

          accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                                                                                                            >  1:6265270/1‑150 (MQ=255)

          accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                                                                                                            >  2:1595099/1‑150 (MQ=255)

          accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                                                                                                            <  2:5305160/150‑1 (MQ=255)

          accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                                                                                                            <  2:6909231/150‑1 (MQ=255)

          accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGt                                                                                                                                            >  2:805329/1‑150 (MQ=255)

          accaGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCGATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCAACCTGATCACTGATACTGGGGt                                                                                                                                            >  2:2398548/1‑150 (MQ=255)

           ccggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTc                                                                                                                                           >  1:7199583/7‑150 (MQ=255)

            caGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCg                                                                                                                                          <  2:1341508/150‑1 (MQ=255)

            caGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCg                                                                                                                                          <  1:8117381/150‑1 (MQ=255)

             ggactGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCga                                                                                                                                         >  1:3172236/5‑150 (MQ=255)

             aGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCga                                                                                                                                         <  2:5737116/150‑1 (MQ=255)

               actGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCgaat                                                                                                                                       <  2:4149420/148‑3 (MQ=255)

                 tGGGGCGAACGCACCATGAGCCTGCGTCCCGGCGACCGCACGGAATTGCAGCCCGGCATGACCTTTCATTTCATGACAGGTCTCTGGCTGGAAACCATGGGGCTCGAGATTACCGAGAGCATCGTGATCACCGAAACAGGCGTCgaatgc                                                                                                                                     <  1:1656029/150‑5 (MQ=255)

                           gcACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                                                                                                                           <  2:5588792/150‑1 (MQ=255)

                           gcACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                                                                                                                           <  1:4318263/150‑1 (MQ=255)

                           gcACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                                                                                                                           <  1:8920876/150‑1 (MQ=255)

                            cACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGAc                                                                                                                          <  2:7332081/150‑1 (MQ=255)

                               cATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTCTGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGActcc                                                                                                                       >  1:6907256/1‑150 (MQ=255)

                                aTGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGActcct                                                                                                                      >  1:8707244/1‑150 (MQ=255)

                                     ccTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCaa                                                                                                                 <  1:7383089/150‑1 (MQ=255)

                                         cgcCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCtt                                                                                                             <  1:519280/150‑1 (MQ=255)

                                            ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGa                                                                                                                           >  1:1928268/1‑133 (MQ=255)

                                            ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc                                                                                                          <  2:2139831/150‑1 (MQ=255)

                                            ccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc                                                                                                          <  1:5407942/150‑1 (MQ=255)

                                                 cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa                                                                                                     <  2:5787477/150‑1 (MQ=255)

                                                 cGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa                                                                                                     <  2:1163615/150‑1 (MQ=255)

                                                  gACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAg                                                                                                    >  2:1125113/1‑150 (MQ=255)

                                                    caacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCagtcc                                                                                                  <  2:6213615/150‑5 (MQ=255)

                                                        aGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTTTGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGa                                                                                              >  2:6074958/1‑150 (MQ=255)

                                                        aGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGa                                                                                              >  2:5860046/1‑150 (MQ=255)

                                                         gCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGAc                                                                                             <  2:7145524/150‑1 (MQ=255)

                                                           gTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGa                                                                                           >  2:1823751/1‑150 (MQ=255)

                                                           gTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGa                                                                                           <  1:4475404/150‑1 (MQ=255)

                                                              cTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGaaaa                                                                                        >  2:8788480/1‑150 (MQ=255)

                                                                 aaaCCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGtt                                                                                     >  1:574583/1‑150 (MQ=255)

                                                                     cTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCa                                                                                 >  2:6146343/1‑150 (MQ=255)

                                                                         tATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTTGCAAACTTTTCGTCAGTCCTTGACGAAAAGCTTGCGAgga                                                                             <  2:6557436/150‑3 (MQ=37)

                                                                             aCCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTg                                                                         <  2:1719442/150‑1 (MQ=255)

                                                                             aCCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTg                                                                         <  1:7093033/150‑1 (MQ=255)

                                                                               cTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGa                                                                       >  1:5328171/1‑150 (MQ=255)

                                                                               cTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGa                                                                       <  1:7332921/150‑1 (MQ=255)

                                                                               cTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGa                                                                       >  1:6602247/1‑150 (MQ=255)

                                                                                ttCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAg                                                                      <  1:2713963/150‑1 (MQ=255)

                                                                                  ccACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGtt                                                                    >  2:232283/1‑150 (MQ=255)

                                                                                  ccACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGtt                                                                    >  1:2867653/1‑150 (MQ=255)

                                                                                   cACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTta                                                                   >  2:2339898/1‑150 (MQ=255)

                                                                                    aCTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTtat                                                                  >  2:7778808/1‑150 (MQ=255)

                                                                                     cTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTtata                                                                 <  1:5918561/150‑1 (MQ=255)

                                                                                      gtCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAg                                                                <  1:8190904/149‑1 (MQ=255)

                                                                                        cATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTg                                                              <  1:7138954/150‑1 (MQ=255)

                                                                                           gCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCa                                                           >  1:5857565/1‑150 (MQ=255)

                                                                                              tGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACgg                                                        >  2:8194270/1‑150 (MQ=255)

                                                                                                  cTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTAt                                                    >  2:438390/1‑150 (MQ=255)

                                                                                                   tATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATc                                                   >  1:7609992/1‑150 (MQ=255)

                                                                                                    atggatggATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCt                                                  >  1:2753367/1‑150 (MQ=255)

                                                                                                       gatggatgATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCa                                               <  1:211733/150‑1 (MQ=255)

                                                                                                        atggatgATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAg                                              <  2:8028161/150‑1 (MQ=255)

                                                                                                         tggatgATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGc                                             >  2:8536081/1‑150 (MQ=255)

                                                                                                          ggatgATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCg                                            >  2:2770060/1‑150 (MQ=255)

                                                                                                           gatgatTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGa                                           >  2:6735077/1‑150 (MQ=255)

                                                                                                               atTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAAt                                       >  2:1028760/1‑150 (MQ=255)

                                                                                                                 tGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAAtgt                                     <  1:6864098/150‑1 (MQ=255)

                                                                                                                  gggggCCCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAAtgtg                                    >  1:6423586/1‑150 (MQ=255)

                                                                                                                   ggggCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGa                                   >  1:4104793/1‑150 (MQ=255)

                                                                                                                    gggCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGAt                                  <  1:6096574/150‑1 (MQ=255)

                                                                                                                     ggCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATg                                 <  2:9038856/150‑1 (MQ=255)

                                                                                                                       cTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGt                               >  1:5489748/1‑150 (MQ=255)

                                                                                                                        tCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTg                              >  1:7794862/1‑150 (MQ=255)

                                                                                                                        tCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTg                              >  2:8353902/1‑150 (MQ=255)

                                                                                                                          gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGtt                                                                                     <  2:3865260/93‑1 (MQ=255)

                                                                                                                          gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGtt                                                                                     >  1:3865260/1‑93 (MQ=255)

                                                                                                                          gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGcc                            >  2:4044307/1‑150 (MQ=255)

                                                                                                                          gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGcc                            >  2:5058254/1‑150 (MQ=255)

                                                                                                                            gaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGAAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCtt                          >  2:6673932/1‑150 (MQ=255)

                                                                                                                               tACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAATCTTTTCGTCAAGGACTGACGAAAAGTTTGAAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTaaa                       <  2:9094246/150‑1 (MQ=255)

                                                                                                                                 ccGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGc                     <  1:672259/150‑1 (MQ=255)

                                                                                                                                  cGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCa                    >  2:2611260/1‑150 (MQ=255)

                                                                                                                                    agagCATCCTGATCACTGATACGGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCAcc                  <  2:6368792/150‑1 (MQ=255)

                                                                                                                                      agCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCAt                >  2:3258036/1‑150 (MQ=255)

                                                                                                                                        cATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATCa              <  1:8026473/150‑1 (MQ=255)

                                                                                                                                          tCCTGATCACTGATACTGGGGTCGAGACGTTCTTCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACt            >  1:3437263/1‑150 (MQ=255)

                                                                                                                                           ccTGATCACTTATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAATTTTGCAAGTCGCTGGAGTTATATTGCCACGGCTATATTCAGCGACAATGTGATGGTCCCTTAAAGCACCATCACTc           <  1:5059265/150‑1 (MQ=255)

                                                                                                                                            cTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTTGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTcc          <  2:305988/150‑1 (MQ=255)

                                                                                                                                             tGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCa         >  2:6853528/1‑150 (MQ=255)

                                                                                                                                             tGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCa         <  1:4544410/150‑1 (MQ=255)

                                                                                                                                             tGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCa         <  1:7923516/150‑1 (MQ=255)

                                                                                                                                                    tGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTc  >  2:3412475/1‑150 (MQ=255)

                                                                                                                                                    tGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTc  <  1:1081991/150‑1 (MQ=255)

TCAGCTATCCACCAGATTGGGGCGAGCGCACCATGAGCCTGCGCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTC  >  NC_002947/5030688‑5030985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`