BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,100,333	C→A	6.1%	V343V (GTG→GTT)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,100,333	0	C	A	6.1%	876.9 / 31.2	260	V343V (GTG→GTT)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (117/127); new base A (4/12); total (121/139)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 270 total aligned reads displayed.

TTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTT  >  NC_002947/5100184‑5100482

        gTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGcc                                                                                                                                               >  2:7083280/1‑150 (MQ=255)

          cgacgaTCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCg                                                                                                                                             >  1:1839571/1‑150 (MQ=255)

           gacgaTCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCgg                                                                                                                                            >  2:3760077/1‑150 (MQ=255)

           gacgaTCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCgg                                                                                                                                            >  2:505496/1‑150 (MQ=255)

            acgaTCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGa                                                                                                                                           <  1:5745754/150‑1 (MQ=255)

            acgaTCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGa                                                                                                                                           >  1:18036/1‑150 (MQ=255)

                  ttCGATACGCGGGTAATGTCCCGGTAGTAGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTCTGACCCAACCGACGACGGCGGTGCACCAGTACACCTCGCCCGGACGGTaa                                                                                                                                     >  2:1100658/1‑150 (MQ=255)

                   tCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTCAt                                                                                                                                    >  2:2707828/1‑150 (MQ=255)

                    cGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATc                                                                                                                                   <  2:2910107/150‑1 (MQ=255)

                    cGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATc                                                                                                                                   <  2:1476884/150‑1 (MQ=255)

                      aTACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGa                                                                                                                                 >  1:3092694/1‑150 (MQ=255)

                          gcgGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAacac                                                                                                                             >  2:6052555/1‑150 (MQ=255)

                          gcgGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGAGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAacac                                                                                                                             >  2:8724700/1‑150 (MQ=255)

                             ggTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCg                                                                                                                          >  2:178328/1‑150 (MQ=255)

                              gTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGt                                                                                                                         >  2:6867844/1‑150 (MQ=255)

                                 aTGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCGCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCa                                                                                                                      >  1:3364445/1‑150 (MQ=255)

                                  tGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCAt                                                                                                                     <  1:5394136/150‑1 (MQ=255)

                                  tGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCAt                                                                                                                     >  2:76213/1‑150 (MQ=255)

                                   gTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATg                                                                                                                    >  2:2403157/1‑150 (MQ=255)

                                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                   <  1:3601687/150‑1 (MQ=255)

                                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                   <  2:4188831/150‑1 (MQ=255)

                                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                   <  2:4708106/150‑1 (MQ=255)

                                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                   <  2:5598623/150‑1 (MQ=255)

                                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                   <  2:1723954/150‑1 (MQ=255)

                                      cGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGt                                                                                                                 >  1:4320873/1‑150 (MQ=255)

                                      cGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGt                                                                                                                 <  1:6949352/150‑1 (MQ=255)

                                             ttGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGcgcg                                                                                                          >  2:6513328/1‑150 (MQ=255)

                                               gggCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCgcggc                                                                                                        <  2:8372321/150‑1 (MQ=255)

                                               gggCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCgcggc                                                                                                        >  1:7485870/1‑150 (MQ=255)

                                                ggCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCgcggcg                                                                                                       <  2:6160611/150‑1 (MQ=255)

                                                 gCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTCCATGGGTCAGCGCGGCGt                                                                                                      >  1:5152939/1‑150 (MQ=255)

                                                       cTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCa                                                                                                >  2:1625607/1‑150 (MQ=255)

                                                       cTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCa                                                                                                >  1:738657/1‑150 (MQ=255)

                                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACAccc                                                                                                                           <  2:458749/122‑1 (MQ=255)

                                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                               <  2:3873292/150‑1 (MQ=255)

                                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                               >  1:1042766/1‑150 (MQ=255)

                                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                               <  1:3922727/150‑1 (MQ=255)

                                                         tCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAgg                                                                                              <  1:8859036/150‑1 (MQ=255)

                                                                     gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                  <  2:7290556/150‑1 (MQ=255)

                                                                     gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                  >  1:1544555/1‑150 (MQ=255)

                                                                     gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                  <  2:1922563/150‑1 (MQ=255)

                                                                       ggtggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTgtggt                                                                                <  1:3107813/150‑1 (MQ=255)

                                                                        gtggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTgtggtg                                                                               <  1:2064903/150‑1 (MQ=255)

                                                                          ggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGtgtg                                                                             <  2:8147832/150‑1 (MQ=255)

                                                                          ggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGtgtg                                                                             <  1:3420960/150‑1 (MQ=255)

                                                                           gcgcCGTTGGTCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgc                                                                            <  1:3531068/150‑1 (MQ=255)

                                                                           gcgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgc                                                                            >  1:1986897/1‑150 (MQ=255)

                                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                           <  1:7293605/150‑1 (MQ=255)

                                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                           <  2:8902185/150‑1 (MQ=255)

                                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                           <  2:2035722/150‑1 (MQ=255)

                                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                           >  1:5187950/1‑150 (MQ=255)

                                                                              ccGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagc                                                                         >  1:5315746/1‑150 (MQ=255)

                                                                               cGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagca                                                                        >  2:1134810/1‑150 (MQ=255)

                                                                               cGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagca                                                                        >  2:5680342/1‑150 (MQ=255)

                                                                                  tGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGc                                                                     >  1:7563564/1‑150 (MQ=255)

                                                                                       aGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTc                                                                >  2:7558313/1‑150 (MQ=255)

                                                                                       aGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTc                                                                <  2:2397267/150‑1 (MQ=255)

                                                                                       aGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTc                                                                >  2:622391/1‑150 (MQ=255)

                                                                                        gCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCg                                                               >  1:8121933/1‑150 (MQ=255)

                                                                                         cGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCgg                                                              <  1:926845/150‑1 (MQ=255)

                                                                                          gggCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGc                                                             <  2:2231354/150‑1 (MQ=255)

                                                                                              cGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGc                                                         <  2:8276774/150‑1 (MQ=255)

                                                                                                   aCGATATCGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                    <  2:7278748/150‑1 (MQ=255)

                                                                                                   aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                    <  1:6379960/150‑1 (MQ=255)

                                                                                                   aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                    <  1:2719717/150‑1 (MQ=255)

                                                                                                     gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                  <  1:8613642/150‑1 (MQ=255)

                                                                                                     gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                  >  1:6202321/1‑150 (MQ=255)

                                                                                                     gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                  <  2:4561035/150‑1 (MQ=255)

                                                                                                            cTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCGCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTTAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCGGGAg                                           <  2:3974868/150‑1 (MQ=255)

                                                                                                                 gCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGtata                                      >  1:1331417/1‑150 (MQ=255)

                                                                                                                   cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                    <  2:1330735/150‑1 (MQ=255)

                                                                                                                   cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                    <  1:6074443/150‑1 (MQ=255)

                                                                                                                    tgtgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAg                                   <  2:5808946/150‑1 (MQ=255)

                                                                                                                     gtgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAgg                                  >  1:9074602/1‑150 (MQ=255)

                                                                                                                      tgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGa                                 <  1:5912790/150‑1 (MQ=255)

                                                                                                                      tcaCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGa                                 <  1:4202281/148‑1 (MQ=255)

                                                                                                                          ccAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGaa                             >  2:668923/1‑150 (MQ=255)

                                                                                                                            aaCCGACGTCGGCGGTGCTCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACa                           <  1:4080134/150‑1 (MQ=255)

                                                                                                                            aaCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACa                           >  2:519624/1‑150 (MQ=255)

                                                                                                                             aCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAg                          <  1:2794589/150‑1 (MQ=255)

                                                                                                                                gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                       >  2:5270854/1‑150 (MQ=255)

                                                                                                                                gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                       <  1:2433321/150‑1 (MQ=255)

                                                                                                                                gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                       <  1:7051819/150‑1 (MQ=255)

                                                                                                                                  cGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCct                     <  2:856017/150‑1 (MQ=255)

                                                                                                                                   gTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCctc                    >  1:8283336/1‑150 (MQ=255)

                                                                                                                                     cggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCtt                  <  2:9075126/150‑1 (MQ=255)

                                                                                                                                      ggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTc                 <  1:8222855/150‑1 (MQ=255)

                                                                                                                                      ggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTc                 <  2:7960860/150‑1 (MQ=255)

                                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                       <  2:4786666/143‑2 (MQ=255)

                                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                       >  1:4786666/1‑142 (MQ=255)

                                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                <  2:3289124/150‑2 (MQ=255)

                                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                >  2:1019407/1‑149 (MQ=255)

                                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                <  1:8035112/150‑2 (MQ=255)

                                                                                                                                              aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa         <  1:7824849/150‑1 (MQ=255)

                                                                                                                                              aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa         >  1:2026602/1‑150 (MQ=255)

                                                                                                                                               ccAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAt        >  2:2941179/1‑150 (MQ=255)

                                                                                                                                               ccAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAt        <  1:1100658/150‑1 (MQ=255)

                                                                                                                                                   tACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCt    >  1:8054702/1‑150 (MQ=255)

                                                                                                                                                     cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt  <  2:4122289/150‑1 (MQ=255)

                                                                                                                                                     cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt  <  1:505496/150‑1 (MQ=255)

TTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTT  >  NC_002947/5100184‑5100482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`