BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,100,348	G→A	5.9%	Y338Y (TAC→TAT)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,100,348	0	G	A	5.9%	859.6 / 27.2	271	Y338Y (TAC→TAT)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (111/144); new base A (4/12); total (115/156)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 274 total aligned reads displayed.

TCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCT  >  NC_002947/5100200‑5100497

tCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGt                                                                                                                                                      >  1:7691242/1‑150 (MQ=255)

   tCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAAt                                                                                                                                                   >  2:2707871/1‑150 (MQ=255)

      aTACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGa                                                                                                                                                >  1:3092706/1‑150 (MQ=255)

       tACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGaa                                                                                                                                               >  2:233521/1‑150 (MQ=255)

          gcgGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGAGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAacac                                                                                                                                            >  2:8724700/1‑150 (MQ=255)

             ggTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCg                                                                                                                                         >  1:5808946/1‑150 (MQ=255)

                   gTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATg                                                                                                                                   >  2:2403157/1‑150 (MQ=255)

                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                                  <  2:4188831/150‑1 (MQ=255)

                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                                  <  2:5598623/150‑1 (MQ=255)

                    tCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                                  >  1:7999666/1‑150 (MQ=255)

                      cGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGt                                                                                                                                >  1:4320873/1‑150 (MQ=255)

                             ttGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGcgcg                                                                                                                         >  2:6513328/1‑150 (MQ=255)

                               gggCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCgcggc                                                                                                                       >  1:7485870/1‑150 (MQ=255)

                                      ccTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                                  <  1:246854/132‑1 (MQ=255)

                                      ccTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATgg                                                                                                                                  >  2:246854/1‑132 (MQ=255)

                                       cTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCa                                                                                                               >  2:1625607/1‑150 (MQ=255)

                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACAccc                                                                                                                                          >  1:458749/1‑122 (MQ=255)

                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                              >  1:4133907/1‑150 (MQ=255)

                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                              >  1:1042766/1‑150 (MQ=255)

                                        ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                              <  1:3922727/150‑1 (MQ=255)

                                         tCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTGCATGGGTCAGCGCGGCGTAAACCAgg                                                                                                             <  2:1730557/150‑1 (MQ=255)

                                                 cAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGcc                                                                                                     >  1:3138531/1‑150 (MQ=255)

                                                 cAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGcc                                                                                                     >  2:7450538/1‑150 (MQ=255)

                                                    ggtggtGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgt                                                                                                  <  2:3502833/150‑1 (MQ=255)

                                                     gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCACCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCggtg                                                                                                 >  2:8509426/1‑150 (MQ=255)

                                                     gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                 <  1:2879827/150‑1 (MQ=255)

                                                     gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                 >  1:1544555/1‑150 (MQ=255)

                                                     gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                 <  2:7290556/150‑1 (MQ=255)

                                                       ggtggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTgtggt                                                                                               <  2:1664123/150‑1 (MQ=255)

                                                          ggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGtgtg                                                                                            <  1:3420696/150‑1 (MQ=255)

                                                          ggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGtgtg                                                                                            <  2:8147832/150‑1 (MQ=255)

                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                          <  2:2035722/150‑1 (MQ=255)

                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                          <  2:8902185/150‑1 (MQ=255)

                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                          <  1:7293605/150‑1 (MQ=255)

                                                            cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                          <  2:4636303/150‑1 (MQ=255)

                                                             gcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcag                                                                                         >  2:6961068/1‑150 (MQ=255)

                                                              ccGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagc                                                                                        >  1:5315436/1‑150 (MQ=255)

                                                               cGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGAACCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAAACAGGTAACCGCCTGTGGTGTgcagca                                                                                       >  2:1134101/1‑150 (MQ=255)

                                                                  tGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGc                                                                                    >  1:7563552/1‑150 (MQ=255)

                                                                      cAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCtt                                                                                <  1:8890906/150‑1 (MQ=255)

                                                                       aGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTc                                                                               >  2:7558313/1‑150 (MQ=255)

                                                                         cGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCgg                                                                             <  1:926845/150‑1 (MQ=255)

                                                                          gggCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGc                                                                            <  2:2231354/150‑1 (MQ=255)

                                                                            gCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCtt                                                                          <  2:1194499/150‑1 (MQ=255)

                                                                                  gACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGt                                                                    >  1:5167475/1‑150 (MQ=255)

                                                                                  gACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGt                                                                    <  1:7374498/150‑1 (MQ=255)

                                                                                   aCGATATCGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                   <  2:7278748/150‑1 (MQ=255)

                                                                                   aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                   >  1:8419889/1‑150 (MQ=255)

                                                                                     gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                                 >  1:6202321/1‑150 (MQ=255)

                                                                                            cTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAg                                                          <  2:293407/150‑1 (MQ=255)

                                                                                                 gCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGtata                                                     >  1:1330735/1‑150 (MQ=255)

                                                                                                   cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                   <  1:194780/150‑1 (MQ=255)

                                                                                                   cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                   <  2:1331417/150‑1 (MQ=255)

                                                                                                    tgtgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAg                                                  <  2:4904539/150‑1 (MQ=255)

                                                                                                    tgtgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAg                                                  >  1:6256425/1‑150 (MQ=255)

                                                                                                          ccAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGaa                                            >  2:668923/1‑150 (MQ=255)

                                                                                                          ccAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGaa                                            <  2:7667126/150‑1 (MQ=255)

                                                                                                          ccAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGaa                                            >  2:578933/1‑150 (MQ=255)

                                                                                                          ccAACCGACCACGGCGGTGCACCAGTACACCACGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGaa                                            <  2:1965520/150‑1 (MQ=255)

                                                                                                           cAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAAc                                           >  2:5288524/1‑150 (MQ=255)

                                                                                                            aaCCGACGTCGGCGGTGCTCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACa                                          <  1:4080134/150‑1 (MQ=255)

                                                                                                            aaCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACa                                          >  2:519624/1‑150 (MQ=255)

                                                                                                               cGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcg                                       >  1:3497943/1‑150 (MQ=255)

                                                                                                                gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                                      <  1:7051819/150‑1 (MQ=255)

                                                                                                                   gTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCctc                                   >  1:8283336/1‑150 (MQ=255)

                                                                                                                     cggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCtt                                 <  2:8350752/150‑1 (MQ=255)

                                                                                                                     cggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCtt                                 <  2:9075126/150‑1 (MQ=255)

                                                                                                                     cggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCtt                                 <  1:8724700/150‑1 (MQ=255)

                                                                                                                      ggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTc                                <  1:8222855/150‑1 (MQ=255)

                                                                                                                       gcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCa                               <  2:6168262/150‑1 (MQ=255)

                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                                      >  1:4786666/1‑142 (MQ=255)

                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                                      <  2:4786666/143‑2 (MQ=255)

                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                               <  2:3918133/150‑2 (MQ=255)

                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                               >  2:1019407/1‑149 (MQ=255)

                                                                                                                       gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                               <  2:3289115/150‑2 (MQ=255)

                                                                                                                        cggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAg                              <  2:4562461/150‑1 (MQ=255)

                                                                                                                              aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                        >  1:2026602/1‑150 (MQ=255)

                                                                                                                               ccAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAt                       <  1:6052555/150‑1 (MQ=255)

                                                                                                                                cAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAtt                      >  1:2767946/1‑150 (MQ=255)

                                                                                                                                   tACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCt                   >  1:8054702/1‑150 (MQ=255)

                                                                                                                                     cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                 <  2:4122289/150‑1 (MQ=255)

                                                                                                                                     cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                 <  1:505496/150‑1 (MQ=255)

                                                                                                                                     cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                 <  1:6769843/150‑1 (MQ=255)

                                                                                                                                     cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                 <  1:5366951/150‑1 (MQ=255)

                                                                                                                                      acCTCGCCCGGACGGTCATCGAACACCCGTTCCTGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCGCTTCAGCACCCCTTTCtggg                >  2:5908434/1‑147 (MQ=255)

                                                                                                                                      acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                   >  2:772680/1‑99 (MQ=255)

                                                                                                                                      acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                   <  1:772680/99‑1 (MQ=255)

                                                                                                                                      acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTg                <  1:3606273/150‑1 (MQ=255)

                                                                                                                                       ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg               <  2:3829785/150‑1 (MQ=255)

                                                                                                                                       ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg               <  2:18036/150‑1 (MQ=255)

                                                                                                                                        cTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTggg              >  1:1052356/1‑150 (MQ=255)

                                                                                                                                         tCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAg                                                          >  1:2232232/1‑105 (MQ=255)

                                                                                                                                             ccGGACGGTTATCGAACACCCTTTCATGGGTCAGCGCGGCGTATACCTGGTATCCGCCTGTGGTGTGCAGCACGGCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGACCAGCGCCTCTTCAGCACCCATTTCTTTGGGGgcgc         <  2:6798107/150‑1 (MQ=255)

                                                                                                                                             ccGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                 >  1:1725366/1‑142 (MQ=255)

                                                                                                                                              cGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACACATTTCTTTGGGGGCGCa        >  2:5656669/1‑150 (MQ=255)

                                                                                                                                              cGGACGGTAAACGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCa        >  1:9100728/1‑150 (MQ=255)

                                                                                                                                               ggACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAg       >  1:2378322/1‑150 (MQ=255)

                                                                                                                                                  cGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTgg    <  2:7999666/150‑1 (MQ=255)

                                                                                                                                                   ggTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATGAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGc   <  1:3551984/150‑1 (MQ=255)

                                                                                                                                                   ggTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCAAGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGc   >  2:6055105/1‑150 (MQ=255)

TCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCT  >  NC_002947/5100200‑5100497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`