BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,100,374	G→T	5.4%	L330M (CTG→ATG)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,100,374	0	G	T	5.4%	1017.4 / 28.2	300	L330M (CTG→ATG)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (133/150); new base T (4/13); total (137/163)
Fisher's exact test for biased strand distribution p-value = 7.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

TTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGT  >  NC_002947/5100229‑5100523

ttGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGcgcg                                                                                                                                                   >  2:6513328/1‑150 (MQ=255)

  gggCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCgcggc                                                                                                                                                 <  2:8372321/150‑1 (MQ=255)

  gggCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCgcggc                                                                                                                                                 >  1:3601494/1‑150 (MQ=255)

    gCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGt                                                                                                                                               >  2:4140409/1‑150 (MQ=255)

          cTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCa                                                                                                                                         >  2:1625607/1‑150 (MQ=255)

          cTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGCCAGCGCGGCGTAAACCa                                                                                                                                         <  1:743268/150‑1 (MQ=255)

           ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                                                        >  2:6126225/1‑150 (MQ=255)

           ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                                                        <  2:3873292/150‑1 (MQ=255)

           ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                                                        >  1:1042766/1‑150 (MQ=255)

                  aGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCg                                                                                                                                 >  2:6786599/1‑150 (MQ=255)

                    cAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGcc                                                                                                                               >  1:3138531/1‑150 (MQ=255)

                       ggtggtGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgt                                                                                                                            <  2:3502833/150‑1 (MQ=255)

                        gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                                           <  2:3997213/150‑1 (MQ=255)

                             ggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGtgtg                                                                                                                      <  1:3420960/150‑1 (MQ=255)

                               cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                    <  1:7837796/150‑1 (MQ=255)

                               cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                    >  1:5187969/1‑150 (MQ=255)

                               cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                    <  2:4636303/150‑1 (MQ=255)

                                gcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcag                                                                                                                   >  2:8107541/1‑150 (MQ=255)

                                  cGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagca                                                                                                                 >  2:1134810/1‑150 (MQ=255)

                                  cGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagca                                                                                                                 >  2:5680342/1‑150 (MQ=255)

                                       gCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGccc                                                                                                            <  1:5033134/150‑1 (MQ=255)

                                             gggCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGc                                                                                                      <  2:2231354/150‑1 (MQ=255)

                                                ccGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTg                                                                                                   >  1:911357/1‑150 (MQ=255)

                                                   tAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTTACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCg                                                                                                >  1:3925856/1‑150 (MQ=255)

                                                     gACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGt                                                                                              >  1:462708/1‑150 (MQ=255)

                                                     gACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGt                                                                                              >  1:5167441/1‑150 (MQ=255)

                                                      aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                             >  1:8419889/1‑150 (MQ=255)

                                                      aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                             <  2:7279008/150‑1 (MQ=255)

                                                        gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                                                           >  1:6202321/1‑150 (MQ=255)

                                                               cTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAg                                                                                    <  2:8155786/150‑1 (MQ=255)

                                                                     ccTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaa                                                                              >  1:7335204/1‑150 (MQ=255)

                                                                      cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTATCCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                             <  2:7218315/150‑1 (MQ=255)

                                                                      cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                             >  2:8941145/1‑150 (MQ=255)

                                                                      cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                             <  1:194780/150‑1 (MQ=255)

                                                                      cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                             <  2:1331417/150‑1 (MQ=255)

                                                                      cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                             <  1:6074443/150‑1 (MQ=255)

                                                                        gtgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAgg                                                                           >  1:9074602/1‑150 (MQ=255)

                                                                         tgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGa                                                                          <  1:5912790/150‑1 (MQ=255)

                                                                         tcaCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGa                                                                          <  1:4202281/148‑1 (MQ=255)

                                                                         tcaCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGa                                                                          <  2:5859896/148‑1 (MQ=255)

                                                                          caCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGAt                                                                         <  1:3863230/149‑1 (MQ=255)

                                                                             ccAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGaa                                                                      >  2:578933/1‑150 (MQ=255)

                                                                             ccAACCGACCACGGCGGTGCACCAGTACACCACGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGaa                                                                      <  2:1965520/150‑1 (MQ=255)

                                                                               aaCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACa                                                                    >  2:519624/1‑150 (MQ=255)

                                                                                  cGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcg                                                                 >  1:3497943/1‑150 (MQ=255)

                                                                                   gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                                                                <  1:7051626/150‑1 (MQ=255)

                                                                                   gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                                                                >  2:5270854/1‑150 (MQ=255)

                                                                                     cGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCct                                                              <  2:7410584/150‑1 (MQ=255)

                                                                                        cggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCtt                                                           <  2:5602057/150‑1 (MQ=255)

                                                                                         ggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTc                                                          <  1:8222855/150‑1 (MQ=255)

                                                                                          gcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCa                                                         <  2:6168262/150‑1 (MQ=255)

                                                                                          gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                                                                <  2:4786666/143‑2 (MQ=255)

                                                                                          gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                                                         >  2:1019407/1‑149 (MQ=255)

                                                                                          gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                                                         <  2:3289115/150‑2 (MQ=255)

                                                                                           cggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAg                                                        <  2:4562461/150‑1 (MQ=255)

                                                                                                 aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                                                  >  1:1587689/1‑150 (MQ=255)

                                                                                                 aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                                                  >  1:2026602/1‑150 (MQ=255)

                                                                                                   cAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAtt                                                >  1:2767946/1‑150 (MQ=255)

                                                                                                      tACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCt                                             >  1:8054702/1‑150 (MQ=255)

                                                                                                        cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                           <  1:5366951/150‑1 (MQ=255)

                                                                                                        cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                           <  1:6769843/150‑1 (MQ=255)

                                                                                                        cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                           <  2:6849324/150‑1 (MQ=255)

                                                                                                         acCTCGCCCGGACGGTCATCGAACACCCGTTCCTGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCGCTTCAGCACCCCTTTCtggg                                          >  2:5908434/1‑147 (MQ=255)

                                                                                                         acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                             <  1:772680/99‑1 (MQ=255)

                                                                                                          ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                         <  1:4726771/150‑1 (MQ=255)

                                                                                                          ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                         <  2:18036/150‑1 (MQ=255)

                                                                                                          ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                         <  1:7493738/150‑1 (MQ=255)

                                                                                                          ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                         <  2:7506799/150‑1 (MQ=255)

                                                                                                           cTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTggg                                        <  1:881595/150‑1 (MQ=255)

                                                                                                            tCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAg                                                                                    >  1:2232232/1‑105 (MQ=255)

                                                                                                                ccGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                           <  2:1725366/142‑1 (MQ=255)

                                                                                                                 cGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCa                                  <  1:452761/150‑1 (MQ=255)

                                                                                                                     cGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTgg                              <  2:7691242/150‑1 (MQ=255)

                                                                                                                         aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                          >  2:8591073/1‑150 (MQ=255)

                                                                                                                         aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                          >  2:1229791/1‑150 (MQ=255)

                                                                                                                         aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                          <  1:2402968/150‑1 (MQ=255)

                                                                                                                         aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                          >  1:2475093/1‑150 (MQ=255)

                                                                                                                          aTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGTGGGCGCAGTGGCTGGa                         <  1:1057766/150‑1 (MQ=255)

                                                                                                                          aTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGa                         >  1:295769/1‑150 (MQ=255)

                                                                                                                          aTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGa                         <  1:76213/150‑1 (MQ=255)

                                                                                                                           tCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAg                        >  2:7945329/1‑150 (MQ=255)

                                                                                                                            cGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgc                                                                                                                     >  2:8284413/1‑56 (MQ=255)

                                                                                                                            cGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgc                                                                                                                     <  1:8284413/56‑1 (MQ=255)

                                                                                                                             gAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGc                      <  1:7083280/150‑1 (MQ=255)

                                                                                                                              aaCACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGcc                     >  2:4064600/1‑150 (MQ=255)

                                                                                                                               acacCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCa                    <  2:2853755/150‑1 (MQ=255)

                                                                                                                                 acCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACt                  >  2:4276987/1‑150 (MQ=255)

                                                                                                                                   ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                >  2:3734474/1‑150 (MQ=255)

                                                                                                                                   ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                >  1:1314730/1‑150 (MQ=255)

                                                                                                                                   ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGCAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                >  2:698747/1‑150 (MQ=255)

                                                                                                                                      ttCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAttt                                               >  2:2320419/1‑116 (MQ=255)

                                                                                                                                      ttCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAttt                                               <  1:2320419/116‑1 (MQ=255)

                                                                                                                                      ttCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTtcat             <  1:1462505/150‑1 (MQ=255)

                                                                                                                                       tCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTtcatc            <  1:178328/150‑1 (MQ=255)

                                                                                                                                         aTGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAg          <  2:2030179/150‑1 (MQ=255)

                                                                                                                                              tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt     >  1:639521/1‑150 (MQ=255)

                                                                                                                                              tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt     >  2:4930562/1‑150 (MQ=255)

                                                                                                                                               cAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTc    <  2:6619224/150‑1 (MQ=255)

                                                                                                                                               cAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTc    <  2:3092694/150‑1 (MQ=255)

                                                                                                                                                 gcgcGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGt  <  2:3364445/150‑1 (MQ=255)

TTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGT  >  NC_002947/5100229‑5100523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`