BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,100,387	C→G	5.2%	L325L (CTG→CTC)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,100,387	0	C	G	5.2%	1036.8 / 32.2	306	L325L (CTG→CTC)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (135/154); new base G (4/12); total (139/167)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 309 total aligned reads displayed.

CTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCG  >  NC_002947/5100239‑5100536

cTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCa                                                                                                                                                      >  1:3925648/1‑150 (MQ=255)

 ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                                                                     >  1:1042766/1‑150 (MQ=255)

 ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                                                                     >  2:6126225/1‑150 (MQ=255)

 ttCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAg                                                                                                                                                     >  1:4133907/1‑150 (MQ=255)

  tCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTGCATGGGTCAGCGCGGCGTAAACCAgg                                                                                                                                                    <  2:1730557/150‑1 (MQ=255)

             ggtggtGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgt                                                                                                                                         <  2:3502833/150‑1 (MQ=255)

              gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                                                        >  1:1544555/1‑150 (MQ=255)

              gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                                                        <  2:1922563/150‑1 (MQ=255)

              gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                                                        <  2:7290556/150‑1 (MQ=255)

               tggtggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTgtgg                                                                                                                                       >  2:3805846/1‑150 (MQ=255)

                ggtggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTgtggt                                                                                                                                      <  1:3107374/150‑1 (MQ=255)

                   ggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGtgtg                                                                                                                                   <  1:3420696/150‑1 (MQ=255)

                    gcgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgc                                                                                                                                  >  1:1986897/1‑150 (MQ=255)

                    gcgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgc                                                                                                                                  >  1:6619224/1‑150 (MQ=255)

                     cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                                 <  2:4636303/150‑1 (MQ=255)

                     cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                                 >  1:5187969/1‑150 (MQ=255)

                      gcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcag                                                                                                                                >  2:8107541/1‑150 (MQ=255)

                           tGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGc                                                                                                                           >  1:7563564/1‑150 (MQ=255)

                                aGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTc                                                                                                                      <  2:5645396/150‑1 (MQ=255)

                                 gCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCg                                                                                                                     >  1:8121933/1‑150 (MQ=255)

                                     gCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCtt                                                                                                                 <  2:1194499/150‑1 (MQ=255)

                                      ccGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTg                                                                                                                >  1:911357/1‑150 (MQ=255)

                                           gACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGt                                                                                                           >  1:5167441/1‑150 (MQ=255)

                                           gACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGt                                                                                                           <  1:7374498/150‑1 (MQ=255)

                                            aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                                          <  1:2719717/150‑1 (MQ=255)

                                              gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                                                                        <  2:523248/150‑1 (MQ=255)

                                              gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                                                                        <  1:8613642/150‑1 (MQ=255)

                                              gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                                                                        <  2:2004991/150‑1 (MQ=255)

                                              gATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGa                                                                                                        >  1:6202321/1‑150 (MQ=255)

                                                 ataGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGcc                                                                                                     <  1:8367496/150‑1 (MQ=255)

                                                     cTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAg                                                                                                 <  2:8155786/150‑1 (MQ=255)

                                                         ggCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGtat                                                                                             <  1:1812768/150‑1 (MQ=255)

                                                            cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                                          <  1:6074443/150‑1 (MQ=255)

                                                            cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                                          >  2:8941145/1‑150 (MQ=255)

                                                             tgtgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAg                                                                                         <  2:5808946/150‑1 (MQ=255)

                                                              gtgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAgg                                                                                        >  1:9074602/1‑150 (MQ=255)

                                                               tgACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGa                                                                                       <  1:5912790/150‑1 (MQ=255)

                                                                gACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGAt                                                                                      <  2:1251601/150‑1 (MQ=255)

                                                                caCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGAt                                                                                      <  1:228677/149‑1 (MQ=255)

                                                                caCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGAt                                                                                      <  2:1636829/149‑1 (MQ=255)

                                                                caCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGAt                                                                                      <  1:3863230/149‑1 (MQ=255)

                                                                   ccAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGaa                                                                                   <  2:7667126/150‑1 (MQ=255)

                                                                    cAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAAc                                                                                  >  2:5288524/1‑150 (MQ=255)

                                                                      aCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAg                                                                                <  1:2794589/150‑1 (MQ=255)

                                                                           cGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCct                                                                           <  1:7491578/150‑1 (MQ=255)

                                                                            gTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCctc                                                                          >  1:8283336/1‑150 (MQ=255)

                                                                              cggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCtt                                                                        <  2:8350752/150‑1 (MQ=255)

                                                                               cgcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTc                                                                       <  1:7059864/149‑1 (MQ=2)

                                                                                gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                                                                             <  2:4786666/143‑2 (MQ=255)

                                                                                gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                                                                      <  2:3918133/150‑2 (MQ=255)

                                                                                gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                                                                      >  2:1019407/1‑149 (MQ=255)

                                                                                gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                                                                      <  2:3289115/150‑2 (MQ=255)

                                                                                 cggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAg                                                                     <  2:4562461/150‑1 (MQ=255)

                                                                                       aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                                                               <  1:7824849/150‑1 (MQ=255)

                                                                                       aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                                                               >  1:2026679/1‑150 (MQ=255)

                                                                                            tACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCt                                                          >  1:8054690/1‑150 (MQ=255)

                                                                                            tACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCt                                                          >  1:3494308/1‑150 (MQ=255)

                                                                                              cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                                        <  1:5366951/150‑1 (MQ=255)

                                                                                               acCTCGCCCGGACGGTCATCGAACACCCGTTCCTGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCGCTTCAGCACCCCTTTCtggg                                                       >  2:5908434/1‑147 (MQ=255)

                                                                                               acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                                          <  1:772616/99‑1 (MQ=255)

                                                                                               acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTg                                                       <  2:7485870/150‑1 (MQ=255)

                                                                                                ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                                      <  1:4726771/150‑1 (MQ=255)

                                                                                                ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                                      <  1:4457476/150‑1 (MQ=255)

                                                                                                 cTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTggg                                                     <  1:881595/150‑1 (MQ=255)

                                                                                                      ccGGACGGTTATCGAACACCCTTTCATGGGTCAGCGCGGCGTATACCTGGTATCCGCCTGTGGTGTGCAGCACGGCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGACCAGCGCCTCTTCAGCACCCATTTCTTTGGGGgcgc                                                <  2:6798107/150‑1 (MQ=255)

                                                                                                        ggACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAg                                              >  1:2378322/1‑150 (MQ=255)

                                                                                                           cGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTgg                                           <  2:7691242/150‑1 (MQ=255)

                                                                                                            ggTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATGAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGc                                          <  1:3551984/150‑1 (MQ=255)

                                                                                                               aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                                       >  2:8591073/1‑150 (MQ=255)

                                                                                                               aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                                       >  2:7198723/1‑150 (MQ=255)

                                                                                                               aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                                       <  1:2403157/150‑1 (MQ=255)

                                                                                                                aTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGTGGGCGCAGTGGCTGGa                                      <  1:1057766/150‑1 (MQ=255)

                                                                                                                aTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGa                                      >  1:6964271/1‑150 (MQ=255)

                                                                                                                 tCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAg                                     >  2:7945329/1‑150 (MQ=255)

                                                                                                                 tCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAg                                     >  2:6635625/1‑150 (MQ=255)

                                                                                                                  cGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgc                                                                                                                                  >  2:8284413/1‑56 (MQ=255)

                                                                                                                    aaCACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGcc                                  >  2:4064347/1‑150 (MQ=255)

                                                                                                                     acacCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCa                                 <  2:2853755/150‑1 (MQ=255)

                                                                                                                       acCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACt                               >  1:4833384/1‑150 (MQ=255)

                                                                                                                       acCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACt                               >  2:4276987/1‑150 (MQ=255)

                                                                                                                         ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                             >  2:6892979/1‑150 (MQ=255)

                                                                                                                         ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                             >  1:1314730/1‑150 (MQ=255)

                                                                                                                         ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                             >  2:5723870/1‑150 (MQ=255)

                                                                                                                           gTTCATGGGTCAGCGCGGCGAAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTtca                           <  2:4133907/150‑1 (MQ=255)

                                                                                                                            ttCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAttt                                                            >  2:2320419/1‑116 (MQ=255)

                                                                                                                            ttCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTtcat                          >  1:7144011/1‑150 (MQ=255)

                                                                                                                            gccgtcagTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTCCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTtcat                          <  2:7223268/143‑1 (MQ=255)

                                                                                                                              cATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTtcatca                        <  1:1625607/150‑1 (MQ=255)

                                                                                                                               aTGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAg                       >  2:2153417/1‑150 (MQ=255)

                                                                                                                                    tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt                  >  2:4930562/1‑150 (MQ=255)

                                                                                                                                    tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt                  >  2:1387088/1‑150 (MQ=255)

                                                                                                                                    tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt                  >  1:4609879/1‑150 (MQ=255)

                                                                                                                                    tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt                  >  1:639521/1‑150 (MQ=255)

                                                                                                                                     cAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTc                 <  2:3092694/150‑1 (MQ=255)

                                                                                                                                       gcgcGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGt               <  2:3364445/150‑1 (MQ=255)

                                                                                                                                        cgcgGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTa              <  2:1042960/150‑1 (MQ=255)

                                                                                                                                        cgcgCCGTAAACCAGGAAACCGCCTGTGGTGTGCAGCACGCCCTTCCGCATGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTa              >  1:1389150/1‑150 (MQ=255)

                                                                                                                                                 aaCCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTc     >  2:6183465/1‑150 (MQ=255)

                                                                                                                                                  aCCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCGGATGTcg    >  2:5868749/1‑150 (MQ=255)

                                                                                                                                                   ccAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTcgc   >  2:6344274/1‑150 (MQ=255)

CTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCG  >  NC_002947/5100239‑5100536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`