BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,100,390	G→A	5.4%	Y324Y (TAC→TAT)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,100,390	0	G	A	5.4%	937.6 / 26.1	294	Y324Y (TAC→TAT)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (130/148); new base A (4/12); total (134/160)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 298 total aligned reads displayed.

TCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGT  >  NC_002947/5100241‑5100538

tCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTGCATGGGTCAGCGCGGCGTAAACCAgg                                                                                                                                                      <  2:1730557/150‑1 (MQ=255)

  gAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTa                                                                                                                                                    >  1:6186301/1‑150 (MQ=255)

      aGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCg                                                                                                                                                >  2:6786599/1‑150 (MQ=255)

           ggtggtGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgt                                                                                                                                           <  2:1920001/150‑1 (MQ=255)

           ggtggtGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgt                                                                                                                                           <  1:5621812/150‑1 (MQ=255)

            gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCACCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCggtg                                                                                                                                          >  2:8509426/1‑150 (MQ=255)

            gtggtgGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCtgtg                                                                                                                                          <  2:3997213/150‑1 (MQ=255)

              ggtggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTgtggt                                                                                                                                        <  1:3107374/150‑1 (MQ=255)

               gtggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTgtggtg                                                                                                                                       <  1:2064903/150‑1 (MQ=255)

                 ggCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGtgtg                                                                                                                                     <  1:3420696/150‑1 (MQ=255)

                   cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                                   <  2:4636303/150‑1 (MQ=255)

                   cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                                   >  1:5187950/1‑150 (MQ=255)

                   cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                                   <  2:2035722/150‑1 (MQ=255)

                   cgcCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgca                                                                                                                                   <  1:7293605/150‑1 (MQ=255)

                     ccGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagc                                                                                                                                 >  1:5315746/1‑150 (MQ=255)

                      cGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTgcagca                                                                                                                                >  2:1134810/1‑150 (MQ=255)

                         tGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGc                                                                                                                             >  1:7563564/1‑150 (MQ=255)

                             cAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCtt                                                                                                                         <  1:8890906/150‑1 (MQ=255)

                              aGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTc                                                                                                                        <  2:2397267/150‑1 (MQ=255)

                              aGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTc                                                                                                                        >  2:622391/1‑150 (MQ=255)

                               gCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCg                                                                                                                       >  1:8121933/1‑150 (MQ=255)

                                 gggCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGc                                                                                                                     <  2:2231354/150‑1 (MQ=255)

                                    ccGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTg                                                                                                                  >  1:911352/1‑150 (MQ=255)

                                          aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                                            <  1:2719717/150‑1 (MQ=255)

                                          aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                                            <  2:7279008/150‑1 (MQ=255)

                                          aCGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                                            <  1:6379960/150‑1 (MQ=255)

                                               ataGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGcc                                                                                                       <  1:8367496/150‑1 (MQ=255)

                                                   cTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAg                                                                                                   <  2:293407/150‑1 (MQ=255)

                                                       ggCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGtat                                                                                               <  1:1812773/150‑1 (MQ=255)

                                                        gCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGtata                                                                                              >  1:1330735/1‑150 (MQ=255)

                                                          cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTATCCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                                            <  2:7218315/150‑1 (MQ=255)

                                                          cTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATaaa                                                                                            <  2:1330735/150‑1 (MQ=255)

                                                             tcaCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGa                                                                                         <  2:8815713/148‑1 (MQ=255)

                                                             tcaCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGa                                                                                         <  2:5859896/148‑1 (MQ=255)

                                                              caCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGAt                                                                                        <  2:1636829/149‑1 (MQ=255)

                                                              caCCCAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGAt                                                                                        <  1:3863230/149‑1 (MQ=255)

                                                                 ccAGCCCACATCCGCGGTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGaa                                                                                     >  1:987769/1‑150 (MQ=255)

                                                                 ccAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGaa                                                                                     >  2:578933/1‑150 (MQ=255)

                                                                   aaCCGACGTCGGCGGTGCTCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACa                                                                                   <  1:4080134/150‑1 (MQ=255)

                                                                    aCCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAg                                                                                  <  1:2794589/150‑1 (MQ=255)

                                                                       gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                                                                               <  1:7051626/150‑1 (MQ=255)

                                                                       gACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAgcgc                                                                               <  1:2433321/150‑1 (MQ=255)

                                                                         cGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCct                                                                             <  1:7491578/150‑1 (MQ=255)

                                                                         cGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCct                                                                             <  2:7410584/150‑1 (MQ=255)

                                                                          gTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCctc                                                                            >  1:8283336/1‑150 (MQ=255)

                                                                            cggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCtt                                                                          <  2:9075126/150‑1 (MQ=255)

                                                                             ggcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTc                                                                         <  2:7960860/150‑1 (MQ=255)

                                                                              gcggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCa                                                                        <  2:6168262/150‑1 (MQ=255)

                                                                              gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                                                                               <  2:4786666/143‑2 (MQ=255)

                                                                              gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAgcgg                                                                               >  1:4786666/1‑142 (MQ=255)

                                                                              gcggTACACCAGTAAACATCGCCGTCGTGATAGTCGAAGACATATTGATGCGTCATCGAAGCATAGACGAGATAGCCGCCGGTGGTGTGAAGCACGCCCTTTGGCTTGCCGGTCGAGCCGGAGGTATAAAGGATGAACAGCGGGTCTTcc                                                                        <  2:3289124/150‑2 (MQ=255)

                                                                               cggTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAg                                                                       <  2:4562461/150‑1 (MQ=255)

                                                                                     aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                                                                 <  1:7824849/150‑1 (MQ=255)

                                                                                     aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                                                                 >  1:2026446/1‑150 (MQ=255)

                                                                                     aCCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCa                                                                 >  1:2385520/1‑150 (MQ=255)

                                                                                      ccAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAt                                                                <  1:6052555/150‑1 (MQ=255)

                                                                                      ccAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAt                                                                <  1:1100658/150‑1 (MQ=255)

                                                                                          tACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCt                                                            >  1:3494308/1‑150 (MQ=255)

                                                                                          tACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCt                                                            >  1:8054690/1‑150 (MQ=255)

                                                                                            cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                                          <  1:505496/150‑1 (MQ=255)

                                                                                            cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                                          <  1:6769843/150‑1 (MQ=255)

                                                                                            cacCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                                          <  2:6849324/150‑1 (MQ=255)

                                                                                             acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                                            <  1:772616/99‑1 (MQ=255)

                                                                                             acCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTg                                                                                                            >  2:772616/1‑99 (MQ=255)

                                                                                              ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                                        <  2:7506799/150‑1 (MQ=255)

                                                                                              ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                                        <  2:3829785/150‑1 (MQ=255)

                                                                                              ccTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTgg                                                        <  1:4457476/150‑1 (MQ=255)

                                                                                               cTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTggg                                                       >  1:1052356/1‑150 (MQ=255)

                                                                                                    ccGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCttt                                                          >  1:1725366/1‑142 (MQ=255)

                                                                                                     cGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACACATTTCTTTGGGGGCGCa                                                 >  2:5656669/1‑150 (MQ=255)

                                                                                                          ggTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATGAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGc                                            <  1:3551984/150‑1 (MQ=255)

                                                                                                             aaTCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTgg                                         >  1:4711089/1‑150 (MQ=255)

                                                                                                               tCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAg                                       <  2:3412059/150‑1 (MQ=255)

                                                                                                               tCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAGTTCTTTGGGGGCGCAGTGGCTGGAg                                       >  2:1201265/1‑150 (MQ=255)

                                                                                                               tCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCAGTTCTTTGGGGGCGCAGTGGCTGGAg                                       >  2:5921637/1‑150 (MQ=255)

                                                                                                                 gAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGc                                     <  1:7083280/150‑1 (MQ=255)

                                                                                                                  aaCACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGcc                                    >  2:4156167/1‑150 (MQ=255)

                                                                                                                  aaCACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGcc                                    >  2:4064600/1‑150 (MQ=255)

                                                                                                                   acacCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCa                                   >  2:590050/1‑150 (MQ=255)

                                                                                                                     acCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACt                                 >  2:4276987/1‑150 (MQ=255)

                                                                                                                      cccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACtt                                <  2:3601494/150‑1 (MQ=255)

                                                                                                                       ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                               >  2:811003/1‑150 (MQ=255)

                                                                                                                       ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                               >  1:2300898/1‑150 (MQ=255)

                                                                                                                       ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                               >  2:5239936/1‑150 (MQ=255)

                                                                                                                       ccGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGCAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACttt                               >  2:698747/1‑150 (MQ=255)

                                                                                                                          ttCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTtcat                            <  1:1462505/150‑1 (MQ=255)

                                                                                                                             aTGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCCGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAg                         <  2:8191974/150‑1 (MQ=255)

                                                                                                                             aTGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAg                         >  2:5373058/1‑150 (MQ=255)

                                                                                                                                  tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt                    >  2:4930562/1‑150 (MQ=255)

                                                                                                                                  tCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCtt                    >  1:4609879/1‑150 (MQ=255)

                                                                                                                                   cAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTc                   <  2:6619224/150‑1 (MQ=255)

                                                                                                                                   cAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTc                   <  2:3092706/150‑1 (MQ=255)

                                                                                                                                   cAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTc                   <  2:1986897/150‑1 (MQ=255)

                                                                                                                                      cgcgGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTa                <  1:1558239/150‑1 (MQ=255)

                                                                                                                                      cgcgGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTa                >  1:658337/1‑150 (MQ=255)

                                                                                                                                      cgcgGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTa                <  2:1042766/150‑1 (MQ=255)

                                                                                                                                         ggcgTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCa             <  1:8509426/150‑1 (MQ=255)

                                                                                                                                            gTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTCCCAGag          >  2:7688436/1‑149 (MQ=255)

                                                                                                                                             tAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATg         >  2:8308727/1‑150 (MQ=255)

                                                                                                                                                 ccAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTcgc     >  1:8801843/1‑150 (MQ=255)

TCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCCTGTGACCCAACCGACGTCGGCGGTGCACCAGTACACCTCGCCCGGACGGTAATCGAACACCCGTTCATGGGTCAGCGCGGCGTAAACCAGGTAACCGCCTGTGGTGTGCAGCACGCCCTTCGGCTTGCCGGTGGAGCCGGAGGTATAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGT  >  NC_002947/5100241‑5100538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`