BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,189,950	T→C	7.8%	L162S (TTG→TCG) ‡	PP_4567 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,189,950	0	T	C	7.8%	949.3 / 43.8	308	L162S (TTG→TCG) ‡	PP_4567	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (141/143); new base C (11/13); total (152/156)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

ATTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGCAA  >  NC_002947/5189801‑5190098

aTTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGtt                                                                                                                                                      >  2:8673373/1‑150 (MQ=255)

aTTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGtt                                                                                                                                                      >  2:4334319/1‑150 (MQ=255)

 ttGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTg                                                                                                                                                     >  2:6938560/1‑150 (MQ=255)

    cTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                                  >  2:812567/1‑150 (MQ=255)

    cTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                                  >  2:6263219/1‑150 (MQ=255)

     tGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgcc                                                                                                                                                 <  1:4763425/150‑1 (MQ=255)

       gATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgccac                                                                                                                                               >  2:3945682/1‑150 (MQ=255)

       gATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgccac                                                                                                                                               <  1:2187005/150‑1 (MQ=255)

          ttCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgccacgcc                                                                                                                                            >  2:8628109/1‑150 (MQ=255)

           tCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgccacgcca                                                                                                                                           >  2:1673498/1‑150 (MQ=255)

              gCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATcc                                                                                                                                        >  1:6860046/1‑150 (MQ=255)

               cTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATccc                                                                                                                                       >  2:5745008/1‑150 (MQ=255)

                tGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATcccc                                                                                                                                      <  2:6000257/150‑1 (MQ=255)

                   gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGaa                                                                                                                                   <  1:771921/150‑1 (MQ=38)

                   gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGaa                                                                                                                                   <  1:966414/150‑1 (MQ=38)

                   gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                   <  1:297443/150‑1 (MQ=255)

                   gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                   <  1:3148506/150‑1 (MQ=255)

                   gcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                   >  2:4981058/1‑150 (MQ=255)

                   gcCTGTGAAACAGACCAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGAGACCCTGCTCTACTACCTGCTCACCCAGCGCGACGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGaa                                                                                                                                   <  2:305643/150‑1 (MQ=37)

                    ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAag                                                                                                                                  >  1:3566246/1‑150 (MQ=255)

                    ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAag                                                                                                                                  >  1:1013119/1‑150 (MQ=255)

                     cTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAaga                                                                                                                                 <  1:7122363/150‑1 (MQ=255)

                         gAAACCGACCAAGCAGGGAAGATGTTGGCGGTCGAGGTCCCGCCGCACCGCTCGCTCGATACCCTGCTCTACGACCTGCTCCCCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTCGCGCCACGCCATCCCCCAAGAGTTc                                                                                                                             <  2:3536907/150‑1 (MQ=255)

                              cGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTa                                                                                                                        <  1:5881047/150‑1 (MQ=255)

                                 aCAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAggg                                                                                                                     <  2:4689343/150‑1 (MQ=255)

                                   aaGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGcc                                                                                                                   >  1:7553704/1‑150 (MQ=255)

                                     gCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTa                                                                                                                 <  2:5585010/150‑1 (MQ=255)

                                     gCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTa                                                                                                                 >  1:7601349/1‑150 (MQ=255)

                                        gTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGc                                                                                                              >  1:4925363/1‑150 (MQ=255)

                                        gTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGc                                                                                                              <  2:2779166/150‑1 (MQ=255)

                                           aaGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCGCCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTc                                                                                                           >  2:7810578/1‑150 (MQ=255)

                                           aaGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTc                                                                                                           >  1:5002296/1‑150 (MQ=255)

                                           aaGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTc                                                                                                           <  1:7991034/150‑1 (MQ=255)

                                              aTGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGc                                                                                                        <  1:8489883/150‑1 (MQ=255)

                                                 ttGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAg                                                                                                     <  2:6097442/150‑1 (MQ=255)

                                                   ggCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgc                                                                                                   >  1:6667749/1‑150 (MQ=255)

                                                       gTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGgcgcgc                                                                                               >  2:7896430/1‑150 (MQ=255)

                                                          gAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTGCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCgcggc                                                                                            >  1:4031850/1‑150 (MQ=255)

                                                           aGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCgcggcg                                                                                           <  1:6721704/150‑1 (MQ=255)

                                                            ggTCCCGCCGCCACGCTCGCTCGAGACCCTGCTCTACGCCCTGCTCACCCAGCGCGACGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGcggcgg                                                                                          <  2:2502113/150‑1 (MQ=255)

                                                            ggTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGcggcgg                                                                                          <  2:2242467/150‑1 (MQ=255)

                                                             gTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCggg                                                                                         >  2:1484403/1‑150 (MQ=255)

                                                             gTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCggg                                                                                         >  2:1376583/1‑150 (MQ=255)

                                                              tCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGc                                                                                        >  1:1127195/1‑150 (MQ=255)

                                                               cccGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCt                                                                                       <  1:305088/150‑1 (MQ=255)

                                                                ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCtt                                                                                      >  1:4096224/1‑150 (MQ=255)

                                                                       aaCGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAac                                                                               >  1:8961072/1‑150 (MQ=255)

                                                                        aCGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAaca                                                                              <  1:1690002/150‑1 (MQ=255)

                                                                         cgctcgctCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAacac                                                                             <  1:6263219/150‑1 (MQ=255)

                                                                            tcgctcgATACCCTGCTCTCCTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGaa                                                                          <  1:5623022/150‑1 (MQ=255)

                                                                            tcgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGaa                                                                          >  1:6003242/1‑150 (MQ=255)

                                                                              gctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGCAACACGAAca                                                                        >  2:8759470/1‑150 (MQ=255)

                                                                               ctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcag                                                                       >  2:5466191/1‑150 (MQ=255)

                                                                                tcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagc                                                                      <  1:9159622/150‑1 (MQ=255)

                                                                                tcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagc                                                                      >  1:2376369/1‑150 (MQ=255)

                                                                                   aTACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACagcagc                                                                   <  2:1076002/150‑1 (MQ=255)

                                                                                              tactacCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGAt                                                        >  2:4822365/1‑150 (MQ=255)

                                                                                                 tacCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAg                                                     <  2:183576/150‑1 (MQ=255)

                                                                                                  acCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGc                                                    <  1:3446008/150‑1 (MQ=255)

                                                                                                  acCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGc                                                    <  1:1327939/150‑1 (MQ=255)

                                                                                                   ccTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGcc                                                   >  2:3798852/1‑150 (MQ=255)

                                                                                                   ccTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGcc                                                   <  2:8296854/150‑1 (MQ=255)

                                                                                                   ccTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGcc                                                   >  1:1933537/1‑150 (MQ=255)

                                                                                                        tCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATg                                              >  1:3252937/1‑150 (MQ=255)

                                                                                                         cACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGc                                             >  1:1166114/1‑150 (MQ=255)

                                                                                                          aCCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                            >  1:7014718/1‑150 (MQ=255)

                                                                                                             cAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcag                                         >  2:8893899/1‑150 (MQ=255)

                                                                                                              aGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagc                                        >  2:7129535/1‑150 (MQ=255)

                                                                                                              aGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagc                                        >  2:7537933/1‑150 (MQ=255)

                                                                                                                cgcgAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagcag                                      >  2:657787/1‑150 (MQ=255)

                                                                                                                 gcgAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAgg                                     >  1:2556527/1‑150 (MQ=255)

                                                                                                                   gAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCt                                   >  1:8607670/1‑150 (MQ=255)

                                                                                                                          gCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGt                            <  2:6260531/150‑1 (MQ=255)

                                                                                                                          gCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGt                            <  1:5498384/150‑1 (MQ=255)

                                                                                                                           ccTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTg                           <  2:1755554/150‑1 (MQ=255)

                                                                                                                            ctgctgGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGc                          <  2:2176771/150‑1 (MQ=255)

                                                                                                                              gctgGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCa                        <  1:5745008/150‑1 (MQ=255)

                                                                                                                                  gACTTTGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg                    <  2:2956790/150‑1 (MQ=255)

                                                                                                                                  gACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg                    <  2:3687924/150‑1 (MQ=255)

                                                                                                                                     ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTTAGCGGTGCCAGGCGGtt                 >  2:8467197/1‑150 (MQ=38)

                                                                                                                                     ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                 >  1:2870275/1‑150 (MQ=38)

                                                                                                                                     ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                 >  1:2113596/1‑150 (MQ=38)

                                                                                                                                     ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                 >  2:4215992/1‑150 (MQ=38)

                                                                                                                                     ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                 >  2:8484339/1‑150 (MQ=255)

                                                                                                                                     ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGtt                 >  1:821944/1‑150 (MQ=255)

                                                                                                                                      tCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAca                                                                        <  2:7927474/94‑1 (MQ=21)

                                                                                                                                      tCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTa                >  1:4669324/1‑150 (MQ=255)

                                                                                                                                       cGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAc               <  2:6837111/150‑1 (MQ=255)

                                                                                                                                        gAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc              >  1:3658493/1‑150 (MQ=255)

                                                                                                                                        gAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc              >  2:7213775/1‑150 (MQ=255)

                                                                                                                                        gAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc              >  2:3148487/1‑150 (MQ=255)

                                                                                                                                         gggATGGCGTGGCGCAACACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCg             >  1:5357565/2‑150 (MQ=255)

                                                                                                                                           gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGaa           <  1:1732436/150‑1 (MQ=255)

                                                                                                                                                cGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCa      <  1:4334319/150‑1 (MQ=255)

                                                                                                                                                cGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCa      <  1:1376604/150‑1 (MQ=255)

                                                                                                                                                cGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCa      >  1:454993/1‑150 (MQ=255)

                                                                                                                                                cGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCa      <  2:3566268/150‑1 (MQ=255)

                                                                                                                                                cGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCa      <  1:791431/150‑1 (MQ=255)

                                                                                                                                                 gtgtTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAg     <  1:4868413/150‑1 (MQ=255)

                                                                                                                                                   gtTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGCa   <  1:812567/150‑1 (MQ=255)

ATTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAGCAA  >  NC_002947/5189801‑5190098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`