| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,309,835 | T→C | 46.2% | noncoding (2725/2902 nt) | PP_23SG ← | 23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,309,835 | 0 | T | C | 46.2% | ‑5.1 / 12.3 | 13 | noncoding (2725/2902 nt) | PP_23SG | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base C (2/4); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
CGCCTCACAGCGCTTACACACCCAACCTATCAACGTCGTAGTCTTCGACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCT > NC_002947/5309707‑5309973 | cgacTCACAGCGCTTACACACCCAACCTATCAACGTCGTAGTCTTCGACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCgg < 1:6382829/147‑1 (MQ=1) acccacCCAACCTATCAACGTCGTAGTCTTCGACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCGTAGATGCTTTCAGCGGTTATCTCTTCCGACCATAGCTACCCGGCAATGCCACTGGCGt < 2:244265/147‑1 (MQ=0) tttgACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCa > 2:3157443/4‑150 (MQ=1) ttagACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCa > 2:4577448/4‑150 (MQ=1) cccataaGGGGATTCAAGATCCCAGAGAGATCTCATCTTGAGGCAAGTTTCCCGCATAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTACGTCCACTCCGGTc > 2:720086/7‑150 (MQ=1) gaactcATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCtctcaa < 2:6834304/147‑1 (MQ=1) ctagAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCtctcaaatctcaaa < 2:6687002/147‑1 (MQ=1) gccttCAGCGGTTATCCCGTCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc < 1:627228/147‑1 (MQ=0) gccttCAGCGGTTATCCCGTCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc > 2:7953280/4‑150 (MQ=0) gccttCAGCGGTTATCCCGTCCGTATATAGCTAACCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc > 2:3337634/4‑150 (MQ=0) cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct < 1:7072608/144‑1 (MQ=0) cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct < 2:6230250/144‑1 (MQ=0) cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct < 2:6230280/144‑1 (MQ=0) | CGCCTCACAGCGCTTACACACCCAACCTATCAACGTCGTAGTCTTCGACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCT > NC_002947/5309707‑5309973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |