Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,309,878 C→A 80.1% noncoding (2682/2902 nt) PP_23SG ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,309,8780CA80.1% ‑5.5 / 14.7 43noncoding (2682/2902 nt)PP_23SG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (3/2);  major base A (15/16);  minor base T (2/5);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGC  >  NC_002947/5309750‑5310014
                                                                                                                                |                                                                                                                                        
tttgACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCa                                                                                                                     >  2:3157443/4‑150 (MQ=1)
ttagACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCa                                                                                                                     >  2:4577448/4‑150 (MQ=1)
        cccataaGGGGATTCAAGATCCCAGAGAGATCTCATCTTGAGGCAAGTTTCCCGCATAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTACGTCCACTCCGGTc                                                                                                             >  2:720086/7‑150 (MQ=1)
                                    gaactcATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCtctcaa                                                                                 <  2:6834304/147‑1 (MQ=1)
                                            ctagAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCtctcaaatctcaaa                                                                         <  2:6687002/147‑1 (MQ=1)
                                                                     gccttCAGCGGTTATCCCGTCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc                                                >  2:7953280/4‑150 (MQ=0)
                                                                     gccttCAGCGGTTATCCCGTCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc                                                <  1:627228/147‑1 (MQ=0)
                                                                     gccttCAGCGGTTATCCCGTCCGTATATAGCTAACCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc                                                >  2:3337634/4‑150 (MQ=0)
                                                                          cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct                                           <  2:6230280/144‑1 (MQ=0)
                                                                          cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct                                           <  1:7072608/144‑1 (MQ=0)
                                                                          cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct                                           <  2:6230250/144‑1 (MQ=0)
                                                                                  atcccgtCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGt                                   >  2:7348559/7‑150 (MQ=0)
                                                                                   tccctTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGtt                                  <  2:4545261/147‑1 (MQ=0)
                                                                                         ccgtataTAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACt                                               >  2:6464269/7‑131 (MQ=0)
                                                                                         ccgtataTAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACt                                               <  1:6464269/125‑1 (MQ=0)
                                                                                         ccgtataTAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAAc                            <  1:6886888/144‑1 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              >  2:7255437/7‑150 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              >  2:5946123/7‑150 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              >  2:5842217/7‑150 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              >  2:5408710/7‑150 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              <  2:3332196/144‑1 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              >  1:154373/7‑150 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              <  1:836172/144‑1 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              <  1:8223104/144‑1 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              >  1:8143102/7‑150 (MQ=0)
                                                                                                       cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc              >  1:4162928/7‑150 (MQ=0)
                                                                                                       ccagcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAcc              >  2:281772/7‑150 (MQ=0)
                                                                                                       ccagcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAcc              >  1:1469488/7‑150 (MQ=0)
                                                                                                        ctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAccg             >  1:4177393/6‑149 (MQ=0)
                                                                                                         tgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAccgc            >  1:852099/5‑148 (MQ=0)
                                                                                                         tgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAccgc            <  2:7050878/146‑3 (MQ=0)
                                                                                                         agcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAccgc            <  1:410333/146‑3 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  2:9123975/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  2:8408638/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  >  1:1676274/8‑150 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  1:2800670/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  1:3337634/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  2:7153257/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  1:6860371/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  >  1:7857267/8‑150 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  1:7953280/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc  <  2:3842006/143‑1 (MQ=0)
                                                                                                                   ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCACTTTAACTGGc  <  2:2572490/143‑1 (MQ=0)
                                                                                                                                |                                                                                                                                        
TTCGACGGCCCTTTAGGGGATTCAAGATCCCAGTGAGATCTCATCTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGC  >  NC_002947/5309750‑5310014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑