Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,309,937 T→A 72.5% noncoding (2623/2902 nt) PP_23SG ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,309,9370TA72.5% ‑4.8 / 29.8 50noncoding (2623/2902 nt)PP_23SG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (4/10);  new base A (18/18);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACA  >  NC_002947/5309794‑5310082
                                                                                                                                               |                                                                                                                                                 
ctagAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCtctcaaatctcaaa                                                                                                                                             <  2:6687002/147‑1 (MQ=1)
                         gccttCAGCGGTTATCCCGTCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc                                                                                                                    >  2:7953280/4‑150 (MQ=0)
                         gccttCAGCGGTTATCCCGTCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc                                                                                                                    <  1:627228/147‑1 (MQ=0)
                         gccttCAGCGGTTATCCCGTCCGTATATAGCTAACCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAAc                                                                                                                    >  2:3337634/4‑150 (MQ=0)
                              cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct                                                                                                               <  2:6230280/144‑1 (MQ=0)
                              cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct                                                                                                               <  2:6230250/144‑1 (MQ=0)
                              cagcacttATCCCTTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGtct                                                                                                               <  1:7072608/144‑1 (MQ=0)
                                      atcccgtCCGTATATAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGt                                                                                                       >  2:7348559/7‑150 (MQ=0)
                                       tccctTCCGCACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGtt                                                                                                      <  2:4545261/147‑1 (MQ=0)
                                             ccgtataTAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACt                                                                                                                   >  2:6464269/7‑131 (MQ=0)
                                             ccgtataTAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACt                                                                                                                   <  1:6464269/125‑1 (MQ=0)
                                             ccgtataTAGCTACCCTGCTATGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAAc                                                                                                <  1:6886888/144‑1 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  >  2:7255437/7‑150 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  >  2:5946123/7‑150 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  >  1:154373/7‑150 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  >  2:5842217/7‑150 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  >  2:5408710/7‑150 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  <  2:3332196/144‑1 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  <  1:836172/144‑1 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  <  1:8223104/144‑1 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  >  1:8143102/7‑150 (MQ=0)
                                                           cctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAcc                                                                                  >  1:4162928/7‑150 (MQ=0)
                                                           ccagcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAcc                                                                                  >  1:1469488/7‑150 (MQ=0)
                                                           ccagcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAcc                                                                                  >  2:281772/7‑150 (MQ=0)
                                                            ctgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAccg                                                                                 >  1:4177393/6‑149 (MQ=0)
                                                             tgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAccgc                                                                                >  1:852099/5‑148 (MQ=0)
                                                             tgctaTGCCGTTGGCACGACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTAccgc                                                                                <  2:7050878/146‑3 (MQ=0)
                                                             agcgaTGCCTTTGGCAAGACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTAccgc                                                                                <  1:410333/146‑3 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  2:9123975/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  2:8408638/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      >  1:1676274/8‑150 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  1:2800670/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  2:7153257/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  1:3337634/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  1:6860371/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      >  1:7857267/8‑150 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  1:7953280/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGc                                                                      <  2:3842006/143‑1 (MQ=0)
                                                                       ttggcacgACAACAGGTCCACCAGAGATACGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCACTTTAACTGGc                                                                      <  2:2572490/143‑1 (MQ=0)
                                                                                     ggtacacCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATAccc                                                        >  2:8880314/4‑150 (MQ=1)
                                                                                              gagatacGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGAcct                                               <  2:3603552/144‑2 (MQ=0)
                                                                                              gagatacGTCCATCCCGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGAAGATAGGGACCGAACTGTCTCACGACGTTCAGAACCCAACTCACGAACCGCTTTAAATGGCGAACAGCCATACCCTAGGGAcct                                               >  2:5199012/7‑149 (MQ=0)
                                                                                                      tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                       <  2:8865178/144‑1 (MQ=0)
                                                                                                      tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                       >  2:1812161/7‑150 (MQ=0)
                                                                                                      tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                       >  2:1811965/7‑150 (MQ=0)
                                                                                                          ctacGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTAAGCCCCa                                   <  2:7921664/147‑1 (MQ=1)
                                                                                                                     tcttACTAGGAGCAGCCCATCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGACTAACGAAGTTCAAAACACAGATCTCGTACCACTATAAATGGAGAACAGCCATACCCTTGGGACCGGATTCAGCCCCAGGATGtgatga                        >  2:6164102/4‑150 (MQ=1)
                                                                                                                                   gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt          <  1:5103618/147‑1 (MQ=1)
                                                                                                                                   gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt          <  2:8387243/147‑1 (MQ=1)
                                                                                                                                   gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt          <  2:4924099/147‑1 (MQ=1)
                                                                                                                                   gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt          <  2:4924346/147‑1 (MQ=1)
                                                                                                                                           cacatctcaaaCGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCACGCGTACCCCTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAaca  <  2:1039809/147‑1 (MQ=0)
                                                                                                                                               |                                                                                                                                                 
CTTGAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGAACATAGCTACCCGGCAATGCCACTGGCGTGACAACCGGAACACCAGAGGTTCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACA  >  NC_002947/5309794‑5310082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑