Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,310,037 G→T 35.9% noncoding (2523/2902 nt) PP_23SG ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,310,0370GT35.9% ‑3.3 / 25.8 28noncoding (2523/2902 nt)PP_23SG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (3/15);  new base T (4/6);  total (7/21)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

TCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC  >  NC_002947/5309896‑5310174
                                                                                                                                             |                                                                                                                                         
tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                                                                                                                   <  2:8865178/144‑1 (MQ=0)
tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                                                                                                                   >  2:1812161/7‑150 (MQ=0)
tccatcccGGTCCTCTCGTACTAGGGACAGATCCTATCAATATTCCTACACCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAACTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGc                                                                                                                                   >  2:1811965/7‑150 (MQ=0)
    ctacGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTAAGCCCCa                                                                                                                               <  2:7921664/147‑1 (MQ=1)
               tcttACTAGGAGCAGCCCATCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGACTAACGAAGTTCAAAACACAGATCTCGTACCACTATAAATGGAGAACAGCCATACCCTTGGGACCGGATTCAGCCCCAGGATGtgatga                                                                                                                    >  2:6164102/4‑150 (MQ=1)
                             gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                      <  1:5103618/147‑1 (MQ=1)
                             gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                      <  2:8387243/147‑1 (MQ=1)
                             gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                      <  2:4924346/147‑1 (MQ=1)
                             gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                      <  2:4924099/147‑1 (MQ=1)
                                     cacatctcaaaCGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCACGCGTACCCCTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAaca                                                                                              <  2:1039809/147‑1 (MQ=0)
                                             aatcGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTc                                                                                      >  1:3309900/4‑150 (MQ=1)
                                                cgcccACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGat                                                                                   <  1:3486470/147‑1 (MQ=1)
                                                cgcccACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGat                                                                                   <  1:2846902/147‑1 (MQ=1)
                                                cgcccACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGat                                                                                   <  1:2153106/147‑1 (MQ=1)
                                                                                              caacTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCtttt                                     <  2:3673770/147‑1 (MQ=1)
                                                                                              caacTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCtttt                                     <  2:3674680/147‑1 (MQ=1)
                                                                                                  tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGTGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                 >  2:4705111/4‑150 (MQ=1)
                                                                                                  tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                 <  2:235258/147‑1 (MQ=1)
                                                                                                  tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                 <  2:235276/147‑1 (MQ=1)
                                                                                                  tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                 >  1:6163179/4‑150 (MQ=1)
                                                                                                         ccgcTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGc                          <  2:5864205/147‑1 (MQ=1)
                                                                                                         ccgcTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGc                          >  1:1746696/4‑150 (MQ=1)
                                                                                                         ccgcTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGc                          <  1:988398/147‑1 (MQ=1)
                                                                                                              ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGTGATgg                     <  1:1238696/144‑1 (MQ=1)
                                                                                                              ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGTGATgg                     <  1:1238828/144‑1 (MQ=1)
                                                                                                              ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATgg                     <  2:3759565/144‑1 (MQ=1)
                                                                                                              ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATgg                     <  2:7640347/144‑1 (MQ=1)
                                                                                                                                 acgcTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAaccac  <  2:1955606/147‑1 (MQ=1)
                                                                                                                                             |                                                                                                                                         
TCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC  >  NC_002947/5309896‑5310174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑