Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,310,062 C→T 32.4% noncoding (2498/2902 nt) PP_23SG ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,310,0620CT32.4% ‑2.1 / 29.0 37noncoding (2498/2902 nt)PP_23SG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (4/21);  new base T (5/7);  total (9/28)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

GCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCT  >  NC_002947/5309925‑5310202
                                                                                                                                         |                                                                                                                                            
gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                                                  <  2:4924346/147‑1 (MQ=1)
gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                                                  <  2:8387243/147‑1 (MQ=1)
gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                                                  <  1:5103618/147‑1 (MQ=1)
gctccTCTCAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGt                                                                                                                                  <  2:4924099/147‑1 (MQ=1)
        cacatctcaaaCGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCACGCGTACCCCTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAaca                                                                                                                          <  2:1039809/147‑1 (MQ=0)
                aatcGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTc                                                                                                                  >  1:3309900/4‑150 (MQ=1)
                   cgcccACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGat                                                                                                               <  1:2153106/147‑1 (MQ=1)
                   cgcccACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGat                                                                                                               <  1:2846902/147‑1 (MQ=1)
                   cgcccACGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGat                                                                                                               <  1:3486470/147‑1 (MQ=1)
                                                                 caacTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCtttt                                                                 <  2:3674680/147‑1 (MQ=1)
                                                                 caacTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCtttt                                                                 <  2:3673770/147‑1 (MQ=1)
                                                                     tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGTGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                             >  2:4705111/4‑150 (MQ=1)
                                                                     tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                             >  1:6163179/4‑150 (MQ=1)
                                                                     tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                             <  2:235276/147‑1 (MQ=1)
                                                                     tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                             <  2:235258/147‑1 (MQ=1)
                                                                            ccgcTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGc                                                      <  2:5864205/147‑1 (MQ=1)
                                                                            ccgcTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGc                                                      >  1:1746696/4‑150 (MQ=1)
                                                                            ccgcTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGc                                                      <  1:988398/147‑1 (MQ=1)
                                                                                 ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGTGATgg                                                 <  1:1238828/144‑1 (MQ=1)
                                                                                 ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGTGATgg                                                 <  1:1238696/144‑1 (MQ=1)
                                                                                 ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATgg                                                 <  2:7640347/144‑1 (MQ=1)
                                                                                 ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATgg                                                 <  2:3759565/144‑1 (MQ=1)
                                                                                                    acgcTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAaccac                              <  2:1955606/147‑1 (MQ=1)
                                                                                                              cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                    >  2:5153013/4‑150 (MQ=1)
                                                                                                              cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                    >  1:5077992/4‑150 (MQ=1)
                                                                                                               cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                   <  2:2081096/144‑1 (MQ=1)
                                                                                                               cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                   <  1:748232/144‑1 (MQ=1)
                                                                                                                gactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAg                  >  2:9040615/6‑150 (MQ=1)
                                                                                                                 gcgtCAGCCCCAGGATGGGCTGAGCCGCCAGCGAGGTGCCACCCCCCGCCGTCGAGATGAACTCTTGGGCGGCATCCGCCTGTTATCCCCGGAGTACCTTTTAGCCGATGAGCGCGGGCCCTTCCATACAGAACCACCGGATCACTAAGa                 <  1:7420054/147‑1 (MQ=1)
                                                                                                                 actacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAgc                 <  1:2790585/146‑2 (MQ=1)
                                                                                                                 actacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAgc                 <  2:8007705/146‑2 (MQ=1)
                                                                                                                  ctccAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGAc                <  2:8438695/147‑1 (MQ=1)
                                                                                                                          cctggATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc        >  2:2519133/4‑150 (MQ=1)
                                                                                                                          cctggATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc        <  1:2536936/147‑1 (MQ=1)
                                                                                                                          cctggATGTGATGAGCCGACAGCGAGGTGCCACACACCGCCGTCGATATGAACTCTTGGGCGGTTTCTGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc        <  2:5649341/147‑1 (MQ=1)
                                                                                                                              gttgcgatgaGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGAGTGTTGGGGGTGATCAGCCTGTTATCCCCGGCGTAGCTTTTATCCGTTGAGCGATGGGCCTTGCACGCGGGACCACCGGATGACTATGTCCGTCTTTCGtct    >  2:6580598/6‑148 (MQ=0)
                                                                                                                                tgcgatgaGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGtctct  <  1:5657304/147‑5 (MQ=1)
                                                                                                                                         |                                                                                                                                            
GCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCT  >  NC_002947/5309925‑5310202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑