Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,310,130 A→C 54.8% noncoding (2430/2902 nt) PP_23SG ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,310,1300AC54.8% ‑5.7 / 33.0 33noncoding (2430/2902 nt)PP_23SG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (2/5);  major base C (5/10);  minor base G (2/9);  total (9/24)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

CAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTC  >  NC_002947/5309990‑5310263
                                                                                                                                            |                                                                                                                                     
caacTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCtttt                                                                                                                              <  2:3674680/147‑1 (MQ=1)
caacTCACGTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCtttt                                                                                                                              <  2:3673770/147‑1 (MQ=1)
    tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGTGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                                                                                          >  2:4705111/4‑150 (MQ=1)
    tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                                                                                          <  2:235276/147‑1 (MQ=1)
    tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                                                                                          <  2:235258/147‑1 (MQ=1)
    tcacgTACCGCTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATcc                                                                                                                          >  1:6163179/4‑150 (MQ=1)
           ccgcTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGc                                                                                                                   <  2:5864205/147‑1 (MQ=1)
           ccgcTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGc                                                                                                                   >  1:1746696/4‑150 (MQ=1)
           ccgcTTTAAATGGCGAACAGCCATACCCTTGGGACCTGCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGc                                                                                                                   <  1:988398/147‑1 (MQ=1)
                ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGTGATgg                                                                                                              <  1:1238696/144‑1 (MQ=1)
                ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGTGATgg                                                                                                              <  1:1238828/144‑1 (MQ=1)
                ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATgg                                                                                                              <  2:7640347/144‑1 (MQ=1)
                ttaatgggCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATgg                                                                                                              <  2:3759565/144‑1 (MQ=1)
                                   acgcTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAaccac                                                                                           <  2:1955606/147‑1 (MQ=1)
                                             cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                                                                                 >  2:5153013/4‑150 (MQ=1)
                                             cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                                                                                 >  1:5077992/4‑150 (MQ=1)
                                              cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                                                                                <  1:748232/144‑1 (MQ=1)
                                              cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                                                                                <  2:2081096/144‑1 (MQ=1)
                                               gactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAg                                                                               >  2:9040615/6‑150 (MQ=1)
                                                gcgtCAGCCCCAGGATGGGCTGAGCCGCCAGCGAGGTGCCACCCCCCGCCGTCGAGATGAACTCTTGGGCGGCATCCGCCTGTTATCCCCGGAGTACCTTTTAGCCGATGAGCGCGGGCCCTTCCATACAGAACCACCGGATCACTAAGa                                                                              <  1:7420054/147‑1 (MQ=1)
                                                actacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAgc                                                                              <  1:2790585/146‑2 (MQ=1)
                                                actacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAgc                                                                              <  2:8007705/146‑2 (MQ=1)
                                                 ctccAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGAc                                                                             <  2:8438695/147‑1 (MQ=1)
                                                         cctggATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc                                                                     <  1:2536936/147‑1 (MQ=1)
                                                         cctggATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc                                                                     >  2:2519133/4‑150 (MQ=1)
                                                         cctggATGTGATGAGCCGACAGCGAGGTGCCACACACCGCCGTCGATATGAACTCTTGGGCGGTTTCTGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc                                                                     <  2:5649341/147‑1 (MQ=1)
                                                             gttgcgatgaGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGAGTGTTGGGGGTGATCAGCCTGTTATCCCCGGCGTAGCTTTTATCCGTTGAGCGATGGGCCTTGCACGCGGGACCACCGGATGACTATGTCCGTCTTTCGtct                                                                 >  2:6580598/6‑148 (MQ=0)
                                                               tgcgatgaGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGtctct                                                               <  1:5657304/147‑5 (MQ=1)
                                                                      agtcGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGAc                                                        <  2:4584520/147‑1 (MQ=1)
                                                                      agtcGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGAc                                                        <  2:5724984/147‑1 (MQ=1)
                                                                      agtcGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGAc                                                        <  1:1060911/147‑1 (MQ=1)
                                                                                                          gatctctTGGGCGGTATCAGCCTGTTATCCCCGGAGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTta                    >  2:1919709/4‑150 (MQ=1)
                                                                                                                            agacTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTc  <  1:7651898/147‑1 (MQ=1)
                                                                                                                                            |                                                                                                                                     
CAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTC  >  NC_002947/5309990‑5310263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑