Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,310,165 A→G 61.6% noncoding (2395/2902 nt) PP_23SG ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,310,1650AG61.6% ‑5.3 / 22.5 26noncoding (2395/2902 nt)PP_23SG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (3/7);  new base G (5/11);  total (8/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTG  >  NC_002947/5310025‑5310306
                                                                                                                                            |                                                                                                                                             
acgcTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAaccac                                                                                                                                      <  2:1955606/147‑1 (MQ=1)
          cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                                                                                                                            >  1:5077992/4‑150 (MQ=1)
          cctgCTCCAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTa                                                                                                                            >  2:5153013/4‑150 (MQ=1)
           cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                                                                                                                           <  1:748232/144‑1 (MQ=1)
           cgactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTaa                                                                                                                           <  2:2081096/144‑1 (MQ=1)
            gactacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAg                                                                                                                          >  2:9040615/6‑150 (MQ=1)
             gcgtCAGCCCCAGGATGGGCTGAGCCGCCAGCGAGGTGCCACCCCCCGCCGTCGAGATGAACTCTTGGGCGGCATCCGCCTGTTATCCCCGGAGTACCTTTTAGCCGATGAGCGCGGGCCCTTCCATACAGAACCACCGGATCACTAAGa                                                                                                                         <  1:7420054/147‑1 (MQ=1)
             actacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAgc                                                                                                                         <  1:2790585/146‑2 (MQ=1)
             actacAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAgc                                                                                                                         <  2:8007705/146‑2 (MQ=1)
              ctccAGCCCCAGGATGCGATGAGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGAc                                                                                                                        <  2:8438695/147‑1 (MQ=1)
                      cctggATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc                                                                                                                <  1:2536936/147‑1 (MQ=1)
                      cctggATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc                                                                                                                >  2:2519133/4‑150 (MQ=1)
                      cctggATGTGATGAGCCGACAGCGAGGTGCCACACACCGCCGTCGATATGAACTCTTGGGCGGTTTCTGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTc                                                                                                                <  2:5649341/147‑1 (MQ=1)
                          gttgcgatgaGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGAGTGTTGGGGGTGATCAGCCTGTTATCCCCGGCGTAGCTTTTATCCGTTGAGCGATGGGCCTTGCACGCGGGACCACCGGATGACTATGTCCGTCTTTCGtct                                                                                                            >  2:6580598/6‑148 (MQ=0)
                            tgcgatgaGTCGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGtctct                                                                                                          <  1:5657304/147‑5 (MQ=1)
                                   agtcGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGAc                                                                                                   <  2:4584520/147‑1 (MQ=1)
                                   agtcGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGAc                                                                                                   <  2:5724984/147‑1 (MQ=1)
                                   agtcGACATCGAGGTGCCAAACAACCCCGTCGATATGGACTCTTGGGGGTCATCAGCCTGTTATCCCCGGCGTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGAc                                                                                                   <  1:1060911/147‑1 (MQ=1)
                                                                       gatctctTGGGCGGTATCAGCCTGTTATCCCCGGAGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTta                                                               >  2:1919709/4‑150 (MQ=1)
                                                                                         agacTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTc                                             <  1:7651898/147‑1 (MQ=1)
                                                                                                       ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCTGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc                               <  2:8243483/147‑1 (MQ=0)
                                                                                                       ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCt                                   <  2:4385741/143‑1 (MQ=0)
                                                                                                       ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCt                                   >  1:4385741/4‑146 (MQ=0)
                                                                                                         aggaCCTTTTAGCCGTTGCGCGACGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAgcgc                             <  2:5133511/147‑1 (MQ=0)
                                                                                                                          gaccGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATAATCTACGTCCGATTTCCGACCGGTCTGAGCGCTCCTTCGTACTCCTCCg            >  2:8216657/4‑150 (MQ=0)
                                                                                                                                    ccgtCCAGACAGCACCCCCGGATCACGAAGACCTACTTTCGTACCGGCTCGACGTGTATGTCTCGCAGTCAAGCGCGCGTTTGCCTTCATACTCTCCGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGGTACTCttgg  <  2:3505395/147‑3 (MQ=1)
                                                                                                                                            |                                                                                                                                             
ACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTG  >  NC_002947/5310025‑5310306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑