Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,310,237	T→A	69.8%	noncoding (2323/2902 nt)	PP_23SG ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,310,237	0	T	A	69.8%	‑6.2 / 4.7	16	noncoding (2323/2902 nt)	PP_23SG	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base A (7/4);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACC  >  NC_002947/5310096‑5310337                                                                                                                                              |                                                                                                     gatctctTGGGCGGTATCAGCCTGTTATCCCCGGAGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTta                                                                                              >  2:1919709/4‑150 (MQ=1)                   agacTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTc                                                                            <  1:7651898/147‑1 (MQ=1)                                 ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCTGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAgc                                                              <  2:8243483/147‑1 (MQ=0)                                 ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCt                                                                  >  1:4385741/4‑146 (MQ=0)                                 ggcgTACCTTTTATCCGTTGAGCGATGGCCCTTCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCt                                                                  <  2:4385741/143‑1 (MQ=0)                                   aggaCCTTTTAGCCGTTGCGCGACGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAgcgc                                                            <  2:5133511/147‑1 (MQ=0)                                                    gaccGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATAATCTACGTCCGATTTCCGACCGGTCTGAGCGCTCCTTCGTACTCCTCCg                                           >  2:8216657/4‑150 (MQ=0)                                                              ccgtCCAGACAGCACCCCCGGATCACGAAGACCTACTTTCGTACCGGCTCGACGTGTATGTCTCGCAGTCAAGCGCGCGTTTGCCTTCATACTCTCCGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGGTACTCttgg                                 <  2:3505395/147‑3 (MQ=1)                                                                                        tatgACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTAc       <  1:1892727/147‑1 (MQ=0)                                                                                        tatgACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTAc       <  1:3003291/147‑1 (MQ=0)                                                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc  >  1:1111024/4‑150 (MQ=0)                                                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc  >  1:3207860/4‑150 (MQ=0)                                                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc  >  1:6293344/4‑150 (MQ=0)                                                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc  >  2:2530519/4‑150 (MQ=0)                                                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc  >  2:827206/4‑150 (MQ=0)                                                                                             ccgaCTTTCGTCTCTGCTCGACTTGTCAGTCTTGCAGTCAGGCAGGCTTATGCCATTGCACTCGACGAACGATTTCCGACCGTTCTGAGCCTACCATCGCGCGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACccacc  >  2:833160/4‑150 (MQ=0)                                                                                                                                              |                                                                                                     GAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAAGACCTACTTTCGTACCTGCTCGACGTGTTTGTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTCCGTTACTCTTTGGGAGGAGACCGCCCCAGTCAAACTACCCACC  >  NC_002947/5310096‑5310337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑