BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,313,362	G→A	46.7%	noncoding (1290/1522 nt)	PP_16SG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,313,362	0	G	A	46.7%	‑4.4 / 37.4	30	noncoding (1290/1522 nt)	PP_16SG	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (5/11); new base A (9/5); total (14/16)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCAC  >  NC_002947/5313236‑5313508

acgtCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGtttt                                                                                                                              <  1:6660546/147‑1 (MQ=1)

      ggcagaaCCAACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGAt                                                                                                                       <  2:6622353/145‑1 (MQ=1)

      ggcagaaCCAACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGAt                                                                                                                       <  2:1392014/145‑1 (MQ=1)

             ccaaCTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GATATTAGCTca                                                                                                                >  2:6004546/4‑149 (MQ=1)

             ccaaCTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTca                                                                                                                >  1:4844414/4‑149 (MQ=1)

             ccaaCTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTca                                                                                                                <  1:6120392/147‑2 (MQ=1)

             ccaaCTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTca                                                                                                                >  1:6471878/4‑149 (MQ=1)

                          tgagACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCtt                                                                                                   <  1:8477859/147‑1 (MQ=1)

                                                                    cggcATGCTGATCCGCGATTACTAGCGATTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCa                                                         <  2:2040187/147‑1 (MQ=0)

                                                                        atgcTGATCCGCGATTACTAGCGATTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAggtc                                                     <  1:1518647/147‑3 (MQ=0)

                                                                        atgcTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCg                                                    >  1:8378354/4‑150 (MQ=0)

                                                                              atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                              <  1:7371031/147‑1 (MQ=0)

                                                                              atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                              <  1:5638953/147‑1 (MQ=0)

                                                                              atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                              <  1:1969153/147‑1 (MQ=0)

                                                                                                  ccaaCTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCAt                          <  1:8356669/147‑1 (MQ=0)

                                                                                                  ccaaCTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCAt                          <  2:771242/147‑1 (MQ=0)

                                                                                                          atgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCAcc                   <  1:21772/143‑1 (MQ=0)

                                                                                                          atgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCAcc                   <  1:2016106/143‑1 (MQ=0)

                                                                                                           tgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCt                  >  2:1976148/7‑150 (MQ=0)

                                                                                                             taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                >  2:6350059/5‑150 (MQ=0)

                                                                                                             taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                >  2:6350083/5‑150 (MQ=0)

                                                                                                             taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                >  2:6873893/5‑150 (MQ=0)

                                                                                                             taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                <  2:8834504/146‑1 (MQ=0)

                                                                                                             cactCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTtcc               <  2:3005396/147‑1 (MQ=0)

                                                                                                             aaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                >  2:6873868/5‑150 (MQ=0)

                                                                                                              aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc               >  2:2977507/4‑150 (MQ=0)

                                                                                                              aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc               >  2:2819442/4‑150 (MQ=0)

                                                                                                              aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc               >  2:7438776/4‑150 (MQ=0)

                                                                                                              aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc               >  2:7438887/4‑150 (MQ=0)

                                                                                                                          gagtGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTATCAc  >  1:8029712/4‑150 (MQ=0)

ACTTCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCAC  >  NC_002947/5313236‑5313508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`