BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,313,399	C→G	58.6%	noncoding (1253/1522 nt)	PP_16SG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,313,399	0	C	G	58.6%	‑6.5 / 24.4	26	noncoding (1253/1522 nt)	PP_16SG	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base C (0/2); major base G (9/5); minor base A (2/8); total (11/15)
Fisher's exact test for biased strand distribution p-value = 4.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTC  >  NC_002947/5313262‑5313518

tgagACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCtt                                                                                                             <  1:8477859/147‑1 (MQ=1)

                                          cggcATGCTGATCCGCGATTACTAGCGATTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCa                                                                   <  2:2040187/147‑1 (MQ=0)

                                              atgcTGATCCGCGATTACTAGCGATTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAggtc                                                               <  1:1518647/147‑3 (MQ=0)

                                              atgcTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCg                                                              >  1:8378354/4‑150 (MQ=0)

                                                    atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                                        <  1:1969153/147‑1 (MQ=0)

                                                    atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                                        <  1:5638953/147‑1 (MQ=0)

                                                    atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                                        <  1:7371031/147‑1 (MQ=0)

                                                                        ccaaCTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCAt                                    <  2:771242/147‑1 (MQ=0)

                                                                        ccaaCTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCAt                                    <  1:8356669/147‑1 (MQ=0)

                                                                                atgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCAcc                             <  1:2016106/143‑1 (MQ=0)

                                                                                atgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCAcc                             <  1:21772/143‑1 (MQ=0)

                                                                                 tgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCt                            >  2:1976148/7‑150 (MQ=0)

                                                                                   taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          <  2:8834504/146‑1 (MQ=0)

                                                                                   taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6873893/5‑150 (MQ=0)

                                                                                   taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6350083/5‑150 (MQ=0)

                                                                                   taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6350059/5‑150 (MQ=0)

                                                                                   cactCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTtcc                         <  2:3005396/147‑1 (MQ=0)

                                                                                   aaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6873868/5‑150 (MQ=0)

                                                                                    aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:2977507/4‑150 (MQ=0)

                                                                                    aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:2819442/4‑150 (MQ=0)

                                                                                    aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:7438776/4‑150 (MQ=0)

                                                                                    aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:7438887/4‑150 (MQ=0)

                                                                                                gagtGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTATCAc            >  1:8029712/4‑150 (MQ=0)

                                                                                                    gcaaTCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTATCACTGGc        <  2:2183364/147‑1 (MQ=0)

                                                                                                    gcaaTCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTATCACTGGc        <  2:7737934/147‑1 (MQ=0)

                                                                                                           ggccTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGtctc  <  1:6101721/147‑1 (MQ=1)

TGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTC  >  NC_002947/5313262‑5313518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`