BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,313,455	G→C	43.4%	noncoding (1197/1522 nt)	PP_16SG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,313,455	0	G	C	43.4%	‑6.9 / 26.3	23	noncoding (1197/1522 nt)	PP_16SG	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (9/4); new base C (2/8); total (11/12)
Fisher's exact test for biased strand distribution p-value = 3.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTC  >  NC_002947/5313308‑5313518

atgcTGATCCGCGATTACTAGCGATTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAggtc                                                               <  1:1518647/147‑3 (MQ=0)

atgcTGATCCGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCg                                                              >  1:8378354/4‑150 (MQ=0)

      atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                                        <  1:1969153/147‑1 (MQ=0)

      atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                                        <  1:5638953/147‑1 (MQ=0)

      atccgcgATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAggg                                                        <  1:7371031/147‑1 (MQ=0)

                          ccaaCTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCAt                                    <  2:771242/147‑1 (MQ=0)

                          ccaaCTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCAt                                    <  1:8356669/147‑1 (MQ=0)

                                  atgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCAcc                             <  1:2016106/143‑1 (MQ=0)

                                  atgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCAcc                             <  1:21772/143‑1 (MQ=0)

                                   tgtaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCt                            >  2:1976148/7‑150 (MQ=0)

                                     taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          <  2:8834504/146‑1 (MQ=0)

                                     taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6873893/5‑150 (MQ=0)

                                     taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6350083/5‑150 (MQ=0)

                                     taggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6350059/5‑150 (MQ=0)

                                     cactCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTtcc                         <  2:3005396/147‑1 (MQ=0)

                                     aaggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtc                          >  2:6873868/5‑150 (MQ=0)

                                      aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:2977507/4‑150 (MQ=0)

                                      aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:2819442/4‑150 (MQ=0)

                                      aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:7438776/4‑150 (MQ=0)

                                      aggcGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATTTGCTCGACCTCGCG‑GTTTTGCTGCCCTTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTtcc                         >  2:7438887/4‑150 (MQ=0)

                                                  gagtGCAATCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTATCAc            >  1:8029712/4‑150 (MQ=0)

                                                      gcaaTCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTATCACTGGc        <  2:2183364/147‑1 (MQ=0)

                                                      gcaaTCCGAACTGAGATGGCTTTTG‑GAGATTAGCTCACACTCGCGTGCTT‑GCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCAGCTTATCACTGGc        <  2:7737934/147‑1 (MQ=0)

                                                             ggccTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGtctc  <  1:6101721/147‑1 (MQ=1)

ATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACGATCGGTTTTGTGAGATTAGCTCCACCTCGCG‑GCTTGGCAACCCTCTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTC  >  NC_002947/5313308‑5313518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`