Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,348,932 T→G 6.4% Y128* (TAT→TAG)  PP_4703 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,348,9320TG 6.4% 826.7 / 6.5 277Y128* (TAT→TAG) PP_4703hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (203/47);  new base G (2/23);  total (207/70)
Fisher's exact test for biased strand distribution p-value = 1.90e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

CGTGAAGACCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGCCGAGCGCC  >  NC_002947/5348783‑5349080
                                                                                                                                                      |                                                                                                                                                     
cGTGAAGACCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTAt                                                                                                                                                       <  1:5078754/150‑1 (MQ=255)
cGTGAAGACCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTAt                                                                                                                                                       >  2:3261807/1‑150 (MQ=255)
 gTGAAGACCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATg                                                                                                                                                      >  1:3598626/1‑150 (MQ=255)
  tGAAGACCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCGTATGc                                                                                                                                                     >  1:3837495/1‑150 (MQ=255)
       aCCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTGATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑a                                                                                                                                               <  1:2683794/150‑1 (MQ=255)
       aCCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑a                                                                                                                                               <  2:8292023/150‑1 (MQ=255)
       aCCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTAGTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑a                                                                                                                                               <  1:4048483/150‑1 (MQ=255)
           gCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGa                                                                                                                                           >  1:7870095/1‑150 (MQ=255)
            cTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGAc                                                                                                                                          >  1:6370937/1‑150 (MQ=255)
            cTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGAc                                                                                                                                          >  1:2005199/1‑150 (MQ=255)
                 ccGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGa                                                                                                                                     >  2:8530617/1‑150 (MQ=255)
                    ggggccccgcACCAGCCAGATAAGTCCCGGCACCACCTCGGGCGCCTGGGGCTTTGCTGTGTGGCTTATCTAAAACGTCCCAACT‑TTCACCCGGCGGGGCCCGGCCACGCCACCCGTGGGCCGCCGTTCTGCGGCC‑AGCGACCCGGATCt                                                                                                                                  <  2:3983928/144‑1 (MQ=255)
                     ttGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTg                                                                                                                                 >  2:5862389/1‑150 (MQ=255)
                     ttGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTg                                                                                                                                 >  2:7330109/1‑150 (MQ=255)
                       gcgcCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGGCCTTATGCGGCC‑AGCGACCCGGATCTGtt                                                                                                                               <  2:1830645/150‑1 (MQ=255)
                       gcgcCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGtt                                                                                                                               >  1:5045794/1‑150 (MQ=255)
                       gcgcCCCACCCGCCTGTTCTGTTCCGGCACCCCCGTCGGCGCCAGGGGCGATGCGTTGTGGCTTATCCACAACGTGCGCAATTTGCA‑CCTGCAGGTCACGGGAACGGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGtt                                                                                                                               <  2:7068548/150‑1 (MQ=255)
                         gccgcACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGa                                                                                                                             >  2:6349322/1‑150 (MQ=255)
                         gccgcACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGa                                                                                                                             >  1:993682/1‑150 (MQ=255)
                                 agccagTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTg                                                                                                                     >  2:8578863/1‑150 (MQ=255)
                                  gccagTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGc                                                                                                                    >  2:7135381/1‑150 (MQ=255)
                                  gccagTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGc                                                                                                                    >  2:2218778/1‑150 (MQ=255)
                                   ccagTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCa                                                                                                                   >  1:8638318/1‑150 (MQ=255)
                                   ccagTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCa                                                                                                                   >  1:5835382/1‑150 (MQ=255)
                                   ccagTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCa                                                                                                                   >  1:4226346/1‑150 (MQ=255)
                                     agTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATg                                                                                                                 >  1:5414939/1‑150 (MQ=255)
                                     agTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATg                                                                                                                 >  1:8858237/1‑150 (MQ=255)
                                       ttCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTa                                                                                                               >  2:5133139/1‑150 (MQ=255)
                                          aTTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCg                                                                                                            >  1:7488759/1‑150 (MQ=255)
                                           tttCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGCTCTGTTGACCTGGGTGCATGTAGCgg                                                                                                           >  2:8850470/1‑150 (MQ=255)
                                           tttCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCgg                                                                                                           >  2:3801269/1‑150 (MQ=255)
                                           tttCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCgg                                                                                                           >  2:9076556/1‑150 (MQ=255)
                                           tttCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCgg                                                                                                           >  1:3097308/1‑150 (MQ=255)
                                           tttCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCgg                                                                                                           >  1:2366443/1‑150 (MQ=255)
                                           tttCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCgg                                                                                                           >  1:2308031/1‑150 (MQ=255)
                                            ttCCGGCACCACCTCCGCCGCCAGGGGCTTTCCCGAGTGCATTATCTACAAAGTCCCCAATATTCA‑CCCGCAGCACACCGCAACCGCACCCCTTGGCC‑CACATTTGCCGCCAAGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGa                                                                                                          <  2:5597523/150‑1 (MQ=255)
                                                 gCACCACGGTCGGCGCCACGGGCTATGCGGTGTGGCTTATCGAAAAAGTGCCCACTATCCA‑CCTGCTGGCCACGGCCACTGCACCCGAGGGCCCCCCTTAGGCGCCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCa                                                                                                     <  2:6511393/150‑1 (MQ=255)
                                                 gCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCa                                                                                                     >  1:4829349/1‑150 (MQ=255)
                                                   accaccTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGc                                                                                                   >  1:7606922/1‑150 (MQ=255)
                                                    ccaccGTCGGCGCCCGGGGCCAGGCGGTGGGGCTGATCCACACCGGGCCCACTATCCA‑CCGCCGGGACCCCGGCCCGCCACCCGCGGGCCCCCCTTAGGCGCCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGcc                                                                                                  <  2:2538733/150‑1 (MQ=255)
                                                     caccTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCg                                                                                                 >  1:4174258/1‑150 (MQ=255)
                                                     caccTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCg                                                                                                 >  1:5745937/1‑150 (MQ=255)
                                                      accTTCGGCGCCACGGGCGATGCCGAGTGGTTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGt                                                                                                >  2:5534499/1‑150 (MQ=255)
                                                        cTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGttt                                                                                              >  2:6725497/1‑150 (MQ=255)
                                                         ttCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTc                                                                                             >  2:7577602/1‑150 (MQ=255)
                                                          tCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTcc                                                                                            >  2:8368880/1‑150 (MQ=255)
                                                           cGGCGCCACGGGCGATGCCGAGTGGCTGACCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCt                                                                                           <  2:7488759/150‑1 (MQ=255)
                                                             gcgcCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCGCCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCg                                                                                         >  2:2413770/1‑150 (MQ=255)
                                                               gcCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCt                                                                                       >  1:764353/1‑150 (MQ=255)
                                                                ccACGGGCGTTGCCTAGTGGCTGCTCGACAACGTGCGCACTATTCC‑CCTGCAGGTCACCGGCACTGCACCCGAGGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTg                                                                                      <  2:5948045/150‑1 (MQ=255)
                                                                             cGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTa                                                                         >  1:2446683/1‑150 (MQ=255)
                                                                              gAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTAc                                                                        >  2:7448467/1‑150 (MQ=255)
                                                                              gAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTAc                                                                        >  1:6874827/1‑150 (MQ=255)
                                                                                   gCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCaa                                                                   >  2:1294483/1‑150 (MQ=255)
                                                                                     tGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGTCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAAcc                                                                 >  2:5530946/1‑150 (MQ=255)
                                                                                     tGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAAcc                                                                 >  1:1680612/1‑150 (MQ=255)
                                                                                     tGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAAcc                                                                 >  2:2045277/1‑150 (MQ=255)
                                                                                     tGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAAcc                                                                 >  1:4270280/1‑150 (MQ=255)
                                                                                     tGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAAcc                                                                 >  1:1978268/1‑150 (MQ=255)
                                                                                        tCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCAc                                                              >  1:3231353/1‑150 (MQ=255)
                                                                                          gaaaatgTGCGCACTATCCA‑CCTGCTGGTCACCGCCACTGCACCCGATGGCCGCCCTTTTGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGc                                                            <  2:3195194/144‑1 (MQ=255)
                                                                                          gACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGc                                                            >  1:8049576/1‑150 (MQ=255)
                                                                                          gACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGc                                                            >  1:6214101/1‑150 (MQ=255)
                                                                                          gACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGc                                                            >  2:5859040/1‑150 (MQ=255)
                                                                                            cAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCt                                                          >  1:8569446/1‑150 (MQ=255)
                                                                                             aatgTGCCCACTTTCCA‑CCGGCTGGTCACCGGCCCTCCACCCGAGGGCCGCCCTTAGGCGGCC‑CGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTg                                                         <  2:7658544/147‑1 (MQ=255)
                                                                                                     cACTATTCA‑CCTGCCGGTCACGGGCACTGCACCCGTTGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACgcgc                                                 <  2:5319871/150‑1 (MQ=255)
                                                                                                             ccccTGCCGGCCCCCGGCACGGCACCCGCGGGCCGCCCTTAGGCGCCC‑CGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAg                                          <  2:7792562/148‑1 (MQ=255)
                                                                                                             a‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAgg                                         >  2:4911157/1‑150 (MQ=255)
                                                                                                             a‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAgg                                         >  2:8056930/1‑150 (MQ=255)
                                                                                                              cccTGCTGGTCACCGGCACTCCCCCCGAGGGCCCCCCTTCGGCGCCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAgg                                         <  1:6417807/149‑1 (MQ=255)
                                                                                                                cTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGAt                                       >  1:6704023/1‑150 (MQ=255)
                                                                                                                 tGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATg                                      >  1:280408/1‑150 (MQ=255)
                                                                                                                  ggaGGTCACCGGCAGTGCTCCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGAGCTGGGTGCTTGTTGCGGTGGTGAGGCGTTTCCTCGCTGCGCACCTGCGTTTCCGCAACCCTCGCCTGCGACGCGCCGAGCAGGATGc                                     >  2:6059752/3‑150 (MQ=255)
                                                                                                                  gccggccACCGGCACTGCACCCGCGGGCCGCCCTTCTGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGc                                     <  2:7181128/144‑1 (MQ=255)
                                                                                                                  gCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGc                                     >  2:3572313/1‑150 (MQ=255)
                                                                                                                       gcccgggCACTGCACCGGTGGGCCGCCATTTGGCGGCA‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTAtt                                <  2:6885483/145‑1 (MQ=255)
                                                                                                                         aCCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTAc                              >  1:6970349/1‑150 (MQ=255)
                                                                                                                         aCCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTAc                              >  2:3389610/1‑150 (MQ=255)
                                                                                                                          ccGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACa                             >  1:1118982/1‑150 (MQ=255)
                                                                                                                          ccGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACa                             >  2:6428754/1‑150 (MQ=255)
                                                                                                                          ccGGCACTGCACCCGAGGGCCGCCCTTTTGCGCCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACa                             <  2:4285288/150‑1 (MQ=255)
                                                                                                                            ggCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAAc                           >  2:6581332/1‑150 (MQ=255)
                                                                                                                            gccACTGCCCCCGAGGGCCGCCATTAGGCGCCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAAc                           <  1:7160029/148‑1 (MQ=255)
                                                                                                                                    aCCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGc                   >  1:7756640/1‑150 (MQ=255)
                                                                                                                                     cccgtgggcggcCCTTTGGCCCCC‑CGCGACCCGGATCTGCTGCCCTGGGCGCCCGCAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGcc                  <  2:4161755/144‑1 (MQ=255)
                                                                                                                                     cccGTTGGCCGCCCTTTGGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGcc                  <  2:4354996/150‑1 (MQ=255)
                                                                                                                                      ccgtgggCCGCCCTTCGGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGccc                 <  1:8183786/145‑1 (MQ=255)
                                                                                                                                      ccgtgggCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGccc                 <  2:1285217/145‑1 (MQ=255)
                                                                                                                                      ccGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGTAGGATGCCTATTACAACGAAATCGccc                 >  2:1526980/1‑150 (MQ=255)
                                                                                                                                      ccGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGccc                 >  1:7355669/1‑150 (MQ=255)
                                                                                                                                       ggtgggCCGCCTTTTTGCGCCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCt                <  1:8663470/146‑1 (MQ=255)
                                                                                                                                         ctGGCCGCCCTTAGGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCTGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGa              <  1:1411281/149‑1 (MQ=255)
                                                                                                                                             ccgccCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGc          >  1:7046347/1‑150 (MQ=255)
                                                                                                                                             ccgccCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGc          <  2:7521121/150‑1 (MQ=255)
                                                                                                                                              cgccCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGcc         >  1:4884803/1‑150 (MQ=255)
                                                                                                                                              cgccCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGcc         >  2:5751342/1‑150 (MQ=255)
                                                                                                                                              cgccCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGcc         >  1:2603456/1‑150 (MQ=255)
                                                                                                                                                cccTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGCCGa       >  2:4509006/1‑150 (MQ=255)
                                                                                                                                                     aTGCGGCC‑AGCGACCCTGATCTGTTGACCTGGGTGCTTGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGCCGAGCGcc  <  2:701650/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGTGAAGACCTGCTCGGCCGCTTGCGCCGCACCAGCCAGTTCATTTCCGGCACCACCTTCGGCGCCACGGGCGATGCCGAGTGGCTGATCGACAAAGTGCGCACTATTCA‑CCTGCAGGTCACCGGCACTGCACCCGATGGCCGCCCTTATGCGGCC‑AGCGACCCGGATCTGTTGACCTGGGTGCATGTAGCGGAGGTCAGCCGTTTCCTCGCTGCCCACCTGCGTTACCGCAACCCACGCCTGCCACGCGCCGAGCAGGATGCCTATTACAACGAAATCGCCCTGATCGCCGAGCGCC  >  NC_002947/5348783‑5349080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑