Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,355,907 C→G 5.3% S60S (TCG→TCC) ‡ pnp ← polyribonucleotide nucleotidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,355,9070CG 5.3% 1017.3 / 32.1 300S60S (TCG→TCC) ‡pnppolyribonucleotide nucleotidyltransferase
Reads supporting (aligned to +/- strand):  ref base C (148/136);  new base G (12/4);  total (160/140)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 308 total aligned reads displayed.

TCGTTCATGAAGCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGATTGACC  >  NC_002947/5355758‑5356056
                                                                                                                                                     |                                                                                                                                                     
  gTTCATGAAGCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGa                                                                                                                                                     >  2:5321805/1‑150 (MQ=255)
    tCATGAAGCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACa                                                                                                                                                   >  1:3796374/1‑150 (MQ=255)
       tGAAGCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCg                                                                                                                                                >  2:7601710/1‑150 (MQ=255)
        gAAGCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCgg                                                                                                                                               <  1:1184685/150‑1 (MQ=255)
           gCCTTCCGGTAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGgaa                                                                                                                                            <  2:5776999/150‑1 (MQ=255)
           gCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGgaa                                                                                                                                            <  2:2423741/150‑1 (MQ=255)
           gCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGgaa                                                                                                                                            <  2:2122197/150‑1 (MQ=255)
           gCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGgaa                                                                                                                                            >  2:6432316/1‑150 (MQ=255)
              atcaacgAAAAGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGTAAGTCTTTTCCTGATAGTTGACGGTCAGCGGgaagaa                                                                                                                                         >  1:6044059/7‑150 (MQ=255)
              atcaacgAAAAGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGTAAGTCTTTTCCTGATAGTTGACGGTCAGCGGgaagaa                                                                                                                                         >  2:2869845/7‑150 (MQ=255)
               tCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGaaa                                                                                                                                        <  1:5603894/150‑1 (MQ=255)
                ccGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAAc                                                                                                                                       <  2:490171/150‑1 (MQ=255)
                ccGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTCGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAAc                                                                                                                                       <  2:3418214/150‑1 (MQ=255)
                 aacgAAAAGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGTAAGTCTTTTCCTGATAGTTGACGGTCAGCGGGAAGaaatc                                                                                                                                      <  2:3746368/147‑3 (MQ=255)
                  gggAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAAccc                                                                                                                                     <  2:3744321/150‑1 (MQ=255)
                   ggAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCt                                                                                                                                    >  2:6613888/1‑150 (MQ=255)
                   cgAAAAGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGTAAGTCTTTTCCTGATAGTTGACGGTCAGCGGGAAGAAATCCt                                                                                                                                    >  2:945219/2‑150 (MQ=255)
                    gAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCtt                                                                                                                                   >  2:6729345/1‑150 (MQ=255)
                    gAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCtt                                                                                                                                   <  1:3341256/150‑1 (MQ=255)
                    gAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCtt                                                                                                                                   <  1:7917106/150‑1 (MQ=255)
                    gAAAAGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGTAAGTCTTTTCCTGATAGTTGACGGTCAGCGGGAAGAAATCCtg                                                                                                                                   >  1:6340477/1‑149 (MQ=255)
                     aaaaGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGGAAGTCTTTTCCTGATAGTTGACGGTCAGCGGGAAGAAATCCtga                                                                                                                                  >  2:7163901/4‑148 (MQ=255)
                      aCAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGc                                                                                                                                 <  2:2740444/150‑1 (MQ=255)
                      aCAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGc                                                                                                                                 <  1:6168060/150‑1 (MQ=255)
                      aCAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGc                                                                                                                                 <  1:3878880/150‑1 (MQ=255)
                       aaGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGTAAGTCTTTTCCTGATAGTTGACGGTCAGCGGGAAGAAATCCtgacc                                                                                                                                >  2:7831803/2‑146 (MQ=255)
                        aGCGGGCGGATCGGACGGTCGATCAGGCGCGAAACCAGAGTTTCGTTTTCGCTTGGACGACCTTCGCGCTTGAAGAAACCACCTGGGATCTTGCCAGCTGCGTAAGTCTTTTCCTGATAGTTGACGGTCAGCGGGAAGAAATCCtgaccc                                                                                                                               <  1:8777337/150‑6 (MQ=255)
                         gCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTg                                                                                                                              >  2:6137488/1‑150 (MQ=255)
                          cggacggaTCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTgg                                                                                                                             >  1:583349/1‑150 (MQ=255)
                              cggaTCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCa                                                                                                                         <  1:7255422/150‑1 (MQ=255)
                                  tCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCt                                                                                                                     >  1:6139457/1‑150 (MQ=255)
                                     gACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGtt                                                                                                                  <  2:3811697/150‑1 (MQ=255)
                                      aCGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGttt                                                                                                                 <  1:671353/150‑1 (MQ=255)
                                       cGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTg                                                                                                                >  1:7347758/1‑150 (MQ=255)
                                        ggTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTgg                                                                                                               >  2:2520882/1‑150 (MQ=255)
                                            gATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGcc                                                                                                           >  1:8288983/1‑150 (MQ=255)
                                            gATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGcc                                                                                                           >  1:1821366/1‑150 (MQ=255)
                                              tCAGGCGCGAGGTCATCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTa                                                                                                         <  2:5452514/150‑1 (MQ=255)
                                              tCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTa                                                                                                         >  2:4308395/1‑150 (MQ=255)
                                              tCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTa                                                                                                         <  1:7983753/150‑1 (MQ=255)
                                               cAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTAc                                                                                                        <  1:5206439/150‑1 (MQ=255)
                                                 ggCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACca                                                                                                      <  1:3487493/150‑1 (MQ=255)
                                                   cgcgAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACcaca                                                                                                    <  2:8416016/150‑1 (MQ=255)
                                                   cgcgAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACcaca                                                                                                    <  2:5139482/150‑1 (MQ=255)
                                                   cgcgAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACcaca                                                                                                    <  2:8058596/150‑1 (MQ=255)
                                                    gcgAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAg                                                                                                   >  1:3082324/1‑150 (MQ=255)
                                                    gcgAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAg                                                                                                   >  2:8618063/1‑150 (MQ=255)
                                                     cgAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGt                                                                                                  <  2:1718481/150‑1 (MQ=255)
                                                        ggTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCAc                                                                                               >  1:8894330/1‑150 (MQ=255)
                                                        ggTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCAc                                                                                               <  2:5222318/150‑1 (MQ=255)
                                                        ggTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCAc                                                                                               >  1:6584378/1‑150 (MQ=255)
                                                                tctcTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACgg                                                                                       >  1:4992296/1‑150 (MQ=255)
                                                                 ctctTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGt                                                                                      <  2:2675911/150‑1 (MQ=255)
                                                                    tttCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGAc                                                                                   >  1:4884137/1‑150 (MQ=255)
                                                                    tttCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGAc                                                                                   >  2:4471398/1‑150 (MQ=255)
                                                                    tttCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGAc                                                                                   >  1:7547792/1‑150 (MQ=255)
                                                                    tttCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGAc                                                                                   >  2:6972377/1‑150 (MQ=255)
                                                                       ctcGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGATACGCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGAcgtg                                                                                >  2:2246122/1‑149 (MQ=255)
                                                                        tcGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGAcgtcg                                                                               >  1:8534715/1‑150 (MQ=255)
                                                                          ggAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGtt                                                                             >  1:5706675/1‑150 (MQ=255)
                                                                           gAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTg                                                                            >  1:783177/1‑150 (MQ=255)
                                                                              ggACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCg                                                                         <  1:2724736/150‑1 (MQ=255)
                                                                               gACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAGGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGa                                                                        >  1:6800796/1‑150 (MQ=255)
                                                                               gACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGa                                                                        >  2:7425177/1‑150 (MQ=255)
                                                                                   gCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGt                                                                    >  1:6348996/1‑150 (MQ=255)
                                                                                     cTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTaa                                                                  >  2:6504307/1‑150 (MQ=255)
                                                                                      ttCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAAc                                                                 >  2:5626629/1‑150 (MQ=255)
                                                                                      ttCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAAc                                                                 >  1:1082045/1‑150 (MQ=255)
                                                                                          cGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGc                                                             <  1:7798110/150‑1 (MQ=255)
                                                                                           gCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCa                                                            >  1:1738041/1‑150 (MQ=255)
                                                                                           gCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCa                                                            >  2:6130523/1‑150 (MQ=255)
                                                                                           gCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCa                                                            >  1:6484657/1‑150 (MQ=255)
                                                                                             ttGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACg                                                          >  2:1261344/1‑150 (MQ=255)
                                                                                             ttGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACg                                                          >  1:9062673/1‑150 (MQ=255)
                                                                                             ttGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACg                                                          >  2:7565020/1‑150 (MQ=255)
                                                                                                 agaagCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGgcgc                                                      >  2:3009906/1‑150 (MQ=255)
                                                                                                         ccTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGcc                                              >  1:2102610/1‑150 (MQ=255)
                                                                                                         ccTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGcc                                              <  1:4903940/150‑1 (MQ=255)
                                                                                                          cTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCt                                             <  1:4511862/150‑1 (MQ=255)
                                                                                                              gATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACg                                         <  1:8385192/150‑1 (MQ=255)
                                                                                                                tCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACggg                                       <  2:1581239/150‑1 (MQ=255)
                                                                                                                 cTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGc                                      >  1:3990139/1‑150 (MQ=255)
                                                                                                                   tGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCaa                                    <  1:419389/150‑1 (MQ=255)
                                                                                                                        gcggcgTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACgg                               >  2:599627/1‑150 (MQ=255)
                                                                                                                         cggcgTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGc                              >  1:7097562/1‑150 (MQ=255)
                                                                                                                           gcgTAGGTCTTTTCCTGGTAGTGAACCGACCGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGccc                            <  2:201290/150‑1 (MQ=255)
                                                                                                                               acgTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGttt                        >  2:8118475/3‑150 (MQ=255)
                                                                                                                                  tCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCga                     <  2:363705/150‑1 (MQ=255)
                                                                                                                                      ttCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGt                 >  2:6275190/1‑150 (MQ=255)
                                                                                                                                         cTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGAc              >  2:3213048/1‑150 (MQ=255)
                                                                                                                                          tGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACg             >  2:7525777/1‑150 (MQ=255)
                                                                                                                                            gTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGt           >  2:6076297/1‑150 (MQ=255)
                                                                                                                                            gTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGt           >  1:1538846/1‑150 (MQ=255)
                                                                                                                                               gTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGa        <  1:2969729/150‑1 (MQ=255)
                                                                                                                                               gTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGa        <  1:8180622/150‑1 (MQ=255)
                                                                                                                                                 gAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGAtt      <  1:6892953/150‑1 (MQ=255)
                                                                                                                                                 gAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGAtt      <  1:2520882/150‑1 (MQ=255)
                                                                                                                                                    ccGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGATTGAc   <  2:1189798/150‑1 (MQ=255)
                                                                                                                                                    ccGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGATTGAc   <  1:1069711/150‑1 (MQ=255)
                                                                                                                                                     cGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGATTGAcc  <  2:898680/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGTTCATGAAGCCTTCCGGGAACAGCGGACGGATCGGACGGTCGATCAGGCGCGAGGTCAGCGTCTCTTTCTCGGAAGGACGGCCTTCACGCTTGAAGAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGTCGATTGACC  >  NC_002947/5355758‑5356056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑