Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,367,722 G→A 14.6% G268G (GGC→GGT)  ftsH ← integral membrane ATP‑dependent zinc metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,367,7220GA14.6% 810.7 / 109.1 323G268G (GGC→GGT) ftsHintegral membrane ATP‑dependent zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base G (150/125);  new base A (17/30);  total (168/155)
Fisher's exact test for biased strand distribution p-value = 2.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

GCGGCCGGGGCGCAGCAGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAA  >  NC_002947/5367575‑5367871
                                                                                                                                                   |                                                                                                                                                     
  ggCCGGGGCGCAGCAGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCgg                                                                                                                                                   <  2:683863/150‑1 (MQ=255)
   gCCGGGGCGCAGCAGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGACATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGgc                                                                                                                                                  <  2:766382/150‑1 (MQ=255)
     cGGGGCGCAGCAGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGgcgc                                                                                                                                                <  1:5543360/150‑1 (MQ=255)
     cGGGGCGCAGCAGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGgcgc                                                                                                                                                <  2:3147619/150‑1 (MQ=255)
       gggCGCAGCAGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCa                                                                                                                                              <  2:55660/150‑1 (MQ=255)
           gcagcaGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGAt                                                                                                                                          >  1:3952159/1‑150 (MQ=255)
             agcagcGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATgg                                                                                                                                        >  1:7766877/1‑150 (MQ=255)
               cagcGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCg                                                                                                                                      >  2:489976/1‑150 (MQ=255)
                       ggTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACgg                                                                                                                              >  1:1456584/1‑150 (MQ=255)
                           gAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTc                                                                                                                          >  2:5806632/1‑150 (MQ=255)
                              cacaTCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGAt                                                                                                                       <  2:4284965/150‑1 (MQ=255)
                                 aTCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTtc                                                                                                                    >  2:258920/1‑150 (MQ=255)
                                   ccGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTtcgt                                                                                                                  <  2:2551396/150‑1 (MQ=255)
                                    cGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTtcgtc                                                                                                                 <  2:8319769/150‑1 (MQ=255)
                                     ggACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGTGACCAACGGCGTCGATTtcgtcg                                                                                                                <  2:8633603/150‑1 (MQ=255)
                                     ggACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTtcgtcg                                                                                                                >  1:6682265/1‑150 (MQ=255)
                                     ggACGGTTGGTGACGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTtcgtcg                                                                                                                >  1:5859545/1‑150 (MQ=255)
                                      gACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGa                                                                                                               <  1:2775577/150‑1 (MQ=255)
                                      gACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGa                                                                                                               <  1:1176951/150‑1 (MQ=255)
                                         ggTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGa                                                                                                            >  1:4767809/1‑150 (MQ=255)
                                            tggtggCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAga                                                                                                         >  2:7216889/1‑150 (MQ=255)
                                             ggtggCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAgat                                                                                                        <  2:1017322/150‑1 (MQ=255)
                                               tggCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAgatga                                                                                                      >  2:2773261/1‑150 (MQ=255)
                                               tggCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAgatga                                                                                                      >  2:7365155/1‑150 (MQ=255)
                                                       atgacgatgaTGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACggg                                                                                              <  2:641165/150‑1 (MQ=255)
                                                           cgatgaTGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCATCTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGt                                                                                          <  1:3154066/150‑1 (MQ=255)
                                                                   ccATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTg                                                                                  <  1:921732/150‑1 (MQ=255)
                                                                   ccATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTg                                                                                  <  2:4838822/150‑1 (MQ=255)
                                                                   ccATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTg                                                                                  >  1:6719044/1‑150 (MQ=255)
                                                                   ccATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTg                                                                                  <  2:449769/150‑1 (MQ=255)
                                                                   ccATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTg                                                                                  <  2:6476753/150‑1 (MQ=255)
                                                                   ccATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTg                                                                                  <  1:2565956/150‑1 (MQ=255)
                                                                    catcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTgg                                                                                 >  1:6965751/1‑150 (MQ=255)
                                                                    catcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTgg                                                                                 >  2:2512617/1‑150 (MQ=255)
                                                                     atcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGc                                                                                <  1:4306246/150‑1 (MQ=255)
                                                                     atcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGc                                                                                <  1:5812884/150‑1 (MQ=255)
                                                                     atcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGc                                                                                >  2:4062971/1‑150 (MQ=255)
                                                                      tcattcatCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGcc                                                                               >  1:5125916/1‑150 (MQ=255)
                                                                             tctcGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGa                                                                        >  2:3329315/1‑150 (MQ=255)
                                                                             tctcGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGa                                                                        >  2:4587353/1‑150 (MQ=255)
                                                                              ctcGCAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCGCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGaa                                                                       <  1:5156975/150‑1 (MQ=255)
                                                                               tcGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTtcgtcg                                                                                                                <  1:7974091/108‑1 (MQ=255)
                                                                                 gAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACAt                                                                    >  1:7854910/1‑150 (MQ=255)
                                                                                  aaGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATg                                                                   <  2:1822420/150‑1 (MQ=255)
                                                                                       gTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTcgcg                                                              >  2:8950196/1‑150 (MQ=255)
                                                                                        tCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCgcgc                                                             >  1:5880434/1‑150 (MQ=255)
                                                                                         ccATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGca                                                            >  2:4022173/1‑150 (MQ=255)
                                                                                         ccATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTGCTTGGCCTGCTCGAACATGTCGCGca                                                            >  2:6178623/1‑150 (MQ=255)
                                                                                            tttcgaccaGAAGCTGGTTCAGCGTCTGTTCGCGTTCGTCGTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGgacgc                                                         >  2:1130395/6‑148 (MQ=255)
                                                                                            tttcgaccaGAAGCTGGTTCAGCGTCTGTTCGCGTTCGTCGTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGgacgc                                                         <  2:1887866/145‑3 (MQ=255)
                                                                                            tctcCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCacac                                                         >  1:5360115/1‑150 (MQ=255)
                                                                                              tcCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACgg                                                       >  1:7894732/1‑150 (MQ=255)
                                                                                                gaccaGAAGCTGGTTCAGCGTCTGTTCGCGTTCGTCGTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCt                                                     >  1:3369710/2‑150 (MQ=255)
                                                                                                caccaGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCt                                                     <  2:881764/150‑1 (MQ=255)
                                                                                                caccaGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCt                                                     >  2:8203081/1‑150 (MQ=255)
                                                                                                caccaGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCt                                                     >  2:3872353/1‑150 (MQ=255)
                                                                                                caccaGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCt                                                     <  2:2097256/150‑1 (MQ=255)
                                                                                                 accaGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTg                                                    >  1:2978429/1‑150 (MQ=255)
                                                                                                 accaGAAGCTGGTTCAGCGTCTGTTCGCGTTCGTCGTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCtt                                                    <  1:8172697/150‑2 (MQ=255)
                                                                                                    agaagcTGGTTCAGCGTCTGTTCGCGTTCGTCGTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCa                                                 <  1:2087284/145‑1 (MQ=255)
                                                                                                    agaagcTGGTTCAGCGTCTGTTCGCGTTCGTCGTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCa                                                 <  1:894885/145‑1 (MQ=255)
                                                                                                           ggTTCAGCGTCTGTTCGCGTTCGTCGTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAacac                                          <  1:7374781/150‑1 (MQ=255)
                                                                                                             ttGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACAccc                                        >  2:6716230/1‑150 (MQ=255)
                                                                                                              tgtgggTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCa                                       <  1:877279/147‑1 (MQ=255)
                                                                                                               gAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATg                                                                   <  2:6229187/121‑1 (MQ=255)
                                                                                                               gAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCAc                                      >  1:7271662/1‑150 (MQ=255)
                                                                                                                   gTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAAc                                  >  2:8101876/1‑150 (MQ=255)
                                                                                                                        cTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTc                             >  2:234839/1‑150 (MQ=255)
                                                                                                                        cTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTc                             >  2:7751316/1‑150 (MQ=255)
                                                                                                                         tcgcgttcgtcgTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTca                            <  1:4977589/144‑2 (MQ=255)
                                                                                                                         tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTcc                            <  1:2364449/150‑1 (MQ=255)
                                                                                                                         tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTcc                            >  2:7544271/1‑150 (MQ=255)
                                                                                                                          cgcgttcgtcgTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTcaa                           <  2:151495/145‑3 (MQ=255)
                                                                                                                          cACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCa                           >  2:3518805/1‑150 (MQ=255)
                                                                                                                           aCGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGacac                                          >  2:3734631/1‑134 (MQ=255)
                                                                                                                           aCGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGacac                                          <  1:3734631/134‑1 (MQ=255)
                                                                                                                            cgttcgtcgTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTCAAca                         >  2:5111731/4‑149 (MQ=255)
                                                                                                                              ttcgtcgTTGCCGCCGCCTAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTCAACaaa                       <  1:8234012/149‑1 (MQ=255)
                                                                                                                              ttcgtcgTTGCCGCCGCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTCAACaaa                       <  1:8843065/149‑1 (MQ=255)
                                                                                                                              cTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGaa                       <  1:489976/150‑1 (MQ=255)
                                                                                                                               tcgtcgTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGACACCGACACCCAc                                      >  1:4104222/1‑134 (MQ=255)
                                                                                                                                 gtcgtGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTc                    <  1:1165141/150‑1 (MQ=255)
                                                                                                                                 gtcgtGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTc                    <  2:1724007/150‑1 (MQ=255)
                                                                                                                                 gtcgtGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTc                    <  1:4035321/150‑1 (MQ=255)
                                                                                                                                  tcgtGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACAccc                                        <  1:6458305/129‑1 (MQ=255)
                                                                                                                                  tcgtGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTcc                   >  2:7950255/1‑150 (MQ=255)
                                                                                                                                   cgttgccgccgCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTCAACAAAGTcag                  >  1:5346754/6‑148 (MQ=255)
                                                                                                                                    gtGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGa                 >  1:3619231/1‑150 (MQ=255)
                                                                                                                                     tGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGaa                >  1:9079644/1‑150 (MQ=255)
                                                                                                                                      tgccgccgCCAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTCAACAAAGTCAGAAc               <  1:3009004/148‑1 (MQ=255)
                                                                                                                                      gACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAAc               >  1:215886/1‑150 (MQ=255)
                                                                                                                                      gACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAAc               >  1:8421204/1‑150 (MQ=255)
                                                                                                                                         cgccgcCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCgg            <  2:5393530/150‑1 (MQ=255)
                                                                                                                                         cgccgcCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCgg            >  2:2179012/1‑150 (MQ=255)
                                                                                                                                          gccgccCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACGCACGAACATTTCCACGAAGTCCGAACCGGa           >  1:1271330/1‑150 (MQ=255)
                                                                                                                                          gccgccAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTCAACAAAGTCAGAACCAGa           >  1:7499282/1‑150 (MQ=255)
                                                                                                                                          gccgccAAGACCGGCACCACGGTGGCGACCGACGGCGTCGATTTCGTCGATGAAGATGATGCAAGGCGCATTCTTCTTGGCCTGTTCGAACATGTCACGGACGCGGCTTGCACCAACACCGACAAACATTTCAACAAAGTCAGAACCAGa           <  1:1504619/150‑1 (MQ=255)
                                                                                                                                             gccCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGAGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAAt        >  2:5674176/1‑150 (MQ=255)
                                                                                                                                                cATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGt     >  1:1039410/1‑150 (MQ=255)
                                                                                                                                                  tGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTga   <  1:3809524/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCGGCCGGGGCGCAGCAGCGCAGGGTCGAGCACATCCGGACGGTTGGTGGCGGCGATGACGATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAA  >  NC_002947/5367575‑5367871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑