BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,128	C→A	6.8%	V469V (GTG→GTT)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,128	0	C	A	6.8%	1216.6 / 51.7	364	V469V (GTG→GTT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base C (180/159); new base A (15/10); total (195/169)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 366 total aligned reads displayed.

GCGTCACGCACCATACGCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGT  >  NC_002947/5377979‑5378277

     aCGCACCATACGCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCAcgcc                                                                                                                                                  >  2:359765/1‑150 (MQ=255)

           cATACGCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCgg                                                                                                                                            >  2:7815221/1‑150 (MQ=255)

                gCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTg                                                                                                                                       >  1:6556087/1‑150 (MQ=255)

                gCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTg                                                                                                                                       >  1:4335978/1‑150 (MQ=255)

                gCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTg                                                                                                                                       <  2:5643864/150‑1 (MQ=255)

                gCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTg                                                                                                                                       >  1:8005347/1‑150 (MQ=255)

                    gATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCgg                                                                                                                                   <  2:7874370/150‑1 (MQ=255)

                    gATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCgg                                                                                                                                   <  2:5808938/150‑1 (MQ=255)

                    gATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCgg                                                                                                                                   >  2:108910/1‑150 (MQ=255)

                    gATCTCTTCGTCCGACAGGCCGGAGGTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCgg                                                                                                                                   >  2:6274656/1‑150 (MQ=255)

                       ctctTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGTTGGCGGAAt                                                                                                                                <  1:4861905/150‑1 (MQ=255)

                       ctctTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAAt                                                                                                                                <  1:4268584/150‑1 (MQ=255)

                         ctTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGt                                                                                                                              >  1:3499574/1‑150 (MQ=255)

                          ttCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTc                                                                                                                             <  1:4452983/150‑1 (MQ=255)

                          ttCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTc                                                                                                                             >  2:2044838/1‑150 (MQ=255)

                              tCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGcc                                                                                                                         >  1:7797313/1‑150 (MQ=255)

                              tCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGcc                                                                                                                         >  1:414669/1‑150 (MQ=255)

                               ccGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCa                                                                                                                        <  1:2366065/150‑1 (MQ=255)

                                cGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAg                                                                                                                       >  1:2226383/1‑150 (MQ=255)

                                 gACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAgg                                                                                                                      >  2:7331589/1‑150 (MQ=255)

                                  aCAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGt                                                                                                                     >  1:3078909/1‑150 (MQ=255)

                                  aCAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGt                                                                                                                     >  1:4112190/1‑150 (MQ=255)

                                      gCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGaa                                                                                                                 >  2:4179367/1‑150 (MQ=255)

                                      gCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGaa                                                                                                                 <  2:4447669/150‑1 (MQ=255)

                                      gCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGaa                                                                                                                 >  1:2327343/1‑150 (MQ=255)

                                      gCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGaa                                                                                                                 <  1:2000071/150‑1 (MQ=255)

                                       ccGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAAt                                                                                                                                <  1:399265/134‑1 (MQ=255)

                                          gAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTg                                                                                                             <  1:258175/150‑1 (MQ=255)

                                             ttGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGccc                                                                                                          <  1:8395026/150‑1 (MQ=255)

                                              tGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCa                                                                                                         <  2:4766204/150‑1 (MQ=255)

                                              tGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCa                                                                                                         >  1:6483472/1‑150 (MQ=255)

                                                 ccTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGTCAGGTCGAACTTGCCCAGCg                                                                                                      >  2:6523355/1‑150 (MQ=255)

                                                   ttGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGAc                                                                                                    >  1:1358611/1‑150 (MQ=255)

                                                     gATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACtt                                                                                                  <  1:5319027/150‑1 (MQ=255)

                                                        cACGATCGACTGAGTCTTGCCGGTGGCGTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCAGTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGtt                                                                                               >  1:5917111/1‑150 (MQ=255)

                                                        cACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGtt                                                                                               <  2:325423/150‑1 (MQ=255)

                                                         aCGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTc                                                                                              <  2:3056116/150‑1 (MQ=255)

                                                           gATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTg                                                                                            >  2:6134834/1‑150 (MQ=255)

                                                           gATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTg                                                                                            >  2:2205409/1‑150 (MQ=255)

                                                           gATCGACTGAGTCTTGCCCGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGACTGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTg                                                                                            >  2:83208/1‑150 (MQ=255)

                                                                 cTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGc                                                                                      >  2:8460319/1‑150 (MQ=255)

                                                                 cTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGc                                                                                      <  1:3231959/150‑1 (MQ=255)

                                                                  tGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGcc                                                                                     >  2:5955771/1‑150 (MQ=255)

                                                                  tGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGcc                                                                                     >  1:7053004/1‑150 (MQ=255)

                                                                   gttccTTGCCTGTGCCCTTGTCCTTTGCGGACACGTTCACGATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGcca                                                                                    >  1:8979727/5‑149 (MQ=255)

                                                                      ccTTGCCTGTGCCCTTGTCCTTTGCGGACACGTTCACGATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGACTGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGccattt                                                                                 >  2:8607063/2‑146 (MQ=255)

                                                                      ccTTGCCTGTGCCCTTGTCCTTTGCGGACACGTTCACGATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGccattt                                                                                 >  2:2344506/2‑146 (MQ=255)

                                                                        ttGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTa                                                                               <  2:8998354/150‑1 (MQ=255)

                                                                            cGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCt                                                                           <  2:9060000/150‑1 (MQ=255)

                                                                             ggTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTCCTGCGCAGCCTGCTTACGCTc                                                                          <  2:499761/150‑1 (MQ=255)

                                                                             ggTGGACTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGTTTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCATGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTc                                                                          <  2:1618905/150‑1 (MQ=255)

                                                                                  ccTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCtt                                                                     >  2:5256026/1‑150 (MQ=255)

                                                                                   cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                    >  1:6067863/1‑150 (MQ=255)

                                                                                   cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                    <  1:8705160/150‑1 (MQ=255)

                                                                                   cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                    >  1:471090/1‑150 (MQ=255)

                                                                                   cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                    >  1:6218822/1‑150 (MQ=255)

                                                                                    ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                   <  1:7566204/150‑1 (MQ=255)

                                                                                    ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                   <  1:73041/150‑1 (MQ=255)

                                                                                       tCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgcagc                                                                >  1:8660026/1‑150 (MQ=255)

                                                                                       tCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgcagc                                                                >  2:8078717/1‑150 (MQ=255)

                                                                                          ttCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACg                                                             >  2:3805746/1‑150 (MQ=255)

                                                                                             gCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGa                                                          >  1:1767029/1‑150 (MQ=255)

                                                                                              cACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGaa                                                         <  1:3780333/150‑1 (MQ=255)

                                                                                               aCCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAAt                                                        <  2:3929901/150‑1 (MQ=255)

                                                                                               aCCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTAGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAAt                                                        <  1:8090793/150‑1 (MQ=255)

                                                                                                   aCGTTCACGATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTc                                                    >  2:877529/1‑150 (MQ=255)

                                                                                                     gttcacgATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCAc                                                  >  2:9091893/7‑150 (MQ=255)

                                                                                                      tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGTACGTGAATGGTCACg                                                 <  1:3444146/150‑1 (MQ=255)

                                                                                                           gATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccga                                            >  1:5110960/1‑147 (MQ=255)

                                                                                                              gCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTg                                         >  2:7933233/1‑150 (MQ=255)

                                                                                                              gCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTg                                         <  2:7073360/150‑1 (MQ=255)

                                                                                                               ccGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTgg                                        >  1:254479/1‑150 (MQ=255)

                                                                                                                 gTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                      <  2:4028484/150‑1 (MQ=255)

                                                                                                                 gTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                      >  2:2846036/1‑150 (MQ=255)

                                                                                                                 gTTGGCGTCGATGTCAAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                      <  2:4254919/150‑1 (MQ=255)

                                                                                                                   tGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgt                                    <  1:336867/150‑1 (MQ=255)

                                                                                                                     gCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATTGTCACGGCGCTCTGGATgtcg                                  >  1:3264861/1‑150 (MQ=255)

                                                                                                                       gTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcgtc                                >  2:2967435/1‑150 (MQ=255)

                                                                                                                       gTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcgtc                                <  2:6701234/150‑1 (MQ=255)

                                                                                                                       gTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcgtc                                <  1:891585/150‑1 (MQ=255)

                                                                                                                        tCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGtcgtcg                               >  1:7140121/1‑150 (MQ=255)

                                                                                                                           aTGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCa                            <  2:4345860/150‑1 (MQ=255)

                                                                                                                             gTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTg                                         <  1:2830043/135‑1 (MQ=255)

                                                                                                                                gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                       >  1:6193974/1‑150 (MQ=255)

                                                                                                                                gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                       <  2:1402539/150‑1 (MQ=255)

                                                                                                                                      cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                 <  1:108910/150‑1 (MQ=255)

                                                                                                                                      cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                 <  2:6923522/150‑1 (MQ=255)

                                                                                                                                       aCTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACAcc                >  2:2713742/1‑150 (MQ=255)

                                                                                                                                         ttCGATTTGCGGCACGCCGCGTGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg              >  1:3640013/1‑150 (MQ=255)

                                                                                                                                         ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg              >  1:4598138/1‑150 (MQ=255)

                                                                                                                                         ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg              >  1:2439192/1‑150 (MQ=255)

                                                                                                                                         ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg              >  1:3808632/1‑150 (MQ=255)

                                                                                                                                          tCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGc             <  2:3148636/150‑1 (MQ=255)

                                                                                                                                          tCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGc             <  1:2744689/150‑1 (MQ=255)

                                                                                                                                             atatGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCGGCACGTGAATGGTCACGGCGCTCTGGTTGTCGGCGGCAGTCGAGAACACCTGCGAc          >  2:7411733/4‑150 (MQ=255)

                                                                                                                                             aTTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGAc          <  1:7891096/150‑1 (MQ=255)

                                                                                                                                                tGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtc       <  2:523207/150‑1 (MQ=255)

                                                                                                                                                 gCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtct      >  1:1015648/1‑150 (MQ=255)

                                                                                                                                                   ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg    <  1:4959907/150‑1 (MQ=255)

                                                                                                                                                   ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg    <  1:6835438/150‑1 (MQ=255)

GCGTCACGCACCATACGCTCGATCTCTTCGTCCGACAGGCCGGAGTTGGCCTTGATCACGATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGT  >  NC_002947/5377979‑5378277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`