BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,188	C→G	9.2%	A449A (GCG→GCC)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,188	0	C	G	9.2%	1170.3 / 81.4	360	A449A (GCG→GCC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base C (138/189); new base G (19/14); total (157/203)
Fisher's exact test for biased strand distribution p-value = 9.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 368 total aligned reads displayed.

ATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACC  >  NC_002947/5378039‑5378337

    aCTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAg                                                                                                                                                   >  1:3443345/1‑150 (MQ=255)

      tGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGcc                                                                                                                                                 >  1:7053004/1‑150 (MQ=255)

      tGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGcc                                                                                                                                                 >  1:5995762/1‑150 (MQ=255)

       gttccTTGCCTGTGCCCTTGTCCTTTGCGGACACGTTCACGATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGcca                                                                                                                                                >  1:8979727/5‑149 (MQ=255)

          tCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCt                                                                                                                                             <  1:1162540/150‑1 (MQ=255)

            ttGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTa                                                                                                                                           >  1:7394317/1‑150 (MQ=255)

                cGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCt                                                                                                                                       <  2:9060000/150‑1 (MQ=255)

                 ggTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTc                                                                                                                                      >  2:1825281/1‑150 (MQ=255)

                 ggTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTc                                                                                                                                      >  1:6535966/1‑150 (MQ=255)

                 ggTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTCCTGCGCAGCCTGCTTACGCTc                                                                                                                                      <  2:499761/150‑1 (MQ=255)

                       cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                                                                                >  1:2430498/1‑150 (MQ=255)

                       cTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTg                                                                                                                                <  1:8705161/150‑1 (MQ=255)

                        ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                                                                               <  1:7566204/150‑1 (MQ=255)

                        ttGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCAAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgc                                                                                                                               >  1:319136/1‑150 (MQ=255)

                         tGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgca                                                                                                                              <  1:7259181/150‑1 (MQ=255)

                         tGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgca                                                                                                                              <  2:7359973/150‑1 (MQ=255)

                           tCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTgcagc                                                                                                                            >  2:8078717/1‑150 (MQ=255)

                                  cACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGaa                                                                                                                     <  1:4645361/150‑1 (MQ=255)

                                   aCCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAAt                                                                                                                    <  2:3929901/150‑1 (MQ=255)

                                      tACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGt                                                                                                                 >  1:2340903/1‑150 (MQ=255)

                                             acgATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgcc                                                                                                          <  1:1518794/148‑2 (MQ=255)

                                               gATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGct                                                                                                        >  2:2744689/1‑150 (MQ=255)

                                               gATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGct                                                                                                        >  1:6201603/1‑150 (MQ=255)

                                               gATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccga                                                                                                        <  2:2361449/150‑4 (MQ=255)

                                                  gCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTg                                                                                                     >  2:7933233/1‑150 (MQ=255)

                                                   ccGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTgg                                                                                                    >  2:6662308/1‑150 (MQ=255)

                                                   ccGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTgg                                                                                                    >  1:254479/1‑150 (MQ=255)

                                                   ccGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccgactga                                                                                                    <  2:3031710/150‑8 (MQ=255)

                                                     gTTGGCGTCGATGTCGAAGGTCACTTCGATCTGCGGCACGCCACGCGGTGCGGGCGGAATGCCGACGAGGTCGAACTGGCCGAGCATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAAGACGCGGATCGTCACGGCCGACTGGtt                                                                                                  <  2:8570898/150‑1 (MQ=255)

                                                     gTTGGCGTCGATGTCAAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                                                                                  <  2:4254919/150‑1 (MQ=255)

                                                       tGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgt                                                                                                <  1:336867/150‑1 (MQ=255)

                                                         gCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATTGTCACGGCGCTCTGGATgtcg                                                                                              >  1:3264861/1‑150 (MQ=255)

                                                           gTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcgtc                                                                                            <  2:2887682/150‑1 (MQ=255)

                                                               aTGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCa                                                                                        <  2:4345860/150‑1 (MQ=255)

                                                                          cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                                                                             <  2:6923408/150‑1 (MQ=255)

                                                                            cTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCt                                                                           >  2:4032261/1‑150 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGTGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          >  1:3640013/1‑150 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          >  2:4113128/1‑150 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          >  1:5045252/1‑150 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          >  1:4598076/1‑150 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          >  1:3808632/1‑150 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          >  1:7941098/1‑150 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          <  1:8086558/150‑1 (MQ=255)

                                                                             ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTACAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                          >  2:2242867/1‑150 (MQ=255)

                                                                              tCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGc                                                                         <  2:3148636/150‑1 (MQ=255)

                                                                                     gCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtct                                                                  >  1:1015648/1‑150 (MQ=255)

                                                                                       ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                                                <  1:6835438/150‑1 (MQ=255)

                                                                                       ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                                                >  1:7847519/1‑150 (MQ=255)

                                                                                       ggCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTg                                                                <  1:2211854/150‑1 (MQ=255)

                                                                                        gCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTTCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTgg                                                               >  2:7145815/1‑150 (MQ=255)

                                                                                        gCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTgg                                                               >  1:2785330/1‑150 (MQ=255)

                                                                                          aCGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTc                                                             >  1:399896/1‑150 (MQ=255)

                                                                                            gccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCgg                                                           <  1:2946509/150‑1 (MQ=255)

                                                                                             ccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCggg                                                          <  1:984795/150‑1 (MQ=255)

                                                                                              cgcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGa                                                         <  2:1119050/150‑1 (MQ=255)

                                                                                              cgcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGa                                                         <  1:5272409/150‑1 (MQ=255)

                                                                                              cgcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGa                                                         <  2:3205905/150‑1 (MQ=255)

                                                                                                cgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtg                                                       <  2:8279969/150‑1 (MQ=255)

                                                                                                  cgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggt                                                     <  2:6768281/150‑1 (MQ=255)

                                                                                                  cgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggt                                                     <  2:6355312/150‑1 (MQ=255)

                                                                                                  cgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTGGGTCGGGAtggt                                                     <  2:8843841/150‑1 (MQ=255)

                                                                                                   ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                    <  1:8694748/150‑1 (MQ=255)

                                                                                                   ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                    <  2:7067597/150‑1 (MQ=255)

                                                                                                   ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                    <  2:4868415/150‑1 (MQ=255)

                                                                                                      gCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGgtggtg                                                 <  1:5812918/150‑1 (MQ=255)

                                                                                                         ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                                              <  2:3499574/150‑1 (MQ=255)

                                                                                                         ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                                              <  2:8710883/150‑1 (MQ=255)

                                                                                                            ggAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttcttc                                           <  2:7650110/150‑1 (MQ=255)

                                                                                                             gAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                                          <  1:4422914/150‑1 (MQ=255)

                                                                                                                 gTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGAt                                      >  2:5307776/1‑150 (MQ=255)

                                                                                                                 gTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGAt                                      <  1:6129197/150‑1 (MQ=255)

                                                                                                                  tCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATc                                     >  1:5537150/1‑150 (MQ=255)

                                                                                                                     gCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgc                                  >  1:8660508/1‑150 (MQ=255)

                                                                                                                      ccAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgcg                                 >  2:6744663/1‑150 (MQ=255)

                                                                                                                      ccAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGATCTTCTCGATCAgcg                                 >  2:1496352/1‑150 (MQ=255)

                                                                                                                       cAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTTCTTACGCTCACCTTGCATCACGTGAATTGTCACGGCGCTCTGGTTGTCGTGGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTATCGATCAgcgc                                <  1:83208/150‑1 (MQ=255)

                                                                                                                       cAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgcgc                                <  1:1825281/150‑1 (MQ=255)

                                                                                                                          gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                             >  2:3680178/1‑150 (MQ=255)

                                                                                                                           tCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtc                            <  2:4162965/150‑1 (MQ=255)

                                                                                                                                cTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAc                       <  1:5149213/150‑1 (MQ=255)

                                                                                                                                 ttGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcg                      >  2:5067662/1‑150 (MQ=255)

                                                                                                                                 ttGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcg                      >  2:1220706/1‑150 (MQ=255)

                                                                                                                                   gCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                   >  2:7793185/1‑87 (MQ=255)

                                                                                                                                     ccAGCGACTTGATCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCAcgccgc                  >  2:3285177/1‑150 (MQ=255)

                                                                                                                                         cGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCAt              <  1:4167747/150‑1 (MQ=255)

                                                                                                                                         cGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCAt              <  2:3784/150‑1 (MQ=255)

                                                                                                                                          gACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATg             <  1:4920696/150‑1 (MQ=255)

                                                                                                                                             ttGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTGGTCGTCGGCAGGCGAGAACACCTGCGCCGTCTTGGGCGGGAGGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGtt          >  2:4761973/1‑150 (MQ=255)

                                                                                                                                              tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt         <  1:4212315/150‑1 (MQ=255)

                                                                                                                                              tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt         <  1:7743150/150‑1 (MQ=255)

                                                                                                                                              tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt         <  1:6306296/150‑1 (MQ=255)

                                                                                                                                              tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt         <  2:8946374/150‑1 (MQ=255)

                                                                                                                                               gTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTc        <  2:6218822/150‑1 (MQ=255)

                                                                                                                                                 tCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGa      <  1:2108455/150‑1 (MQ=255)

                                                                                                                                                  cTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGAt     <  1:9070121/150‑1 (MQ=255)

                                                                                                                                                   tGCGCAGCCTGCTTACGCTCACCTTTCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa    >  1:1631122/1‑150 (MQ=255)

                                                                                                                                                   tGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa    <  2:254479/150‑1 (MQ=255)

                                                                                                                                                   tGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa    >  2:3846985/1‑150 (MQ=255)

                                                                                                                                                     cgcAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGa                                                                       >  2:5894059/1‑81 (MQ=255)

                                                                                                                                                     cgcAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATAcc  >  1:348138/1‑150 (MQ=255)

ATCGACTGAGTCTTGCCGGTGGCCTTGTCTTTCGCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACC  >  NC_002947/5378039‑5378337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`