BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,221	A→G	6.8%	I438I (ATT→ATC)	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,221	0	A	G	6.8%	1110.7 / 43.7	352	I438I (ATT→ATC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base A (132/196); new base G (11/13); total (143/209)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 360 total aligned reads displayed.

GCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGA  >  NC_002947/5378072‑5378369

gCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGa                                                                                                                                                      >  1:1767029/1‑150 (MQ=255)

 cACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGaa                                                                                                                                                     <  1:4644979/150‑1 (MQ=255)

 cACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGaa                                                                                                                                                     <  1:3780376/150‑1 (MQ=255)

  aCCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTAGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAAt                                                                                                                                                    <  1:8090793/150‑1 (MQ=255)

   ccTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATg                                                                                                                                                   <  1:8670658/150‑1 (MQ=255)

     tACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGt                                                                                                                                                 >  2:1154260/1‑150 (MQ=255)

         ttcacgATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACg                                                                                                                                             >  1:6972673/6‑150 (MQ=255)

         tGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGTACGTGAATGGTCACg                                                                                                                                             <  1:3444146/150‑1 (MQ=255)

          gCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACgg                                                                                                                                            <  2:4240265/150‑1 (MQ=255)

              gATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccga                                                                                                                                        >  1:5110960/1‑147 (MQ=255)

              gATACCGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccga                                                                                                                                        <  2:2361449/150‑4 (MQ=255)

                 gCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTg                                                                                                                                     <  1:5955771/150‑1 (MQ=255)

                 gCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTg                                                                                                                                     <  1:4070685/150‑1 (MQ=255)

                  ccGTTGGCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGgccgactga                                                                                                                                    <  2:3031710/150‑8 (MQ=255)

                    gTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGtt                                                                                                                                  <  2:5968781/150‑1 (MQ=255)

                    gTTGGCGTCGATGTCGAAGGTCACTTCGATCTGCGGCACGCCACGCGGTGCGGGCGGAATGCCGACGAGGTCGAACTGGCCGAGCATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAAGACGCGGATCGTCACGGCCGACTGGtt                                                                                                                                  <  2:8570898/150‑1 (MQ=255)

                     ttGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTg                                                                                                                                 <  2:3940449/150‑1 (MQ=255)

                     ttGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTg                                                                                                                                 <  2:5698412/150‑1 (MQ=255)

                      tGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgt                                                                                                                                <  1:336832/150‑1 (MQ=255)

                       ggCGTCGATGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGGGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTgtc                                                                                                                               <  2:9039800/150‑1 (MQ=255)

                        gCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATTGTCACGGCGCTCTGGATgtcg                                                                                                                              >  1:3264861/1‑150 (MQ=255)

                          gTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcgtc                                                                                                                            <  1:1294043/150‑1 (MQ=255)

                          gTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTgtcgtc                                                                                                                            >  2:2967435/1‑150 (MQ=255)

                              aTGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCa                                                                                                                        <  2:7918251/150‑1 (MQ=255)

                              aTGTCGAAGGTCACTTCGATCTGTGGAACGCCACGCGGTGCCGGCGGAATGCCAACGAGGTCGAACTGGCCGAGGATCTTGTTGTCGGCAGCCATTTCACGCTCGCCCTGGAACACGCGGATCGTCACGGCCGACTGATTGTCTTCAGcg                                                                                                                        >  1:6823426/1‑149 (MQ=255)

                                 tCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTc                                                                                                                     <  1:4544707/150‑1 (MQ=255)

                                   gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                                                   <  1:6274656/150‑1 (MQ=255)

                                   gAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCga                                                                                                                   >  1:6193974/1‑150 (MQ=255)

                                    aaGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCgag                                                                                                                  <  2:4538880/150‑1 (MQ=255)

                                         cACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAacac                                                                                                             <  1:8190523/150‑1 (MQ=255)

                                          aCTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACAcc                                                                                                            >  1:1884304/1‑150 (MQ=255)

                                           cTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCt                                                                                                           >  2:4032261/1‑150 (MQ=255)

                                            ttCGATTTGCGGCACGCCGCGTGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                          >  1:3640013/1‑150 (MQ=255)

                                            ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                          >  2:5004835/1‑150 (MQ=255)

                                            ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                          >  2:7743150/1‑150 (MQ=255)

                                            ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                          <  2:3216866/150‑1 (MQ=255)

                                            ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                          >  2:2721816/1‑150 (MQ=255)

                                            ttCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTACAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTg                                                                                                          >  2:2242867/1‑150 (MQ=255)

                                             tCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGc                                                                                                         <  2:6623301/150‑1 (MQ=255)

                                             tCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGc                                                                                                         <  2:3148636/150‑1 (MQ=255)

                                                    gCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGActtct                                                                                                  >  1:1015648/1‑150 (MQ=255)

                                                          cgccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCg                                                                                            <  2:879727/150‑1 (MQ=255)

                                                          cgccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCg                                                                                            <  2:4832786/150‑1 (MQ=255)

                                                           gccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCgg                                                                                           <  1:2946509/150‑1 (MQ=255)

                                                            ccgcGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCggg                                                                                          >  2:7514987/1‑150 (MQ=255)

                                                             cgcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGa                                                                                         <  2:8005347/150‑1 (MQ=255)

                                                             cgcgcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGa                                                                                         <  2:7189204/150‑1 (MQ=255)

                                                                gcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtgg                                                                                      <  1:1591035/150‑1 (MQ=255)

                                                                gcgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtgg                                                                                      <  2:3739937/150‑1 (MQ=255)

                                                                 cgGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggt                                                                                     <  2:6768281/150‑1 (MQ=255)

                                                                  ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                                                    <  2:4878432/150‑1 (MQ=255)

                                                                  ggTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGAtggtg                                                                                    <  1:5256026/150‑1 (MQ=255)

                                                                        ggCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGttc                                                                              >  1:7433359/1‑150 (MQ=255)

                                                                            gAATGTCAGCCAGGTGGTACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGGGTtcttct                                                                          <  2:4112190/150‑1 (MQ=255)

                                                                            gAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTtcttct                                                                          <  1:4422914/150‑1 (MQ=255)

                                                                             aaTGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTtctc                                                                         <  1:7815221/150‑1 (MQ=255)

                                                                                 tCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATc                                                                     >  1:5537150/1‑150 (MQ=255)

                                                                                    gCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgc                                                                  <  2:414671/150‑1 (MQ=255)

                                                                                     ccAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAgcg                                                                 >  2:2099567/1‑150 (MQ=255)

                                                                                        ggTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCgg                                                              >  2:3336964/1‑150 (MQ=255)

                                                                                         gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                                                             >  1:4189784/1‑150 (MQ=255)

                                                                                         gTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGt                                                             >  2:6714563/1‑150 (MQ=255)

                                                                                           cGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtca                                                           >  2:6920164/1‑150 (MQ=255)

                                                                                            gAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcat                                                          >  1:962531/1‑150 (MQ=255)

                                                                                             aaCTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcatc                                                         >  2:6311215/1‑150 (MQ=255)

                                                                                              aCTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcatca                                                        <  1:4313655/150‑1 (MQ=255)

                                                                                              aCTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcatca                                                        >  1:8795848/1‑150 (MQ=255)

                                                                                                     cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                                                 >  1:2455317/1‑150 (MQ=255)

                                                                                                      aGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcct                                                >  2:3544617/1‑149 (MQ=255)

                                                                                                        cGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCAt                                              <  2:3784/150‑1 (MQ=255)

                                                                                                         gACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATg                                             <  1:4920696/150‑1 (MQ=255)

                                                                                                             tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt                                         <  1:7743150/150‑1 (MQ=255)

                                                                                                                tCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGa                                      <  1:2108455/150‑1 (MQ=255)

                                                                                                                tCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGa                                      <  1:2205409/150‑1 (MQ=255)

                                                                                                                 cTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGAt                                     <  1:9070120/150‑1 (MQ=255)

                                                                                                                 cTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGAt                                     >  2:1254655/1‑150 (MQ=255)

                                                                                                                  tGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa                                    >  2:3847102/1‑150 (MQ=255)

                                                                                                                       aGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAg                               <  2:4335978/150‑1 (MQ=255)

                                                                                                                       aGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAg                               <  1:8460319/150‑1 (MQ=255)

                                                                                                                        gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                              >  2:8866229/1‑150 (MQ=255)

                                                                                                                         ccTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAggg                             >  2:697915/1‑150 (MQ=255)

                                                                                                                              ttACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGc                        >  1:5672315/1‑150 (MQ=255)

                                                                                                                                 cGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCggg                     <  2:2430498/150‑1 (MQ=255)

                                                                                                                                  gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                    <  2:2537911/150‑1 (MQ=255)

                                                                                                                                  gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                    >  1:7937567/1‑150 (MQ=255)

                                                                                                                                  gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                    >  2:8678007/1‑150 (MQ=255)

                                                                                                                                  gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                    >  1:2795900/1‑150 (MQ=255)

                                                                                                                                   cTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCt                   >  2:8226155/1‑150 (MQ=255)

                                                                                                                                    tCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg                  >  1:3542380/1‑150 (MQ=255)

                                                                                                                                    tCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg                  >  1:2367567/1‑150 (MQ=255)

                                                                                                                                      aCCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGAc                <  2:1882029/150‑1 (MQ=255)

                                                                                                                                        cTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGt              >  1:6422213/1‑150 (MQ=255)

                                                                                                                                         ttGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTc             >  2:274639/1‑150 (MQ=255)

                                                                                                                                         ttGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTc             >  2:1415669/1‑150 (MQ=255)

                                                                                                                                          tGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTcc            <  1:6152024/150‑1 (MQ=255)

                                                                                                                                          tGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCCCGCTGAccctg            <  2:5131239/150‑5 (MQ=255)

                                                                                                                                           gcagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCCCGCTGAccctgg           <  2:6507178/150‑6 (MQ=255)

                                                                                                                                            cagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcag          <  1:198416/150‑1 (MQ=255)

                                                                                                                                                  gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcagcagcag    <  2:7029693/150‑1 (MQ=255)

                                                                                                                                                   tGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAgg   <  2:1062118/150‑1 (MQ=255)

GCACCTACGTGCAGGATGCCGTTGGCGTCGATGTCGAAGGTCACTTCGATTTGCGGCACGCCGCGCGGTGCTGGCGGAATGTCAGCCAGGTCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGA  >  NC_002947/5378072‑5378369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`