BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,378,311	C→G	5.0%	M408I (ATG→ATC) ‡	dnaK ←	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,378,311	0	C	G	5.0%	877.8 / 25.6	258	M408I (ATG→ATC) ‡	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base C (103/142); new base G (3/10); total (106/152)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 276 total aligned reads displayed.

TCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTGCGCGCTTCTTT  >  NC_002947/5378162‑5378460

   aaCTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTCCGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcatc                                                                                                                                                    <  2:3448910/150‑1 (MQ=255)

    aCTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGtcatca                                                                                                                                                   >  1:8795848/1‑150 (MQ=255)

           cAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACgccgcc                                                                                                                                            >  1:3396691/1‑150 (MQ=255)

              cGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCAt                                                                                                                                         <  2:3784/150‑1 (MQ=255)

              cGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCAt                                                                                                                                         <  1:4167747/150‑1 (MQ=255)

               gACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATg                                                                                                                                        <  1:4920696/150‑1 (MQ=255)

                   tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt                                                                                                                                    <  2:8946362/150‑1 (MQ=255)

                   tGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGttt                                                                                                                                    <  1:2083493/150‑1 (MQ=255)

                    gTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTc                                                                                                                                   <  2:6218822/150‑1 (MQ=255)

                    gTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTc                                                                                                                                   <  2:319136/150‑1 (MQ=255)

                      tCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGa                                                                                                                                 <  1:2205409/150‑1 (MQ=255)

                       cTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGAt                                                                                                                                >  2:1254655/1‑150 (MQ=255)

                       cTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGAt                                                                                                                                <  1:9070121/150‑1 (MQ=255)

                        tGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa                                                                                                                               >  2:3847102/1‑150 (MQ=255)

                        tGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa                                                                                                                               <  2:254479/150‑1 (MQ=255)

                              gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                                                                                         <  2:3586506/150‑1 (MQ=255)

                              gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                                                                                         <  2:2381398/150‑1 (MQ=255)

                              gCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAgg                                                                                                                         <  2:2335131/150‑1 (MQ=255)

                                    ttACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGc                                                                                                                   <  1:1782984/150‑1 (MQ=255)

                                    ttACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGc                                                                                                                   >  1:5672315/1‑150 (MQ=255)

                                     tACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCg                                                                                                                  <  1:5983002/150‑1 (MQ=255)

                                       cGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCggg                                                                                                                <  2:4537994/150‑1 (MQ=255)

                                       cGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCggg                                                                                                                <  2:2202393/150‑1 (MQ=255)

                                        gCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGc                                                                                                               >  2:8678007/1‑150 (MQ=255)

                                         cTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCt                                                                                                              <  2:4142661/150‑1 (MQ=255)

                                          tCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg                                                                                                             <  1:3064695/150‑1 (MQ=255)

                                          tCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg                                                                                                             >  2:6932651/1‑150 (MQ=255)

                                            aCCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGAc                                                                                                           <  2:1882029/150‑1 (MQ=255)

                                            aCCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGAc                                                                                                           >  1:805546/1‑150 (MQ=255)

                                              cTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGt                                                                                                         >  1:6422213/1‑150 (MQ=255)

                                              cTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGt                                                                                                         >  2:8432592/1‑150 (MQ=255)

                                               gtGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCCCGCTGAccct                                                                                                        <  2:4965231/149‑4 (MQ=255)

                                                tGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTcc                                                                                                       <  1:6152599/150‑1 (MQ=255)

                                                 gcagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCCCGCTGAccctgg                                                                                                      <  2:7797313/150‑6 (MQ=255)

                                                 gcagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCCCGCTGAccctgg                                                                                                      <  2:6507178/150‑6 (MQ=255)

                                                  cagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcag                                                                                                     <  1:198416/150‑1 (MQ=255)

                                                  cagcaCGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCcag                                                                                                     <  2:2455317/150‑1 (MQ=255)

                                                    acaCGCGGATCGTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCgagca                                                                                                   <  1:8418817/149‑1 (MQ=255)

                                                          gAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGa                                                                                             >  1:4195329/1‑150 (MQ=255)

                                                          gAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGa                                                                                             <  1:4320239/150‑1 (MQ=255)

                                                           actGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGAc                                                                                            <  2:8128340/148‑1 (MQ=255)

                                                             tcgTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGt                                                                                          >  1:4374321/3‑150 (MQ=255)

                                                              cgTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCTGAACGTc                                                                                         <  2:2131293/149‑1 (MQ=255)

                                                              cgTCACGGCCGACTGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTc                                                                                         <  2:5110960/149‑1 (MQ=255)

                                                               gTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCt                                                                                        <  2:2814434/150‑1 (MQ=255)

                                                                tCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACg                                                                                                          <  1:6804180/131‑1 (MQ=255)

                                                                tCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACg                                                                                                          >  2:6804180/1‑131 (MQ=255)

                                                                  aCGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTc                                                                                     <  2:5995762/150‑1 (MQ=255)

                                                                  aCGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTc                                                                                     <  2:1392502/150‑1 (MQ=255)

                                                                         actGATTGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTcgc                                                                              >  2:1701187/2‑148 (MQ=255)

                                                                           tGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCg                                                                            >  1:7175716/1‑150 (MQ=255)

                                                                            gattGTCTTCAGCGGTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTCGccct                                                                           <  1:4822430/148‑3 (MQ=255)

                                                                             gTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGc                                                                          <  1:7933233/150‑1 (MQ=255)

                                                                              ttGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGcc                                                                         >  1:451036/1‑150 (MQ=255)

                                                                                   gtcgGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTAc                                                                    >  1:2491518/1‑150 (MQ=255)

                                                                                   gtcgGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTAc                                                                    >  1:2491638/1‑150 (MQ=255)

                                                                                           tCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCt                                                            >  1:251229/1‑150 (MQ=255)

                                                                                              agaACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGa                                                         <  2:6193974/150‑1 (MQ=255)

                                                                                              agaACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGa                                                         <  2:7393602/150‑1 (MQ=255)

                                                                                                  cacCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGgc                                                     <  2:3640013/150‑1 (MQ=255)

                                                                                                   acCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGgca                                                    <  2:3325803/150‑1 (MQ=255)

                                                                                                   acCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGgca                                                    <  2:7941098/150‑1 (MQ=255)

                                                                                                    ccTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGgcag                                                   >  1:1626029/1‑150 (MQ=255)

                                                                                                     cTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCg                                                                            <  2:7497923/124‑1 (MQ=255)

                                                                                                     cTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCg                                                                            >  1:7497923/1‑124 (MQ=255)

                                                                                                     cTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGgcagc                                                  <  1:2403641/150‑1 (MQ=255)

                                                                                                           cttcttGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCAt                                            <  1:3777267/150‑1 (MQ=255)

                                                                                                           cttcttGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCAt                                            <  1:5004985/150‑1 (MQ=255)

                                                                                                             tcttGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATgg                                          <  2:1899587/150‑1 (MQ=255)

                                                                                                               ttGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCa                                        >  2:6992372/1‑150 (MQ=255)

                                                                                                               ttGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCa                                        >  2:6707739/1‑150 (MQ=255)

                                                                                                               ttGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCa                                        >  2:1546891/1‑150 (MQ=255)

                                                                                                                tGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCaa                                       <  1:8226155/150‑1 (MQ=255)

                                                                                                                  gTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACg                                     >  2:8170995/1‑150 (MQ=255)

                                                                                                                   tCGGGATGGTGGTGTTCTTCTCGTTCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACgg                                    >  2:463999/1‑150 (MQ=255)

                                                                                                                       gATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTc                                <  1:6920164/150‑1 (MQ=255)

                                                                                                                       gATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTc                                <  2:4076678/150‑1 (MQ=255)

                                                                                                                       gATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTc                                <  2:7140121/150‑1 (MQ=255)

                                                                                                                       gATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTc                                <  1:1154260/150‑1 (MQ=255)

                                                                                                                            tggtgTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCg                           <  1:2967435/150‑1 (MQ=255)

                                                                                                                            tggtgTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCg                           >  1:3493388/1‑150 (MQ=255)

                                                                                                                            tggtgTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCg                           <  1:4032261/150‑1 (MQ=255)

                                                                                                                             ggtgTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCgg                          >  1:5097797/1‑150 (MQ=255)

                                                                                                                             ggtgTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCgg                          >  1:8883488/1‑150 (MQ=255)

                                                                                                                              gtgtTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCggg                         >  2:7160284/1‑150 (MQ=255)

                                                                                                                                gtTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGtt                       >  2:1120356/1‑150 (MQ=255)

                                                                                                                                   cttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGAc                    <  1:1496352/150‑1 (MQ=255)

                                                                                                                                   cttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGAc                    <  1:2721816/150‑1 (MQ=255)

                                                                                                                                   cttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGAc                    <  1:6662308/150‑1 (MQ=255)

                                                                                                                                   cttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGAc                    <  1:7341451/150‑1 (MQ=255)

                                                                                                                                   cttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGAc                    <  1:7758999/150‑1 (MQ=255)

                                                                                                                                    ttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACg                   >  1:540255/1‑150 (MQ=255)

                                                                                                                                    ttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACg                   >  2:4775205/1‑150 (MQ=255)

                                                                                                                                    ttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACg                   >  2:5677376/1‑150 (MQ=255)

                                                                                                                                         gATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCtt              >  1:5208400/1‑150 (MQ=255)

                                                                                                                                         gATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCtt              >  2:421073/1‑150 (MQ=255)

                                                                                                                                         gATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCtt              >  2:5205516/1‑150 (MQ=255)

                                                                                                                                             aGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTgcgc          >  1:6279369/1‑150 (MQ=255)

                                                                                                                                              gcgcGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTgcgcg         >  2:6335155/1‑150 (MQ=255)

                                                                                                                                               cgcgGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTgcgcgc        <  2:1015648/150‑1 (MQ=255)

TCGAACTTGCCCAGCGACTTGTTCTGCGCAGCCTGCTTACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTGCGCGCTTCTTT  >  NC_002947/5378162‑5378460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`