Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,448,268 A→C 12.2% Q148P (CAG→CCG)  thiE → thiamine‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,448,2680AC12.2% 562.3 / 8.3 264Q148P (CAG→CCG) thiEthiamine‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (150/72);  new base C (11/29);  total (161/103)
Fisher's exact test for biased strand distribution p-value = 2.66e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

CAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACCCGCCGCG  >  NC_002947/5448120‑5448417
                                                                                                                                                    |                                                                                                                                                     
cAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAg                                                                                                                                                      <  2:8715249/150‑1 (MQ=255)
 aGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCCACGTCGGCCGGCGCGCGCAgg                                                                                                                                                     >  1:6540613/1‑150 (MQ=255)
  ggCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGc                                                                                                                                                    <  2:6499842/150‑1 (MQ=255)
   gCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGcc                                                                                                                                                   >  2:3237045/1‑150 (MQ=255)
    cAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAGCGTCGGCCTGCTCGAGCAGGccg                                                                                                                                                  <  1:1004485/150‑2 (MQ=255)
     aaTCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCACGcccc                                                                                                                                                 >  1:7318884/1‑149 (MQ=255)
     aaTCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCGGCCAACGTCGCCCTGCGCGCGCCGGcccc                                                                                                                                                 >  2:6340188/1‑149 (MQ=255)
       tcatcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCATGCTGCCAGCGAAGGCGCCAGCTACGTGGCATTTGGCCGCTTCTTAAATTCTGTCACCAAGCCGGGGGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCTGGccgacg                                                                                                                                               <  2:2361789/150‑5 (MQ=255)
       tcatcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGCGCAGGCCcgcg                                                                                                                                               >  2:3055552/1‑150 (MQ=255)
       tcatcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCAGCTCGAGCAGGCCcgcg                                                                                                                                               >  2:5985446/1‑150 (MQ=255)
         atcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGcc                                                                                                                                             >  1:7864643/1‑150 (MQ=255)
          tcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGACTGCTCGAGCAGGCCCGCGccc                                                                                                                                            >  2:784868/1‑150 (MQ=255)
             gCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCgg                                                                                                                                         >  2:5953349/1‑150 (MQ=255)
               tCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGCGCCGGCCCGCGcccccggg                                                                                                                                       >  1:6873079/1‑145 (MQ=255)
               tCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGCGCCGGCCCCCCCCCacggg                                                                                                                                       >  1:6252077/1‑146 (MQ=255)
                  aCCTGCCACGCCAGCCTCGAACTGGCCGCCCCGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTATTCAATCCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGaaa                                                                                                                                    <  1:7489586/150‑1 (MQ=255)
                  aCCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTGGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGaaa                                                                                                                                    <  2:2040246/150‑1 (MQ=255)
                    cTGCCACGCCTGCCTCTACCGGGCGGCCCAGGCTGCCCGCGATGGGGCCAGCTACTTGGCCTTTGGCCGCTTCTTCAATTCGGTCCCCACGCCGGGCGCGCCCGCGGCCACGGCCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACt                                                                                                                                  <  2:5401289/150‑1 (MQ=255)
                    cTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAAAGTCGGGGTGCTCGAGCAGGCCCGCGCCCCCGTGAAACt                                                                                                                                  >  1:3110474/1‑150 (MQ=255)
                      gCCCCGCCTGCCTCGAACGGGCCGCCCCGGCTGCCTGCTATGGCGCCAGATACGGGGCGTTTGGCCGCTTCTTAAATTCTGTCACCAAGCCGGGCGCCCCCGCTGCAACCGTCGGCTGGCGCGCGCAGGCCCGCGCCCAGGTGAAACTGc                                                                                                                                <  2:3812922/150‑1 (MQ=255)
                       ccACGCCAGCCTCGAACTGGCCGCCCTGGCTGCCAGCGACGGGGCCAGCTACGTGGCCTTGGGCCGCTTCTTCAATTCTGTCACCAACCCGGGCGCGCCCGCGGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGcc                                                                                                                               <  2:7705322/150‑1 (MQ=255)
                             cAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGc                                                                                                                         >  2:7314837/1‑150 (MQ=255)
                              aGCCTCGAACGGGCCGCCCCGGCTGCCAGCGATGGCGCCAGCTACGGGGCCTTTGGCCGCTTCTTCAATTCTGTCCCCACGCCGGGCGCGCCCGCGGCCACCGTGGGCGTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGcc                                                                                                                        <  2:7441801/150‑1 (MQ=255)
                              aGCCTCGAACGGGCCGCCCAGGCTGCCAGCGACGGCGCCAGCTACGGGGCCTTTGGCCGCTACTCCATTTCTGCCACCAAGCGGGGCGCGCCCGCGGCCACCGCCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGcc                                                                                                                        <  2:1771726/150‑1 (MQ=255)
                                 cTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCggg                                                                                                                     >  2:134004/1‑148 (MQ=255)
                                  tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGACACTGCCGATCGCCGTgg                                                                                                                    >  2:7161935/1‑149 (MQ=255)
                                  tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGa                                                                                                                    >  2:6704484/1‑150 (MQ=255)
                                     aaCTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGGGCCGGCCCCCCCCCCCCGGAAACTGCCGATAGCCGTGATCg                                                                                                                 >  1:3022936/1‑150 (MQ=255)
                                        gggCCGCCCAGGCTGCCACCGACGGCGCCAGCTACGGGGCCTTGGGCCGCTTCTTCAATTCTGCCACCAAGCCGGGCGCGCCCGCGGCCACCGCCGGCCGGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTg                                                                                                              <  1:2826803/149‑1 (MQ=255)
                                         ggCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCGTGATCGGTgg                                                                                                             >  2:5594789/1‑150 (MQ=255)
                                          gccgccCTGGCTGCCTGCGACGGCGCCCGCTCCGTGGCGTTTGGCCGCTACTACAAGCCGGACCCCACGCGGGGCGCGCCCGCGGCCACCGACGGCCGGCTCCAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGc                                                                                                            <  1:5730547/150‑1 (MQ=255)
                                            cgccCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGCGCAGGCCCGCGCCCACGTGCAACAGCCGATCGCCGTGGTCGGTGGCAt                                                                                                          >  1:7030950/1‑150 (MQ=255)
                                                 aGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAAATGCCGATCGCCGTGATCGGTTGCATCAccc                                                                                                     >  1:6953026/1‑150 (MQ=255)
                                                   gCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGGGTGCCGTGGCATCAcccgc                                                                                                   >  2:1936292/1‑148 (MQ=255)
                                                     tGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAACTGCCGCTCGCCGTGATCGGTGGCATCACCCACGa                                                                                                 >  2:2613940/1‑150 (MQ=255)
                                                        cAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGGGAAACTGCCGATCGCCGGGATCGGGGGCAACAcccaccacca                                                                                              >  1:5221782/1‑143 (MQ=255)
                                                          gCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGGCGGCGCGGGCAGGCCCGCGCCCACGTGAAACTGCCGCTCGCCGTGATCGGTGGCGGCACCCACCACAAcc                                                                                            >  1:8427069/1‑149 (MQ=255)
                                                          gCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCCCCCAGGTGAAAATGACGATCGCCGAGATCGGTGGCATCACCCAAGACAAcc                                                                                            >  1:1134291/1‑149 (MQ=255)
                                                            gAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCCGGCCCCCGCCCCGGGGAAACTGCCGATCGCCGGGATCGGTGGCGTCACCCACGACAAcgcc                                                                                          >  1:4248491/1‑150 (MQ=255)
                                                            gAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGGGTAACAGCCGATCGCCGGGATCGGTGGCATCACCCACGACAAagcc                                                                                          >  1:8936315/1‑150 (MQ=255)
                                                                gcgcCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCAGCACCCACGACAACGCCGccc                                                                                      >  1:1419357/1‑150 (MQ=255)
                                                                  gcCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCCCCCGCGACCACGCCGCCCCa                                                                                    >  2:869446/1‑150 (MQ=255)
                                                                    cAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGGCTGCTCGAGCAGGCCCGCGCCCACGTGAAACTGCCGATCGCCGTGATAGGTGGCATCACCCACGACAACCCCGccccccg                                                                                  >  1:3442030/1‑147 (MQ=255)
                                                                        tACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGGCTGGTGGAGCAGGGCCGCGCCCAGGTGTAAATGCCGATCGCCGTGATCGGTGGGATAACCCTCGACAAAGCCGCCCCACTGGtg                                                                              >  1:4432256/1‑149 (MQ=255)
                                                                           gTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCACGTGAAACAGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACGCCCCCCCACAGGTCGcc                                                                           >  1:8332249/1‑150 (MQ=255)
                                                                           gTGGCCTTGGGCCGCTTCTTCATTTCCGCCACCCCCCCGGGCGCGCCCGCGCCCAACGCCGGCCGGCCCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGcc                                                                           <  1:133555/150‑1 (MQ=255)
                                                                                 tttGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCGCCCCCGTGAAACAGCCCATCGCCCGGATAGGTGGCATCACCCACCACAACGCCGCCCCACAGGTCGCCCAcccc                                                                     >  1:7190191/1‑147 (MQ=255)
                                                                                    ggCCGCTTCTTCAAGTCTGTCCCCACGCCGGGCGCGCCCGCTGCCACCGTCGGCGTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGcc                                                                  <  2:8614166/150‑1 (MQ=255)
                                                                                      ccGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGa                                                                >  2:4630803/1‑150 (MQ=255)
                                                                                       cGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGAc                                                               >  2:6548069/1‑150 (MQ=255)
                                                                                        gCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAAATGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACCCCGCCCCACAGGTCGCCCACGGCGCCGAcc                                                              >  1:8747585/1‑150 (MQ=255)
                                                                                             ttcCATTCTGTCCCCCCGCCGGGCGCGCCCGCTGCCCCCGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACctgctg                                                         <  1:8131044/150‑1 (MQ=255)
                                                                                             ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGCGCAGGCCCCCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCCCCCGCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACctgctg                                                         >  2:5513129/1‑150 (MQ=255)
                                                                                             ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACACCGCCGCCCCACTGGTCGCCCACGGCGCCGACctgctg                                                         >  2:6234839/1‑150 (MQ=255)
                                                                                             ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAAAAGCCGATAGCCGTGATCGGTGGCATCAACCTCGACAACGCCCCCCCCCAGGTCGCCCACCGCGCCGACcagctg                                                         >  1:958300/1‑150 (MQ=255)
                                                                                             ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCACCACAACCCCGCCCCACAGGTCGCCCACGGCGCCGACcggctg                                                         >  1:2145054/1‑150 (MQ=255)
                                                                                             ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCACGAGCAGGCCCGCGCCCAGCGGTCAATGCCGATCGCCGAGGGCCGTGGCAGCACCCACGAAAACCCCCCCCCACGGCTCGCCCACCGCGCCGAccgcctg                                                         >  1:935829/1‑145 (MQ=255)
                                                                                              tcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGAACGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGCCGCCGACCAGCTgg                                                        >  2:7402165/1‑150 (MQ=255)
                                                                                              tcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCCTGATCGGTGGCATCACCCACGACAAAGCCGCCCCACAGGTCGCCCCCGGCGCCGCCCAGCTgg                                                        >  1:8625629/1‑150 (MQ=255)
                                                                                              tcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGTCTCGAGCACGCCCGCGCCCAGGTGAAACTGACCATAGCCGTGATCGGTGGCATCACCCACGACAACGCCCCCCCACAGGTCGCCCACCGCGCCGACCAGCTgg                                                        >  1:6323458/1‑150 (MQ=255)
                                                                                                aaTTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCCTCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCg                                                      >  2:8478760/1‑150 (MQ=255)
                                                                                                 aTTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCACGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACCCCGCCCCACAGGTCGCCCACCGCGCCGACCAGCTGGCgg                                                     >  1:2262128/1‑150 (MQ=255)
                                                                                                    cgcccaccacGCCGGCCCCCCCCGCCCCCCACGTCGGCCTGCTCGCCCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGa                                                  <  1:328891/146‑1 (MQ=255)
                                                                                                    cTGACACCAAGCCGGGCGCGCCCCCTCCCAACGACGCCCTGCTCCAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGGTCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGa                                                  <  2:5641096/150‑1 (MQ=255)
                                                                                                         cccccgCCGGGCGCGCCCGCGGCCCCCGGCGGCCTGCGCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                             <  1:4649090/145‑1 (MQ=255)
                                                                                                         aCCACGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                             <  1:5975704/150‑1 (MQ=255)
                                                                                                         aCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACACCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                             >  2:4974883/1‑150 (MQ=255)
                                                                                                           cAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCAGGCCCCCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCAACACCCTCGACAACGCCGCCCCACTGGGCGCCCACGGCGCCGACCGGCTGGCGGTGATCCACgc                                           >  2:4872382/1‑149 (MQ=255)
                                                                                                            aaGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGCGCAGGCCCGCGCCCACGGGAAACTGCCGATAGCCCTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACGGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGc                                          >  1:3984203/1‑150 (MQ=255)
                                                                                                            aaGCCGGGCGCGCCCGCCGCCACCGGCGGCCTGCTCGAGCAGACCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGc                                          <  2:4362392/150‑1 (MQ=255)
                                                                                                              gcggggCGCGCCCGCGGCCCCGGGGGGCCGGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCtt                                        <  1:1933824/147‑1 (MQ=255)
                                                                                                              gCCGGTCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCtt                                        <  2:3033035/150‑1 (MQ=255)
                                                                                                              gCCGGGCGCGCCCGCTGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCtt                                        <  2:2739750/150‑1 (MQ=255)
                                                                                                                 gggCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGtt                                     >  2:1528428/1‑150 (MQ=255)
                                                                                                                     gcgcCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGt                                 <  1:2993427/150‑1 (MQ=255)
                                                                                                                       gcCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGc                               >  2:4099588/1‑150 (MQ=255)
                                                                                                                       gcCCGCCGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGc                               <  1:8217364/150‑1 (MQ=255)
                                                                                                                       gcCCCCCGCCACCGGCGGCCGGCCCCCCCAGCCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGc                               <  1:5589386/150‑1 (MQ=255)
                                                                                                                               ccAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTg                                       >  2:4818113/1‑134 (MQ=255)
                                                                                                                               ccAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTg                                       <  1:4818113/134‑1 (MQ=255)
                                                                                                                               ccAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcg                       >  2:3508259/1‑150 (MQ=255)
                                                                                                                                cAACGTCGGCCTGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                      <  1:2899955/150‑1 (MQ=255)
                                                                                                                                cAACGTCGGCCTGCCCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                      <  2:5742134/150‑1 (MQ=255)
                                                                                                                                cAACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgc                      <  2:1153266/150‑1 (MQ=255)
                                                                                                                                 aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg                     >  2:8073591/1‑150 (MQ=255)
                                                                                                                                 aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCCACAACGCCGCCCCACTGGTCGCCCACGGCGCCCACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg                     >  1:8589630/1‑150 (MQ=255)
                                                                                                                                       ggCCTGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa               <  2:7227534/150‑1 (MQ=255)
                                                                                                                                       ggCCTGCTCGAGCAGGCCCGCGCCCATGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa               <  1:7314837/150‑1 (MQ=255)
                                                                                                                                       ggCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa               <  2:3762967/150‑1 (MQ=255)
                                                                                                                                         ccTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGt             >  1:5238780/1‑150 (MQ=255)
                                                                                                                                          cTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTc            >  1:4511457/1‑150 (MQ=255)
                                                                                                                                          cTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTc            >  1:2523714/1‑150 (MQ=255)
                                                                                                                                            gCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAc          >  2:4742351/1‑150 (MQ=255)
                                                                                                                                             cTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAcc         <  2:6825378/150‑1 (MQ=255)
                                                                                                                                             cTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAcc         <  2:8948728/150‑1 (MQ=255)
                                                                                                                                              tCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAccc        >  2:1998491/1‑150 (MQ=255)
                                                                                                                                              tCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAccc        >  1:2428756/1‑150 (MQ=255)
                                                                                                                                               cGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACccg       >  1:2075771/1‑150 (MQ=255)
                                                                                                                                                gAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACccgc      >  1:1701202/1‑150 (MQ=255)
                                                                                                                                                    aGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACCCGccgcg  >  2:7389963/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACCCGCCGCG  >  NC_002947/5448120‑5448417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑