BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	433,847	C→G	5.3%	V452V (GTG→GTC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	433,847	0	C	G	5.3%	1219.7 / 40.4	358	V452V (GTG→GTC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base C (163/176); new base G (10/9); total (173/185)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 369 total aligned reads displayed.

CCGGTGGCCAGGCCAACGTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTC  >  NC_002947/433698‑433996

      gccaggccaACGTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCAcg                                                                                                                                                 >  1:3358335/1‑150 (MQ=255)

       ccaggccaACGTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCAcgc                                                                                                                                                <  2:6666257/150‑1 (MQ=255)

        caggccaACGTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCAcgcg                                                                                                                                               >  1:5601638/1‑150 (MQ=255)

          ggccaACGTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCt                                                                                                                                             <  2:291625/150‑1 (MQ=255)

             caACGTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTcgg                                                                                                                                          <  1:6189901/150‑1 (MQ=255)

                 gTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCggcggc                                                                                                                                      >  1:2254973/1‑150 (MQ=255)

                 gTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCggcggc                                                                                                                                      >  2:7932675/1‑150 (MQ=255)

                    gACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGc                                                                                                                                   >  1:916124/1‑150 (MQ=255)

                    gACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGc                                                                                                                                   >  2:8679696/1‑150 (MQ=255)

                     aCGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGcc                                                                                                                                  <  1:4607638/150‑1 (MQ=255)

                      cGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCt                                                                                                                                 <  1:8965565/150‑1 (MQ=255)

                       gttgttgttTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCCCGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCtt                                                                                                                                <  1:5267071/150‑1 (MQ=255)

                            tgttTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGc                                                                                                                           >  1:1148328/1‑150 (MQ=255)

                             gttTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCa                                                                                                                          <  1:8075132/150‑1 (MQ=255)

                              ttttCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAg                                                                                                                         >  1:1692225/1‑150 (MQ=255)

                                   gTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACtt                                                                                                                    <  1:7482499/150‑1 (MQ=255)

                                   gTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACtt                                                                                                                    <  2:4582229/150‑1 (MQ=255)

                                   gTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACtt                                                                                                                    <  2:6114268/150‑1 (MQ=255)

                                     aGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                  <  2:8401903/150‑1 (MQ=255)

                                     aGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                  <  2:878198/150‑1 (MQ=255)

                                     aGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGa                                                                                                                  <  2:1030877/150‑1 (MQ=255)

                                              tCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCg                                                                                                         >  1:8896829/1‑150 (MQ=255)

                                                 aCTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTgg                                                                                                      <  1:7090667/150‑1 (MQ=255)

                                                   ttCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTa                                                                                                    <  1:3586050/150‑1 (MQ=255)

                                                    tctcGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTAc                                                                                                   >  2:4763969/1‑150 (MQ=255)

                                                     ctcGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACg                                                                                                  >  2:5489284/1‑150 (MQ=255)

                                                     ctcGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACg                                                                                                  <  1:8723215/150‑1 (MQ=255)

                                                        gTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCg                                                                                               >  2:3026889/1‑150 (MQ=255)

                                                        gTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCg                                                                                               >  1:8629740/1‑150 (MQ=255)

                                                            ttcATGTCGCCCTTGCGGATCATCGGGCCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTcc                                                                                           <  1:2853113/150‑1 (MQ=255)

                                                            ttcATGTCGCCCTTGCGGATCATCGGGCCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTcc                                                                                           >  2:28156/1‑150 (MQ=255)

                                                             tcATGTCGCCCTTGCGGATCATCGGGCCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCt                                                                                          >  1:780370/1‑150 (MQ=255)

                                                             tcATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCt                                                                                          >  1:6928452/1‑150 (MQ=255)

                                                              cATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTc                                                                                         <  1:3932907/150‑1 (MQ=255)

                                                                tGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGt                                                                                       <  2:7186849/150‑1 (MQ=255)

                                                                 ggCACCTTTGCGCACCACGGCGCCGGTTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTc                                                                                      <  1:2409581/150‑1 (MQ=255)

                                                                  gCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTcc                                                                                     <  1:1387432/150‑1 (MQ=255)

                                                                    aCCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCat                                                                                   >  1:1775272/1‑150 (MQ=255)

                                                                      cTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCatga                                                                                 <  2:675421/150‑1 (MQ=255)

                                                                             caccacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGAc                                                                          <  2:8233919/150‑1 (MQ=255)

                                                                              accacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGAcc                                                                         >  1:2067177/1‑150 (MQ=255)

                                                                                cacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCtt                                                                       >  2:318319/1‑150 (MQ=255)

                                                                                  cGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGa                                                                     <  1:2982548/150‑1 (MQ=255)

                                                                                   ggCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGAc                                                                    <  2:2033454/150‑1 (MQ=255)

                                                                                   ggCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGAc                                                                    <  2:4847103/150‑1 (MQ=255)

                                                                                    gcgcCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACg                                                                   <  2:7104740/150‑1 (MQ=255)

                                                                                     cgcCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACgg                                                                  <  2:2254973/150‑1 (MQ=255)

                                                                                     cgcCGGCTGCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCGCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACgg                                                                  <  2:6979654/150‑1 (MQ=255)

                                                                                       ccGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCGCGTCCATGATGCCGACCTTGACGgtg                                                                >  2:4206241/1‑150 (MQ=255)

                                                                                        cGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGgtgt                                                               >  2:4421/1‑150 (MQ=255)

                                                                                              ccATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGcgcg                                                         >  1:4126752/1‑150 (MQ=255)

                                                                                               cATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCgg                                                        <  2:1443805/150‑1 (MQ=255)

                                                                                                aTCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGc                                                       >  2:3704783/1‑150 (MQ=255)

                                                                                                   gAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATg                                                    <  2:2595986/150‑1 (MQ=255)

                                                                                                      gtgtGGATTTCATCGCCAGTGCGGTCCAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTGGAGGGTGTCCGGGGTACGGCGGGCCTCGGCCATGATGCCGCCCGTGACGGGGTTGCGCGGCagggcc                                                 >  2:7412655/1‑145 (MQ=255)

                                                                                                       tgtgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCca                                                >  2:9033215/1‑150 (MQ=255)

                                                                                                         tgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagc                                              >  2:2721453/1‑150 (MQ=255)

                                                                                                         tgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagc                                              <  1:5917872/150‑1 (MQ=255)

                                                                                                          ggATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagca                                             >  1:712562/1‑150 (MQ=255)

                                                                                                          ggATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagca                                             <  1:5221585/150‑1 (MQ=255)

                                                                                                           gATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCaagcag                                            >  2:8750164/1‑150 (MQ=255)

                                                                                                            aTTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCaagcagc                                           >  2:1960053/1‑149 (MQ=255)

                                                                                                            aTTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAgg                                           >  2:3791880/1‑150 (MQ=255)

                                                                                                               tCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCt                                        >  2:5304276/1‑150 (MQ=255)

                                                                                                                cATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCtt                                       <  2:4973172/150‑1 (MQ=255)

                                                                                                                 aTCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTc                                      >  1:6000299/1‑150 (MQ=255)

                                                                                                                  tCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCg                                     >  1:2360549/1‑150 (MQ=255)

                                                                                                                     ccAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGAcc                                  >  1:5393032/1‑150 (MQ=255)

                                                                                                                      cAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCt                                 <  2:3358335/150‑1 (MQ=255)

                                                                                                                      cAGTGCGGTCAAGGAAGCCGGTGCTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCt                                 <  2:6498823/150‑1 (MQ=255)

                                                                                                                      cAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCggtgc                                 <  1:3882097/150‑5 (MQ=255)

                                                                                                                       aGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTg                                <  1:1250613/150‑1 (MQ=255)

                                                                                                                        gTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGATGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                               <  1:6790293/150‑1 (MQ=255)

                                                                                                                        gTGCGGTCAAGGAAGCCGGTGATGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                               <  2:6072954/150‑1 (MQ=255)

                                                                                                                          gCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCGAAGACCTgctg                             <  1:6427725/150‑4 (MQ=255)

                                                                                                                           cGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTg                            <  2:5898222/150‑1 (MQ=255)

                                                                                                                            ggTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGa                           >  2:6308289/1‑150 (MQ=255)

                                                                                                                             gTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGaa                          >  1:845785/1‑150 (MQ=255)

                                                                                                                                 aGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtc                      >  1:2520769/1‑150 (MQ=255)

                                                                                                                                  ggAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtct                     >  2:6600664/1‑150 (MQ=255)

                                                                                                                                     gcgtggTGTTCATCAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCgg                  >  1:3125805/5‑150 (MQ=255)

                                                                                                                                       ccGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTg                <  2:5157206/150‑1 (MQ=255)

                                                                                                                                       ccGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTg                <  2:8468307/150‑1 (MQ=255)

                                                                                                                                        cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTgg               >  1:8217459/1‑150 (MQ=255)

                                                                                                                                        cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTgg               >  1:4828767/1‑150 (MQ=255)

                                                                                                                                          gtgtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCg             >  1:4439678/1‑150 (MQ=255)

                                                                                                                                           tgtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGa            <  1:4166241/150‑1 (MQ=255)

                                                                                                                                           tgtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGa            <  2:2117933/150‑1 (MQ=255)

                                                                                                                                            gtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGaa           <  1:5517572/150‑1 (MQ=255)

                                                                                                                                               gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGTACTAGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc        >  1:5156232/1‑150 (MQ=255)

                                                                                                                                               gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGt                 >  1:4936466/1‑141 (MQ=255)

                                                                                                                                               gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc        >  2:7891102/1‑150 (MQ=255)

                                                                                                                                               gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGAGAAGGc        >  2:7632811/1‑150 (MQ=255)

                                                                                                                                                atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGTAGGcc       >  2:8285742/1‑150 (MQ=255)

                                                                                                                                                atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGcc       >  1:7700592/1‑150 (MQ=255)

                                                                                                                                                atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGcc       <  2:5601638/150‑1 (MQ=255)

                                                                                                                                                atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGcc       >  1:156135/1‑150 (MQ=255)

                                                                                                                                                  gaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCAc     >  1:6645504/1‑150 (MQ=255)

                                                                                                                                                    acacCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCg                                     <  1:4299553/116‑1 (MQ=255)

                                                                                                                                                    acacCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACtt   <  2:16598/150‑1 (MQ=255)

CCGGTGGCCAGGCCAACGTCGACGTTGTTGTTTTCGTAGGCGCCGATCCACTTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTC  >  NC_002947/433698‑433996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`