BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,615,502	G→C	5.4%	E160D (GAG→GAC)	PP_4933 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,615,502	0	G	C	5.4%	931.2 / 30.5	275	E160D (GAG→GAC)	PP_4933	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (130/130); new base C (3/12); total (133/142)
Fisher's exact test for biased strand distribution p-value = 3.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 277 total aligned reads displayed.

CACACGGCCGTACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCC  >  NC_002947/5615353‑5615650

    cGGCCGTACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCg                                                                                                                                                   <  1:3046877/150‑1 (MQ=255)

       ccGTACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTg                                                                                                                                                <  1:964207/150‑1 (MQ=255)

        cGTACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGc                                                                                                                                               >  1:4090212/1‑150 (MQ=255)

         gTACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCACCGGACTCCGGAGcc                                                                                                                                              <  2:231583/150‑1 (MQ=255)

         gTACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCGTCAACGACACCTGGCTGCCCATCCCCGACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGcc                                                                                                                                              <  2:1679925/150‑1 (MQ=255)

          tACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGACGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCCGGCACCGGACTCCGGAGcca                                                                                                                                             <  1:8528765/150‑2 (MQ=255)

          tACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCCGGCACCGGACTCCGGAGcca                                                                                                                                             <  1:6513017/150‑2 (MQ=255)

           aCAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑Gc                                                                                                                                           >  2:1386952/1‑150 (MQ=255)

            cAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCa                                                                                                                                          >  1:2163840/1‑150 (MQ=255)

            cAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGC‑GGCACCGGACTCCGGAGccagca                                                                                                                                          <  1:6104545/150‑5 (MQ=255)

             aGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCCGGCACCGGACTCCGGAGccagca                                                                                                                                          <  2:4692604/150‑5 (MQ=255)

                aaGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGcc                                                                                                                                      <  1:4292717/150‑1 (MQ=255)

                aaGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGC‑GGCACCGGACTCCGGAGCCAGCACgaa                                                                                                                                      <  1:6918207/150‑3 (MQ=255)

                    ggCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGt                                                                                                                                  <  2:2940426/150‑1 (MQ=255)

                    ggCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGt                                                                                                                                  <  2:2674998/150‑1 (MQ=255)

                     gCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTg                                                                                                                                 >  1:6675174/1‑150 (MQ=255)

                       cGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCa                                                                                                                               >  1:9078975/1‑150 (MQ=255)

                       cGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCa                                                                                                                               >  2:8242833/1‑150 (MQ=255)

                        gaggagTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCAt                                                                                                                              >  2:5481948/1‑150 (MQ=255)

                         aggagTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCAtt                                                                                                                             >  2:523149/1‑150 (MQ=255)

                           gagTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGc                                                                                                                           <  1:5048675/150‑1 (MQ=255)

                            agTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCg                                                                                                                          <  2:956879/150‑1 (MQ=255)

                                  tgctgGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAAc                                                                                                                    >  2:5066484/1‑150 (MQ=255)

                                   gctgGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACt                                                                                                                   >  2:8636344/1‑150 (MQ=255)

                                     tgGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGa                                                                                                                 >  2:3392790/1‑150 (MQ=255)

                                     tgGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCAGTGCGGCAAACTGa                                                                                                                 >  2:2703150/1‑150 (MQ=255)

                                       gAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATg                                                                                                               <  1:7860660/150‑1 (MQ=255)

                                        aaaCCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGc                                                                                                              <  1:5732425/150‑1 (MQ=255)

                                           ccccGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTAc                                                                                                           <  2:6840558/150‑1 (MQ=255)

                                            cccGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACt                                                                                                          <  1:5522416/150‑1 (MQ=255)

                                             ccGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTg                                                                                                         >  2:1862723/1‑150 (MQ=255)

                                              cGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTgg                                                                                                        <  2:3690825/150‑1 (MQ=255)

                                               ggAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGa                                                                                                       <  2:7119281/150‑1 (MQ=255)

                                               ggAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGa                                                                                                       <  1:8425760/150‑1 (MQ=255)

                                                   ggTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAgcgc                                                                                                   <  1:5086484/150‑1 (MQ=255)

                                                    gTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGcc                                                                                                  <  2:6121575/150‑1 (MQ=255)

                                                         cTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACgg                                                                                             >  1:3726150/1‑150 (MQ=255)

                                                            ggcgggcgCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGcc                                                                                          >  1:443828/1‑150 (MQ=255)

                                                             gcgggcgCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCt                                                                                         <  1:2346507/150‑1 (MQ=255)

                                                              cgggcgCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACCCCCCCCTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTa                                                                                        <  1:522476/150‑1 (MQ=255)

                                                               gggcgCTTCAACGACTCCTGGCTGGCCATCCCCTACGCCGCCGCCCTTCACTACCGCGAAACTCCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACTCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTAt                                                                                       <  2:3729035/150‑1 (MQ=255)

                                                                     ttCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCAc                                                                                 <  1:8637304/150‑1 (MQ=255)

                                                                     ttCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCAc                                                                                 >  1:1388333/1‑150 (MQ=255)

                                                                     ttCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCAc                                                                                 >  2:4449317/1‑150 (MQ=255)

                                                                     ttCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCAc                                                                                 >  1:1782932/1‑150 (MQ=255)

                                                                       cAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCt                                                                               >  1:3598766/1‑150 (MQ=255)

                                                                        aaCGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTg                                                                              >  2:8344173/1‑150 (MQ=255)

                                                                         aCGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGc                                                                             >  2:4268992/1‑150 (MQ=255)

                                                                           gACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGccc                                                                           >  1:4710377/1‑150 (MQ=255)

                                                                            acacCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCa                                                                          <  1:8802992/150‑1 (MQ=255)

                                                                                cTGGCTGCCCCTCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGc                                                                      <  1:3876904/150‑1 (MQ=255)

                                                                                cTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGc                                                                      >  1:2196658/1‑150 (MQ=255)

                                                                                     tGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGc                                                                 <  1:1460339/150‑1 (MQ=255)

                                                                                       cccATCCCCGACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTa                                                               <  2:7580721/150‑1 (MQ=255)

                                                                                        ccATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTAc                                                              >  1:2903416/1‑150 (MQ=255)

                                                                                           tCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGc                                                           >  2:5817999/1‑150 (MQ=255)

                                                                                            ccccTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCt                                                          <  2:1873983/150‑1 (MQ=255)

                                                                                             cccTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTg                                                         >  2:2351309/1‑150 (MQ=255)

                                                                                              ccTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGa                                                        >  2:7399789/1‑150 (MQ=255)

                                                                                              ccTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGa                                                        >  2:2135261/1‑150 (MQ=255)

                                                                                              ccTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCAATGCGGCAAAATGATGCTACTGGAGAGCCCACCGGCCTATGTGCACCTGCCCACCGCTACGCTACAGCTGa                                                        >  2:6410212/1‑150 (MQ=255)

                                                                                                   gccgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTAt                                                   <  2:1779787/150‑1 (MQ=255)

                                                                                                   gccgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTAt                                                   >  1:2744515/1‑150 (MQ=255)

                                                                                                   gccgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTAt                                                   >  1:6533044/1‑150 (MQ=255)

                                                                                                   gccgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTAt                                                   <  2:335399/150‑1 (MQ=255)

                                                                                                    ccgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGTGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATg                                                  <  1:3375481/150‑1 (MQ=255)

                                                                                                    ccgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATg                                                  <  1:1916880/150‑1 (MQ=255)

                                                                                                     cgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGa                                                 <  1:2951688/150‑1 (MQ=255)

                                                                                                      gccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGAc                                                >  1:1266818/1‑150 (MQ=255)

                                                                                                         gccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATg                                             >  2:7433053/1‑150 (MQ=255)

                                                                                                         gccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATg                                             <  1:6871965/150‑1 (MQ=255)

                                                                                                                  tACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCaa                                    <  2:3952138/150‑1 (MQ=255)

                                                                                                                  tACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCaa                                    <  1:7150705/150‑1 (MQ=255)

                                                                                                                  tACCGCGAAACACCACACTTCGTGCTGGCTACGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCAACAGCTGATCTATGACATGCGCCTGCac                                    >  1:4946874/1‑149 (MQ=255)

                                                                                                                    ccGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGt                                  <  1:7437619/150‑1 (MQ=255)

                                                                                                                      gcgAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGAGCCTTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTCTGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGc                                >  2:8116703/1‑150 (MQ=255)

                                                                                                                        gAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGccc                              >  2:3634848/1‑150 (MQ=255)

                                                                                                                          aaCACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCg                            >  1:4220110/1‑150 (MQ=255)

                                                                                                                              ccccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGAc                        <  1:2031144/150‑1 (MQ=255)

                                                                                                                               cccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACg                       >  1:7309667/1‑150 (MQ=255)

                                                                                                                                ccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCTGGACGc                      <  2:5269917/150‑1 (MQ=255)

                                                                                                                                ccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGc                      <  1:7298485/150‑1 (MQ=255)

                                                                                                                                ccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGc                      >  1:5568083/1‑150 (MQ=255)

                                                                                                                                ccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGc                      >  2:7956569/1‑150 (MQ=255)

                                                                                                                                   cTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCaa                   <  1:2538363/150‑1 (MQ=255)

                                                                                                                                   cTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCaa                   <  1:8202717/150‑1 (MQ=255)

                                                                                                                                     tCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCAGTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGa                 <  2:3081539/150‑1 (MQ=255)

                                                                                                                                     ggcgtgcggCACCGGACTCCGGAGCCAGCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAg                  >  1:8598914/8‑150 (MQ=255)

                                                                                                                                       gTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGaaa               <  2:4501237/150‑1 (MQ=255)

                                                                                                                                       gTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGaaa               <  1:3641528/150‑1 (MQ=255)

                                                                                                                                        tGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAAc              >  1:1121316/1‑150 (MQ=255)

                                                                                                                                        tGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAAc              >  1:6571458/1‑150 (MQ=255)

                                                                                                                                        tGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAAc              <  2:4190771/150‑1 (MQ=255)

                                                                                                                                           tGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCa           >  1:586770/1‑150 (MQ=255)

                                                                                                                                           tGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCa           <  1:8304137/150‑1 (MQ=255)

                                                                                                                                           tGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCa           >  2:1025251/1‑150 (MQ=255)

                                                                                                                                              cTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCt        <  2:903441/150‑1 (MQ=255)

                                                                                                                                               tCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATgc                                            >  1:4467367/1‑113 (MQ=255)

                                                                                                                                               tCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCtt       >  1:309719/1‑150 (MQ=255)

                                                                                                                                                    aGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTcc  <  2:6523803/150‑1 (MQ=255)

CACACGGCCGTACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCC  >  NC_002947/5615353‑5615650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`