BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,737,927	C→G	6.0%	S499S (TCG→TCC)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,737,927	0	C	G	6.0%	890.9 / 33.1	268	S499S (TCG→TCC)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base C (87/164); new base G (7/9); total (95/173)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 291 total aligned reads displayed.

CCCTGCTCCTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAC  >  NC_002947/5737778‑5738076

 ccTGCTCCTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCg                                                                                                                                                      >  2:7986159/1‑150 (MQ=255)

     cTCCTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGaa                                                                                                                                                  <  2:9153483/150‑1 (MQ=255)

     cTCCTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGaa                                                                                                                                                  <  2:4500064/150‑1 (MQ=255)

     cTCCTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGaa                                                                                                                                                  <  1:3824620/150‑1 (MQ=255)

       ccTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGc                                                                                                                                                <  1:6734144/150‑1 (MQ=255)

        cTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGcc                                                                                                                                               >  2:5917853/1‑150 (MQ=255)

         ttGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGccc                                                                                                                                              <  2:3377932/150‑1 (MQ=255)

             cgcAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGc                                                                                                                                          <  1:9067624/150‑1 (MQ=255)

              gcAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGcc                                                                                                                                         >  1:8039525/1‑150 (MQ=255)

                    gATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGcc                                                                                                                                   >  1:8465353/1‑150 (MQ=255)

                      tGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCAc                                                                                                                                 <  1:5631144/150‑1 (MQ=255)

                           aTGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCa                                                                                                                            <  1:3939535/150‑1 (MQ=255)

                             gTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCTAGAAGCCCATGCCCACGCCACCGCCATg                                                                                                                          <  2:8683406/150‑1 (MQ=255)

                             gTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATg                                                                                                                          >  2:6371657/1‑150 (MQ=255)

                             gTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATg                                                                                                                          <  2:1359585/150‑1 (MQ=255)

                              tCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATgg                                                                                                                         <  2:4876178/150‑1 (MQ=255)

                              tCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTAGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATgg                                                                                                                         >  2:2039034/1‑150 (MQ=255)

                                 tAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCAGCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCCCGCCACCGCCAGGGTGc                                                                                                                      >  2:5701651/1‑150 (MQ=255)

                                  aaCCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCa                                                                                                                     <  1:6632408/150‑1 (MQ=255)

                                    ccGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGc                                                                                                                   >  2:7557249/1‑150 (MQ=255)

                                    ccGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGc                                                                                                                   <  2:1901998/150‑1 (MQ=255)

                                    ccGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGc                                                                                                                   <  1:2894225/150‑1 (MQ=255)

                                           cGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATAccc                                                                                                            <  1:2390169/150‑1 (MQ=255)

                                                 gACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGtgctgc                                                                                                                                                       >  1:4452364/1‑101 (MQ=255)

                                                 gACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTgg                                                                                                      >  1:5783160/1‑150 (MQ=255)

                                                    gCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGgcgc                                                                                                   >  1:1580495/1‑150 (MQ=255)

                                                    gCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGgcgc                                                                                                   >  1:5236799/1‑150 (MQ=255)

                                                    gCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGgcgc                                                                                                   >  1:6243929/1‑150 (MQ=255)

                                                     cATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGcgcc                                                                                                  <  1:3264651/150‑1 (MQ=255)

                                                     cATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGcgcc                                                                                                  <  1:8224887/150‑1 (MQ=255)

                                                     cATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATG‑CCACGCCACCGCCATGGTGCAg                                                                                                                    <  1:8939663/131‑1 (MQ=255)

                                                      aTGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGcgccg                                                                                                 >  1:7600190/1‑150 (MQ=255)

                                                         aCGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCt                                                                                              <  1:5062659/150‑1 (MQ=255)

                                                            ccAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCg                                                                                           <  2:8902801/150‑1 (MQ=255)

                                                                  ccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTc                                                                                     <  2:7014118/150‑1 (MQ=255)

                                                                  ccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTc                                                                                     <  1:4586538/150‑1 (MQ=255)

                                                                   cTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcc                                                                                    >  2:3422476/1‑149 (MQ=255)

                                                                   cTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTca                                                                                    >  2:1679318/1‑150 (MQ=255)

                                                                     gggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                  <  1:7075967/150‑1 (MQ=255)

                                                                     gggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                  >  2:5756310/1‑150 (MQ=255)

                                                                      ggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCATGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagca                                                                                 >  1:8090504/1‑150 (MQ=255)

                                                                         cGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAggg                                                                              <  1:2257951/150‑1 (MQ=255)

                                                                          gTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGc                                                                             <  2:6766879/150‑1 (MQ=255)

                                                                          gTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGc                                                                             >  1:3039777/1‑150 (MQ=255)

                                                                             ggTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGtt                                                                          <  2:3276648/150‑1 (MQ=255)

                                                                             ggTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGtt                                                                          <  1:5985719/150‑1 (MQ=255)

                                                                             ggTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGtt                                                                          <  2:8618895/150‑1 (MQ=255)

                                                                              gTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTg                                                                         <  1:2564506/150‑1 (MQ=255)

                                                                               tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                        <  2:1148111/150‑1 (MQ=255)

                                                                               tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                        <  2:7934016/150‑1 (MQ=255)

                                                                               tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                        >  1:7657476/1‑150 (MQ=255)

                                                                                  gTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagca                                                                     <  1:6371657/150‑1 (MQ=255)

                                                                                    aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                   <  2:7328920/150‑1 (MQ=255)

                                                                                    aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                   <  1:6121518/150‑1 (MQ=255)

                                                                                    aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                   <  2:382438/150‑1 (MQ=255)

                                                                                     cccGGGCGATGCGCTCGGCGGCCTCATCGGTCCCGTCGCAGCCGAGGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCAGGGTGCAGCGATACCCCGGTGGCGCCGCCGGCGGTGTTCAGCAGGGCGTTGAGCAGCg                                                                  >  1:5246045/1‑150 (MQ=255)

                                                                                      ccGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCgg                                                                 <  2:267292/150‑1 (MQ=255)

                                                                                       cGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGc                                                                >  2:5986859/1‑150 (MQ=255)

                                                                                         ggCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCa                                                              <  2:4783124/150‑1 (MQ=255)

                                                                                            gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                           <  2:4719174/150‑1 (MQ=255)

                                                                                            gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                           <  2:1782927/150‑1 (MQ=255)

                                                                                            gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                           <  2:8985040/150‑1 (MQ=255)

                                                                                            gATGCGCTCGGCGGCCTCATCGGGACCGTCGCCGACGATGACCAGGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCCGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                           <  2:3677024/150‑1 (MQ=255)

                                                                                               gcgcTGGTCGGCATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGa                                                        <  1:4150559/150‑1 (MQ=255)

                                                                                                 gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                      >  1:3796765/1‑150 (MQ=255)

                                                                                                 gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                      <  2:2424623/150‑1 (MQ=255)

                                                                                                      tcggcATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCg                                                 >  2:2892170/2‑150 (MQ=255)

                                                                                                           cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                            >  1:2774908/1‑150 (MQ=255)

                                                                                                            tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                           >  2:6171025/1‑150 (MQ=255)

                                                                                                              ttccgaaCCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGcc                                         >  1:1125367/7‑150 (MQ=255)

                                                                                                              aTCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGGGAAGCCCATGCCCACGCCACCGCCATGGTGCAg                                                                                                                    <  2:3110702/75‑1 (MQ=255)

                                                                                                                 ggTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATc                                      <  1:6592507/150‑1 (MQ=255)

                                                                                                                  gaaCCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTct                                     >  1:1702695/3‑149 (MQ=255)

                                                                                                                  gaaCCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTct                                     >  1:8831925/3‑149 (MQ=255)

                                                                                                                    aCCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACg                                   <  2:3783721/150‑1 (MQ=255)

                                                                                                                        tCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATg                               <  1:3588422/150‑1 (MQ=255)

                                                                                                                           cAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCGCCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGcc                            >  2:3531098/1‑150 (MQ=255)

                                                                                                                           cAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGcc                            >  2:5320522/1‑150 (MQ=255)

                                                                                                                             gACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTc                          >  1:6716450/1‑150 (MQ=255)

                                                                                                                              aCGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCg                         <  2:4353302/150‑1 (MQ=255)

                                                                                                                                gATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGt                       >  2:507378/1‑150 (MQ=255)

                                                                                                                                 aTGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGtt                      <  1:5414446/150‑1 (MQ=255)

                                                                                                                                 aTGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTCTTTCATGGATTCGGtt                      >  1:4856852/1‑150 (MQ=255)

                                                                                                                                     ccATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgc                  >  2:1973632/1‑150 (MQ=255)

                                                                                                                                     ccATGCCAGAGCGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGAGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTGTcgc                  >  2:3880671/1‑150 (MQ=255)

                                                                                                                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                 >  1:8575759/1‑150 (MQ=255)

                                                                                                                                           ccGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCg            >  2:547738/1‑150 (MQ=255)

                                                                                                                                           ccGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCg            <  2:2540543/150‑1 (MQ=255)

                                                                                                                                           ccGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTCTTTCATGGATTCGGTTTCACGGtttg            <  1:4253139/150‑3 (MQ=255)

                                                                                                                                             gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg          >  1:1327361/1‑150 (MQ=255)

                                                                                                                                             gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg          <  1:2107994/150‑1 (MQ=255)

                                                                                                                                               gTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCt        <  2:8282390/150‑1 (MQ=255)

                                                                                                                                                tgctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTg       <  2:3955709/150‑1 (MQ=255)

                                                                                                                                                tgctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTg       <  2:3319304/150‑1 (MQ=255)

                                                                                                                                                tgctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTg       <  1:3363576/150‑1 (MQ=255)

                                                                                                                                                 gctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTgg      <  1:370538/150‑1 (MQ=255)

                                                                                                                                                   tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt    >  1:2649802/1‑150 (MQ=255)

                                                                                                                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc  <  1:2039034/150‑1 (MQ=255)

                                                                                                                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc  >  2:1891638/1‑150 (MQ=255)

                                                                                                                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc  <  1:7950319/150‑1 (MQ=255)

CCCTGCTCCTTGGCGCAGTCGATGGCGATGTCATAACCGGCATCGGCGTGACGCATGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAC  >  NC_002947/5737778‑5738076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`