BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,737,982	C→G	6.5%	G481A (GGC→GCC) ‡	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,737,982	0	C	G	6.5%	933.0 / 39.5	278	G481A (GGC→GCC) ‡	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base C (111/149); new base G (9/9); total (120/158)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 289 total aligned reads displayed.

TGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCG  >  NC_002947/5737833‑5738131

 gACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCgccgcc                                                                                                                                                      >  2:517174/1‑150 (MQ=255)

  aCGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCt                                                                                                                                                     <  1:5062659/150‑1 (MQ=255)

    gCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGCGGCGCCACCTgg                                                                                                                                                   <  1:2102979/150‑2 (MQ=255)

     ccAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCg                                                                                                                                                  <  2:335895/150‑1 (MQ=255)

     ccAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCg                                                                                                                                                  <  2:868296/150‑1 (MQ=255)

       aGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGgt                                                                                                                                                >  1:6766879/1‑150 (MQ=255)

          cccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGtt                                                                                                                                             <  1:300807/150‑1 (MQ=255)

           ccTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTc                                                                                                                                            <  1:1614689/150‑1 (MQ=255)

            cTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcc                                                                                                                                           >  2:3422476/1‑149 (MQ=255)

            cTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTca                                                                                                                                           >  2:1679318/1‑150 (MQ=255)

              gggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                                                                         <  1:7075967/150‑1 (MQ=255)

              gggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagc                                                                                                                                         <  1:4837222/150‑1 (MQ=255)

               ggTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTcagca                                                                                                                                        >  2:5712107/1‑150 (MQ=255)

                  cGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAggg                                                                                                                                     <  1:2257951/150‑1 (MQ=255)

                   gTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGc                                                                                                                                    >  2:5812313/1‑150 (MQ=255)

                   gTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGc                                                                                                                                    <  2:6766879/150‑1 (MQ=255)

                      ggTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGtt                                                                                                                                 <  2:8618895/150‑1 (MQ=255)

                       gTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTg                                                                                                                                <  1:2564506/150‑1 (MQ=255)

                        tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                                                               >  1:7657476/1‑150 (MQ=255)

                        tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                                                               <  2:1323531/150‑1 (MQ=255)

                        tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                                                               <  2:1961702/150‑1 (MQ=255)

                        tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                                                               <  2:80401/150‑1 (MQ=255)

                        tCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGa                                                                                                                               <  2:7934016/150‑1 (MQ=255)

                           gTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagca                                                                                                                            <  1:6371657/150‑1 (MQ=255)

                             aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                                                                          <  2:382438/150‑1 (MQ=255)

                             aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                                                                          <  2:3522263/150‑1 (MQ=255)

                             aCCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGagcagc                                                                                                                          <  1:281657/150‑1 (MQ=255)

                              cccGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCg                                                                                                                         <  2:6957662/150‑1 (MQ=255)

                               ccGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCgg                                                                                                                        <  2:2505344/150‑1 (MQ=255)

                               ccGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCgg                                                                                                                        <  2:267048/150‑1 (MQ=255)

                                cGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGc                                                                                                                       >  2:5986736/1‑150 (MQ=255)

                                cGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGc                                                                                                                       >  1:6207454/1‑150 (MQ=255)

                                cGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGc                                                                                                                       >  1:6709994/1‑150 (MQ=255)

                                   gCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGtt                                                                                                                                             <  1:5750213/125‑1 (MQ=255)

                                    cGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGt                                                                                                                   <  1:3436192/150‑1 (MQ=255)

                                     gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                                                                                  <  2:8985040/150‑1 (MQ=255)

                                     gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                                                                                  >  2:300479/1‑150 (MQ=255)

                                     gATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                                                                                  <  2:1782927/150‑1 (MQ=255)

                                     gATGCGCTCGGCGGCCTCATCGGGACCGTCGCCGACGATGACCAGGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCCGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTc                                                                                                                  <  2:3677024/150‑1 (MQ=255)

                                        gcgcTGGTCGGCATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGa                                                                                                               <  1:4150559/150‑1 (MQ=255)

                                         cgcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaa                                                                                                              <  1:3603490/150‑1 (MQ=255)

                                          gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                             <  2:3289090/150‑1 (MQ=255)

                                          gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                             >  1:7975173/1‑150 (MQ=255)

                                          gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                             <  2:2424623/150‑1 (MQ=255)

                                            tCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACa                                                                                                           <  2:2003268/150‑1 (MQ=255)

                                            tCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACa                                                                                                           >  1:4575131/1‑150 (MQ=255)

                                               tcggcATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCg                                                                                                        <  2:7390652/149‑1 (MQ=255)

                                               tcggcATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCg                                                                                                        >  2:2892170/2‑150 (MQ=255)

                                                    cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                                                                                   >  1:2774908/1‑150 (MQ=255)

                                                     tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                                                                  >  1:8855216/1‑150 (MQ=255)

                                                     tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                                                                  >  2:6171025/1‑150 (MQ=255)

                                                       ttccgaaCCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGcc                                                                                                >  1:1125367/7‑150 (MQ=255)

                                                          ggTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATc                                                                                             <  1:6592507/150‑1 (MQ=255)

                                                             aCCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACg                                                                                          <  2:3783721/150‑1 (MQ=255)

                                                                 tCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATg                                                                                      <  1:5238821/150‑1 (MQ=255)

                                                                    cAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGcc                                                                                   >  2:5320522/1‑150 (MQ=255)

                                                                    cAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGcc                                                                                   >  1:6063619/1‑150 (MQ=255)

                                                                      gACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTc                                                                                 <  2:1113309/150‑1 (MQ=255)

                                                                              ccATGCCAGAGCGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGAGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTGTcgc                                                                         >  2:3880671/1‑150 (MQ=255)

                                                                               cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                        >  1:8575759/1‑150 (MQ=255)

                                                                               cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                        >  1:3321875/1‑150 (MQ=255)

                                                                                aTGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCgg                                                                       <  2:611073/150‑1 (MQ=255)

                                                                                aTGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCgg                                                                       <  2:8817816/150‑1 (MQ=255)

                                                                                    ccGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCg                                                                   >  2:547738/1‑150 (MQ=255)

                                                                                    ccGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCGTCTTTCATGGATTCGGTTTCACGGtttg                                                                   <  1:4253139/150‑3 (MQ=255)

                                                                                      gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg                                                                 >  1:1327361/1‑150 (MQ=255)

                                                                                         tgctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTg                                                              <  2:3955709/150‑1 (MQ=255)

                                                                                         tgctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTg                                                              <  2:4292017/150‑1 (MQ=255)

                                                                                          gctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTgg                                                             <  1:370538/150‑1 (MQ=255)

                                                                                           ctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGa                                                            <  2:6321134/150‑1 (MQ=255)

                                                                                            tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                                                           >  1:2649802/1‑150 (MQ=255)

                                                                                              cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                         >  2:3273027/1‑150 (MQ=255)

                                                                                              cGAGAAGCCAATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                         <  1:2139568/150‑1 (MQ=255)

                                                                                              cGAGAAGCCAATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                         <  1:5479970/150‑1 (MQ=255)

                                                                                               gagaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAcc                                                        >  1:1562673/1‑150 (MQ=255)

                                                                                                     cccATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCg                                                  >  2:8298542/1‑150 (MQ=255)

                                                                                                     cccATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCg                                                  >  1:4034197/1‑150 (MQ=255)

                                                                                                      ccATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCgg                                                 >  2:4818269/1‑150 (MQ=255)

                                                                                                      ccATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCgg                                                 >  1:5279505/1‑150 (MQ=255)

                                                                                                       cATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGa                                                >  2:9046513/1‑150 (MQ=255)

                                                                                                       cATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGa                                                >  1:3826714/1‑150 (MQ=255)

                                                                                                        aTGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAg                                               <  2:2711990/150‑1 (MQ=255)

                                                                                                        aTGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAg                                               >  1:7801196/1‑150 (MQ=255)

                                                                                                            ccACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGGCGGACACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGa                                           >  2:4570828/1‑150 (MQ=255)

                                                                                                             cACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAg                                          <  2:4915556/150‑1 (MQ=255)

                                                                                                                 ccACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggtgg                                      >  1:7008654/1‑150 (MQ=255)

                                                                                                                   aCCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTc                                    >  2:1876243/1‑150 (MQ=255)

                                                                                                                   aCCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTc                                    <  2:4034197/150‑1 (MQ=255)

                                                                                                                        cATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGc                               >  1:4167685/1‑150 (MQ=255)

                                                                                                                        cATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGc                               >  2:7535023/1‑150 (MQ=255)

                                                                                                                          tGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCg                             <  2:70567/150‑1 (MQ=255)

                                                                                                                            gTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGAt                           <  1:907215/150‑1 (MQ=255)

                                                                                                                                aGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATcacc                       >  1:8218306/1‑150 (MQ=255)

                                                                                                                                 gCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATcacca                      >  1:5663353/1‑150 (MQ=255)

                                                                                                                                      aCCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGt                 <  2:4750855/150‑1 (MQ=255)

                                                                                                                                        ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTc                                    <  2:7553899/129‑1 (MQ=255)

                                                                                                                                        ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTc                                    >  1:7553899/1‑129 (MQ=255)

                                                                                                                                             tGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACa          <  1:5885824/150‑1 (MQ=255)

                                                                                                                                                 gccgccTGTGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATTGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc      >  2:1512625/1‑150 (MQ=255)

                                                                                                                                                 gccgccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc      >  2:4458257/1‑150 (MQ=255)

TGACGCCAGTCCCTGGGTCGTTGGTCAGTACCCGGGCGATGCGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCG  >  NC_002947/5737833‑5738131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`