BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,738,023	A→G	5.7%	V467V (GTT→GTC)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,738,023	0	A	G	5.7%	780.3 / 22.0	245	V467V (GTT→GTC)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base A (128/102); new base G (9/5); total (138/107)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 265 total aligned reads displayed.

CGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGGCGCGA  >  NC_002947/5737874‑5738173

cgcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaa                                                                                                                                                        <  1:6625023/150‑1 (MQ=255)

 gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                                                                       >  1:3796765/1‑150 (MQ=255)

 gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                                                                       <  2:2425153/150‑1 (MQ=255)

 gcTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGaaa                                                                                                                                                       <  2:7051159/150‑1 (MQ=255)

   tCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACa                                                                                                                                                     <  2:2003268/150‑1 (MQ=255)

      tcggcATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCg                                                                                                                                                  <  2:7390652/149‑1 (MQ=255)

      tcggcATCTTCCGAACCGTCGCAGCAGATGACCATGCCCGAATGTTGGGAGAAGCCCATGCCGACGCCGCCACCATGATGGAGCGATACCCATGTCGCACCCGATGCGGTGTTGAGCAACGCATTGAGCAGCGGCCAGTCGGAGACTGCg                                                                                                                                                  >  2:2892307/2‑150 (MQ=255)

        ggcCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGCTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCggc                                                                                                                                                >  2:3824794/1‑148 (MQ=255)

           cTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGa                                                                                                                                             >  1:2774908/1‑150 (MQ=255)

            tCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGaa                                                                                                                                            <  1:5346000/150‑1 (MQ=255)

                 ggTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATc                                                                                                                                       <  2:8776183/150‑1 (MQ=255)

                    aCCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACg                                                                                                                                    <  2:3783721/150‑1 (MQ=255)

                       gTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCAt                                                                                                                                 <  2:7978908/150‑1 (MQ=255)

                         cGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATgg                                                                                                                               <  2:3765858/150‑1 (MQ=255)

                           cAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCGCCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGcc                                                                                                                             >  2:3531098/1‑150 (MQ=255)

                                gATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGt                                                                                                                        >  1:2500874/1‑150 (MQ=255)

                                gATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGt                                                                                                                        >  2:507378/1‑150 (MQ=255)

                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                                                                  >  1:3321875/1‑150 (MQ=255)

                                      cATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTcgcg                                                                                                                  >  1:8575759/1‑150 (MQ=255)

                                           ccGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCg                                                                                                             >  2:547738/1‑150 (MQ=255)

                                             gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg                                                                                                           <  1:2107970/150‑1 (MQ=255)

                                             gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg                                                                                                           >  1:1327361/1‑150 (MQ=255)

                                             gAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCggg                                                                                                           <  1:4903663/150‑1 (MQ=255)

                                                tgctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTg                                                                                                        <  2:3319304/150‑1 (MQ=255)

                                                tgctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTg                                                                                                        <  2:3955709/150‑1 (MQ=255)

                                                  ctgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGa                                                                                                      <  2:6321134/150‑1 (MQ=255)

                                                   tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                                                                                                     >  1:2649802/1‑150 (MQ=255)

                                                   tgcGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGAt                                                                                                     >  1:1575028/1‑150 (MQ=255)

                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                                                                   >  2:1891638/1‑150 (MQ=255)

                                                     cGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                                                                   <  1:7950319/150‑1 (MQ=255)

                                                     cGAGAAGCCAATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                                                                   <  1:5479970/150‑1 (MQ=255)

                                                     cGAGAAGCCAATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATAc                                                                                                   <  1:2139572/150‑1 (MQ=255)

                                                        gaAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGa                                                                                                >  2:1599766/1‑150 (MQ=255)

                                                           gCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGcc                                                                                             <  1:3422476/150‑1 (MQ=255)

                                                            cccATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCg                                                                                            >  2:8298542/1‑150 (MQ=255)

                                                             ccATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCgg                                                                                           >  1:5279505/1‑150 (MQ=255)

                                                             ccATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCgg                                                                                           >  2:4818269/1‑150 (MQ=255)

                                                              cATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGa                                                                                          >  2:9046513/1‑150 (MQ=255)

                                                              cATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGa                                                                                          >  1:6835043/1‑150 (MQ=255)

                                                               aTGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAg                                                                                         >  1:7801196/1‑150 (MQ=255)

                                                               aTGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAg                                                                                         >  2:6133012/1‑150 (MQ=255)

                                                                   ccACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGa                                                                                     >  1:6405057/1‑150 (MQ=255)

                                                                    cACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAg                                                                                    <  1:7986159/150‑1 (MQ=255)

                                                                    cACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAg                                                                                    >  2:8378699/1‑150 (MQ=255)

                                                                    cACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAg                                                                                    <  2:2592660/150‑1 (MQ=255)

                                                                      cGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggt                                                                                  <  1:3689870/150‑1 (MQ=255)

                                                                      cGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGAGGACAGTCGGAAACATCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggt                                                                                  <  1:5787249/150‑1 (MQ=255)

                                                                        ccACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAggtgg                                                                                >  1:7008654/1‑150 (MQ=255)

                                                                         cgccgccgTGATGGAGTGACACCCACGTTGCGCCCGAAGCGGTGTTGAGCAGAGCATTGAGCAGCGGCCAGTCGGAGACTGCGTCCGAGCCATCCTTCATGGCTTCCGTTTCGCGGTTCGGCGAAGCCACCGAGCCGCTATCGAggtgat                                                                               >  2:6880071/3‑148 (MQ=255)

                                                                          aCCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTc                                                                              <  2:4034197/150‑1 (MQ=255)

                                                                            cGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCAc                                                                            >  2:4895058/1‑150 (MQ=255)

                                                                               cATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGc                                                                         >  1:4167685/1‑150 (MQ=255)

                                                                               cATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGc                                                                         >  1:8960879/1‑150 (MQ=255)

                                                                                   gTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGAt                                                                     >  1:8385533/1‑150 (MQ=255)

                                                                                        gCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATcacca                                                                >  1:5663353/1‑150 (MQ=255)

                                                                                               ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTc                                                                              >  1:7553899/1‑129 (MQ=255)

                                                                                               ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGc                                                         >  1:6193735/1‑150 (MQ=255)

                                                                                               ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGc                                                         >  1:3995982/1‑150 (MQ=255)

                                                                                               ccAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGc                                                         >  1:1130957/1‑150 (MQ=255)

                                                                                                cAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGcc                                                        >  1:3741461/1‑150 (MQ=255)

                                                                                                 aGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCg                                                       >  1:1556159/1‑150 (MQ=255)

                                                                                                      gcgcCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGc                                                  >  2:7537716/1‑150 (MQ=255)

                                                                                                        gccgccTGTGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATTGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc                                                >  2:1512625/1‑150 (MQ=255)

                                                                                                        gccgccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc                                                >  2:7504058/1‑150 (MQ=255)

                                                                                                        gccgccTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTc                                                >  2:4458257/1‑150 (MQ=255)

                                                                                                               gCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCAGGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTcgcccggg                                         <  2:5246045/150‑5 (MQ=255)

                                                                                                                 ggTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCg                                       >  2:6539748/1‑150 (MQ=255)

                                                                                                                   tgtTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGa                                     <  2:4575131/150‑1 (MQ=255)

                                                                                                                     ttCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGAcc                                   >  1:7345953/1‑150 (MQ=255)

                                                                                                                      tCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCa                                  >  2:6021683/1‑150 (MQ=255)

                                                                                                                        agcagGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCAtt                                >  1:4129637/1‑150 (MQ=255)

                                                                                                                        agcagGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCAtt                                >  1:4452043/1‑150 (MQ=255)

                                                                                                                         gcagGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCAttt                               >  2:704860/1‑150 (MQ=255)

                                                                                                                         gcagGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCAttt                               >  2:3403960/1‑150 (MQ=255)

                                                                                                                           agGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCg                             <  1:5917853/150‑1 (MQ=255)

                                                                                                                            gggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATcaccac                                                               <  1:4285175/115‑1 (MQ=255)

                                                                                                                            gggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATcaccac                                                               >  2:4285175/1‑115 (MQ=255)

                                                                                                                             ggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCATCGGTGCCGAAAGCTCGCCGCTGCGGACCATTTCGtt                           <  1:2866790/150‑1 (MQ=255)

                                                                                                                             ggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGtt                           <  2:5236799/150‑1 (MQ=255)

                                                                                                                             ggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGtt                           <  2:6243929/150‑1 (MQ=255)

                                                                                                                             ggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGtt                           <  2:8215390/150‑1 (MQ=255)

                                                                                                                             ggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGtt                           <  1:1973632/150‑1 (MQ=255)

                                                                                                                             ggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGtt                           <  2:3039777/150‑1 (MQ=255)

                                                                                                                             ggCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGtt                           <  2:7801196/150‑1 (MQ=255)

                                                                                                                              gCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTg                          <  2:6709994/150‑1 (MQ=255)

                                                                                                                               cGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGa                         >  2:3120812/1‑150 (MQ=255)

                                                                                                                               cGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGa                         <  2:1327361/150‑1 (MQ=255)

                                                                                                                                gTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGaa                        <  1:307917/150‑1 (MQ=255)

                                                                                                                                 ttGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGaaa                       >  1:970754/1‑150 (MQ=255)

                                                                                                                                     gcagcGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCa                   <  2:2774908/150‑1 (MQ=255)

                                                                                                                                       agcGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAgg                 <  2:7657320/150‑1 (MQ=255)

                                                                                                                                           gCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCa             >  2:434495/1‑150 (MQ=255)

                                                                                                                                                tCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTg        <  1:5701651/150‑1 (MQ=255)

                                                                                                                                                tCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTg        <  2:5783160/150‑1 (MQ=255)

                                                                                                                                                  ggAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGgc      <  1:1100300/150‑1 (MQ=255)

                                                                                                                                                    aaaCAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGGGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGgcgc    >  1:2532570/1‑150 (MQ=255)

                                                                                                                                                    aaaCAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGgcgc    >  1:877706/1‑150 (MQ=255)

                                                                                                                                                    aaaCAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGgcgc    >  2:8953742/1‑150 (MQ=255)

                                                                                                                                                    aaaCAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGgcgc    >  2:8953903/1‑150 (MQ=255)

                                                                                                                                                     aaCAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAG‑CTGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGGCGCGa  >  2:2342274/1‑150 (MQ=255)

CGCTCGGCGGCCTCATCGGTACCGTCGCAGACGATGACCATGCCCGAGTGCTGCGAGAAGCCCATGCCCACGCCACCGCCATGGTGCAGCGATACCCAGGTGGCGCCGCCTGCGGTGTTCAGCAGGGCGTTGAGCAGCGGCCAGTCGGAAACAGCGTCCGAACCATCACGCATGGCCTCGGTTTCGCGGTTCGGGCTGGATACCGAGCCGGAGTCGAGGTGGTCACGGCCGATCACCACCGGTGCCGACAGCTCGCCGCTGCGGACCATTTCGTTGAAAGCCAGGCCCAGCTTGGCGCGA  >  NC_002947/5737874‑5738173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`