BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,738,911	G→C	10.1%	L171L (CTC→CTG)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,738,911	0	G	C	10.1%	977.7 / 78.0	306	L171L (CTC→CTG)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base G (115/159); new base C (7/24); total (122/184)
Fisher's exact test for biased strand distribution p-value = 5.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 307 total aligned reads displayed.

CGTCGAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTC  >  NC_002947/5738763‑5739060

cgtcgAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGa                                                                                                                                                      <  1:1419574/150‑1 (MQ=255)

cgtcgAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGa                                                                                                                                                      <  2:1740488/150‑1 (MQ=255)

 gtcgAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAg                                                                                                                                                     <  2:568861/150‑1 (MQ=255)

 gtcgAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAg                                                                                                                                                     >  1:4400871/1‑150 (MQ=255)

 gtcgAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAg                                                                                                                                                     >  1:4991762/1‑150 (MQ=255)

    gAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTAc                                                                                                                                                  >  2:799805/1‑150 (MQ=255)

    gAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTAc                                                                                                                                                  <  2:8272379/150‑1 (MQ=255)

          aGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACtt                                                                                                                                            <  2:4622937/150‑1 (MQ=255)

              gCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCt                                                                                                                                        >  2:4810287/1‑150 (MQ=255)

               ccTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCGGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCtt                                                                                                                                       <  1:2096013/150‑1 (MQ=255)

                 tGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGATGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTc                                                                                                                                     >  1:6988153/1‑150 (MQ=255)

                  gCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCa                                                                                                                                    <  1:5594008/150‑1 (MQ=255)

                  gCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCa                                                                                                                                    <  2:6362102/150‑1 (MQ=255)

                      gtcgACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCt                                                                                                                                <  2:5424792/150‑1 (MQ=255)

                      gtcgACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCt                                                                                                                                >  1:3138112/1‑150 (MQ=255)

                      gtcgACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCt                                                                                                                                >  2:7809163/1‑150 (MQ=255)

                               gCGGGTTTCCTGGCGGTTGTCGATACGGCTCTGCGGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTa                                                                                                                       <  1:4581569/150‑1 (MQ=255)

                               gCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTa                                                                                                                       <  2:1670314/150‑1 (MQ=255)

                                 gggTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGt                                                                                                                     >  2:3851752/1‑150 (MQ=255)

                                  ggTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTg                                                                                                                    <  1:1873557/150‑1 (MQ=255)

                                    tttCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCt                                                                                                                  >  1:5479964/1‑150 (MQ=255)

                                    tttCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCt                                                                                                                  <  2:2931629/150‑1 (MQ=255)

                                    tttCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCt                                                                                                                  <  2:8637410/150‑1 (MQ=255)

                                      tCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTgg                                                                                                                >  1:1321041/1‑150 (MQ=255)

                                        cAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCg                                                                                                              >  2:1621628/1‑150 (MQ=255)

                                         aGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGa                                                                                                             >  2:9086176/1‑150 (MQ=255)

                                           gCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGAcc                                                                                                           <  1:7213880/150‑1 (MQ=255)

                                                 gTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTc                                                                                                     <  1:3430814/150‑1 (MQ=255)

                                                      tACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTcgacga                                                                                                >  2:1312383/1‑150 (MQ=255)

                                                        cGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAg                                                                                              <  1:7717565/150‑1 (MQ=255)

                                                        cGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAg                                                                                              <  2:7647617/150‑1 (MQ=255)

                                                         ggCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAgg                                                                                             >  2:3394510/1‑150 (MQ=255)

                                                         ggCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAgg                                                                                             >  2:2143544/1‑150 (MQ=255)

                                                         ggCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAgg                                                                                             >  1:2356724/1‑150 (MQ=255)

                                                           ctctGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGtt                                                                                           <  1:1774517/150‑1 (MQ=255)

                                                              tgctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTc                                                                                                                                     >  2:6621659/1‑105 (MQ=255)

                                                              tgctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCa                                                                                        <  1:3692539/150‑1 (MQ=255)

                                                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCTGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                      <  1:3820502/150‑1 (MQ=255)

                                                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                      >  2:2963307/1‑150 (MQ=255)

                                                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                      >  2:874644/1‑150 (MQ=255)

                                                                 tgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                     >  2:2539320/1‑150 (MQ=255)

                                                                 tgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                     <  1:6472616/150‑1 (MQ=255)

                                                                 tgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                     >  1:9147825/1‑150 (MQ=255)

                                                                 tgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                     <  1:9034990/150‑1 (MQ=255)

                                                                   gCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGt                                                                                   <  1:6390791/150‑1 (MQ=255)

                                                                    cATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTg                                                                                  <  1:868725/150‑1 (MQ=255)

                                                                      ttCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGcc                                                                                >  1:417419/1‑150 (MQ=255)

                                                                      ttCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGcc                                                                                >  2:6308329/1‑150 (MQ=255)

                                                                      ttCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGcc                                                                                <  1:51821/150‑1 (MQ=255)

                                                                        cGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCt                                                                              <  2:8647003/150‑1 (MQ=255)

                                                                         gATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTg                                                                             >  2:5135317/1‑150 (MQ=255)

                                                                           tGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGa                                                                           <  2:7349150/150‑1 (MQ=255)

                                                                              tCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGa                                                                        <  1:8393492/150‑1 (MQ=255)

                                                                                aGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATg                                                                      >  2:5240637/1‑150 (MQ=255)

                                                                                aGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATg                                                                      <  1:6231969/150‑1 (MQ=255)

                                                                                 gCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGc                                                                     >  1:8466007/1‑150 (MQ=255)

                                                                                  cGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGcc                                                                    <  2:273171/150‑1 (MQ=255)

                                                                                   cagcaagcaCCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGccc                                                                   <  2:5896865/149‑1 (MQ=255)

                                                                                     gCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTg                                                                 <  2:6033910/150‑1 (MQ=255)

                                                                                      cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                <  1:7988581/150‑1 (MQ=255)

                                                                                      cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                >  2:2006815/1‑150 (MQ=255)

                                                                                      cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                >  1:7078174/1‑150 (MQ=255)

                                                                                      cAAGCACCAGCCATGACGGCTGCGAGAGGCTGTGCTCCGCCCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTgt                                                                <  2:4755473/150‑2 (MQ=255)

                                                                                       aaGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCTGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGc                                                               >  2:2388328/1‑150 (MQ=255)

                                                                                       aaGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGc                                                               >  1:1617485/1‑150 (MQ=255)

                                                                                       aaGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGc                                                               >  1:5041108/1‑150 (MQ=255)

                                                                                         gcaccagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTg                                                             >  2:7562762/4‑150 (MQ=255)

                                                                                             cagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGa                                                         >  1:3372669/1‑150 (MQ=255)

                                                                                                 cagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTa                                                     >  2:380243/1‑150 (MQ=255)

                                                                                                 cagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTa                                                     <  2:6492707/150‑1 (MQ=255)

                                                                                                   gggTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGa                                                   <  1:3673032/150‑1 (MQ=255)

                                                                                                    ggTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGAt                                                  >  2:7944411/1‑150 (MQ=255)

                                                                                                    ggTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGAt                                                  >  1:8717010/1‑150 (MQ=255)

                                                                                                             cAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGcc                                         >  2:1029035/1‑150 (MQ=255)

                                                                                                                  gCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcgg                                    <  1:5626829/150‑1 (MQ=255)

                                                                                                                  gCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcgg                                    <  1:2837000/150‑1 (MQ=255)

                                                                                                                    tgtgctccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTc                                  <  2:8543352/144‑1 (MQ=255)

                                                                                                                    tGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGt                                                      <  1:5904598/130‑1 (MQ=255)

                                                                                                                    tGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTc                                  >  2:6797181/1‑150 (MQ=255)

                                                                                                                      ggCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCAt                                <  2:409584/150‑1 (MQ=255)

                                                                                                                      ggCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCAt                                <  2:4490113/150‑1 (MQ=255)

                                                                                                                          ccgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCATTTCAGGCTGACGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTgg                            <  2:1888376/150‑1 (MQ=255)

                                                                                                                           cgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGc                           >  2:6969380/1‑150 (MQ=255)

                                                                                                                           cgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGc                           <  1:4094936/150‑1 (MQ=255)

                                                                                                                           cgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGc                           <  2:6132506/150‑1 (MQ=255)

                                                                                                                               cATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATa                       <  1:8083458/150‑1 (MQ=255)

                                                                                                                                aTGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATAc                      <  1:8965906/150‑1 (MQ=255)

                                                                                                                                aTGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATAc                      >  1:3912963/1‑150 (MQ=255)

                                                                                                                                  gccgccAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACAt                    <  2:4977158/150‑1 (MQ=255)

                                                                                                                                   ccgccAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACAtg                   <  1:4656734/150‑2 (MQ=255)

                                                                                                                                     gccCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTc                                  >  2:1468995/1‑133 (MQ=255)

                                                                                                                                     gccCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTc                                  <  1:1469253/133‑1 (MQ=255)

                                                                                                                                       ccAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCa               <  1:873914/150‑1 (MQ=255)

                                                                                                                                        cAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAg              <  2:7770814/150‑1 (MQ=255)

                                                                                                                                         aGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAga             >  1:6812368/1‑149 (MQ=255)

                                                                                                                                         aGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAga             <  1:1113172/150‑2 (MQ=255)

                                                                                                                                              aGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCtt        <  1:766251/150‑1 (MQ=255)

                                                                                                                                               gCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTg       <  1:277660/150‑1 (MQ=255)

                                                                                                                                                  gTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCg    <  1:7802555/150‑1 (MQ=255)

                                                                                                                                                    gAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTc  >  1:6557376/1‑150 (MQ=255)

CGTCGAGGTCAGTGGCCTGCTCGTCGACGTAGCGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTC  >  NC_002947/5738763‑5739060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`