BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,738,944	G→A	14.6%	H160H (CAC→CAT)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,738,944	0	G	A	14.6%	833.4 / 109.8	329	H160H (CAC→CAT)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base G (115/166); new base A (13/35); total (128/201)
Fisher's exact test for biased strand distribution p-value = 7.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

CGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGT  >  NC_002947/5738795‑5739092

    tttCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCt                                                                                                                                                  <  2:8637410/150‑1 (MQ=255)

    tttCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCt                                                                                                                                                  >  1:5479964/1‑150 (MQ=255)

     ttCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTg                                                                                                                                                 >  2:6831713/1‑150 (MQ=255)

           gCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGAcc                                                                                                                                           <  1:7213868/150‑1 (MQ=255)

             ggAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCgg                                                                                                                                         >  1:1333090/1‑150 (MQ=255)

                     aTACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTcgacg                                                                                                                                 >  2:9114241/1‑150 (MQ=255)

                      tACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTcgacga                                                                                                                                <  2:7619967/150‑1 (MQ=255)

                        cGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAg                                                                                                                              <  2:2102358/150‑1 (MQ=255)

                         ggCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAgg                                                                                                                             >  2:3394510/1‑150 (MQ=255)

                         ggCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAgg                                                                                                                             >  1:2356724/1‑150 (MQ=255)

                              tgctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCa                                                                                                                        <  1:3692539/150‑1 (MQ=255)

                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                                                      >  2:2963299/1‑150 (MQ=255)

                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                                                      <  1:8938265/150‑1 (MQ=255)

                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                                                      >  2:874644/1‑150 (MQ=255)

                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                                                      <  1:8738312/150‑1 (MQ=255)

                                ctgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGACGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATa                                                                                                                      >  1:3302423/1‑150 (MQ=255)

                                 tgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                                                     >  2:766251/1‑150 (MQ=255)

                                 tgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                                                     <  1:414682/150‑1 (MQ=255)

                                 tgGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAg                                                                                                                     <  1:6472616/150‑1 (MQ=255)

                                   tcATTCGATGTTCAGCGAGCAAGCCCAAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCGACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCTTAGGt                                                                                                                   >  2:5211524/2‑150 (MQ=255)

                                   gCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGt                                                                                                                   >  2:5050390/1‑150 (MQ=255)

                                    cATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTg                                                                                                                  <  1:868725/150‑1 (MQ=255)

                                      ttCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGcc                                                                                                                >  1:3590198/1‑150 (MQ=255)

                                        cGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCt                                                                                                              <  2:8647003/150‑1 (MQ=255)

                                           tGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGa                                                                                                           <  2:6291479/150‑1 (MQ=255)

                                           tGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGa                                                                                                           <  1:1607652/150‑1 (MQ=255)

                                                  cGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGcc                                                                                                    <  2:273171/150‑1 (MQ=255)

                                                      cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                                                >  2:2006814/1‑150 (MQ=255)

                                                      cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                                                <  2:1393222/150‑1 (MQ=255)

                                                      cAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTgg                                                                                                >  1:7078099/1‑150 (MQ=255)

                                                           cccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGcc                                                                                           >  1:3862347/1‑150 (MQ=255)

                                                           cccAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGcc                                                                                           >  1:765978/1‑150 (MQ=255)

                                                            ccagccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCg                                                                                          >  1:7715154/1‑150 (MQ=255)

                                                                ccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGt                                                                                      <  1:5892493/150‑1 (MQ=255)

                                                                ccagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGt                                                                                      <  2:3926690/150‑1 (MQ=255)

                                                                 cagGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTa                                                                                     <  2:6492707/150‑1 (MQ=255)

                                                                  agGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAg                                                                                    >  2:1541597/1‑150 (MQ=255)

                                                                  agGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAg                                                                                    <  1:9001102/150‑1 (MQ=255)

                                                                   gggTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGa                                                                                   <  1:3673061/150‑1 (MQ=255)

                                                                    ggTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGAt                                                                                  >  2:7944411/1‑150 (MQ=255)

                                                                      tCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATcc                                                                                <  1:6344159/150‑1 (MQ=255)

                                                                       cgcgGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCa                                                                               <  1:2783469/150‑1 (MQ=255)

                                                                        gcgGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAg                                                                              <  2:806790/150‑1 (MQ=255)

                                                                        gcgGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAg                                                                              >  1:9123486/1‑150 (MQ=255)

                                                                             cAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGcc                                                                         >  2:1029035/1‑150 (MQ=255)

                                                                                  gCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCcggcgg                                                                    <  1:5626820/150‑1 (MQ=255)

                                                                                   cTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGt                                                                   <  2:463724/150‑1 (MQ=255)

                                                                                    tgtgctccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTc                                                                  <  2:3816982/144‑1 (MQ=255)

                                                                                    tgtgctccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTc                                                                  <  2:8543352/144‑1 (MQ=255)

                                                                                    tGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGt                                                                                      >  2:5904598/1‑130 (MQ=255)

                                                                                    tGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTc                                                                  >  1:5297187/1‑150 (MQ=255)

                                                                                      ggCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGGAGTGCTGGCGACCGGCTTCGACGAAGGTGTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCag                                                                >  2:8145125/1‑149 (MQ=255)

                                                                                       gctccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATc                                                               <  1:1859847/147‑1 (MQ=255)

                                                                                         tccgccCATGCCGCCAAGGCCACCGGTCAGTATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTg                                                             <  2:1621897/149‑1 (MQ=255)

                                                                                         tccgccCATGCCGCCAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTg                                                             >  2:6058904/2‑150 (MQ=255)

                                                                                          ccgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTgg                                                            <  2:4957309/150‑1 (MQ=255)

                                                                                          ccgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTgg                                                            <  2:5516262/150‑1 (MQ=255)

                                                                                          ccgccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCATTTCAGGCTGACGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTgg                                                            <  2:1888376/150‑1 (MQ=255)

                                                                                            gccCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGcc                                                          >  2:2458512/1‑150 (MQ=255)

                                                                                               cATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATa                                                       <  1:8083347/150‑1 (MQ=255)

                                                                                                   ccgccAAGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACAtg                                                   <  1:4656734/150‑2 (MQ=255)

                                                                                                     gccCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTc                                                                  <  1:1469253/133‑1 (MQ=255)

                                                                                                       caaGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGcga                                               <  1:339731/148‑3 (MQ=255)

                                                                                                        cAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAg                                              <  1:4023757/150‑1 (MQ=255)

                                                                                                         aGGCCACCGGTCAGAATCCATTTGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAga                                             <  1:1113172/150‑2 (MQ=255)

                                                                                                          ggCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGc                                            <  1:4992421/150‑1 (MQ=255)

                                                                                                             cAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCt                                         <  1:1621628/150‑1 (MQ=255)

                                                                                                              aGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCtt                                        <  1:842187/150‑1 (MQ=255)

                                                                                                                  gTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCg                                    <  1:7802555/150‑1 (MQ=255)

                                                                                                                    gAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTc                                  >  1:6557376/1‑150 (MQ=255)

                                                                                                                     aGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTcc                                 <  1:679895/150‑1 (MQ=255)

                                                                                                                          ccACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTc                            >  1:6172835/1‑150 (MQ=255)

                                                                                                                             tttGCCCTTGAGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGGCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGtt                         <  2:1758650/149‑1 (MQ=255)

                                                                                                                              ttGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTg                        >  1:8318373/1‑150 (MQ=255)

                                                                                                                              ttGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTg                        >  1:6101305/1‑150 (MQ=255)

                                                                                                                                gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                      <  1:1309918/150‑1 (MQ=255)

                                                                                                                                gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                      >  2:3214978/1‑150 (MQ=255)

                                                                                                                                gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                      <  2:2180897/150‑1 (MQ=255)

                                                                                                                                gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                      <  1:5053159/150‑1 (MQ=255)

                                                                                                                                gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                      <  2:2073579/150‑1 (MQ=255)

                                                                                                                                gCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGAGGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                      <  2:6988153/150‑1 (MQ=255)

                                                                                                                                 cccttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGaaa                     <  2:4261989/144‑2 (MQ=255)

                                                                                                                                 cccttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGaaa                     <  2:6389138/144‑2 (MQ=255)

                                                                                                                                 cccttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGaaa                     <  2:6775921/144‑2 (MQ=255)

                                                                                                                                  ccttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGaaat                    >  2:6545544/6‑148 (MQ=255)

                                                                                                                                   tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                   >  2:944117/1‑150 (MQ=255)

                                                                                                                                   tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                   >  2:4614599/1‑150 (MQ=255)

                                                                                                                                   tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                   >  1:2414927/1‑150 (MQ=255)

                                                                                                                                   tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                   >  1:2842300/1‑150 (MQ=255)

                                                                                                                                   tttCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAgtg                   >  2:7427410/1‑150 (MQ=255)

                                                                                                                                   cttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAatg                   >  2:784589/5‑150 (MQ=255)

                                                                                                                                    ttgaGGTTGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAatga                  <  2:3581004/147‑2 (MQ=255)

                                                                                                                                     tCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGtt                 <  2:2656974/150‑1 (MQ=255)

                                                                                                                                         gCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGaa                      <  1:4400821/141‑1 (MQ=255)

                                                                                                                                         gCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCa             <  2:1321041/150‑1 (MQ=255)

                                                                                                                                             ccgccgTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTg         <  1:7854663/150‑1 (MQ=255)

                                                                                                                                             ccgccgTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTg         >  1:6923283/1‑150 (MQ=255)

                                                                                                                                               gccgTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGc       <  1:862633/150‑1 (MQ=255)

                                                                                                                                                  gTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCa    <  2:4417478/150‑1 (MQ=255)

                                                                                                                                                    aGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgt  <  1:2006815/150‑1 (MQ=255)

CGGGTTTCCAGGCGGAAGTCGATACGGCTCTGCTGGCATTCGATGTTCAGCGAGCAAGCCCCAGCCAGGGTCGCGGCCAGTGGCTGGGCGCCGCCCATGCCGCCCAGGCCAGCGGTGAGTACCCACTTGCCTTTCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGT  >  NC_002947/5738795‑5739092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`