BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,739,076	T→A	16.6%	E116D (GAA→GAT)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,739,076	0	T	A	16.6%	831.1 / 139.1	329	E116D (GAA→GAT)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base T (139/134); new base A (27/28); total (166/163)
Fisher's exact test for biased strand distribution p-value = 8.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 341 total aligned reads displayed.

TCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTT  >  NC_002947/5738928‑5739225

tCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGtt                                                                                                                                                      <  2:2656974/150‑1 (MQ=255)

    gCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCa                                                                                                                                                  <  2:1321041/150‑1 (MQ=255)

      tGCCGTCATAATGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGTTGTTAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAgg                                                                                                                                                <  2:6841301/150‑2 (MQ=255)

        ccgccgTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTg                                                                                                                                              >  1:6923283/1‑150 (MQ=255)

           ccgTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGcc                                                                                                                                           <  2:1617485/150‑1 (MQ=255)

             gTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCa                                                                                                                                         <  2:21591/150‑1 (MQ=255)

                 tGCTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAatgc                                                                                                                                     >  2:4741243/1‑149 (MQ=255)

                  gCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGtgtg                                                                                                                                    <  1:799805/150‑1 (MQ=255)

                         aCCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAg                                                                                                                             <  1:5911479/150‑1 (MQ=255)

                         aCCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAg                                                                                                                             >  2:5284035/1‑150 (MQ=255)

                           cGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGt                                                                                                                           >  2:5716296/1‑150 (MQ=255)

                           cGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGt                                                                                                                           >  2:3027938/1‑150 (MQ=255)

                            ggCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGtt                                                                                                                          >  2:5478275/1‑150 (MQ=255)

                            ggCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGAtt                                                                                                                          <  2:6607253/150‑1 (MQ=255)

                             gCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTg                                                                                                                         >  2:8878192/1‑150 (MQ=255)

                               ttCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGa                                                                                                                       <  2:7174128/150‑1 (MQ=255)

                               ttCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCGTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTAGGa                                                                                                                       >  1:3012924/1‑150 (MQ=255)

                                  gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                                                                                    >  1:7909040/1‑150 (MQ=255)

                                  gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                                                                                    >  2:7975206/1‑150 (MQ=255)

                                   acgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTg                                                                                                                   <  2:900014/150‑1 (MQ=255)

                                   acgaAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTg                                                                                                                   >  1:3355896/1‑150 (MQ=255)

                                    cgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGATGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTgg                                                                                                                  <  2:4853409/150‑1 (MQ=255)

                                     gaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGc                                                                                                                 >  2:1217329/1‑150 (MQ=255)

                                      aaGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCa                                                                                                                <  2:4241286/150‑1 (MQ=255)

                                       aGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGcga                                                                                                               <  1:941750/150‑3 (MQ=255)

                                         gTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATc                                                                                                             <  1:9086176/150‑1 (MQ=255)

                                          tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                                                                                            <  1:4198478/150‑1 (MQ=255)

                                          tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                                                                                            <  1:4810025/150‑1 (MQ=255)

                                          tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                                                                                            >  1:524123/1‑150 (MQ=255)

                                          tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                                                                                            <  2:7715154/150‑1 (MQ=255)

                                          tttCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGAtga                                                                                                            <  2:448412/150‑3 (MQ=255)

                                              aTAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAc                                                                                                        >  2:7565309/1‑150 (MQ=255)

                                              aTAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAc                                                                                                        >  1:1018377/1‑150 (MQ=255)

                                                  gTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAAcgcgc                                                                                                    <  2:2894319/150‑1 (MQ=255)

                                                     cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                                                                                                 >  1:1874383/1‑150 (MQ=255)

                                                     cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                                                                                                 >  1:3933214/1‑150 (MQ=255)

                                                     cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                                                                                                 <  1:2963299/150‑1 (MQ=255)

                                                     cccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGt                                                                                                 >  2:1495742/1‑150 (MQ=255)

                                                     cccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGt                                                                                                 <  2:400734/150‑1 (MQ=255)

                                                     cccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGt                                                                                                 <  2:914794/150‑1 (MQ=255)

                                                      ccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTg                                                                                                >  2:6017949/1‑150 (MQ=255)

                                                        tGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCg                                                                                              >  1:2171270/1‑150 (MQ=255)

                                                                gCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCtgtg                                                                                      >  1:3039362/1‑150 (MQ=255)

                                                                 cccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTgg                                                                                     <  2:3372669/150‑1 (MQ=255)

                                                                 cccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTgg                                                                                     >  2:3751080/1‑150 (MQ=255)

                                                                 cccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTgg                                                                                     <  1:2388328/150‑1 (MQ=255)

                                                                  ccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTg                                                                                                                                              >  1:85979/1‑92 (MQ=255)

                                                                   cTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGt                                                                                   >  1:1753879/1‑150 (MQ=255)

                                                                       gcaccGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCACGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtgcgg                                                                               <  2:6801535/147‑5 (MQ=255)

                                                                       cTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTg                                                                               >  1:2330489/1‑150 (MQ=255)

                                                                         gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                             <  1:4547579/150‑1 (MQ=255)

                                                                         gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                             <  1:5050390/150‑1 (MQ=255)

                                                                         gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                             <  2:2356724/150‑1 (MQ=255)

                                                                         accGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtgcggaa                                                                             <  2:1350610/149‑7 (MQ=255)

                                                                         accGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtgcggaa                                                                             >  2:1741087/2‑144 (MQ=255)

                                                                            gATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTgg                                                                                                                                   <  1:5499955/93‑1 (MQ=255)

                                                                                  gATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgac                                                                    >  1:8743523/1‑150 (MQ=255)

                                                                                   aTCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgacc                                                                   <  2:417419/150‑1 (MQ=255)

                                                                                    tCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgaccg                                                                  >  1:77712/1‑150 (MQ=255)

                                                                                       aGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCt                                                               >  1:4509742/1‑150 (MQ=255)

                                                                                            ccGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGccc                                                          >  2:8177835/1‑150 (MQ=255)

                                                                                               gcggTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGAc                                                       <  1:768010/150‑1 (MQ=255)

                                                                                               gcggTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGAc                                                       <  1:6308329/150‑1 (MQ=255)

                                                                                                    cATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCAc                                                  <  1:4715014/150‑1 (MQ=255)

                                                                                                     aTCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCAcc                                                 >  2:3614179/1‑150 (MQ=255)

                                                                                                      tCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACca                                                <  1:2016347/150‑1 (MQ=255)

                                                                                                      ccTGGCGATGTATGGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGtt                                                                                                                          >  1:6481088/2‑76 (MQ=255)

                                                                                                        tGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGTCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACcagc                                              >  1:2697894/1‑150 (MQ=255)

                                                                                                        tGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACcagc                                              >  1:7475676/1‑150 (MQ=255)

                                                                                                           ccATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                           <  2:8717010/150‑1 (MQ=255)

                                                                                                           ccATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                           <  2:8318373/150‑1 (MQ=255)

                                                                                                                cATCGCGAGACCCTTCTTATCCAGCTCGATGAAATGATCCCAGCTCGCCCAATGCGGCACGAGAATGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTtc                                      <  2:5168132/150‑1 (MQ=255)

                                                                                                                 aTCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcg                                     >  1:2062489/1‑150 (MQ=255)

                                                                                                                  tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgt                                    <  1:1470742/150‑1 (MQ=255)

                                                                                                                  tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGACAGACTGCACCAGCAGGGTTtcgt                                    >  2:85559/1‑150 (MQ=255)

                                                                                                                   cGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgtc                                   <  2:7078099/150‑1 (MQ=255)

                                                                                                                   cGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgtc                                   <  1:3214978/150‑1 (MQ=255)

                                                                                                                      cAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCgtcgtc                                >  1:5204336/1‑150 (MQ=255)

                                                                                                                       aGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCt                               <  1:6795487/150‑1 (MQ=255)

                                                                                                                       aGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCt                               <  2:5500401/150‑1 (MQ=255)

                                                                                                                         gCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTc                             >  2:5234561/1‑150 (MQ=255)

                                                                                                                         gCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTc                             <  2:6562099/150‑1 (MQ=255)

                                                                                                                           ccTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCa                           >  2:1857053/1‑150 (MQ=255)

                                                                                                                            cTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAg                          <  1:8145125/150‑1 (MQ=255)

                                                                                                                                gCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                      <  2:6101305/150‑1 (MQ=255)

                                                                                                                                gCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCgg                      <  1:2571301/150‑1 (MQ=255)

                                                                                                                                  gTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGt                    <  1:5284035/150‑1 (MQ=255)

                                                                                                                                  gTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGt                    >  1:3669992/1‑150 (MQ=255)

                                                                                                                                       gTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAggg               >  1:7310282/1‑150 (MQ=255)

                                                                                                                                          cGTTGTAGTGGTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGttt            <  2:7820081/150‑1 (MQ=255)

                                                                                                                                          cGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGttt            <  2:4435656/150‑1 (MQ=255)

                                                                                                                                             tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga         <  2:1579236/150‑1 (MQ=255)

                                                                                                                                             tGAAGTGTTCCAAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga         >  1:8207131/1‑150 (MQ=255)

                                                                                                                                              gAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGTCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGAACGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCTGGGTTTcgac        <  2:3239642/150‑1 (MQ=255)

                                                                                                                                              gAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcgac        >  1:5094144/1‑150 (MQ=255)

                                                                                                                                                 gtgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGAt     >  2:6427351/1‑150 (MQ=255)

                                                                                                                                                 gtgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGAt     >  1:7850185/1‑150 (MQ=255)

                                                                                                                                                  tgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATc    >  1:1356085/1‑150 (MQ=255)

                                                                                                                                                    ttCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCtt  >  2:5827863/1‑150 (MQ=255)

                                                                                                                                                    ttCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCtt  >  1:4766991/1‑150 (MQ=255)

TCAGGCTGCCGCCGTAGTGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTT  >  NC_002947/5738928‑5739225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`